FastQCFastQC Report
Thu 26 May 2016
SRR1544700_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544700_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1880044
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103120.5484978011152931No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA58450.3108969790068743No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA24730.13153947460804108No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCCG206.3124823E-446.00000432
TTCGTCA206.3124823E-446.00000413
CGTTAAG206.3124823E-446.00000427
TGTTGCG206.3124823E-446.0000041
TACGCTA206.3124823E-446.00000419
CGCTCGA405.6152203E-946.00000426
GCCGAAC206.3124823E-446.00000423
TGCGCAT206.3124823E-446.00000423
CGCTAAT206.3124823E-446.00000443
ACTACGA206.3124823E-446.00000445
CTACCTA206.3124823E-446.00000436
CGTAACG206.3124823E-446.00000428
CGTCGGA405.6152203E-946.00000425
TATACGC206.3124823E-446.00000415
TACGTAA405.6152203E-946.00000445
CCCGATA405.6152203E-946.00000424
TAATCGC206.3124823E-446.00000423
GATCGGA301.8620394E-646.013
CGACGTG253.417991E-546.016
AGATACG1300.046.01
Produced by FastQC (version 0.11.5)