##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544700_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1880044
Sequences flagged as poor quality	0
Sequence length	52
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.68060428372953	33.0	31.0	34.0	31.0	34.0
2	33.095739780558326	34.0	33.0	34.0	31.0	34.0
3	33.14235251940912	34.0	33.0	34.0	31.0	34.0
4	36.47684043564938	37.0	37.0	37.0	35.0	37.0
5	36.47316605356045	37.0	37.0	37.0	35.0	37.0
6	36.535906606441124	37.0	37.0	37.0	35.0	37.0
7	36.611784085904375	37.0	37.0	37.0	35.0	37.0
8	36.60225771311735	37.0	37.0	37.0	35.0	37.0
9	38.28931982442964	39.0	39.0	39.0	37.0	39.0
10	38.220188463674255	39.0	39.0	39.0	37.0	39.0
11	38.05158123958801	39.0	39.0	39.0	35.0	39.0
12	37.3695631591601	39.0	35.0	39.0	35.0	39.0
13	37.25884287814541	39.0	35.0	39.0	35.0	39.0
14	38.453128756561014	40.0	35.0	41.0	35.0	41.0
15	38.470467180555346	40.0	35.0	41.0	35.0	41.0
16	38.52226809585308	40.0	35.0	41.0	35.0	41.0
17	38.48047652076228	40.0	35.0	41.0	35.0	41.0
18	38.50494030990764	40.0	36.0	41.0	35.0	41.0
19	38.469658688839196	40.0	36.0	41.0	35.0	41.0
20	38.33046035092796	40.0	35.0	41.0	35.0	41.0
21	38.26190078530077	40.0	35.0	41.0	35.0	41.0
22	38.18816048986088	40.0	35.0	41.0	35.0	41.0
23	38.11212503537151	40.0	35.0	41.0	35.0	41.0
24	38.04863715955584	40.0	35.0	41.0	35.0	41.0
25	38.04788132618173	40.0	35.0	41.0	35.0	41.0
26	38.00394352472602	40.0	35.0	41.0	35.0	41.0
27	37.965217303424815	40.0	35.0	41.0	35.0	41.0
28	37.93740784790143	40.0	35.0	41.0	35.0	41.0
29	37.9037065089966	40.0	35.0	41.0	35.0	41.0
30	37.847039750133504	40.0	35.0	41.0	35.0	41.0
31	37.7550323290306	40.0	35.0	41.0	35.0	41.0
32	37.636616483444	40.0	35.0	41.0	35.0	41.0
33	37.51637514866673	40.0	35.0	41.0	34.0	41.0
34	37.376451295820736	40.0	35.0	41.0	34.0	41.0
35	37.24904895842863	40.0	35.0	41.0	34.0	41.0
36	37.141913167989685	40.0	35.0	41.0	34.0	41.0
37	37.09543978757944	40.0	35.0	41.0	34.0	41.0
38	36.975091008508315	40.0	35.0	41.0	33.0	41.0
39	36.97110493158671	39.0	35.0	41.0	33.0	41.0
40	36.84822802019527	39.0	35.0	41.0	33.0	41.0
41	36.834461853020464	39.0	35.0	41.0	33.0	41.0
42	36.803513109267655	39.0	35.0	41.0	33.0	41.0
43	36.7618358932025	39.0	35.0	41.0	33.0	41.0
44	36.65892021676088	39.0	35.0	41.0	33.0	41.0
45	36.59578233275391	39.0	35.0	41.0	33.0	41.0
46	36.53526619589754	39.0	35.0	41.0	33.0	41.0
47	36.48795187772201	38.0	35.0	41.0	33.0	41.0
48	36.43446695928393	38.0	35.0	41.0	33.0	41.0
49	36.38476333532619	38.0	35.0	41.0	33.0	41.0
50	36.28225828757199	38.0	35.0	41.0	33.0	41.0
51	36.225250047339316	38.0	35.0	40.0	32.0	41.0
52	36.00094359493714	37.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	2.0
12	6.0
13	7.0
14	6.0
15	27.0
16	47.0
17	136.0
18	307.0
19	546.0
20	999.0
21	1598.0
22	2593.0
23	4010.0
24	6550.0
25	10430.0
26	14592.0
27	16640.0
28	16621.0
29	16579.0
30	17710.0
31	20890.0
32	26361.0
33	36469.0
34	95591.0
35	380501.0
36	75726.0
37	99643.0
38	186707.0
39	845428.0
40	3318.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.53261944933203	23.520992061887913	28.920014637955283	14.026373850824768
2	31.52665575912053	25.39270357502271	29.565265493786313	13.51537517207044
3	28.86783500811683	25.4223305412001	32.2069057958218	13.502928654861268
4	26.339223975609084	28.226254279155167	31.435540870320054	13.998980874915693
5	23.555193389090896	33.62368114788803	28.8881004912651	13.93302497175598
6	22.470006021135674	41.792372944463004	25.552912591407434	10.184708442993887
7	87.49683518045323	4.536595952009634	6.203897355593805	1.7626715119433374
8	87.79810472520856	3.668371591303182	6.502401007635991	2.0311226758522674
9	83.22007357274617	5.991402328881665	7.623757741840084	3.164766356532081
10	46.69071574920587	28.59837322956271	13.313890525966412	11.397020495265005
11	41.80859596903051	21.110091040422457	23.00015318790411	14.081159802642917
12	39.39875875245473	20.966796521783532	24.95776694587999	14.676677779881746
13	20.22447346976986	38.186446700183616	26.05524126031093	15.533838569735604
14	15.467031622664152	39.32232437113174	28.504864779760474	16.70577922644364
15	14.826089176636293	24.875002925463445	45.40228845707867	14.896619440821599
16	16.572963185967986	21.1466859286272	43.56323575405682	18.717115131347988
17	16.377648608224064	21.891402541642645	29.628455504232882	32.10249334590041
18	19.651561346436573	24.03268221382053	36.85530764173605	19.460448798006855
19	26.463210435500446	24.507777477548398	29.157030367374382	19.871981719576777
20	29.168785411405263	22.799147254000438	29.205380299610006	18.82668703498429
21	20.446489550244568	28.47204639891407	30.420458244594272	20.661005806247086
22	21.64805717312999	23.86555846565293	26.279916853009823	28.206467508207254
23	17.35762567259064	29.91094889268549	27.697170917276402	25.03425451744746
24	16.890615326024285	24.23815612826083	42.14720506541336	16.724023480301526
25	16.162387688798773	27.313243732593495	35.96926454912757	20.555104029480162
26	16.36312767148003	34.05558593309518	28.35678313911802	21.224503256306768
27	16.609664454661697	36.415583890589794	28.63885100561476	18.335900649133745
28	15.171666195046498	30.307641736044477	36.37930814385195	18.141383925057074
29	16.412009506160494	24.490171506624314	34.929182508494485	24.16863647872071
30	17.444857673543808	33.34560254972756	30.75454617019602	18.454993606532614
31	26.404169264123606	29.627604460321145	26.614962203012272	17.353264072542984
32	27.98354719357632	25.860139443544938	28.529864194667788	17.626449168210957
33	26.502677596907308	28.237956132941573	26.50884766526741	18.750518604883716
34	19.28805921563538	27.47265489531096	28.36332553918951	24.87596034986415
35	19.932884549510543	26.132367114812205	32.46971879381547	21.465029541861785
36	28.95958817985111	27.425900670409842	26.806181131930956	16.808330017808093
37	18.995672441708813	35.242260287525184	29.698985768418183	16.063081502347817
38	19.586137345721696	34.318984023778164	26.83575490786386	19.25912372263628
39	21.15413256285491	33.04358834154945	28.06077942856657	17.74149966702907
40	24.65133794740974	26.997506441338608	27.860305397107727	20.490850214143926
41	17.12039718219361	26.616185578635392	30.082327860411777	26.18108937875922
42	19.48167170555583	25.979019639965873	27.540632027761053	26.99867662671725
43	20.869245613400537	25.156858030982253	28.982353604490108	24.991542751127103
44	17.227309573605723	29.941426902774616	30.47896751352628	22.35229601009338
45	16.621100357225682	38.260593900993804	24.581286395424787	20.537019346355724
46	20.118039790558093	34.491958698838964	26.55416575356747	18.835835757035476
47	19.341249460119016	28.977460101997615	28.88432398390676	22.79696645397661
48	20.537232107333658	26.00604028416356	34.08420228462738	19.372525323875397
49	21.196365616974923	26.362414922203946	33.54708719583159	18.894132264989544
50	19.40922659256911	33.27677437336573	29.001608472993183	18.312390561071975
51	17.678150085849055	32.454187242426244	27.28909536159792	22.578567310126786
52	18.314624551340287	27.971366627589568	34.90939573754657	18.804613083523577
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6963.0
1	7068.5
2	7174.0
3	12471.0
4	17768.0
5	11987.5
6	6207.0
7	6491.5
8	6776.0
9	7243.5
10	7711.0
11	7808.5
12	7906.0
13	7738.0
14	6951.0
15	6332.0
16	6088.5
17	5845.0
18	6093.0
19	6341.0
20	6050.5
21	5760.0
22	7361.0
23	8962.0
24	11142.0
25	13322.0
26	14932.0
27	16542.0
28	23769.0
29	30996.0
30	35346.5
31	39697.0
32	41751.0
33	43805.0
34	53841.0
35	63877.0
36	64887.5
37	65898.0
38	72449.5
39	104264.5
40	129528.0
41	155231.0
42	180934.0
43	192481.0
44	204028.0
45	195983.0
46	187938.0
47	175895.5
48	163853.0
49	145176.5
50	126500.0
51	111506.0
52	96512.0
53	85207.5
54	73903.0
55	65725.0
56	57547.0
57	51845.0
58	46143.0
59	44224.5
60	42306.0
61	38916.0
62	35526.0
63	29907.5
64	21323.0
65	18357.0
66	15102.0
67	11847.0
68	10313.0
69	8779.0
70	7495.5
71	6212.0
72	5326.5
73	4441.0
74	3669.5
75	2898.0
76	2385.5
77	1873.0
78	1410.0
79	947.0
80	819.5
81	692.0
82	448.0
83	204.0
84	173.0
85	142.0
86	136.5
87	131.0
88	88.0
89	27.5
90	10.0
91	6.5
92	3.0
93	2.0
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1880044.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.861633927222806
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.51396071427163	11.371224744574123
2	9.571638949874625	2.8450038831316804
3	3.350635792936289	1.4938776773420714
4	1.578413414171436	0.938312093889352
5	0.9207423085947979	0.6841867565820949
6	0.6009563135833075	0.5358715643237058
7	0.46519376321454664	0.48394775898852316
8	0.3484532880052173	0.41428681656565425
9	0.28087349428388697	0.37568151467163324
>10	3.6177136185809395	13.34984706246699
>50	1.2059285639822515	12.61961948390073
>100	1.5156026276120167	51.11332170685831
>500	0.027366547801975078	2.427533196388025
>1k	0.0018004307764457289	0.48264895503336697
>5k	3.6008615528914577E-4	0.31279334096574235
>10k+	3.6008615528914577E-4	0.5518434443180043
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10312	0.5484978011152931	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5845	0.3108969790068743	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	2473	0.13153947460804108	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09877428400611901	0.0
2	5.3190244483639744E-5	0.0	0.0	0.2945675739503969	0.0
3	5.3190244483639744E-5	0.0	0.0	0.4086074581233205	0.0
4	5.3190244483639744E-5	0.0	0.0	0.55839118658925	0.0
5	5.3190244483639744E-5	0.0	0.0	0.9759877960303057	0.0
6	5.3190244483639744E-5	0.0	0.0	1.3068843069630285	0.0
7	5.3190244483639744E-5	0.0	0.0	1.5110284652912378	0.0
8	5.3190244483639744E-5	0.0	0.0	1.9686773288284742	0.0
9	5.3190244483639744E-5	0.0	0.0	2.1370776428636775	0.0
10	5.3190244483639744E-5	0.0	0.0	2.4587722415007307	0.0
11	5.3190244483639744E-5	0.0	0.0	2.885943094948842	0.0
12	5.3190244483639744E-5	0.0	0.0	3.2270521328224233	0.0
13	1.0638048896727949E-4	0.0	0.0	3.3632723489450247	0.0
14	1.0638048896727949E-4	0.0	0.0	3.409281910423373	0.0
15	1.0638048896727949E-4	0.0	0.0	3.488163042992611	0.0
16	1.0638048896727949E-4	0.0	0.0	3.663584469299655	0.0
17	1.0638048896727949E-4	0.0	0.0	3.872568939875875	0.0
18	1.0638048896727949E-4	0.0	0.0	4.160594113754785	0.0
19	1.0638048896727949E-4	0.0	0.0	4.297239851833255	0.0
20	1.0638048896727949E-4	0.0	0.0	4.445374682720192	0.0
21	1.0638048896727949E-4	0.0	0.0	4.65286982645087	0.0
22	1.0638048896727949E-4	0.0	0.0	4.858662882358073	0.0
23	1.0638048896727949E-4	0.0	0.0	5.089880875128454	0.0
24	1.5957073345091924E-4	0.0	0.0	5.275674399109808	0.0
25	1.5957073345091924E-4	0.0	0.0	5.419075298237701	0.0
26	1.5957073345091924E-4	0.0	0.0	5.568486694992245	0.0
27	1.5957073345091924E-4	0.0	0.0	5.716355574656763	0.0
28	1.5957073345091924E-4	0.0	0.0	5.865235068966471	0.0
29	2.1276097793455898E-4	0.0	0.0	6.032677958600969	0.0
30	2.1276097793455898E-4	0.0	0.0	6.218099150870937	0.0
31	2.659512224181987E-4	0.0	0.0	6.398945982115312	0.0
32	2.659512224181987E-4	0.0	0.0	6.564739974170817	0.0
33	2.659512224181987E-4	0.0	0.0	6.737767839476097	0.0
34	3.191414669018385E-4	0.0	0.0	6.907976621823744	0.0
35	3.191414669018385E-4	0.0	0.0	7.128822516919817	0.0
36	3.191414669018385E-4	0.0	0.0	7.320945679994724	0.0
37	3.191414669018385E-4	0.0	0.0	7.498707477059048	0.0
38	3.191414669018385E-4	0.0	0.0	7.690458308422569	0.0
39	3.191414669018385E-4	0.0	0.0	7.879336866583974	0.0
40	3.191414669018385E-4	0.0	0.0	8.10374650806045	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCCCG	20	6.3124823E-4	46.000004	32
TTCGTCA	20	6.3124823E-4	46.000004	13
CGTTAAG	20	6.3124823E-4	46.000004	27
TGTTGCG	20	6.3124823E-4	46.000004	1
TACGCTA	20	6.3124823E-4	46.000004	19
CGCTCGA	40	5.6152203E-9	46.000004	26
GCCGAAC	20	6.3124823E-4	46.000004	23
TGCGCAT	20	6.3124823E-4	46.000004	23
CGCTAAT	20	6.3124823E-4	46.000004	43
ACTACGA	20	6.3124823E-4	46.000004	45
CTACCTA	20	6.3124823E-4	46.000004	36
CGTAACG	20	6.3124823E-4	46.000004	28
CGTCGGA	40	5.6152203E-9	46.000004	25
TATACGC	20	6.3124823E-4	46.000004	15
TACGTAA	40	5.6152203E-9	46.000004	45
CCCGATA	40	5.6152203E-9	46.000004	24
TAATCGC	20	6.3124823E-4	46.000004	23
GATCGGA	30	1.8620394E-6	46.0	13
CGACGTG	25	3.417991E-5	46.0	16
AGATACG	130	0.0	46.0	1
>>END_MODULE