FastQCFastQC Report
Thu 26 May 2016
SRR1544699_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544699_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1848470
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96480.5219451762809242No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60020.3247009689094224No Hit
CTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCTT21100.11414845791384226Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT20570.11128122176719125No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAT351.0200165E-746.00000427
GGACGTA700.046.0000048
TATCGTG351.0200165E-746.0000041
GGTCGTA301.862023E-646.022
CTATCGA253.4179688E-546.032
TCACACG253.4179688E-546.01
CGGTTAG301.862023E-646.035
TTATCCG206.312456E-446.01
ATCCGTT206.312456E-446.046
ACGATAC501.6370905E-1146.035
CCCGTAC206.312456E-446.023
TGCGATA206.312456E-446.025
CGGGCTA453.110472E-1046.06
ATAACCG206.312456E-446.041
GCGGAAC301.862023E-646.016
CGCAACA253.4179688E-546.017
TATCGAA301.862023E-646.035
TCGAATC206.312456E-446.033
TACGTCC453.110472E-1046.043
TATCCGA405.6152203E-946.039
Produced by FastQC (version 0.11.5)