##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544699_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1848470
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.64704647627497	33.0	31.0	34.0	31.0	34.0
2	33.071601919425255	34.0	33.0	34.0	31.0	34.0
3	33.11593696408381	34.0	33.0	34.0	31.0	34.0
4	36.46023413958571	37.0	37.0	37.0	35.0	37.0
5	36.462630175225996	37.0	37.0	37.0	35.0	37.0
6	36.52487733098184	37.0	37.0	37.0	35.0	37.0
7	36.60089695802475	37.0	37.0	37.0	35.0	37.0
8	36.59162442452406	37.0	37.0	37.0	35.0	37.0
9	38.286150708423726	39.0	39.0	39.0	37.0	39.0
10	38.22019670321942	39.0	39.0	39.0	37.0	39.0
11	38.063160884407104	39.0	39.0	39.0	35.0	39.0
12	37.41310489215405	39.0	35.0	39.0	35.0	39.0
13	37.31734894263905	39.0	35.0	39.0	35.0	39.0
14	38.50794116215032	40.0	35.0	41.0	35.0	41.0
15	38.52911488961141	40.0	35.0	41.0	35.0	41.0
16	38.57611808685021	40.0	35.0	41.0	35.0	41.0
17	38.53510579019405	40.0	35.0	41.0	35.0	41.0
18	38.53997197682408	40.0	36.0	41.0	35.0	41.0
19	38.498187149372185	40.0	36.0	41.0	35.0	41.0
20	38.351159066687586	40.0	35.0	41.0	35.0	41.0
21	38.28476740222995	40.0	35.0	41.0	35.0	41.0
22	38.208122934102256	40.0	35.0	41.0	35.0	41.0
23	38.12154863211196	40.0	35.0	41.0	35.0	41.0
24	38.07910001244272	40.0	35.0	41.0	35.0	41.0
25	38.069596477086456	40.0	35.0	41.0	35.0	41.0
26	38.00426785395489	40.0	35.0	41.0	35.0	41.0
27	37.95820543476497	40.0	35.0	41.0	35.0	41.0
28	37.929879846575815	40.0	35.0	41.0	35.0	41.0
29	37.89844574161334	40.0	35.0	41.0	35.0	41.0
30	37.84204666562076	40.0	35.0	41.0	35.0	41.0
31	37.71210081851477	40.0	35.0	41.0	35.0	41.0
32	37.57633015412747	40.0	35.0	41.0	34.0	41.0
33	37.440338766655664	40.0	35.0	41.0	34.0	41.0
34	37.29801673816724	40.0	35.0	41.0	34.0	41.0
35	37.17587951116329	40.0	35.0	41.0	34.0	41.0
36	37.075822166440354	40.0	35.0	41.0	33.0	41.0
37	37.030941805925984	40.0	35.0	41.0	33.0	41.0
38	36.88707958473765	40.0	35.0	41.0	33.0	41.0
39	36.89360768635682	39.0	35.0	41.0	33.0	41.0
40	36.79833267513133	39.0	35.0	41.0	33.0	41.0
41	36.761679118406036	39.0	35.0	41.0	33.0	41.0
42	36.726134586982745	39.0	35.0	41.0	33.0	41.0
43	36.66191661211705	39.0	35.0	41.0	33.0	41.0
44	36.5491806737464	39.0	35.0	41.0	33.0	41.0
45	36.480326432130354	39.0	35.0	41.0	33.0	41.0
46	36.43183389505916	39.0	35.0	41.0	32.0	41.0
47	36.37293653670333	38.0	35.0	41.0	32.0	41.0
48	36.297957229492496	38.0	35.0	41.0	32.0	41.0
49	36.25445747023214	38.0	35.0	41.0	32.0	41.0
50	36.11443464054056	38.0	35.0	41.0	32.0	41.0
51	36.04687877000979	38.0	35.0	40.0	32.0	41.0
52	35.81703516962677	37.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	6.0
12	5.0
13	11.0
14	15.0
15	27.0
16	62.0
17	128.0
18	257.0
19	582.0
20	1008.0
21	1638.0
22	2818.0
23	4465.0
24	7315.0
25	11307.0
26	15326.0
27	17399.0
28	17422.0
29	17365.0
30	18427.0
31	21228.0
32	26949.0
33	35916.0
34	90269.0
35	362403.0
36	78743.0
37	97267.0
38	187600.0
39	829518.0
40	2991.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.55131541220577	23.86027363170622	28.215443042083454	14.372967914004557
2	31.45401331912338	26.003181009158926	29.487630310472984	13.055175361244705
3	28.79949363527674	25.009277943380198	32.71803166943472	13.473196751908334
4	26.577061028850892	27.474668239138317	31.156578143004754	14.791692589006042
5	23.255016310786758	33.395510881972655	28.84866944013157	14.500803367109013
6	21.469972463713233	42.87069846954508	25.069868594026413	10.589460472715274
7	87.03495323159153	4.825342039632777	6.345247691333913	1.794457037441776
8	87.22273014979956	3.9407185401981097	6.962731339973059	1.8738199700292675
9	82.7239825369089	5.877780001839359	8.132942379373212	3.2652950818785267
10	45.16005128565787	30.179337506153736	13.674660665306984	10.98595054288141
11	40.74115349451168	22.20176686665188	23.003240517833667	14.053839121002776
12	37.801370863470865	21.9972193219257	25.27030463031588	14.931105184287546
13	21.20629493581178	37.9782198250445	25.04530774099661	15.770177498147117
14	15.101570488025231	39.08832710295542	29.219624878953947	16.590477530065407
15	15.290591678523318	25.837746893376686	43.71739871353065	15.154262714569347
16	17.078232267767397	21.223336056306024	43.121879175750756	18.576552500175822
17	17.350511504108805	21.347925581697297	30.351317576157577	30.950245338036318
18	19.390468874258172	24.209968244007207	36.840900853138	19.558662028596626
19	27.357273853511284	24.287816410328542	28.582286972469124	19.772622763691054
20	28.957408018523427	24.091113190909237	29.58857866235319	17.362900128214147
21	21.261475706936007	29.357901399535834	30.61045080526057	18.770172088267596
22	20.943374791043404	24.526013405681454	28.092260085367897	26.438351717907242
23	17.462279615032976	30.81072454516438	28.12012096490611	23.606874874896537
24	17.214939923287908	23.521182383268325	41.422419622715	17.841458070728766
25	16.896568513419204	26.587393898737876	37.00444151108755	19.51159607675537
26	16.848745178444876	34.40477800559382	28.739552170173173	20.00692464578814
27	17.03884834484736	35.48529324251949	28.776609844898754	18.699248567734397
28	15.191212191704489	29.539078264727042	37.69652739833484	17.573182145233627
29	16.573815101137697	25.230650213419747	36.57798070836962	21.61755397707293
30	18.355126131341056	33.37798287232143	29.749847170903504	18.51704382543401
31	27.216616985939723	28.378821403647343	26.406217033546664	17.998344576866273
32	28.450123615747074	25.466953750939968	28.910558461863054	17.172364171449903
33	27.139526202751462	27.55251640545965	25.756652799342163	19.551304592446726
34	19.091464833078167	28.367406557855958	27.48386503432568	25.05726357474019
35	19.973599787932724	26.32701639734483	32.37802074147809	21.32136307324436
36	28.070999258846506	27.34742787278127	26.530103274600076	18.051469593772147
37	18.54842112666151	36.13658863817103	28.66657289542162	16.648417339745844
38	18.914940464275862	34.450220993578476	26.813418665166328	19.82141987697934
39	20.736230504146672	33.15693519505321	27.15478206300346	18.952052237796664
40	25.354698750858816	26.13069186949207	27.356029581221225	21.15857979842789
41	17.46958295238765	26.523286826402376	28.285663278278793	27.721466942931183
42	19.38770983570196	25.272793174895998	27.39703646799786	27.94246052140419
43	19.48021877552787	24.935216692724254	29.480976158660948	26.103588373086932
44	17.430036733081955	28.827084020838857	32.5340957656873	21.20878348039189
45	16.546927999913443	36.58658241680958	26.56921670354401	20.29727287973297
46	19.606485363570954	33.960735094429445	27.153213197942083	19.279566344057518
47	18.994627989634672	28.884969731724077	28.901740358242222	23.218661920399033
48	19.923882995125698	26.33415743831385	34.9075181095717	18.834441456988753
49	21.047298576660697	26.301265370820193	33.526862756766405	19.124573295752704
50	19.47004820202654	34.38649261280951	28.48902064951013	17.65443853565381
51	18.46435159888989	33.586209135122566	26.418930250423323	21.530509015564224
52	19.599073828625837	27.26828133537466	34.108587101765245	19.02405773423426
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7029.0
1	6943.5
2	6858.0
3	12508.5
4	18159.0
5	12530.5
6	6902.0
7	7129.5
8	7357.0
9	7684.5
10	8012.0
11	7895.0
12	7778.0
13	7566.5
14	7138.5
15	6922.0
16	6982.5
17	7043.0
18	7166.5
19	7290.0
20	7206.5
21	7123.0
22	9776.0
23	12429.0
24	14180.5
25	15932.0
26	16674.0
27	17416.0
28	21869.0
29	26322.0
30	32073.0
31	37824.0
32	39089.5
33	40355.0
34	50586.0
35	60817.0
36	65766.0
37	70715.0
38	70931.5
39	98839.5
40	126531.0
41	155320.5
42	184110.0
43	188376.5
44	192643.0
45	182202.0
46	171761.0
47	162096.5
48	152432.0
49	141346.0
50	130260.0
51	115748.5
52	101237.0
53	90282.0
54	79327.0
55	69841.5
56	60356.0
57	53681.5
58	47007.0
59	45048.0
60	43089.0
61	36728.5
62	30368.0
63	27089.0
64	20137.0
65	16464.0
66	13816.5
67	11169.0
68	9579.0
69	7989.0
70	7273.5
71	6558.0
72	5632.0
73	4706.0
74	4079.5
75	3453.0
76	2779.5
77	2106.0
78	1497.5
79	889.0
80	859.5
81	830.0
82	589.5
83	349.0
84	244.0
85	139.0
86	89.0
87	39.0
88	26.0
89	28.0
90	43.0
91	22.5
92	2.0
93	2.0
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1848470.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.678169536175535
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.09017120709045	12.086327737575617
2	9.144925711620587	2.867513914050365
3	3.1251781906660248	1.4699122051206073
4	1.4913774367487804	0.9352827318309713
5	0.9031296517796747	0.7079709896874458
6	0.6002215975827667	0.5646225579706046
7	0.41892140180855286	0.4597544531920763
8	0.33267539253309997	0.4172592963718148
9	0.30750644512568637	0.4339024362132452
>10	3.960493744154127	15.438605268919064
>50	1.1285983511448061	12.471195825436258
>100	1.4762872225178472	49.122659185045755
>500	0.017732135739144988	1.6837983365276394
>1k	0.002086133616369999	0.48809382968916054
>5k	6.953778721233328E-4	0.8531012323693727
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9648	0.5219451762809242	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6002	0.3247009689094224	No Hit
CTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCTT	2110	0.11414845791384226	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT	2057	0.11128122176719125	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11982883141192445	0.0
2	0.0	0.0	0.0	0.3144221978176546	0.0
3	0.0	0.0	0.0	0.438416636461506	0.0
4	5.409879521983045E-5	0.0	0.0	0.5791817016235048	0.0
5	5.409879521983045E-5	0.0	0.0	0.9414813332107094	0.0
6	5.409879521983045E-5	0.0	0.0	1.2266901816096556	0.0
7	5.409879521983045E-5	0.0	0.0	1.405919490172954	0.0
8	5.409879521983045E-5	0.0	0.0	1.7701125795928525	0.0
9	5.409879521983045E-5	0.0	0.0	1.9126088062018858	0.0
10	5.409879521983045E-5	0.0	0.0	2.202361953399298	0.0
11	5.409879521983045E-5	0.0	0.0	2.6169210211688587	0.0
12	5.409879521983045E-5	0.0	0.0	2.9780845780564467	0.0
13	5.409879521983045E-5	0.0	0.0	3.1224742624981743	0.0
14	5.409879521983045E-5	0.0	0.0	3.176735354103664	0.0
15	5.409879521983045E-5	0.0	0.0	3.2592360168139054	0.0
16	5.409879521983045E-5	0.0	0.0	3.442198142247372	0.0
17	1.081975904396609E-4	0.0	0.0	3.654806407461306	0.0
18	1.081975904396609E-4	0.0	0.0	3.9332529064577733	0.0
19	1.081975904396609E-4	0.0	0.0	4.085162323435057	0.0
20	1.081975904396609E-4	0.0	0.0	4.2328520343851945	0.0
21	1.081975904396609E-4	0.0	0.0	4.430745427299334	0.0
22	4.327903617586436E-4	0.0	0.0	4.638863492510022	0.0
23	4.8688915697847406E-4	0.0	0.0	4.871271916774414	0.0
24	9.737783139569481E-4	0.0	0.0	5.056884883173652	0.0
25	0.0010278771091767787	0.0	0.0	5.204736890509448	0.0
26	0.0010278771091767787	0.0	0.0	5.349667562903374	0.0
27	0.001081975904396609	0.0	0.0	5.489080158184877	0.0
28	0.0011360746996164396	0.0	0.0	5.638501030582049	0.0
29	0.0011360746996164396	0.0	0.0	5.79133012707807	0.0
30	0.0011360746996164396	0.0	0.0	5.968449582627795	0.0
31	0.0011360746996164396	0.0	0.0	6.143026394802188	0.0
32	0.0011360746996164396	0.0	0.0	6.312680216611576	0.0
33	0.0011360746996164396	0.0	0.0	6.477627443236839	0.0
34	0.0011360746996164396	0.0	0.0	6.666161744577948	0.0
35	0.0011360746996164396	0.0	0.0	6.879797886901058	0.0
36	0.0011360746996164396	0.0	0.0	7.063409197877164	0.0
37	0.00119017349483627	0.0	0.0	7.2425303088500215	0.0
38	0.0012983710852759308	0.0	0.0	7.423382581269915	0.0
39	0.0013524698804957614	0.0	0.0	7.620789085027076	0.0
40	0.0014065686757155917	0.0	0.0	7.844541702056295	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGAT	35	1.0200165E-7	46.000004	27
GGACGTA	70	0.0	46.000004	8
TATCGTG	35	1.0200165E-7	46.000004	1
GGTCGTA	30	1.862023E-6	46.0	22
CTATCGA	25	3.4179688E-5	46.0	32
TCACACG	25	3.4179688E-5	46.0	1
CGGTTAG	30	1.862023E-6	46.0	35
TTATCCG	20	6.312456E-4	46.0	1
ATCCGTT	20	6.312456E-4	46.0	46
ACGATAC	50	1.6370905E-11	46.0	35
CCCGTAC	20	6.312456E-4	46.0	23
TGCGATA	20	6.312456E-4	46.0	25
CGGGCTA	45	3.110472E-10	46.0	6
ATAACCG	20	6.312456E-4	46.0	41
GCGGAAC	30	1.862023E-6	46.0	16
CGCAACA	25	3.4179688E-5	46.0	17
TATCGAA	30	1.862023E-6	46.0	35
TCGAATC	20	6.312456E-4	46.0	33
TACGTCC	45	3.110472E-10	46.0	43
TATCCGA	40	5.6152203E-9	46.0	39
>>END_MODULE