FastQCFastQC Report
Thu 26 May 2016
SRR1544698_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544698_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1296985
Sequences flagged as poor quality0
Sequence length52
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA91690.7069472661595932No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78590.6059437850090788No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29540.22775899489970972No Hit
CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCTT29410.22675667027760538TruSeq Adapter, Index 21 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCT17170.13238395201178116TruSeq Adapter, Index 15 (95% over 22bp)
AAAACTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCT14470.11156644062961407No Hit
TGCATAGAGAGAAAAGGTTGTAACGTAGAGGAAGTGAGACTGGTGAATTGGT13160.10146609251456262No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGTC206.311756E-446.033
TATTTCG405.6115823E-946.031
AACGTGC453.110472E-1046.018
AACCGTA206.311756E-446.043
AAGTACG453.110472E-1046.01
AGGTACG453.110472E-1046.01
ACTATCG301.8615901E-646.020
GTCGCCT206.311756E-446.037
GTACCCG206.311756E-446.01
CTCGTCC253.4174016E-546.037
TTTACGC600.046.043
TCCGATT750.046.018
CGTGCGT206.311756E-446.044
GCGGTAT301.8615901E-646.031
CTATCGC206.311756E-446.027
CTATCGA301.8615901E-646.021
GCAACTA600.046.024
CCGGCCA206.311756E-446.045
GCAACGA206.311756E-446.029
ACCGCAA301.8615901E-646.037
Produced by FastQC (version 0.11.5)