##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544698_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1296985
Sequences flagged as poor quality	0
Sequence length	52
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.74898476081065	33.0	31.0	34.0	31.0	34.0
2	33.13903553240785	34.0	33.0	34.0	31.0	34.0
3	33.158653338319255	34.0	33.0	34.0	31.0	34.0
4	36.46504238676623	37.0	37.0	37.0	35.0	37.0
5	36.4668095621769	37.0	37.0	37.0	35.0	37.0
6	36.537229806050185	37.0	37.0	37.0	35.0	37.0
7	36.639921818679476	37.0	37.0	37.0	35.0	37.0
8	36.64946626213873	37.0	37.0	37.0	35.0	37.0
9	38.37996584386095	39.0	39.0	39.0	37.0	39.0
10	38.28950835977286	39.0	39.0	39.0	37.0	39.0
11	38.03923946691751	39.0	39.0	39.0	35.0	39.0
12	37.01857847238018	39.0	35.0	39.0	35.0	39.0
13	36.875560627146804	39.0	35.0	39.0	35.0	39.0
14	37.90291406608404	40.0	35.0	41.0	35.0	41.0
15	37.94129847299699	40.0	35.0	41.0	35.0	41.0
16	38.024541532862756	40.0	35.0	41.0	35.0	41.0
17	37.98827434395926	40.0	35.0	41.0	35.0	41.0
18	38.03276676291553	39.0	36.0	41.0	35.0	41.0
19	37.9581483209135	39.0	36.0	41.0	35.0	41.0
20	37.78569682764257	39.0	35.0	41.0	35.0	41.0
21	37.690436666576716	39.0	35.0	41.0	35.0	41.0
22	37.60941876737202	39.0	35.0	41.0	35.0	41.0
23	37.57506601849674	39.0	35.0	41.0	35.0	41.0
24	37.52122807896776	38.0	35.0	41.0	35.0	41.0
25	37.51211617713389	38.0	35.0	41.0	35.0	41.0
26	37.4508872500453	38.0	35.0	41.0	35.0	41.0
27	37.44059106311946	38.0	35.0	41.0	35.0	41.0
28	37.45613634698936	38.0	35.0	41.0	35.0	41.0
29	37.43761878510546	38.0	35.0	41.0	35.0	41.0
30	37.40331306838552	38.0	35.0	41.0	35.0	41.0
31	37.219833691214625	38.0	35.0	41.0	35.0	41.0
32	37.045924201128	38.0	35.0	41.0	34.0	41.0
33	36.88928553529917	38.0	35.0	41.0	34.0	41.0
34	36.727500318045315	38.0	35.0	41.0	34.0	41.0
35	36.5894678812785	38.0	35.0	41.0	34.0	41.0
36	36.50206286117418	38.0	35.0	41.0	33.0	41.0
37	36.42952000215885	37.0	35.0	41.0	33.0	41.0
38	36.31975389075433	37.0	35.0	41.0	33.0	41.0
39	36.3141208263781	37.0	35.0	41.0	33.0	41.0
40	36.15965334988454	37.0	35.0	41.0	32.0	41.0
41	36.18227041947286	37.0	35.0	41.0	33.0	41.0
42	36.18564285631677	37.0	35.0	41.0	33.0	41.0
43	36.11210769592555	37.0	35.0	41.0	33.0	41.0
44	36.00942724858036	36.0	35.0	41.0	33.0	41.0
45	35.921677583009824	36.0	35.0	41.0	33.0	41.0
46	35.87616587701477	36.0	35.0	40.0	33.0	41.0
47	35.8327181887223	36.0	35.0	40.0	33.0	41.0
48	35.81010497422869	36.0	35.0	40.0	33.0	41.0
49	35.836182376820084	36.0	35.0	40.0	32.0	41.0
50	35.69892481408806	36.0	35.0	40.0	32.0	41.0
51	35.61656842600338	36.0	35.0	40.0	32.0	41.0
52	35.379979722201874	36.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	1.0
13	9.0
14	13.0
15	11.0
16	31.0
17	95.0
18	195.0
19	418.0
20	715.0
21	1110.0
22	1880.0
23	3319.0
24	5964.0
25	9294.0
26	11913.0
27	12730.0
28	12168.0
29	11746.0
30	12654.0
31	14779.0
32	18518.0
33	26481.0
34	74588.0
35	375303.0
36	49855.0
37	52454.0
38	101309.0
39	497217.0
40	2212.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.520025289421234	24.995046203309983	29.42948453528761	14.055443971981171
2	29.947300855445512	26.809947686364914	29.734499627983364	13.50825183020621
3	27.712271151940847	26.503082148212968	31.913784662120225	13.870862037725956
4	23.56164489180677	30.935978442310436	31.18301291071215	14.319363755170647
5	21.35005416408054	33.88928939039387	30.378146239162362	14.38251020636322
6	21.256452464754798	42.308430706600305	26.294829932497294	10.140286896147604
7	86.31927123289783	5.5649834038173145	6.1656842600338475	1.9500611032510013
8	88.02831181547974	3.955789773975798	6.177403747923068	1.8384946626213872
9	84.25802919848726	5.899220114342109	7.516740748736493	2.326009938434138
10	54.60124827966399	24.5456192631372	11.720798621418135	9.132333835780676
11	50.11283862188075	19.037228649521776	19.36090240056747	11.489030328030008
12	44.67908264166509	21.55314055289768	21.558460583584235	12.209316221852989
13	18.044233356592404	47.653133999236694	23.89989090081998	10.402741743350925
14	12.519188733871248	48.668411739534385	27.46130448694472	11.351095039649648
15	10.632813795070877	23.312837079842865	54.20317120090055	11.851177924185707
16	13.846960450583467	18.541617674838182	51.78116940442642	15.83025247015193
17	13.360833008862864	18.634525457117853	29.51059572778405	38.49404580623523
18	17.27753212257659	23.446840171628818	41.07040559451343	18.205222111281163
19	27.215580750741143	23.239898688111275	29.425012625435144	20.11950793571244
20	30.842145437302666	22.879755741199784	28.0470475757237	18.231051245773852
21	18.098358886186038	30.343912998222798	32.15588460930543	19.401843506285733
22	19.771315782372195	23.882928484138212	26.82413443486239	29.5216212986272
23	14.83448150903827	32.230904752175235	26.420737325412397	26.513876413374092
24	14.782669036264876	24.211305450718395	44.92488347976268	16.081142033254046
25	12.556120541101093	25.67415968573268	41.805340848197936	19.964378924968294
26	12.383643604205137	38.93260137935288	29.69240199385498	18.991353022587
27	13.225596286772785	43.02863949852928	29.162634880125832	14.583129334572103
28	11.932443320470169	32.7108640423752	39.713720667548195	15.642971969606434
29	12.42180904173911	26.094287906182416	40.059753967856224	21.424149084222254
30	16.414839030520785	36.19610095722001	31.79242628095159	15.59663373130761
31	29.07211725656041	29.580758451331356	26.821744276148145	14.525380015960094
32	30.93482191390031	25.655038416018687	29.994949825942474	13.415189844138522
33	31.309382914991307	28.805344703292633	22.655080822060395	17.230191559655665
34	19.78396049298951	29.09625014938492	26.466150340983123	24.653639016642444
35	16.856709985080784	29.30720093139088	32.79937701669641	21.03671206683192
36	33.67617975535569	25.06675096473745	26.04378616560716	15.213283114299703
37	17.87615122765491	37.22988315207963	29.496023469816535	15.397942150448927
38	18.658272840472325	38.106762992632916	23.071199744021712	20.163764422873047
39	19.64725883491328	35.33032378940389	27.904948785066907	17.11746859061593
40	24.741535175811595	25.89058470221321	25.719110089939363	23.648770032035838
41	14.918368369719001	24.96235500025058	29.16186386118575	30.957412768844666
42	20.12266911336677	23.409831262504962	27.215503648847132	29.25199597528113
43	19.941633866235925	24.176995107884824	28.79855973661993	27.082811289259322
44	16.328947520595843	30.14159762834574	31.67330385470919	21.856150996349225
45	15.53996383921171	42.685073458829514	23.634814589220383	18.140148112738387
46	21.59115178664364	34.29762102106038	26.850194875037104	17.261032317258874
47	18.915484758883103	29.108663554320213	26.980882585380712	24.994969101415975
48	20.987289752772778	25.383254239640397	34.11296198491116	19.51649402267567
49	22.45677475067175	23.778147010181307	35.14720679113482	18.61787144801212
50	19.74764550091173	35.65183868741736	26.951815171339682	17.64870064033123
51	17.152781258071602	35.03494643345913	25.36351615477434	22.448756153694916
52	18.806385578861747	27.461073181262698	36.579220268545896	17.15332097132966
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	10371.0
1	8415.0
2	6459.0
3	9809.0
4	13159.0
5	8730.0
6	4301.0
7	4976.5
8	5652.0
9	5906.5
10	6161.0
11	6272.0
12	6383.0
13	6185.5
14	5779.0
15	5570.0
16	5579.5
17	5589.0
18	5202.0
19	4815.0
20	5349.5
21	5884.0
22	5568.5
23	5253.0
24	5648.0
25	6043.0
26	7803.0
27	9563.0
28	11413.0
29	13263.0
30	16595.5
31	19928.0
32	22165.5
33	24403.0
34	28163.0
35	31923.0
36	37151.0
37	42379.0
38	44268.5
39	70545.0
40	94932.0
41	124856.0
42	154780.0
43	168584.5
44	182389.0
45	169957.0
46	157525.0
47	142524.0
48	127523.0
49	109798.0
50	92073.0
51	78982.5
52	65892.0
53	54627.5
54	43363.0
55	37015.5
56	30668.0
57	25809.5
58	20951.0
59	18232.0
60	15513.0
61	12874.5
62	10236.0
63	8542.5
64	5890.5
65	4932.0
66	4048.0
67	3164.0
68	2797.0
69	2430.0
70	1936.5
71	1443.0
72	1349.0
73	1255.0
74	1041.5
75	828.0
76	647.5
77	467.0
78	354.0
79	241.0
80	208.0
81	175.0
82	115.5
83	56.0
84	50.5
85	45.0
86	24.5
87	4.0
88	4.0
89	2.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1296985.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.98595329600461
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.49398954759351	12.068433910368903
2	10.60593646320588	3.39092009922403
3	3.8767117416202583	1.859187985308424
4	1.857649376751221	1.1878518470838837
5	1.0432988068516142	0.8339063000053619
6	0.6947802238592702	0.6664034525761152
7	0.48917233528778187	0.5473920273925592
8	0.387439571559932	0.49548727167848866
9	0.2990094377200843	0.43019558158120796
>10	2.826233087269351	10.391053310036053
>50	1.0079523316159003	11.550549723855747
>100	1.391633897691562	52.090296326110476
>500	0.021827649144691635	2.192557357622864
>1k	0.003395412089174254	0.9753918164357762
>5k	9.701177397640726E-4	1.3203729907201207
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9169	0.7069472661595932	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7859	0.6059437850090788	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2954	0.22775899489970972	No Hit
CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCTT	2941	0.22675667027760538	TruSeq Adapter, Index 21 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCT	1717	0.13238395201178116	TruSeq Adapter, Index 15 (95% over 22bp)
AAAACTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCT	1447	0.11156644062961407	No Hit
TGCATAGAGAGAAAAGGTTGTAACGTAGAGGAAGTGAGACTGGTGAATTGGT	1316	0.10146609251456262	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.71018940080263E-5	0.0	0.0	0.23616310134658458	0.0
2	7.71018940080263E-5	0.0	0.0	0.5725586649036034	0.0
3	1.542037880160526E-4	0.0	0.0	0.7674722529558938	0.0
4	1.542037880160526E-4	0.0	0.0	1.0013222974822376	0.0
5	1.542037880160526E-4	0.0	0.0	1.6914613507480811	0.0
6	1.542037880160526E-4	0.0	0.0	2.206887512191737	0.0
7	1.542037880160526E-4	0.0	0.0	2.5273229836890945	0.0
8	1.542037880160526E-4	0.0	0.0	3.1816867581352137	0.0
9	1.542037880160526E-4	0.0	0.0	3.426562373504705	0.0
10	2.3130568202407893E-4	0.0	0.0	3.9029749765802997	0.0
11	2.3130568202407893E-4	0.0	0.0	4.530815699487658	0.0
12	2.3130568202407893E-4	0.0	0.0	5.03976530183464	0.0
13	2.3130568202407893E-4	0.0	0.0	5.241232550877612	0.0
14	2.3130568202407893E-4	0.0	0.0	5.306614956996419	0.0
15	2.3130568202407893E-4	0.0	0.0	5.430594802561325	0.0
16	2.3130568202407893E-4	0.0	0.0	5.70191636757557	0.0
17	2.3130568202407893E-4	0.0	0.0	6.011943083381843	0.0
18	2.3130568202407893E-4	0.0	0.0	6.466998461817215	0.0
19	2.3130568202407893E-4	0.0	0.0	6.662066253657521	0.0
20	3.084075760321052E-4	0.0	0.0	6.882269262944444	0.0
21	3.084075760321052E-4	0.0	0.0	7.1700906332764065	0.0
22	3.084075760321052E-4	0.0	0.0	7.424912392972933	0.0
23	3.084075760321052E-4	0.0	0.0	7.739565222419689	0.0
24	3.084075760321052E-4	0.0	0.0	7.957994888144428	0.0
25	3.084075760321052E-4	0.0	0.0	8.14149739588353	0.0
26	3.084075760321052E-4	0.0	0.0	8.327544266124898	0.0
27	3.084075760321052E-4	0.0	0.0	8.505880946965462	0.0
28	3.084075760321052E-4	0.0	0.0	8.712514022906973	0.0
29	3.084075760321052E-4	0.0	0.0	8.908044426111328	0.0
30	3.084075760321052E-4	0.0	0.0	9.156466728605189	0.0
31	3.084075760321052E-4	0.0	0.0	9.394326071619949	0.0
32	3.084075760321052E-4	0.0	0.0	9.604814242261861	0.0
33	3.084075760321052E-4	0.0	0.0	9.82756161405105	0.0
34	3.084075760321052E-4	0.0	0.0	10.042598796439435	0.0
35	3.084075760321052E-4	0.0	0.0	10.33111408381747	0.0
36	3.084075760321052E-4	0.0	0.0	10.545997062417838	0.0
37	3.084075760321052E-4	0.0	0.0	10.76859023041901	0.0
38	3.084075760321052E-4	0.0	0.0	10.97522330636052	0.0
39	3.084075760321052E-4	0.0	0.0	11.187947431928665	0.0
40	3.084075760321052E-4	0.0	0.0	11.438528587454751	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGTC	20	6.311756E-4	46.0	33
TATTTCG	40	5.6115823E-9	46.0	31
AACGTGC	45	3.110472E-10	46.0	18
AACCGTA	20	6.311756E-4	46.0	43
AAGTACG	45	3.110472E-10	46.0	1
AGGTACG	45	3.110472E-10	46.0	1
ACTATCG	30	1.8615901E-6	46.0	20
GTCGCCT	20	6.311756E-4	46.0	37
GTACCCG	20	6.311756E-4	46.0	1
CTCGTCC	25	3.4174016E-5	46.0	37
TTTACGC	60	0.0	46.0	43
TCCGATT	75	0.0	46.0	18
CGTGCGT	20	6.311756E-4	46.0	44
GCGGTAT	30	1.8615901E-6	46.0	31
CTATCGC	20	6.311756E-4	46.0	27
CTATCGA	30	1.8615901E-6	46.0	21
GCAACTA	60	0.0	46.0	24
CCGGCCA	20	6.311756E-4	46.0	45
GCAACGA	20	6.311756E-4	46.0	29
ACCGCAA	30	1.8615901E-6	46.0	37
>>END_MODULE