FastQCFastQC Report
Thu 26 May 2016
SRR1544696_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544696_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1194997
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA88430.7400018577452496No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50740.42460357641065205No Hit
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCTT40700.34058662908777176TruSeq Adapter, Index 14 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT30660.2565696817648914TruSeq Adapter, Index 15 (95% over 22bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26060.21807586127831283No Hit
AAAACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT21860.18292932952969757No Hit
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT14050.11757351692096299TruSeq Adapter, Index 15 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGAA453.092282E-1046.00000446
GATACGT453.092282E-1046.00000411
CCTTACA351.0196163E-746.00000423
GTCGAAT700.046.00000412
ATTACCG351.0196163E-746.00000425
GGATCCG453.092282E-1046.0000041
GTTAGTC351.0196163E-746.00000436
TAAGTCC351.0196163E-746.00000428
TGCGGTC453.092282E-1046.0000044
CACGACA351.0196163E-746.00000439
CGACAAC351.0196163E-746.00000426
GCTTGAT453.092282E-1046.00000436
TGTGATA453.092282E-1046.00000419
CGTTAAG351.0196163E-746.00000425
GTAGCAC351.0196163E-746.00000435
TCCAGCG351.0196163E-746.0000041
CGGATCT351.0196163E-746.00000427
TACGACA351.0196163E-746.00000445
ACTACGA351.0196163E-746.00000443
TCGTCAA351.0196163E-746.00000440