##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544696_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1194997
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.70739926543749	33.0	31.0	34.0	31.0	34.0
2	33.09665045184214	34.0	33.0	34.0	31.0	34.0
3	33.12261537058252	34.0	33.0	34.0	31.0	34.0
4	36.45083293096133	37.0	37.0	37.0	35.0	37.0
5	36.431202756157546	37.0	37.0	37.0	35.0	37.0
6	36.50368076237848	37.0	37.0	37.0	35.0	37.0
7	36.6143772745873	37.0	37.0	37.0	35.0	37.0
8	36.61220572101855	37.0	37.0	37.0	35.0	37.0
9	38.35233812302457	39.0	39.0	39.0	37.0	39.0
10	38.25208264121165	39.0	39.0	39.0	37.0	39.0
11	37.926383915608156	39.0	38.0	39.0	35.0	39.0
12	36.671009215922716	37.0	35.0	39.0	35.0	39.0
13	36.47463466435481	37.0	35.0	39.0	35.0	39.0
14	37.351782473094076	38.0	35.0	41.0	35.0	41.0
15	37.44974589894368	38.0	35.0	41.0	35.0	41.0
16	37.566417321549764	38.0	35.0	41.0	35.0	41.0
17	37.54900639917925	38.0	35.0	41.0	35.0	41.0
18	37.6153538460766	37.0	35.0	41.0	35.0	41.0
19	37.531813050576694	37.0	35.0	41.0	35.0	41.0
20	37.316833431381	36.0	35.0	41.0	35.0	41.0
21	37.204240680102124	36.0	35.0	41.0	35.0	41.0
22	37.12007812571915	35.0	35.0	41.0	35.0	41.0
23	37.07966379831916	35.0	35.0	41.0	35.0	41.0
24	37.01909460860571	35.0	35.0	41.0	35.0	41.0
25	36.99146022960727	35.0	35.0	41.0	35.0	41.0
26	36.905116916611505	35.0	35.0	41.0	35.0	41.0
27	36.88906164617986	35.0	35.0	41.0	34.0	41.0
28	36.94262496056476	36.0	35.0	41.0	35.0	41.0
29	36.93453791097384	36.0	35.0	41.0	35.0	41.0
30	36.896361246095175	36.0	35.0	41.0	35.0	41.0
31	36.686802561010616	36.0	35.0	41.0	34.0	41.0
32	36.5040899684267	35.0	35.0	41.0	34.0	41.0
33	36.35912809822953	35.0	35.0	41.0	34.0	41.0
34	36.20685993353958	35.0	35.0	41.0	34.0	41.0
35	36.056643656846	35.0	35.0	41.0	33.0	41.0
36	35.94441576003957	35.0	35.0	40.0	33.0	41.0
37	35.897475056422735	35.0	35.0	40.0	33.0	41.0
38	35.81749493931784	35.0	35.0	40.0	33.0	41.0
39	35.85165569453312	35.0	35.0	40.0	33.0	41.0
40	35.668825110021196	35.0	35.0	40.0	31.0	41.0
41	35.68506531815561	35.0	35.0	40.0	32.0	41.0
42	35.66868619753857	35.0	35.0	40.0	33.0	41.0
43	35.60415967571467	35.0	35.0	40.0	33.0	41.0
44	35.516099203596326	35.0	35.0	40.0	32.0	41.0
45	35.45008230146185	35.0	35.0	40.0	32.0	41.0
46	35.41517091674707	35.0	35.0	40.0	32.0	41.0
47	35.34087616956361	35.0	35.0	40.0	32.0	41.0
48	35.299657656044324	35.0	35.0	40.0	32.0	41.0
49	35.36120090678052	36.0	35.0	40.0	32.0	41.0
50	35.18180966144685	35.0	35.0	40.0	31.0	41.0
51	35.08989478634675	35.0	35.0	40.0	31.0	41.0
52	34.858138555996376	35.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	0.0
13	0.0
14	8.0
15	8.0
16	42.0
17	86.0
18	207.0
19	436.0
20	870.0
21	1281.0
22	2233.0
23	3688.0
24	5916.0
25	8711.0
26	11189.0
27	12108.0
28	11602.0
29	11840.0
30	12841.0
31	15044.0
32	19418.0
33	28017.0
34	79402.0
35	419012.0
36	46459.0
37	45811.0
38	85522.0
39	371508.0
40	1735.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.901249124474788	25.576884293433373	29.165679913840787	14.35618666825105
2	29.029361579987228	27.471449719120635	30.409281362212624	13.08990733867951
3	26.790611189818886	27.227850781215352	32.24326086174275	13.738277167223014
4	25.050272092733287	30.052293018308834	31.262505261519486	13.634929627438394
5	21.27243834084939	35.636407455416204	28.836055655369847	14.255098548364556
6	21.349677028477895	42.14353676201698	26.103831223007255	10.402954986497875
7	86.34573978009986	5.765202757831191	5.814073173405456	2.074984288663486
8	87.16783389414367	4.357082067988456	5.912148733427783	2.562935304440095
9	84.26029521413024	5.226372953237539	7.667801676489565	2.8455301561426514
10	61.91153617958874	19.429170115071418	10.950404059591781	7.708889645748064
11	57.04750723223573	16.915272590642488	16.058785084816112	9.97843509230567
12	50.090753365908036	20.767750881382966	18.641971486120887	10.499524266588118
13	18.43142702450299	52.45494340153155	19.68138832147696	9.4322412524885
14	10.632746358359059	54.50030418486407	24.183491674037676	10.683457782739204
15	9.12537855743571	23.335790801148455	57.71035408457092	9.828476556844914
16	10.452243813164385	18.1702548207234	55.615871839008804	15.761629527103416
17	11.184044813501623	18.015442716592595	27.022913028233543	43.77759944167224
18	16.565731964180664	22.811438020346493	41.00780169322601	19.615028322246832
19	30.704763275556342	20.838880767064687	27.30718152430508	21.149174433073892
20	33.04886957875208	22.248424054621058	26.530192126005335	18.172514240621524
21	17.682638533820587	33.484770254653355	30.584930338737255	18.247660872788803
22	20.028083752511513	25.168096656309597	22.589429094801076	32.21439049637782
23	13.866645690323908	33.41263618235025	23.05930475139268	29.66141337593316
24	14.433509038098002	22.0800554311015	47.639282776442116	15.84715275435838
25	11.645301201592975	24.914121123316626	44.16429497312546	19.27628270196494
26	11.003207539433154	40.603198167024686	28.075635336323018	20.31795895721914
27	12.472834659835966	47.03526452367663	25.26023077882204	15.231670037665365
28	10.047807651399962	33.80677943124543	41.34964355559052	14.795769361764089
29	10.281448405309805	25.487009590819053	40.25859479145136	23.97294721241978
30	14.76346802544274	38.309803288209096	29.929196474970233	16.997532211377937
31	30.386436116576025	29.86610008225962	24.03688042731488	15.710583373849474
32	33.154141809561025	24.898974641777343	29.253713607649225	12.693169941012405
33	32.21514363634385	27.670362352374106	22.11294254295199	18.001551468330046
34	18.76916845816349	28.481075684708834	25.215460791951777	27.534295065175897
35	17.169164441417006	25.320230929450034	33.93029438567628	23.58031024345668
36	36.37858505084113	21.690514704221016	26.058642825044746	15.872257419893105
37	17.514939368048623	36.94636890301817	30.574972154741808	14.9637195741914
38	17.047657860228938	38.658507092486424	22.734534061591784	21.55930098569285
39	19.735614399031963	35.018330589951276	28.45228900156235	16.793766009454416
40	27.470194485843898	23.960896972963113	24.186420551683394	24.382487989509595
41	14.356939808217092	23.141145961035885	28.581326982410832	33.92058724833619
42	19.640300352218457	20.916035772474743	27.501575317762306	31.942088557544494
43	20.391264580580536	20.650679457772696	29.567187197959495	29.390868763687273
44	14.168152723395957	29.86969841765293	31.912465052213516	24.04968380673759
45	13.431247107733324	45.84881803050552	22.145494925928684	18.574439935832473
46	21.92582910249984	36.49055185912601	24.71847209658267	16.865146941791487
47	18.734272973070226	28.405259594793964	25.63186351095442	27.228603921181392
48	20.23862821412941	24.73579431580163	36.15950500294143	18.86607246712753
49	22.873613908654164	21.79051495526767	36.433815315017526	18.90205582106064
50	20.155029677898774	36.93975800776069	25.957722069595153	16.947490244745385
51	16.132007025959062	36.30477733416904	23.606921188923486	23.956294450948413
52	17.948496941833326	27.353625155544325	36.98695477896597	17.71092312365638
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9969.0
1	7766.5
2	5564.0
3	7448.0
4	9332.0
5	6485.0
6	3638.0
7	3973.5
8	4309.0
9	4621.5
10	4934.0
11	5353.5
12	5773.0
13	5903.5
14	5892.5
15	5751.0
16	5528.0
17	5305.0
18	4940.0
19	4575.0
20	4283.0
21	3991.0
22	3612.0
23	3233.0
24	4050.5
25	4868.0
26	6333.0
27	7798.0
28	9260.0
29	10722.0
30	11648.5
31	12575.0
32	12955.5
33	13336.0
34	17783.5
35	22231.0
36	22989.5
37	23748.0
38	32241.0
39	62675.0
40	84616.0
41	120371.0
42	156126.0
43	165742.5
44	175359.0
45	162034.5
46	148710.0
47	133845.0
48	118980.0
49	105628.5
50	92277.0
51	81335.5
52	70394.0
53	59351.5
54	48309.0
55	38904.0
56	29499.0
57	24629.5
58	19760.0
59	16739.0
60	13718.0
61	11799.5
62	9881.0
63	8249.0
64	5737.0
65	4857.0
66	3731.5
67	2606.0
68	2265.5
69	1925.0
70	1554.5
71	1184.0
72	966.5
73	749.0
74	686.5
75	624.0
76	406.5
77	189.0
78	123.0
79	57.0
80	59.0
81	61.0
82	61.0
83	61.0
84	37.0
85	13.0
86	7.5
87	2.0
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1194997.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.691639349095507
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.79327425449523	11.736290853395294
2	10.93189250263137	3.430786291087452
3	4.177644608381731	1.9666227757025836
4	1.999959543085508	1.2553057545151851
5	1.1101719154011944	0.8710208655985057
6	0.767485582398935	0.7225864178780768
7	0.5025668734146437	0.5520268688497587
8	0.35682931465395806	0.4479389531787864
9	0.28736322803031417	0.4058280122799225
>10	2.65453024201379	9.666786635015947
>50	0.9785730670233553	10.946434081806773
>100	1.4043193767310376	52.31821493045922
>500	0.02895503869548531	2.933683711099656
>1k	0.0053620442028676505	1.5755077147543823
>5k	0.00107240884057353	1.170966134378464
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8843	0.7400018577452496	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5074	0.42460357641065205	No Hit
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCTT	4070	0.34058662908777176	TruSeq Adapter, Index 14 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT	3066	0.2565696817648914	TruSeq Adapter, Index 15 (95% over 22bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2606	0.21807586127831283	No Hit
AAAACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT	2186	0.18292932952969757	No Hit
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT	1405	0.11757351692096299	TruSeq Adapter, Index 15 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.35339000851048163	0.0
2	0.0	0.0	0.0	0.93180150243055	0.0
3	0.0	0.0	0.0	1.2275344624296127	0.0
4	0.0	0.0	0.0	1.5533930210703457	0.0
5	0.0	0.0	0.0	2.4967426696468693	0.0
6	0.0	0.0	0.0	3.170049799288199	0.0
7	0.0	0.0	0.0	3.610134586111932	0.0
8	0.0	0.0	0.0	4.42511571158756	0.0
9	0.0	0.0	0.0	4.716831925101067	0.0
10	0.0	0.0	0.0	5.216080040368302	0.0
11	0.0	0.0	0.0	5.931981419200215	0.0
12	0.0	0.0	0.0	6.505120933357992	0.0
13	0.0	0.0	0.0	6.728552456617046	0.0
14	0.0	0.0	0.0	6.805205368716407	0.0
15	0.0	0.0	0.0	6.939347964890288	0.0
16	0.0	0.0	0.0	7.2420265490206255	0.0
17	0.0	0.0	0.0	7.5861278312832585	0.0
18	0.0	0.0	0.0	8.156756878887562	0.0
19	0.0	0.0	0.0	8.379602626617473	0.0
20	0.0	0.0	0.0	8.60454042980861	0.0
21	0.0	0.0	0.0	8.928306932988116	0.0
22	3.347288737963359E-4	0.0	0.0	9.197177900865023	0.0
23	6.694577475926718E-4	0.0	0.0	9.556593029103839	0.0
24	8.368221844908397E-4	0.0	0.0	9.79500366946528	0.0
25	0.0010041866213890078	0.0	0.0	9.97943927892706	0.0
26	0.0013389154951853437	0.0	0.0	10.1950046736519	0.0
27	0.0013389154951853437	0.0	0.0	10.3593565506859	0.0
28	0.0013389154951853437	0.0	0.0	10.53567498495812	0.0
29	0.0013389154951853437	0.0	0.0	10.722704743191825	0.0
30	0.0013389154951853437	0.0	0.0	10.99986025069519	0.0
31	0.0013389154951853437	0.0	0.0	11.275509478266473	0.0
32	0.0013389154951853437	0.0	0.0	11.491911695175803	0.0
33	0.0014225977136344274	0.0	0.0	11.68680758194372	0.0
34	0.0014225977136344274	0.0	0.0	11.903711892163747	0.0
35	0.0014225977136344274	0.0	0.0	12.208649896192208	0.0
36	0.0014225977136344274	0.0	0.0	12.446140032150709	0.0
37	0.0014225977136344274	0.0	0.0	12.667730546603883	0.0
38	0.0014225977136344274	0.0	0.0	12.868400506444786	0.0
39	0.0014225977136344274	0.0	0.0	13.056852862392123	0.0
40	0.0014225977136344274	0.0	0.0	13.287983149748493	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGAA	45	3.092282E-10	46.000004	46
GATACGT	45	3.092282E-10	46.000004	11
CCTTACA	35	1.0196163E-7	46.000004	23
GTCGAAT	70	0.0	46.000004	12
ATTACCG	35	1.0196163E-7	46.000004	25
GGATCCG	45	3.092282E-10	46.000004	1
GTTAGTC	35	1.0196163E-7	46.000004	36
TAAGTCC	35	1.0196163E-7	46.000004	28
TGCGGTC	45	3.092282E-10	46.000004	4
CACGACA	35	1.0196163E-7	46.000004	39
CGACAAC	35	1.0196163E-7	46.000004	26
GCTTGAT	45	3.092282E-10	46.000004	36
TGTGATA	45	3.092282E-10	46.000004	19
CGTTAAG	35	1.0196163E-7	46.000004	25
GTAGCAC	35	1.0196163E-7	46.000004	35
TCCAGCG	35	1.0196163E-7	46.000004	1
CGGATCT	35	1.0196163E-7	46.000004	27
TACGACA	35	1.0196163E-7	46.000004	45
ACTACGA	35	1.0196163E-7	46.000004	43
TCGTCAA	35	1.0196163E-7	46.000004	40
>>END_MODULE