FastQCFastQC Report
Thu 26 May 2016
SRR1544695_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544695_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1585879
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83210.5246932458277082No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35040.22095002203825134No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATA206.312183E-446.026
CGTATCA301.8618539E-646.029
ACGTTCC206.312183E-446.041
ATCGTAC206.312183E-446.034
ACGATTG301.8618539E-646.01
CCGTAAG206.312183E-446.01
GGACGTA501.6370905E-1146.08
GCGATAC453.110472E-1046.09
ACGTATT301.8618539E-646.035
CGGTCAT405.6134013E-946.034
TATCGGC206.312183E-446.046
ATCGATT253.4177465E-546.017
TCGCATG351.01990736E-745.9999961
CTAACCG351.01990736E-745.99999645
TTAACGG1200.044.0833322
ATATGCG1100.043.9090921
ACGGGTA1500.042.9333345
CGTTAGG2750.041.818182
TCGTTTA508.731149E-1041.438
AACGGGA5100.041.0392154
Produced by FastQC (version 0.11.5)