FastQCFastQC Report
Thu 26 May 2016
SRR1544694_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544694_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1675842
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87400.5215288792141503No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA77760.464005556609752No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21120.1260262005606734No Hit
CTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGCTT18270.10901982406455978Illumina Single End Adapter 1 (95% over 23bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG551.8189894E-1246.0000041
ACGATAT351.01994374E-746.0000041
TACGTCC700.046.00000425
CGGAATA206.3122873E-446.033
CGTATTC206.3122873E-446.022
AGTCACG253.4178305E-546.01
GTCGAAC253.4178305E-546.012
ACGCATC206.3122873E-446.039
CTATCGT301.8619176E-646.035
TTAGCGA301.8619176E-646.041
GACAATC206.3122873E-446.021
TAAACGG206.3122873E-446.02
CAATTCG301.8619176E-646.01
CGACATA206.3122873E-446.043
TATGCGC301.8619176E-646.011
GCGTTGC206.3122873E-446.029
CGTAGTC301.8619176E-646.018
GCGATCG253.4178305E-546.09
TTAACGC253.4178305E-546.046
ACGCTAA253.4178305E-546.011
Produced by FastQC (version 0.11.5)