##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544694_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1675842
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.6916517189568	33.0	31.0	34.0	31.0	34.0
2	33.10690267936953	34.0	33.0	34.0	31.0	34.0
3	33.14562709372363	34.0	33.0	34.0	31.0	34.0
4	36.47677406342603	37.0	37.0	37.0	35.0	37.0
5	36.470667879191474	37.0	37.0	37.0	35.0	37.0
6	36.53180669776745	37.0	37.0	37.0	35.0	37.0
7	36.61888829615202	37.0	37.0	37.0	35.0	37.0
8	36.615934557076386	37.0	37.0	37.0	35.0	37.0
9	38.341442689704635	39.0	39.0	39.0	37.0	39.0
10	38.25100635978809	39.0	39.0	39.0	37.0	39.0
11	38.06846946191825	39.0	39.0	39.0	35.0	39.0
12	37.28077885624062	39.0	35.0	39.0	35.0	39.0
13	37.13079574327413	39.0	35.0	39.0	35.0	39.0
14	38.27156140017973	40.0	35.0	41.0	35.0	41.0
15	38.29894405319833	40.0	35.0	41.0	35.0	41.0
16	38.38130503949656	40.0	35.0	41.0	35.0	41.0
17	38.32890332143484	40.0	35.0	41.0	35.0	41.0
18	38.347704616544995	40.0	36.0	41.0	35.0	41.0
19	38.30626037538145	40.0	36.0	41.0	35.0	41.0
20	38.14506439151185	40.0	35.0	41.0	35.0	41.0
21	38.04383289116755	40.0	35.0	41.0	35.0	41.0
22	37.9556461766682	40.0	35.0	41.0	35.0	41.0
23	37.88131279679111	40.0	35.0	41.0	35.0	41.0
24	37.847460560124404	40.0	35.0	41.0	35.0	41.0
25	37.8286717960285	40.0	35.0	41.0	35.0	41.0
26	37.771148473424105	40.0	35.0	41.0	35.0	41.0
27	37.753260748925015	40.0	35.0	41.0	35.0	41.0
28	37.74196672478671	40.0	35.0	41.0	35.0	41.0
29	37.69946570142054	39.0	35.0	41.0	35.0	41.0
30	37.64908983066423	39.0	35.0	41.0	35.0	41.0
31	37.50989413083095	39.0	35.0	41.0	35.0	41.0
32	37.36188793454276	39.0	35.0	41.0	34.0	41.0
33	37.199147055629346	39.0	35.0	41.0	34.0	41.0
34	37.05473726043386	39.0	35.0	41.0	34.0	41.0
35	36.91783592964015	39.0	35.0	41.0	34.0	41.0
36	36.82024260043608	39.0	35.0	41.0	33.0	41.0
37	36.74583701804824	39.0	35.0	41.0	33.0	41.0
38	36.62235222652255	39.0	35.0	41.0	33.0	41.0
39	36.63021812318822	39.0	35.0	41.0	33.0	41.0
40	36.49125812576603	39.0	35.0	41.0	32.0	41.0
41	36.475865266534676	38.0	35.0	41.0	33.0	41.0
42	36.46663169916973	38.0	35.0	41.0	33.0	41.0
43	36.409208624679415	38.0	35.0	41.0	33.0	41.0
44	36.29277044017276	38.0	35.0	41.0	33.0	41.0
45	36.218690067440725	38.0	35.0	41.0	32.0	41.0
46	36.18453350614199	38.0	35.0	41.0	32.0	41.0
47	36.13587259419444	38.0	35.0	41.0	32.0	41.0
48	36.089910027317615	37.0	35.0	41.0	32.0	41.0
49	36.05777871660932	37.0	35.0	41.0	32.0	41.0
50	35.924253002371344	37.0	35.0	40.0	32.0	41.0
51	35.853529748031136	37.0	35.0	40.0	32.0	41.0
52	35.61760476226279	37.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	6.0
11	10.0
12	12.0
13	16.0
14	22.0
15	52.0
16	80.0
17	159.0
18	350.0
19	607.0
20	985.0
21	1563.0
22	2578.0
23	4337.0
24	7270.0
25	11191.0
26	14995.0
27	16657.0
28	16034.0
29	15657.0
30	16652.0
31	19237.0
32	23928.0
33	32658.0
34	88599.0
35	384790.0
36	66753.0
37	82648.0
38	157721.0
39	707368.0
40	2907.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.853002848717242	24.958916174675178	28.589031662889465	14.599049313718119
2	29.851799871348256	25.60360702261908	30.998148990179264	13.546444115853404
3	27.88711584982355	25.885256485993306	32.41373590111717	13.81389176306597
4	24.42419989473948	28.518022582081127	31.858492626393183	15.199284896786214
5	23.467427120217778	32.94123192997908	29.372279725654327	14.219061224148815
6	21.27557371160288	41.65195764278494	26.385721326950872	10.686747318661306
7	87.24545631390072	4.656703913614768	6.423576924316254	1.674262848168264
8	87.88513475614049	3.966066013383123	6.328042858455631	1.8207563720207514
9	83.67996505637166	5.869646422514772	7.762545633776931	2.6878428873366342
10	47.805998417511915	29.624630484258063	12.65727914684081	9.912091951389213
11	45.07161176292276	20.428596490599947	21.673642264604897	12.826149481872395
12	41.34363502048522	20.389571331903607	24.520032318082492	13.746761329528677
13	20.42215196898037	40.96889802260595	24.156632904533957	14.452317103879722
14	14.494564523385856	42.75045022144092	26.95057171260775	15.804413542565468
15	13.029092241392684	25.104753312066414	47.72198095047146	14.14417349606944
16	15.517393644508253	20.458670924824656	46.467626423016014	17.55630900765108
17	15.164078713864434	20.35800511026696	30.21710877278407	34.260807403084534
18	18.308706906737033	24.816301298093734	37.83560741406409	19.03938438110514
19	27.35723296110254	23.93662409702108	28.61904642561769	20.087096516258693
20	30.339614355052564	23.79711213825647	28.010934205014554	17.852339301676412
21	20.482539523415692	29.314100016588675	30.26806823077593	19.9352922292197
22	21.0888615991245	24.39794443628934	26.083902897767214	28.429291066818944
23	16.154923912874843	31.186949605034364	26.9424563890868	25.715670093003993
24	16.43496224584418	23.333882311100925	42.913950121789526	17.31720532126537
25	15.443102631393652	26.167502664332314	38.43112894891046	19.958265755363573
26	15.25233285715479	35.781654833808915	28.542487895636942	20.423524413399356
27	15.081553034235926	39.03369172034118	28.481085925761494	17.4036693196614
28	14.228011948620455	31.38028525362176	37.88071906540115	16.510983732356628
29	14.916621017971861	25.287706120266705	36.75710478672811	23.03856807503333
30	17.351516431739984	32.87577229834316	32.01584636260459	17.756864907312263
31	26.944723905952948	29.627793073571375	26.942516060583277	16.4849669598924
32	29.465307588662892	25.379003509877425	29.302702760761456	15.852986140698228
33	28.38244894208404	28.603054464561694	24.473309536340537	18.541187057013726
34	19.051199337407702	28.349271590042495	27.55713247430247	25.04239659824733
35	18.76811775811801	26.33947591718074	32.60384928889478	22.288557035806477
36	30.43437269145898	25.93036813733037	26.494383122036563	17.140876049174086
37	18.50120715437374	35.89765622296135	30.1475318078912	15.453604814773708
38	19.81809741013771	34.27292071686949	26.16732364984288	19.741658223149916
39	21.055326218104092	33.339718183456434	27.83454526142679	17.770410337012677
40	24.55953484875066	26.361017327409147	27.516675199690667	21.56277262414953
41	16.22336711933464	26.274195300034254	29.947751637684224	27.55468594294689
42	20.55862068142462	24.053699573110116	27.364035511700983	28.023644233764283
43	20.11764832245522	24.733954632954656	28.916329821069052	26.23206722352107
44	17.28199913834359	29.538524514840898	31.623625616257378	21.55585073055813
45	16.096386174830325	39.750167378547616	24.822984505699225	19.330461940922834
46	19.787068231969364	34.59926413110544	26.77119919419611	18.84246844272909
47	18.584866592435326	29.123807614321635	28.565461421780814	23.725864371462226
48	20.16622092058798	25.44380675505209	34.81354447495647	19.576427849403462
49	21.93840469447597	25.40394619540506	33.61510213970052	19.042546970418453
50	19.15019435006403	35.19615811037078	27.803575754754924	17.85007178481026
51	17.53303712402482	33.73438546115923	26.31608469056152	22.416492724254432
52	19.053645868763283	27.2699932332523	35.42917530411579	18.247185593868632
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	8688.0
1	8116.0
2	7544.0
3	11906.5
4	16269.0
5	11387.0
6	6505.0
7	6731.0
8	6957.0
9	7428.0
10	7899.0
11	7898.0
12	7897.0
13	7969.5
14	7837.0
15	7632.0
16	6580.5
17	5529.0
18	5636.5
19	5744.0
20	6212.5
21	6681.0
22	6863.5
23	7046.0
24	8455.0
25	9864.0
26	13213.5
27	16563.0
28	19787.0
29	23011.0
30	27048.0
31	31085.0
32	37734.5
33	44384.0
34	51084.5
35	57785.0
36	57595.5
37	57406.0
38	64585.5
39	94053.0
40	116341.0
41	135829.5
42	155318.0
43	171184.0
44	187050.0
45	176854.0
46	166658.0
47	152890.5
48	139123.0
49	131763.0
50	124403.0
51	104732.5
52	85062.0
53	78281.5
54	71501.0
55	60407.5
56	49314.0
57	44878.0
58	40442.0
59	38024.0
60	35606.0
61	31965.5
62	28325.0
63	24277.5
64	16702.0
65	13174.0
66	10962.0
67	8750.0
68	7675.5
69	6601.0
70	5628.5
71	4656.0
72	4155.5
73	3655.0
74	2876.0
75	2097.0
76	1773.0
77	1449.0
78	1219.0
79	989.0
80	701.0
81	413.0
82	305.5
83	198.0
84	158.5
85	119.0
86	87.5
87	56.0
88	34.0
89	7.5
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1675842.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.260749029049181
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.37587018134795	11.808137357418413
2	9.048524114732617	2.7617451119646774
3	3.0697393700314857	1.4053956633192612
4	1.4440515745800875	0.8814923465868004
5	0.8875528866391504	0.6772360926504106
6	0.5963896490149047	0.5460811654283513
7	0.4654091065458368	0.4971744099581018
8	0.3319183526111796	0.405225814186773
9	0.2641323185238159	0.36277713211075496
>10	3.7466976174839877	14.313368753562022
>50	1.171620847804402	12.510584704628592
>100	1.5815701138237819	50.877790656720464
>500	0.012589612846358463	1.2435415027820227
>1k	0.0031474032115896156	0.7178342124015964
>5k	7.868508028974039E-4	0.9916150762817636
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8740	0.5215288792141503	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7776	0.464005556609752	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2112	0.1260262005606734	No Hit
CTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGCTT	1827	0.10901982406455978	Illumina Single End Adapter 1 (95% over 23bp)
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11480795922288617	0.0
2	0.0	0.0	0.0	0.3025344871413892	0.0
3	0.0	0.0	0.0	0.42396598247328804	0.0
4	0.0	0.0	0.0	0.5754122405334154	0.0
5	0.0	0.0	0.0	0.9777771412818154	0.0
6	0.0	0.0	0.0	1.294573116081349	0.0
7	0.0	0.0	0.0	1.4913100399679684	0.0
8	0.0	0.0	0.0	1.9167678098531962	0.0
9	0.0	0.0	0.0	2.071376657226636	0.0
10	5.967149647759156E-5	0.0	0.0	2.3822054823784105	0.0
11	5.967149647759156E-5	0.0	0.0	2.787673300943645	0.0
12	5.967149647759156E-5	0.0	0.0	3.1306650626968415	0.0
13	5.967149647759156E-5	0.0	0.0	3.2648065867784672	0.0
14	5.967149647759156E-5	0.0	0.0	3.3122454264781527	0.0
15	5.967149647759156E-5	0.0	0.0	3.379554874504876	0.0
16	5.967149647759156E-5	0.0	0.0	3.530285074607272	0.0
17	5.967149647759156E-5	0.0	0.0	3.7067933611879877	0.0
18	5.967149647759156E-5	0.0	0.0	3.9626647380839004	0.0
19	1.1934299295518312E-4	0.0	0.0	4.090839112517767	0.0
20	1.1934299295518312E-4	0.0	0.0	4.233692675085122	0.0
21	1.7901448943277468E-4	0.0	0.0	4.413065193496761	0.0
22	1.7901448943277468E-4	0.0	0.0	4.597629132101952	0.0
23	1.7901448943277468E-4	0.0	0.0	4.818473340565519	0.0
24	4.1770047534314095E-4	0.0	0.0	4.995041298642712	0.0
25	4.1770047534314095E-4	0.0	0.0	5.124946146474429	0.0
26	4.1770047534314095E-4	0.0	0.0	5.255030008795579	0.0
27	4.773719718207325E-4	0.0	0.0	5.376759861609865	0.0
28	4.773719718207325E-4	0.0	0.0	5.50821616835	0.0
29	5.37043468298324E-4	0.0	0.0	5.658051296005232	0.0
30	5.37043468298324E-4	0.0	0.0	5.841242790191438	0.0
31	5.37043468298324E-4	0.0	0.0	6.016318960856692	0.0
32	5.37043468298324E-4	0.0	0.0	6.171464851698429	0.0
33	5.37043468298324E-4	0.0	0.0	6.324044868191631	0.0
34	5.37043468298324E-4	0.0	0.0	6.488618855476829	0.0
35	5.37043468298324E-4	0.0	0.0	6.698483508588518	0.0
36	5.37043468298324E-4	0.0	0.0	6.867473186613058	0.0
37	5.37043468298324E-4	0.0	0.0	7.045353917612759	0.0
38	5.37043468298324E-4	0.0	0.0	7.208316774493061	0.0
39	5.37043468298324E-4	0.0	0.0	7.378440210950674	0.0
40	5.967149647759157E-4	0.0	0.0	7.566763453833953	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	55	1.8189894E-12	46.000004	1
ACGATAT	35	1.01994374E-7	46.000004	1
TACGTCC	70	0.0	46.000004	25
CGGAATA	20	6.3122873E-4	46.0	33
CGTATTC	20	6.3122873E-4	46.0	22
AGTCACG	25	3.4178305E-5	46.0	1
GTCGAAC	25	3.4178305E-5	46.0	12
ACGCATC	20	6.3122873E-4	46.0	39
CTATCGT	30	1.8619176E-6	46.0	35
TTAGCGA	30	1.8619176E-6	46.0	41
GACAATC	20	6.3122873E-4	46.0	21
TAAACGG	20	6.3122873E-4	46.0	2
CAATTCG	30	1.8619176E-6	46.0	1
CGACATA	20	6.3122873E-4	46.0	43
TATGCGC	30	1.8619176E-6	46.0	11
GCGTTGC	20	6.3122873E-4	46.0	29
CGTAGTC	30	1.8619176E-6	46.0	18
GCGATCG	25	3.4178305E-5	46.0	9
TTAACGC	25	3.4178305E-5	46.0	46
ACGCTAA	25	3.4178305E-5	46.0	11
>>END_MODULE