FastQCFastQC Report
Thu 26 May 2016
SRR1544693_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544693_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1429252
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70470.49305510854628853No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52010.3638966396408751No Hit
CTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCTT25310.17708563640281771Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCT17580.1230014021320243No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14580.10201140176819762No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATC351.0197982E-746.00000416
GTGCTAT351.0197982E-746.00000419
TATAGCG551.8189894E-1246.0000041
ACGTATC351.0197982E-746.00000446
TCGCCTA206.311973E-446.016
TAATACG501.6370905E-1146.01
AATCCCG206.311973E-446.038
TCGCAAA253.417577E-546.033
TTGGACG1250.046.01
CCTAGCG301.8617247E-646.01
TTTCGAA301.8617247E-646.016
TAGGTCC301.8617247E-646.017
TAGCGAA253.417577E-546.023
GACCGAC206.311973E-446.010
AAGACTC301.8617247E-646.020
ATAGCCG206.311973E-446.012
TAAACGG900.046.02
CAATTCG206.311973E-446.01
CTAAGCG253.417577E-546.01
CGTTCTA253.417577E-546.041
Produced by FastQC (version 0.11.5)