##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544693_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1429252
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.684148071858566	33.0	31.0	34.0	31.0	34.0
2	33.11110986725924	34.0	33.0	34.0	31.0	34.0
3	33.17541063437378	34.0	33.0	34.0	31.0	34.0
4	36.489763176822564	37.0	37.0	37.0	35.0	37.0
5	36.4844499080638	37.0	37.0	37.0	35.0	37.0
6	36.54352556442111	37.0	37.0	37.0	35.0	37.0
7	36.613194174295366	37.0	37.0	37.0	35.0	37.0
8	36.61417930497911	37.0	37.0	37.0	35.0	37.0
9	38.31953777220532	39.0	39.0	39.0	37.0	39.0
10	38.259389526829416	39.0	39.0	39.0	37.0	39.0
11	38.056423219978	39.0	39.0	39.0	35.0	39.0
12	37.28129119287571	39.0	35.0	39.0	35.0	39.0
13	37.12922003957315	39.0	35.0	39.0	35.0	39.0
14	38.2607363851861	40.0	35.0	41.0	35.0	41.0
15	38.30811011634057	40.0	35.0	41.0	35.0	41.0
16	38.381135027273004	40.0	35.0	41.0	35.0	41.0
17	38.34594878999645	40.0	35.0	41.0	35.0	41.0
18	38.378816331899486	40.0	36.0	41.0	35.0	41.0
19	38.32733975534055	40.0	36.0	41.0	35.0	41.0
20	38.1808806284686	40.0	35.0	41.0	35.0	41.0
21	38.100961202083326	40.0	35.0	41.0	35.0	41.0
22	38.024188876419274	40.0	35.0	41.0	35.0	41.0
23	37.960371578979775	40.0	35.0	41.0	35.0	41.0
24	37.9004171412739	40.0	35.0	41.0	35.0	41.0
25	37.87983924458388	40.0	35.0	41.0	35.0	41.0
26	37.81273631242076	40.0	35.0	41.0	35.0	41.0
27	37.78964171468712	40.0	35.0	41.0	35.0	41.0
28	37.77071992902581	40.0	35.0	41.0	35.0	41.0
29	37.74393738822825	40.0	35.0	41.0	35.0	41.0
30	37.69452552803844	39.0	35.0	41.0	35.0	41.0
31	37.54618499746721	40.0	35.0	41.0	35.0	41.0
32	37.41410751917786	40.0	35.0	41.0	34.0	41.0
33	37.28621404762771	39.0	35.0	41.0	34.0	41.0
34	37.133376759311865	39.0	35.0	41.0	34.0	41.0
35	36.99665279460865	39.0	35.0	41.0	34.0	41.0
36	36.89275649080778	39.0	35.0	41.0	34.0	41.0
37	36.8401597478961	39.0	35.0	41.0	33.0	41.0
38	36.729003702636064	39.0	35.0	41.0	33.0	41.0
39	36.72135984417024	39.0	35.0	41.0	33.0	41.0
40	36.60653054884653	39.0	35.0	41.0	33.0	41.0
41	36.60029372007176	39.0	35.0	41.0	33.0	41.0
42	36.58222412842522	38.0	35.0	41.0	33.0	41.0
43	36.50204932370219	38.0	35.0	41.0	33.0	41.0
44	36.39922700825327	38.0	35.0	41.0	33.0	41.0
45	36.336944079840364	38.0	35.0	41.0	33.0	41.0
46	36.308489335680484	38.0	35.0	41.0	33.0	41.0
47	36.258382706478635	38.0	35.0	41.0	33.0	41.0
48	36.19978492246294	38.0	35.0	41.0	33.0	41.0
49	36.18057347479661	37.0	35.0	41.0	33.0	41.0
50	36.05133034622306	37.0	35.0	40.0	32.0	41.0
51	35.97528497423827	37.0	35.0	40.0	32.0	41.0
52	35.73029388799176	37.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	2.0
13	2.0
14	5.0
15	20.0
16	38.0
17	95.0
18	230.0
19	424.0
20	776.0
21	1281.0
22	2057.0
23	3454.0
24	5885.0
25	9017.0
26	11752.0
27	13132.0
28	12839.0
29	12817.0
30	13502.0
31	15636.0
32	19807.0
33	27361.0
34	76093.0
35	333062.0
36	57020.0
37	67611.0
38	131665.0
39	611209.0
40	2457.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.80695076865381	23.753543811728093	28.689132497278298	14.750372922339796
2	30.91253326915058	25.0431694340816	30.81178126740421	13.23251602936361
3	29.291335607716483	25.54385090942675	31.36969547707472	13.795118005782046
4	25.08074153473285	28.770713632025704	31.57700671400145	14.571538119239994
5	23.157077968056015	33.99820325596885	29.235991973423857	13.608726802551265
6	22.010744081519565	42.2069725982542	24.875109497835233	10.907173822391012
7	87.4134862151671	4.79068771637192	5.93275363616773	1.8630724322932553
8	88.21943226247015	3.8870682007091824	5.960740303319499	1.9327592335011599
9	83.76808288531343	6.076115338652666	7.377845194549317	2.777956581484581
10	49.04705398348227	28.409335792428486	12.487580916451403	10.056029307637841
11	44.45843000394612	21.014418730916592	21.4081211710741	13.119030094063188
12	41.55852152034771	21.497328672620363	23.65817924340844	13.28597056362349
13	20.069029114529837	41.40746348439603	24.123947351481753	14.399560049592374
14	13.781474505545557	44.46731577076681	26.488680792470465	15.262528931217167
15	13.707029970921852	24.404793556349755	48.2717533367104	13.616423136018001
16	15.996059477265032	20.17097054963016	46.657482375396356	17.175487597708454
17	15.274213364753031	19.84954367739209	29.26027040717802	35.61597255067686
18	18.53438022126259	24.347840688695904	37.542994517411906	19.5747845726296
19	27.299874339864488	25.248801470979227	27.42679387539776	20.024530313758525
20	31.075275738638116	23.4281288394209	27.485076109741318	18.011519312199667
21	20.782619160232066	29.214722106388518	29.522645411725858	20.480013321653566
22	20.62113609076636	24.462655990686034	26.429209124772957	28.48699879377465
23	16.46000845197348	31.28671500896973	26.22910445463781	26.024172084418986
24	17.042201095398152	23.57456907529253	42.2763095661227	17.106920263186616
25	15.495867768595042	26.953959133868626	37.8119463887404	19.73822670879593
26	15.105593695163625	36.12092199276265	28.237987422791782	20.535496889281944
27	15.220758830493153	38.47110236683244	28.45761279326529	17.85052600940912
28	13.239022929476397	32.145765757193274	37.71413298704498	16.901078326285358
29	14.819499990204665	25.21647687041893	37.00145250802518	22.962570631351223
30	16.732808490035346	34.82716833700425	30.411082160458758	18.028941012501644
31	27.43050210879537	30.274297324754485	26.060904585055678	16.234295981394464
32	28.624763162829225	26.59614959433326	28.240296322831803	16.53879092000571
33	28.198106422100512	30.121700022109465	23.925521881375715	17.75467167441431
34	19.08047006406148	28.55773509500074	27.460517809315643	24.901277031622136
35	18.082115680090006	27.663351179498086	32.226857125265525	22.027676015146383
36	30.310400125380273	26.470279558818177	26.394365724169006	16.824954591632547
37	18.507163187457497	36.259176128492385	30.295217358450433	14.938443325599685
38	19.50068987134529	33.97028655548497	25.354661039480792	21.17436253368895
39	19.8267345436634	34.57920646603958	27.50935454349548	18.084704446801545
40	24.718034328445928	26.731395163344185	26.713903496374325	21.836667011835562
41	16.63310598830717	25.59359721028902	29.688746281271598	28.084550520132208
42	20.20658358358078	24.975371732906442	26.79786349782963	28.02018118568314
43	19.567368105834383	24.88161639794802	29.355634975497676	26.19538052071993
44	16.872461959122674	29.245647373591222	31.762068550542523	22.119822116743585
45	15.574860136630909	40.57639940332425	25.043659200756757	18.805081259288077
46	21.098448699039775	34.085311757478735	27.437778642254827	17.378460901226656
47	18.90506362768777	29.648655380576695	27.9064153837112	23.53986560802434
48	20.68774435858757	25.82294794759776	34.1293207915749	19.359986902239772
49	21.29295603574457	25.15343690265957	34.60159579976099	18.952011261834862
50	19.56268033908646	34.41716366323084	28.591808862258024	17.428347135424684
51	17.59668693834257	34.52729119847305	26.046771318144035	21.829250545040342
52	19.01085322952146	27.44246640900275	35.10332677512433	18.443353586351463
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6087.0
1	5739.0
2	5391.0
3	9086.0
4	12781.0
5	9030.5
6	5280.0
7	5481.0
8	5682.0
9	5792.5
10	5903.0
11	5991.0
12	6079.0
13	5929.5
14	5768.5
15	5757.0
16	5025.5
17	4294.0
18	4699.5
19	5105.0
20	5506.0
21	5907.0
22	5802.0
23	5697.0
24	7642.5
25	9588.0
26	11118.5
27	12649.0
28	17151.0
29	21653.0
30	21634.5
31	21616.0
32	28113.0
33	34610.0
34	40006.0
35	45402.0
36	49466.5
37	53531.0
38	58628.0
39	83259.5
40	102794.0
41	123476.0
42	144158.0
43	155244.5
44	166331.0
45	161449.5
46	156568.0
47	136592.5
48	116617.0
49	105314.5
50	94012.0
51	83524.5
52	73037.0
53	64628.5
54	56220.0
55	49167.0
56	42114.0
57	36765.0
58	31416.0
59	29954.0
60	28492.0
61	26534.5
62	24577.0
63	19810.5
64	12879.5
65	10715.0
66	9016.5
67	7318.0
68	6538.5
69	5759.0
70	4990.0
71	4221.0
72	3654.0
73	3087.0
74	2438.5
75	1790.0
76	1400.0
77	1010.0
78	900.5
79	791.0
80	599.0
81	407.0
82	304.0
83	201.0
84	112.5
85	24.0
86	23.5
87	23.0
88	12.0
89	3.5
90	6.0
91	4.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1429252.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.729513598260425
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.49493070119819	12.032280526624858
2	9.584222498090798	3.015103162249454
3	3.284541923062936	1.549927405286247
4	1.5556092941387034	0.9787591018294015
5	0.9491510929496665	0.7464842511677762
6	0.6317029977710233	0.5961828536100712
7	0.46814419291948434	0.5154576313931576
8	0.3567110239227057	0.448871272115328
9	0.28454073309835143	0.4028118597476559
>10	3.7159989363022548	14.438442826862449
>50	1.081045117810889	11.943948213998299
>100	1.5741769477654228	50.402319402133735
>500	0.016542046880806772	1.5758877987858733
>1k	0.001788329392519651	0.49219682733287196
>5k	8.941646962598255E-4	0.8613268668628398
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7047	0.49305510854628853	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5201	0.3638966396408751	No Hit
CTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCTT	2531	0.17708563640281771	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCT	1758	0.1230014021320243	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1458	0.10201140176819762	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.18597140322350433	0.0
2	0.0	0.0	0.0	0.43736164091426843	0.0
3	0.0	0.0	0.0	0.5901688435629266	0.0
4	0.0	0.0	0.0	0.7398975128248902	0.0
5	0.0	0.0	0.0	1.1370982863763703	0.0
6	0.0	0.0	0.0	1.4395641916191126	0.0
7	0.0	0.0	0.0	1.6372200283784806	0.0
8	0.0	0.0	0.0	2.0443560687688387	0.0
9	0.0	0.0	0.0	2.2012213381545034	0.0
10	0.0	0.0	0.0	2.5162812436155417	0.0
11	0.0	0.0	0.0	2.972883718196651	0.0
12	0.0	0.0	0.0	3.364417191649898	0.0
13	0.0	0.0	0.0	3.528628961162902	0.0
14	0.0	0.0	0.0	3.5888702622070845	0.0
15	0.0	0.0	0.0	3.681855963818837	0.0
16	0.0	0.0	0.0	3.891965867460742	0.0
17	0.0	0.0	0.0	4.12355553814163	0.0
18	0.0	0.0	0.0	4.464013344042898	0.0
19	0.0	0.0	0.0	4.629554480245611	0.0
20	1.399333357588445E-4	0.0	0.0	4.792296949733148	0.0
21	1.399333357588445E-4	0.0	0.0	4.995200286583471	0.0
22	0.0014693000254678672	0.0	0.0	5.206219756907809	0.0
23	0.0014693000254678672	0.0	0.0	5.446695194409384	0.0
24	0.0018191333648649784	0.0	0.0	5.634205864326235	0.0
25	0.0018891000327444006	0.0	0.0	5.783374800245163	0.0
26	0.0018891000327444006	0.0	0.0	5.932193902824694	0.0
27	0.0018891000327444006	0.0	0.0	6.073736471944765	0.0
28	0.0019590667006238226	0.0	0.0	6.230461807994671	0.0
29	0.0019590667006238226	0.0	0.0	6.39656267754042	0.0
30	0.0019590667006238226	0.0	0.0	6.6045036144780624	0.0
31	0.0019590667006238226	0.0	0.0	6.798801051179218	0.0
32	0.0019590667006238226	0.0	0.0	6.972458320855944	0.0
33	0.0019590667006238226	0.0	0.0	7.1533921239921305	0.0
34	0.0019590667006238226	0.0	0.0	7.337754293854408	0.0
35	0.0019590667006238226	0.0	0.0	7.57962906471357	0.0
36	0.0019590667006238226	0.0	0.0	7.779034068169924	0.0
37	0.0019590667006238226	0.0	0.0	7.976759871597171	0.0
38	0.0019590667006238226	0.0	0.0	8.166579441554044	0.0
39	0.0019590667006238226	0.0	0.0	8.366963978360708	0.0
40	0.0019590667006238226	0.0	0.0	8.590087682228187	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCATC	35	1.0197982E-7	46.000004	16
GTGCTAT	35	1.0197982E-7	46.000004	19
TATAGCG	55	1.8189894E-12	46.000004	1
ACGTATC	35	1.0197982E-7	46.000004	46
TCGCCTA	20	6.311973E-4	46.0	16
TAATACG	50	1.6370905E-11	46.0	1
AATCCCG	20	6.311973E-4	46.0	38
TCGCAAA	25	3.417577E-5	46.0	33
TTGGACG	125	0.0	46.0	1
CCTAGCG	30	1.8617247E-6	46.0	1
TTTCGAA	30	1.8617247E-6	46.0	16
TAGGTCC	30	1.8617247E-6	46.0	17
TAGCGAA	25	3.417577E-5	46.0	23
GACCGAC	20	6.311973E-4	46.0	10
AAGACTC	30	1.8617247E-6	46.0	20
ATAGCCG	20	6.311973E-4	46.0	12
TAAACGG	90	0.0	46.0	2
CAATTCG	20	6.311973E-4	46.0	1
CTAAGCG	25	3.417577E-5	46.0	1
CGTTCTA	25	3.417577E-5	46.0	41
>>END_MODULE