FastQCFastQC Report
Fri 17 Jun 2016
SRR1528191_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528191_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1283184
Sequences flagged as poor quality0
Sequence length52
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGGATGGGATTCGCCAGCACCGCCACCACGATGATGACGCCGCGTACC35630.2776686741729947No Hit
TAGTTGGATGGGGGGTGAAACCCCGTCTCTACTAAAAATACAAAACATTAGC27110.2112713375478497No Hit
GGGATGGATGGGGACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGAT25400.19794511153505656No Hit
CGATTGGATGGGGGCAGCCTGATACTGCCACATGCTGCACAGTGCCCACCCA25310.19724373121859373No Hit
TTTTTGGATGGGGCAGGCAGGAGGATCTCTTAGTAGCTTAGTAGAGGTCAGC23750.18508647239990522No Hit
ACTGTGGATGGGGGTAGCAAGCAGGGAGGGATCCGCGGCCATCATCAGCGAC22940.17877404955174003No Hit
GACGTGGATGGGGCCTAGGACTAGCCAGCTAGCCTGGCATCACTCATTCCAA22390.17448783650668961No Hit
AGGCTGGATGGGGTACTACTTCATTGCAACTTTCATCGCTGACCACATCAGA21410.16685058417187246No Hit
GGGATGGATGGGGAATCCCAGCCCTTTGGGAGTCCAAGGCAGGTGGATAGCC20970.1634216137358321No Hit
GGTCTGGATGGGGGCGCCCCAGTTCCCCTGACCATCAACCAGCGGATAACGG19390.15110849262459633No Hit
GTACTGGATGGGGGTACCAACTCCACAGCCCACACCTTCTAATAGTGCCACT19310.15048504345440716No Hit
CTTTTGGATGGGGTTCCCAGTCTCTGTTTCTTAGCCCAGGAAAAGAATTCCT18230.14206847965685357No Hit
TGATTGGATGGGGGAGGCCAAGATGTTCAGATCGCTTGAGCTCAGGAGTTTG17770.13848364692826592No Hit
CGTTTGGATGGGGAGTTCCTAAGGGCGCATGGTGGATGCCTTGGCAACAGGA17590.13708088629534035No Hit
GTGGTGGATGGGGAACTGCCAACGTCACTGAGGGCCCTGCTCTGAAGCCCTG17420.13575605680868838No Hit
AGTGTGGATGGGGTCAAACTCTCAATTAACCATGGAAGACATGCACTGCATT17390.13552226336986745No Hit
TAACTGGATGGGGGCTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCCT17350.13521053878477288No Hit
TAAGTGGATGGGGTTACCCTACTCCACTATGCCCTCATCTTAACTAATTATA17120.13341812242047907No Hit
GTCTTGGATGGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACT17040.1327946732502899No Hit
TGGGTGGATGGGAGTTTCTGATATTCCCTGACACAATTAAGGAATGCCTTTA16860.13139191261736433No Hit
TTTGTGGATGGGAGCACACAAGTGTTCATAGTAGTTTCACTTGTAGTAGCCA16780.13076846344717516No Hit
TCCATGGATGGGGGTTCAAGAGATTCTCGTGCCTCAGCCTCCCAAGTAGCTG16520.12874225364406042No Hit
ATGCTGGATGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTG16500.12858639135151312No Hit
TCTGTGGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16150.12585880123193555No Hit
ATGCTGGATGGGGGCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGT16110.12554707664684098No Hit
ACTCTGGATGGGGTCACAGGGGCATGTGTTCCACTATGTTCATAATGGCCTT15780.12297534881981073No Hit
TTTCTGGATGGGGTTGATGAACTTGGAGTTCCCGTGTTCCTTTAATTAGCCT15530.12102707016296962No Hit
GCTATGGATGGGGCTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTA15290.11915672265240215No Hit
TAGATGGATGTGGGGCCAATATGGTGAAAACCAGACTCTACAAAAAGCACAA15270.11900086035985485No Hit
TTCGTGGATGGGGTGACCAATGGAAAGAACTTAGTGCTGGTGTGTACCACTG15200.11845534233593934No Hit
TATTTGGATGGGGGGAGTCACCCCTCAAGGATGGCCTCTGGGGTTTCTGCTC15130.11790982431202383No Hit
CTGTTGGATGGGGAGGCTGGCCTTGAACTCAGAAATCCACCTGCCTCTGCTT14420.1123767129265951No Hit
CCTGTGGATGGGGGAGTGCTGCCTTTGCTCCGCACCATGAACCACACTTCTC14030.10933739822192297No Hit
CAAGTGGATGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAACACCGACTG13970.1088698113442811No Hit
TAATTGGATGGGGGAGCCTCAGGGCTCCAGATCGGTTAGCTGCATCCCACCT13870.10809049988154465No Hit
CTTCTGGATGGGGGTCAAGAAAGTCAATGAGGAAATTAAATGTAAATTCTGA13710.10684360154116636No Hit
TATCTGGATGGGGACAGCAAGTTGTTGTTCTAGAACTTTGAACACTAAATGG13480.10505118517687252No Hit
ATGGTGGATGGGGCAGATGCCCGCCACGCAGCGCGTTCTGTTTCTCTGGCGC13380.1042718737141361No Hit
TGTCTGGATGGGCTCTTCGGTTCTCATCGCTGTGCGGACGCCAGAGCCGAGC13220.10302497537375778No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCAG351.0196891E-746.00000439
AGGTCAA351.0196891E-746.00000433
AAACTTA700.046.00000446
TGTCAGG700.046.00000420
CATGGAC1400.046.00000435
CGTGGCC351.0196891E-746.00000423
GACAGGG351.0196891E-746.00000415
TAATAAG351.0196891E-746.00000422
TCTTGTT351.0196891E-746.00000433
CCTTAGG1400.046.00000425
GATACGT351.0196891E-746.00000413
TCTGACT351.0196891E-746.00000444
CTGTCCA700.046.00000438
TCCATCA351.0196891E-746.00000437
CGAACCT351.0196891E-746.00000422
ACGCCTG351.0196891E-746.00000437
CCCTCAA1450.046.00000422
CTACTAA2900.046.00000429
TTGTGCA351.0196891E-746.00000426
TTTACGA700.046.00000426
Produced by FastQC (version 0.11.5)