FastQCFastQC Report
Fri 17 Jun 2016
SRR1528190_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528190_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1279552
Sequences flagged as poor quality0
Sequence length52
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGGATGGGATTCGCCAGCACCGCCACCACGATGATGACGCCGCGTACC35010.2736113889861452No Hit
TAGTTGGATGGGGGGTGAAACCCCGTCTCTACTAAAAATACAAAACATTAGC26920.21038613514730153No Hit
GGGATGGATGGGGACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGAT25000.19538088330915818No Hit
CGATTGGATGGGGGCAGCCTGATACTGCCACATGCTGCACAGTGCCCACCCA24300.18991021857650175No Hit
TTTTTGGATGGGGCAGGCAGGAGGATCTCTTAGTAGCTTAGTAGAGGTCAGC23310.18217313559745912No Hit
ACTGTGGATGGGGGTAGCAAGCAGGGAGGGATCCGCGGCCATCATCAGCGAC22800.17818736557795228No Hit
AGGCTGGATGGGGTACTACTTCATTGCAACTTTCATCGCTGACCACATCAGA22150.17310746261191418No Hit
GACGTGGATGGGGCCTAGGACTAGCCAGCTAGCCTGGCATCACTCATTCCAA21760.1700595208322913No Hit
GGGATGGATGGGGAATCCCAGCCCTTTGGGAGTCCAAGGCAGGTGGATAGCC20650.16138460961336468No Hit
GGTCTGGATGGGGGCGCCCCAGTTCCCCTGACCATCAACCAGCGGATAACGG19320.15099034662131747No Hit
GTACTGGATGGGGGTACCAACTCCACAGCCCACACCTTCTAATAGTGCCACT18980.14833316660831292No Hit
CTTTTGGATGGGGTTCCCAGTCTCTGTTTCTTAGCCCAGGAAAAGAATTCCT18430.14403478717551144No Hit
TAAGTGGATGGGGTTACCCTACTCCACTATGCCCTCATCTTAACTAATTATA17410.13606324713649778No Hit
TTTGTGGATGGGAGCACACAAGTGTTCATAGTAGTTTCACTTGTAGTAGCCA17410.13606324713649778No Hit
GTGGTGGATGGGGAACTGCCAACGTCACTGAGGGCCCTGCTCTGAAGCCCTG17220.13457835242334817No Hit
TGATTGGATGGGGGAGGCCAAGATGTTCAGATCGCTTGAGCTCAGGAGTTTG17080.13348421947681688No Hit
GTCTTGGATGGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACT16950.13246823888360926No Hit
CGTTTGGATGGGGAGTTCCTAAGGGCGCATGGTGGATGCCTTGGCAACAGGA16900.13207747711699094No Hit
ATGCTGGATGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTG16450.1285606212174261No Hit
AGTGTGGATGGGGTCAAACTCTCAATTAACCATGGAAGACATGCACTGCATT16080.12566898414445055No Hit
TTTCTGGATGGGGTTGATGAACTTGGAGTTCCCGTGTTCCTTTAATTAGCCT16010.1251219176711849No Hit
TTCGTGGATGGGGTGACCAATGGAAAGAACTTAGTGCTGGTGTGTACCACTG15960.1247311559045666No Hit
TGGGTGGATGGGAGTTTCTGATATTCCCTGACACAATTAAGGAATGCCTTTA15860.12394963237132997No Hit
TAACTGGATGGGGGCTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCCT15820.1236370229580353No Hit
ATGCTGGATGGGGGCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGT15570.12168321412494372No Hit
TCCATGGATGGGGGTTCAAGAGATTCTCGTGCCTCAGCCTCCCAAGTAGCTG15550.12152690941829641No Hit
TATTTGGATGGGGGGAGTCACCCCTCAAGGATGGCCTCTGGGGTTTCTGCTC15540.12144875706497274No Hit
TCTGTGGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15470.1209016905917071No Hit
ACTCTGGATGGGGTCACAGGGGCATGTGTTCCACTATGTTCATAATGGCCTT15180.11863527234532087No Hit
TAGATGGATGTGGGGCCAATATGGTGAAAACCAGACTCTACAAAAAGCACAA14710.11496211173910868No Hit
GCTATGGATGGGGCTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTA14450.11293015055269344No Hit
CAAGTGGATGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAACACCGACTG13910.10870992347321562No Hit
CTTCTGGATGGGGGTCAAGAAAGTCAATGAGGAAATTAAATGTAAATTCTGA13880.10847546641324464No Hit
TATCTGGATGGGGACAGCAAGTTGTTGTTCTAGAACTTTGAACACTAAATGG13830.10808470464662631No Hit
TGTCTGGATGGGCTCTTCGGTTCTCATCGCTGTGCGGACGCCAGAGCCGAGC13710.10714687640674236No Hit
ATGGTGGATGGGGCAGATGCCCGCCACGCAGCGCGTTCTGTTTCTCTGGCGC13560.10597459110688742No Hit
TAATTGGATGGGGGAGCCTCAGGGCTCCAGATCGGTTAGCTGCATCCCACCT13520.10566198169359275No Hit
CTGTTGGATGGGGAGGCTGGCCTTGAACTCAGAAATCCACCTGCCTCTGCTT13150.10277034462061721No Hit
GTAATGGATGGGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCAT13070.10214512579402792No Hit
GATATGGATGGGAGGAGGGGGAGGAGAAGCTTAGATACTATGGTTTCTAAAT12990.10151990696743861No Hit
CCTGTGGATGGGGGAGTGCTGCCTTTGCTCCGCACCATGAACCACACTTCTC12960.10128544990746763No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGAT1150.046.00000442
AGATACT1150.046.00000433
TTAGCAA1150.046.00000430
CGGCATT1450.046.00000444
TTAGAGA1150.046.00000441
ATACTAT1150.046.00000435
TATTGGT1150.046.00000423
GCATTTA1450.046.00000446
CTAACAC1150.046.00000443
CCGTACA1150.046.00000427
CGCTCCG1150.046.00000423
CACTACA1450.046.00000435
TCTAACA1150.046.00000442
ACTACAC1450.046.00000436
TTGGTCA1150.046.00000425
ACTGTCG1150.046.00000427
TCCGTAC1150.046.00000426
CGTACAG1150.046.00000428
ATTGGTC1150.046.00000424
GCGGCAT1450.046.00000443
Produced by FastQC (version 0.11.5)