FastQCFastQC Report
Fri 17 Jun 2016
SRR1528189_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528189_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1149368
Sequences flagged as poor quality0
Sequence length59
%GC40

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTGGATGGGATTCGCCAGCACCGCCACCACGATGATGACGCCGCGTACCACCTGCT29230.25431367499356167No Hit
TAGTTGGATGGGGGGTGAAACCCCGTCTCTACTAAAAATACAAAACATTAGCCAGGTGT22180.19297561790479636No Hit
GGGATGGATGGGGACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCC20440.17783686338927132No Hit
GACGTGGATGGGGCCTAGGACTAGCCAGCTAGCCTGGCATCACTCATTCCAACAGGGCC20040.17435668993742648No Hit
ACTGTGGATGGGGGTAGCAAGCAGGGAGGGATCCGCGGCCATCATCAGCGACACCATCA19790.17218158153002344No Hit
CGATTGGATGGGGGCAGCCTGATACTGCCACATGCTGCACAGTGCCCACCCATCAGAAG18690.16261110453745015No Hit
GGGATGGATGGGGAATCCCAGCCCTTTGGGAGTCCAAGGCAGGTGGATAGCCTGAGGTC17910.1558247663063527No Hit
AGGCTGGATGGGGTACTACTTCATTGCAACTTTCATCGCTGACCACATCAGACACCATG16770.14590627196859493No Hit
TTTTTGGATGGGGCAGGCAGGAGGATCTCTTAGTAGCTTAGTAGAGGTCAGCCTGGTCT16620.1446012069241531No Hit
CGTTTGGATGGGGAGTTCCTAAGGGCGCATGGTGGATGCCTTGGCAACAGGAGCCGATG16590.14434019391526473No Hit
GGTCTGGATGGGGGCGCCCCAGTTCCCCTGACCATCAACCAGCGGATAACGGTAAGAGA16420.14286112019823066No Hit
TAAGTGGATGGGGTTACCCTACTCCACTATGCCCTCATCTTAACTAATTATATCTGCAA15810.1375538556841673No Hit
GTGGTGGATGGGGAACTGCCAACGTCACTGAGGGCCCTGCTCTGAAGCCCTGGGCCCTG15420.1341606865686186No Hit
TAGATGGATGTGGGGCCAATATGGTGAAAACCAGACTCTACAAAAAGCACAAAAATTCG15200.13224659117010393No Hit
CTTTTGGATGGGGTTCCCAGTCTCTGTTTCTTAGCCCAGGAAAAGAATTCCTAGGCTCT14990.1304195001078854No Hit
TTTGTGGATGGGAGCACACAAGTGTTCATAGTAGTTTCACTTGTAGTAGCCAAAAACAG14480.1259822789567832No Hit
ATGCTGGATGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCT14330.12467721391234139No Hit
AGTGTGGATGGGGTCAAACTCTCAATTAACCATGGAAGACATGCACTGCATTATATACT14160.12319814019530734No Hit
ACTCTGGATGGGGTCACAGGGGCATGTGTTCCACTATGTTCATAATGGCCTTATTTGTG13850.1205010057701276No Hit
TGGGTGGATGGGAGTTTCTGATATTCCCTGACACAATTAAGGAATGCCTTTAGGCCCTA13670.11893492771679741No Hit
GTACTGGATGGGGGTACCAACTCCACAGCCCACACCTTCTAATAGTGCCACTTCCTGAG13630.11858691037161292No Hit
TGATTGGATGGGGGAGGCCAAGATGTTCAGATCGCTTGAGCTCAGGAGTTTGAGACCAG13400.11658581063680215No Hit
TAACTGGATGGGGGCTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCCTCTCGCAA13340.11606378461902542No Hit
ATGCTGGATGGGGGCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCC13070.11371466753903016No Hit
GTCTTGGATGGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC13050.1135406588664379No Hit
GCTATGGATGGGGCTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAGT12800.11136555045903487No Hit
TATTTGGATGGGGGGAGTCACCCCTCAAGGATGGCCTCTGGGGTTTCTGCTCATGCCCC12610.10971246806940858No Hit
TCCATGGATGGGGGTTCAAGAGATTCTCGTGCCTCAGCCTCCCAAGTAGCTGGGACTAC12240.10649330762645211No Hit
TTCGTGGATGGGGTGACCAATGGAAAGAACTTAGTGCTGGTGTGTACCACTGATATACT12050.10484022523682579No Hit
TTTCTGGATGGGGTTGATGAACTTGGAGTTCCCGTGTTCCTTTAATTAGCCTATGATTT11890.10344815585608787No Hit
CTTCTGGATGGGGGTCAAGAAAGTCAATGAGGAAATTAAATGTAAATTCTGAGCAGCCA11880.10336115151979175No Hit
CAAGTGGATGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAACACCGACTGCTCTTCC11850.10310013851090338No Hit
TCTGTGGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11720.1019690821390538No Hit
TATCTGGATGGGGACAGCAAGTTGTTGTTCTAGAACTTTGAACACTAAATGGAAGAAAA11570.100664017094612No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACG750.053.00000449
TTGGCTT750.053.00000439
TCTGATA1750.053.00000417
GTTTCAC1500.053.00000434
TCCTCAG750.053.00000452
CGACACG750.053.00000448
TAACGAA750.053.00000451
GCGCACT1550.053.00000432
CATTAGC3100.053.00000446
CACTACA1550.053.00000435
TCTAAAT1350.053.00000446
ACTACAC1550.053.00000436
TTAACGA750.053.00000450
ACGTCAC1500.053.00000422
CGCACTA1550.053.00000433
TTTGAGG750.053.00000428
CGCAATT1550.053.00000420
GCTAACT1500.053.00000438
TATACAA750.053.00000441
ATTTGAC750.053.00000415