##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528189_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1149368
Sequences flagged as poor quality	0
Sequence length	59
%GC	40
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.345685628971747	31.0	30.0	31.0	27.0	33.0
2	31.152934482254597	31.0	31.0	34.0	30.0	34.0
3	31.481138329934364	31.0	31.0	34.0	30.0	34.0
4	35.34543244635313	35.0	35.0	37.0	33.0	37.0
5	34.829428868734816	35.0	35.0	37.0	32.0	37.0
6	35.00903453028099	35.0	35.0	37.0	32.0	37.0
7	34.84029484029484	37.0	35.0	37.0	32.0	37.0
8	33.58611950219599	35.0	33.0	36.0	28.0	37.0
9	36.43079849099679	37.0	35.0	39.0	32.0	39.0
10	36.54315415080288	38.0	35.0	39.0	32.0	39.0
11	37.12696281782684	39.0	37.0	39.0	34.0	39.0
12	37.25777035727461	39.0	37.0	39.0	35.0	39.0
13	36.8768862540109	39.0	37.0	39.0	33.0	39.0
14	35.97092315080984	38.0	35.0	40.0	27.0	40.0
15	34.99229663606434	37.0	33.0	40.0	27.0	40.0
16	35.27866705876621	38.0	34.0	40.0	27.0	40.0
17	35.209420307508125	38.0	33.0	40.0	26.0	40.0
18	35.74566631400909	38.0	34.0	40.0	29.0	40.0
19	36.08816236401222	38.0	34.0	40.0	30.0	40.0
20	36.145244168969384	38.0	35.0	40.0	30.0	40.0
21	36.15825653750583	38.0	35.0	40.0	30.0	40.0
22	36.074833299691655	38.0	35.0	40.0	30.0	40.0
23	35.956191576588175	38.0	34.0	40.0	30.0	40.0
24	35.94655323621329	38.0	34.0	40.0	29.0	40.0
25	35.87872378559347	38.0	34.0	40.0	29.0	40.0
26	35.441593989044414	38.0	34.0	40.0	27.0	40.0
27	34.877213390315376	37.0	33.0	40.0	25.0	40.0
28	34.617787340521055	37.0	33.0	39.0	25.0	40.0
29	34.384091083099584	36.0	33.0	40.0	26.0	40.0
30	34.15002331716213	35.0	33.0	40.0	25.0	40.0
31	34.022089530942225	35.0	33.0	40.0	25.0	40.0
32	33.803860034384115	35.0	33.0	39.0	24.0	40.0
33	33.35705187546547	35.0	32.0	39.0	21.0	40.0
34	33.169777651718164	35.0	32.0	39.0	21.0	40.0
35	33.0648608626654	35.0	32.0	39.0	21.0	40.0
36	32.64720872688295	35.0	31.0	39.0	18.0	40.0
37	32.491747638702314	35.0	31.0	39.0	18.0	40.0
38	32.15478506448761	35.0	31.0	39.0	16.0	40.0
39	31.56872820541376	35.0	30.0	39.0	15.0	40.0
40	31.054756179047963	35.0	29.0	38.0	11.0	40.0
41	30.384753186098795	35.0	26.0	38.0	10.0	40.0
42	29.798325688552318	34.0	24.0	38.0	9.0	40.0
43	29.235063965588044	34.0	23.0	37.0	8.0	40.0
44	28.731421094027326	34.0	22.0	37.0	8.0	40.0
45	28.515922663585552	33.0	21.0	37.0	8.0	40.0
46	28.149525652358513	33.0	20.0	37.0	8.0	40.0
47	27.67772462779545	33.0	18.0	36.0	8.0	40.0
48	27.297117198321164	33.0	18.0	35.0	8.0	40.0
49	26.9252006319995	33.0	15.0	35.0	8.0	39.0
50	26.409987053754758	33.0	13.0	35.0	8.0	39.0
51	26.080841819156266	32.0	12.0	35.0	8.0	39.0
52	25.48155421066186	31.0	10.0	35.0	8.0	39.0
53	25.25522635048131	31.0	10.0	35.0	8.0	38.0
54	24.98893478850986	31.0	10.0	35.0	8.0	38.0
55	24.694234570651002	30.0	10.0	35.0	8.0	38.0
56	24.200854730599772	30.0	10.0	35.0	8.0	38.0
57	23.660529090769884	28.0	9.0	35.0	8.0	37.0
58	23.2303317997369	27.0	9.0	35.0	8.0	37.0
59	22.420318818689925	26.0	9.0	34.0	8.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	1.0
13	4.0
14	27.0
15	96.0
16	321.0
17	1036.0
18	2562.0
19	5112.0
20	8896.0
21	13915.0
22	20213.0
23	28065.0
24	36244.0
25	45819.0
26	55327.0
27	62723.0
28	59805.0
29	52653.0
30	53593.0
31	61241.0
32	70118.0
33	81909.0
34	95974.0
35	99603.0
36	113053.0
37	97034.0
38	75697.0
39	8325.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.80304306366629	22.820193358436985	37.52740636593328	7.849357211963444
2	20.700245700245702	22.264322653841067	36.05973021695401	20.97570142895922
3	19.78469906940162	24.47492883045291	37.02486931948688	18.71550278065859
4	19.478443805639273	21.706276840837745	36.81623292104879	21.999046432474195
5	0.0	0.0	100.0	0.0
6	100.0	0.0	0.0	0.0
7	100.0	0.0	0.0	0.0
8	0.0	100.0	0.0	0.0
9	0.0	0.0	100.0	0.0
10	100.0	0.0	0.0	0.0
11	98.85458791266156	0.6954256600148951	0.35201954465410557	0.09796688266943225
12	98.00838373784549	1.3176806732047526	0.3080823548245645	0.3658532341251888
13	88.61791871706886	7.218749782489159	2.361558700085612	1.8017728003563698
14	33.26436789609594	42.98597142081561	12.604666216564233	11.144994466524212
15	21.707668910218487	50.572401528492186	16.636969186544256	11.082960374745078
16	28.894401096950673	41.05621524176765	17.912713769654278	12.136669891627399
17	17.01152285429906	43.81921194952356	18.8677603691768	20.301504827000578
18	16.83812321206089	45.314555477445	21.125000870043362	16.72232044045075
19	20.14742014742015	43.79250161828065	23.11470303679935	12.945375197499843
20	17.585751473853456	44.41353857076237	23.283491449213827	14.717218506170349
21	24.60230317879043	42.93881507054312	14.890966165753703	17.567915584912754
22	23.593574903773202	42.84250127026331	18.886292292807873	14.677631533155614
23	21.199215568903952	44.26641423808563	20.567303074385226	13.967067118625193
24	22.03384816699264	46.20391380306395	17.75184275184275	14.01039527810066
25	23.74861663105289	43.818254901824304	18.217228946690703	14.2158995204321
26	22.240831483041116	45.01064933076265	17.402085319932343	15.346433866263895
27	19.176538758691734	49.650503581098484	17.030663808284206	14.14229385192558
28	20.770197186627783	45.82413987513137	17.067640651210056	16.338022287030785
29	23.683711396175987	46.7299420203103	14.078606677756818	15.507739905756903
30	25.2646671910128	47.30939090004246	14.298814652922301	13.127127256022439
31	19.52560015591177	51.28940426390851	15.082636718614056	14.102358861565662
32	20.19379345866598	53.87743525137293	14.823624809460503	11.105146480500588
33	19.875270583485882	54.12600664017094	13.583726012904481	12.41499676343869
34	19.332450529334384	56.18339818056533	12.32346820165517	12.160683088445127
35	19.31339657968553	54.90939368418122	14.130461262189309	11.64674847394394
36	18.27700092572614	54.45557906606066	16.6830814847812	10.584338523432008
37	18.592913670817353	54.21205392876781	16.651324902033117	10.54370749838172
38	17.16351942980838	54.41077183286816	17.59514794217344	10.830560795150031
39	17.39294986462125	54.711110801762366	16.053866124687655	11.842073208928733
40	20.108442204759484	53.009131975137635	14.404698930194682	12.477726889908194
41	21.370179089725834	51.00516109722909	15.363051694496455	12.261608118548628
42	23.222066387788768	51.17908276548503	13.52639015528534	12.072460691440861
43	23.333866959929285	49.65285269817848	14.401305761079133	12.611974580813106
44	20.7474020505182	50.06264312213321	15.16824898552944	14.021705841819157
45	20.930546178421533	47.844554572599904	16.873707985606	14.351191263372568
46	23.705723493258905	48.31672710567895	13.701007858231653	14.276541542830495
47	23.425569530385395	47.04124353557781	15.18860800022273	14.344578933814061
48	23.740612232113648	47.418929359439275	14.551040223844755	14.28941818460232
49	21.76839793695318	48.68510346555672	13.928872214991195	15.617626382498903
50	19.737977740810603	49.423248254693014	14.691204209617808	16.147569794878578
51	19.929648293670958	48.46254637331124	16.86274543923269	14.745059893785106
52	21.044434854633153	47.07839438717626	16.412062977218785	15.465107780971804
53	18.739167960131134	47.96670866075966	16.214911151171773	17.07921222793744
54	18.03312777108811	47.71143793806683	17.105313528826276	17.150120762018776
55	18.87315463802716	45.782029776364055	16.428593801115046	18.91622178449374
56	21.024510861621344	43.32859449714974	18.544017233818934	17.102877407409984
57	19.44599118820082	41.93722115110217	19.018886901323164	19.59790075937385
58	21.08349980163011	40.79476721119781	18.102470227116118	20.019262760055963
59	21.678087435877806	38.20360406762673	20.641343764573225	19.476964731922237
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	1.0
6	17.0
7	33.0
8	537.5
9	3555.0
10	6068.0
11	10590.5
12	15113.0
13	19208.5
14	18024.0
15	12744.0
16	10393.5
17	8043.0
18	7442.5
19	7187.5
20	7533.0
21	9505.5
22	11478.0
23	14504.0
24	17530.0
25	23246.5
26	33308.0
27	37653.0
28	42449.0
29	47245.0
30	51680.0
31	59844.0
32	63573.0
33	64021.5
34	64470.0
35	59925.5
36	56239.5
37	57098.0
38	51081.0
39	45064.0
40	49369.5
41	53675.0
42	48750.0
43	44406.5
44	44988.0
45	47477.0
46	49966.0
47	47693.0
48	45476.5
49	45533.0
50	45944.5
51	46356.0
52	45889.0
53	44252.5
54	43083.0
55	38818.0
56	34553.0
57	34169.5
58	31655.0
59	29524.0
60	26515.5
61	23507.0
62	19309.0
63	15111.0
64	13805.5
65	9624.5
66	6749.0
67	5708.5
68	4668.0
69	4130.0
70	2237.5
71	883.0
72	551.5
73	220.0
74	418.0
75	336.5
76	57.0
77	40.0
78	23.0
79	16.0
80	9.0
81	8.0
82	3.5
83	0.0
84	0.0
85	0.0
86	1.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	1149368.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.526875023376945
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.61007685686936	50.49738086679848
2	3.512049751653507	3.8300219576858563
3	1.0932122700870788	1.7882834647518098
4	0.5688238716511542	1.2406475263934356
5	0.33108743411355546	0.9026581570860193
6	0.23268538074139697	0.7612564005271819
7	0.17802437606354754	0.6794979033312201
8	0.14335321060573994	0.6253282079119212
9	0.12298010625869579	0.603514879589657
>10	1.018067745365392	11.045379306251437
>50	0.06858544151685214	2.5431364185257745
>100	0.0909511421752127	11.219299188649376
>500	0.022697541276551755	8.554628882785252
>1k	0.007404871622137453	5.7089668397126605
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCGTGGATGGGATTCGCCAGCACCGCCACCACGATGATGACGCCGCGTACCACCTGCT	2923	0.25431367499356167	No Hit
TAGTTGGATGGGGGGTGAAACCCCGTCTCTACTAAAAATACAAAACATTAGCCAGGTGT	2218	0.19297561790479636	No Hit
GGGATGGATGGGGACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCC	2044	0.17783686338927132	No Hit
GACGTGGATGGGGCCTAGGACTAGCCAGCTAGCCTGGCATCACTCATTCCAACAGGGCC	2004	0.17435668993742648	No Hit
ACTGTGGATGGGGGTAGCAAGCAGGGAGGGATCCGCGGCCATCATCAGCGACACCATCA	1979	0.17218158153002344	No Hit
CGATTGGATGGGGGCAGCCTGATACTGCCACATGCTGCACAGTGCCCACCCATCAGAAG	1869	0.16261110453745015	No Hit
GGGATGGATGGGGAATCCCAGCCCTTTGGGAGTCCAAGGCAGGTGGATAGCCTGAGGTC	1791	0.1558247663063527	No Hit
AGGCTGGATGGGGTACTACTTCATTGCAACTTTCATCGCTGACCACATCAGACACCATG	1677	0.14590627196859493	No Hit
TTTTTGGATGGGGCAGGCAGGAGGATCTCTTAGTAGCTTAGTAGAGGTCAGCCTGGTCT	1662	0.1446012069241531	No Hit
CGTTTGGATGGGGAGTTCCTAAGGGCGCATGGTGGATGCCTTGGCAACAGGAGCCGATG	1659	0.14434019391526473	No Hit
GGTCTGGATGGGGGCGCCCCAGTTCCCCTGACCATCAACCAGCGGATAACGGTAAGAGA	1642	0.14286112019823066	No Hit
TAAGTGGATGGGGTTACCCTACTCCACTATGCCCTCATCTTAACTAATTATATCTGCAA	1581	0.1375538556841673	No Hit
GTGGTGGATGGGGAACTGCCAACGTCACTGAGGGCCCTGCTCTGAAGCCCTGGGCCCTG	1542	0.1341606865686186	No Hit
TAGATGGATGTGGGGCCAATATGGTGAAAACCAGACTCTACAAAAAGCACAAAAATTCG	1520	0.13224659117010393	No Hit
CTTTTGGATGGGGTTCCCAGTCTCTGTTTCTTAGCCCAGGAAAAGAATTCCTAGGCTCT	1499	0.1304195001078854	No Hit
TTTGTGGATGGGAGCACACAAGTGTTCATAGTAGTTTCACTTGTAGTAGCCAAAAACAG	1448	0.1259822789567832	No Hit
ATGCTGGATGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCT	1433	0.12467721391234139	No Hit
AGTGTGGATGGGGTCAAACTCTCAATTAACCATGGAAGACATGCACTGCATTATATACT	1416	0.12319814019530734	No Hit
ACTCTGGATGGGGTCACAGGGGCATGTGTTCCACTATGTTCATAATGGCCTTATTTGTG	1385	0.1205010057701276	No Hit
TGGGTGGATGGGAGTTTCTGATATTCCCTGACACAATTAAGGAATGCCTTTAGGCCCTA	1367	0.11893492771679741	No Hit
GTACTGGATGGGGGTACCAACTCCACAGCCCACACCTTCTAATAGTGCCACTTCCTGAG	1363	0.11858691037161292	No Hit
TGATTGGATGGGGGAGGCCAAGATGTTCAGATCGCTTGAGCTCAGGAGTTTGAGACCAG	1340	0.11658581063680215	No Hit
TAACTGGATGGGGGCTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCCTCTCGCAA	1334	0.11606378461902542	No Hit
ATGCTGGATGGGGGCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCC	1307	0.11371466753903016	No Hit
GTCTTGGATGGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	1305	0.1135406588664379	No Hit
GCTATGGATGGGGCTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAGT	1280	0.11136555045903487	No Hit
TATTTGGATGGGGGGAGTCACCCCTCAAGGATGGCCTCTGGGGTTTCTGCTCATGCCCC	1261	0.10971246806940858	No Hit
TCCATGGATGGGGGTTCAAGAGATTCTCGTGCCTCAGCCTCCCAAGTAGCTGGGACTAC	1224	0.10649330762645211	No Hit
TTCGTGGATGGGGTGACCAATGGAAAGAACTTAGTGCTGGTGTGTACCACTGATATACT	1205	0.10484022523682579	No Hit
TTTCTGGATGGGGTTGATGAACTTGGAGTTCCCGTGTTCCTTTAATTAGCCTATGATTT	1189	0.10344815585608787	No Hit
CTTCTGGATGGGGGTCAAGAAAGTCAATGAGGAAATTAAATGTAAATTCTGAGCAGCCA	1188	0.10336115151979175	No Hit
CAAGTGGATGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAACACCGACTGCTCTTCC	1185	0.10310013851090338	No Hit
TCTGTGGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1172	0.1019690821390538	No Hit
TATCTGGATGGGGACAGCAAGTTGTTGTTCTAGAACTTTGAACACTAAATGGAAGAAAA	1157	0.100664017094612	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.022360114428103095	0.0	0.0	0.0	0.0
12	0.08056601541020804	0.0	0.0	0.0	0.0
13	0.19567275232997614	0.0	0.0	0.0	0.0
14	0.6506184268223928	0.0	0.0	0.0	0.0
15	1.0587557683874964	0.0	0.0	0.0	0.0
16	1.245380069742676	0.0	0.0	0.0	0.0
17	1.366925127548357	0.0	0.0	0.0	0.0
18	1.5451100082828129	0.0	0.0	0.0	0.0
19	1.6886671631714125	0.0	0.0	0.0	0.0
20	1.8364005262022258	0.0	0.0	0.0	0.0
21	1.9949224269337584	0.0	0.0	0.0	0.0
22	2.1550104057186212	0.0	0.0	0.0	0.0
23	2.3210146793716198	0.0	0.0	0.0	0.0
24	2.5011136555045903	0.0	0.0	0.0	0.0
25	2.6893040349131	0.0	0.0	0.0	0.0
26	2.8978534290149023	0.0	0.0	0.0	0.0
27	3.0896979905478488	0.0	0.0	0.0	0.0
28	3.2764092962393248	0.0	0.0	0.0	0.0
29	3.5072318004329337	0.0	0.0	0.0	0.0
30	3.76772278330352	0.0	0.0	0.0	0.0
31	3.9834935373179	0.0	0.0	0.0	0.0
32	4.163592513450871	0.0	0.0	0.0	0.0
33	4.309063763737985	0.0	0.0	0.0	0.0
34	4.460277300220643	0.0	0.0	0.0	0.0
35	4.591305830682601	0.0	0.0	0.0	0.0
36	4.719898239728268	0.0	0.0	0.0	0.0
37	4.84814263142875	0.0	0.0	0.0	0.0
38	4.977953101182563	0.0	0.0	0.0	0.0
39	5.115767969875619	0.0	0.0	0.0	0.0
40	5.252712795205713	0.0	0.0	0.0	0.0
41	5.396356954430609	0.0	0.0	0.0	0.0
42	5.542959261089573	0.0	0.0	0.0	0.0
43	5.691997689164828	0.0	0.0	0.0	0.0
44	5.837729952460831	0.0	0.0	0.0	0.0
45	5.97276068239241	0.0	0.0	0.0	0.0
46	6.105442295243995	0.0	0.0	0.0	0.0
47	6.235687786679288	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACG	75	0.0	53.000004	49
TTGGCTT	75	0.0	53.000004	39
TCTGATA	175	0.0	53.000004	17
GTTTCAC	150	0.0	53.000004	34
TCCTCAG	75	0.0	53.000004	52
CGACACG	75	0.0	53.000004	48
TAACGAA	75	0.0	53.000004	51
GCGCACT	155	0.0	53.000004	32
CATTAGC	310	0.0	53.000004	46
CACTACA	155	0.0	53.000004	35
TCTAAAT	135	0.0	53.000004	46
ACTACAC	155	0.0	53.000004	36
TTAACGA	75	0.0	53.000004	50
ACGTCAC	150	0.0	53.000004	22
CGCACTA	155	0.0	53.000004	33
TTTGAGG	75	0.0	53.000004	28
CGCAATT	155	0.0	53.000004	20
GCTAACT	150	0.0	53.000004	38
TATACAA	75	0.0	53.000004	41
ATTTGAC	75	0.0	53.000004	15
>>END_MODULE