FastQCFastQC Report
Fri 17 Jun 2016
SRR1528188_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528188_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences828607
Sequences flagged as poor quality0
Sequence length52
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGTTCATAGGGATCATCACTAATGGAACGCCGGAGGTGAACACCACGCGG30860.37243228695871505No Hit
TTATTTCATAGGGACCAAAACCTGGCAGAGATACAATAAAAAAAGAAAACTT24020.2898841066995572No Hit
GCGATTCATAGGGTGGGCATGGTGGCTCACGCCTGTAATCCCAGCACTTTGG22180.2676781634719475No Hit
ATTTTTCATAGGGGGATCACTTGAGTCCAGGAGTTGGAGACTAGCCTGGACA21430.2586268279172153No Hit
CATGTTCATAGGGGGCCGCTGCGGGGTTGTAGGACCACGAGATCTGATTGAA20580.2483686476218521No Hit
TGGCTTCATAGGGGTACTTTACACTCAAAGACGACACCGCCCAGGTACGCTG20190.24366195313339137No Hit
GGTTTTCATAGGGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTC17430.21035303829197677No Hit
AGGGTTCATAGGGGAGGATAACTGATGCATCACCATCATCACCATGCTCCTG16930.20431881458882195No Hit
GCTCTTCATAGGGTAAACAATACATTTAGGCCAGGCGCAGTGGCTCACGCCT15600.18826777953843016No Hit
TGGATTCATAGGGAGGCCAACATAGTGAAACCGCATCTCTACAAAAATTAGC15290.18452656084247418No Hit
GGTTTTCATAGGGGGGGATGAGGCTGGGAAAGGAGAAGAGATGGACCCGCGA14760.17813028371713008No Hit
TGATTTCATAGGGACGCAGAGTCATATTCATAGGGCGCCTGTAATCCCAACA14200.1713719531695967No Hit
CGTATTCATAGGACGCAGAGTGCAGTTCATAGGGCGGCTGTGGTGGCTCACG14000.16895826368833475No Hit
GCTTTTCATAGGGCACCTGGCAGGAGGGCCAGTGCCATGGGCTTTAACACAT13980.16871689474020857No Hit
GTATTTCATAGGGTGGAGCAGGTCCCAAGGGTTGGGCTGTTCGCCCATTAAA13160.1588207678670347No Hit
GCGGTTCATAGGGTGATGTCACTGTGGTCAGGAGAATGGGATATAAGCTGAC12990.15676913180796204No Hit
AGACTTCATAGGGGTCGCCTGCTGGGTCTGTTCCAGAACCGTTACGGACCTA12990.15676913180796204No Hit
ATAGTTCATAGGGGCTCAGATTGGACGCTGGCGGCAGGCTTAACACATGCAA12950.15628639391170965No Hit
ACCATTCATAGGGGTCTTCCAGAAAACAGAAGACAGAATGCCTCATACCTCG12760.1539933889045108No Hit
AGTGTTCATAGGGGCAGAGTATTATTCATAGGGGTGGACTCTCTGACATAAA12340.1489246409938608No Hit
CCTCTTCATAGGGATCAACGCAGAGTGTTCTTCATAGGGAACTGCTCTGTCC12290.1483212186235453No Hit
ATCCTTCATAGGGATCCGTTGAGCGATGGCCCTTCCATTCAGAACCACCGGA11980.14457999992758933No Hit
GGTTTTCATAGGGTCACCTCCTTACCTTAAAGAAGCGTACTTTGCAGTGCTC11880.14337315518695837No Hit
TCGATTCATAGGGACGTCAGGCAAGTAAGTTACGCGTTGTTTTTGCGACAGA11760.1419249414982012No Hit
TTCCTTCATAGGGGTTGTCCGACCAATAAGTCTGCAAGAAGGCCTGGGTGGC11740.14168357255007502No Hit
TTGATTCATAGGGACAGACAAAGGCTGGTACAGAAATCTAAGAAACACTGTC11550.13939056754287618No Hit
GGCCTTCATAGGGAGTCTCTGTGACCTTATCGACGCGCATGTGAGCCTATAG11300.13637345569129877No Hit
TGGGTTCATAGGGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAA11180.1349252420025416No Hit
TTCGTTCATAGGGGCCTTTTCCTTCAAAAACTATTCCATAAAATTAAAAAAA11120.13420113515816304No Hit
TGTTTTCATAGGGTCTGTGGACTTAGGATGCACAGACTAGCTCCATGGGACT10890.1314253922547118No Hit
GTAGTTCATAGGGGCTGATCAGCTTCGAGTCGGAAGTGAACAACATCCGCAA10700.129132387247513No Hit
GCAATTCATAGGGGTAACACTTTGGGAGGCCGAGGCGGGCGGATCACAAGGT10590.12780485803281894No Hit
TAGTTTCATAGGTGTTTAAAATATCACTTTGAGGACAAGAGTTGATCATGCT10580.12768417355875583No Hit
TTAATTCATAGGGAACCATAAAACTCCTAAAAGAAAACATAGATGGTATGCT10220.12333953249248437No Hit
GCTGTTCATAGGGATCCACAGTGCGCACGCAAAAACGCTGGATACCCAAGGG10170.1227361101221689No Hit
TAGGTTCATAGGGGCTGAGCCACTGTGGTGGGAGCCTGTGCCCAGGTTCAAG10120.12213268775185342No Hit
TTGCTTCATAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9990.12056378958903316No Hit
TATCTTCATAGGGGGTTTCATTTGATGCTCGATGAGTTTTTCTAAGAATTAA9800.11827078458183433No Hit
AGACTTCATAGGGGGTGCGGGCGGGCGCGACCCGCTCCGGGGACAGTGCCAG9790.11815010010777123No Hit
TGTTTTCATAGGGGCCTGTTCCCTCTGGTATCTAATAAGCTAGCAGATTCCC9140.11030560929366998No Hit
CTCATTCATAGGCTGCTTTAAGACCTGATAGTGCCTGCTATATCGAATCACA9120.11006424034554378No Hit
CTTTTTCATAGGGTAATTTTTTCTTCAGGCAAGGGAAACAAGCATAAATAAA8900.10740918191615566No Hit
GGCGTTCATAGGGGAGAACATAGGCAAAACACTCTCCGACATATATCAATCA8900.10740918191615566No Hit
CGTTTTCATAGGGATTACTCCTCATATGCACGATTAGGGCAAGTCGGCCAAG8840.1066850750717771No Hit
TCTATTCATAGGGGCCAAATCAGTAAATTTGTGCTACCATGTATTGCGGGAT8770.10584028375333543No Hit
GGTCTTCATAGGGACACCAAAATAACGATCATCACGACATAATTTTTCTTTA8770.10584028375333543No Hit
CTTGTTCATAGGGTCGAGACCAGCCTGGCCGACATGGAGAAACCCCGTCTCT8550.1031852253239473No Hit
TATGTTCATAGGGACGCAGAGTGTTATTCATAGGGGAGGTTCATAGGGGGAA8360.10089222031674848No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTG1450.046.00000415
CCTTAAA1450.046.00000425
ACGCCTG2950.046.00000429
TACGGAC1450.046.00000443
TAGCCTG2250.046.00000442
TTGAGTC2250.046.00000421
CGTTACG1450.046.00000440
GTTACGG1450.046.00000441
CTTGAGT2250.046.00000420
TACCTCG1450.046.00000446
CGTACTT1450.046.00000436
ACCGTTA1450.046.00000438
CCTGGAC2250.046.00000445
CCGTTAC1450.046.00000439
GCGTACT1450.046.00000435
CTAGCCT2250.046.00000441
TCACGAC750.046.032
CCTTCGA1000.046.021
TATTTTA253.4163248E-546.023
ATTTATA501.6370905E-1146.043
Produced by FastQC (version 0.11.5)