##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1528188_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 828607 Sequences flagged as poor quality 0 Sequence length 52 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.86039461409329 31.0 31.0 33.0 28.0 34.0 2 31.608517668810425 31.0 31.0 34.0 30.0 34.0 3 31.7771742213136 31.0 31.0 34.0 30.0 34.0 4 35.340071952083434 37.0 35.0 37.0 33.0 37.0 5 35.1191964344979 35.0 35.0 37.0 32.0 37.0 6 35.54855317418269 37.0 35.0 37.0 33.0 37.0 7 35.528033193057745 37.0 35.0 37.0 33.0 37.0 8 35.51749140424833 37.0 35.0 37.0 33.0 37.0 9 37.414966322997515 39.0 37.0 39.0 34.0 39.0 10 36.94701227481786 39.0 37.0 39.0 33.0 39.0 11 37.64038681787627 39.0 37.0 39.0 35.0 39.0 12 37.62616777314215 39.0 37.0 39.0 35.0 39.0 13 37.53798604163373 39.0 37.0 39.0 35.0 39.0 14 37.67320092637402 40.0 37.0 40.0 32.0 40.0 15 36.82358343581456 39.0 36.0 40.0 31.0 40.0 16 36.5182686122613 38.0 35.0 40.0 31.0 40.0 17 36.234720440450054 38.0 35.0 40.0 31.0 40.0 18 36.28712043224351 38.0 35.0 40.0 30.0 40.0 19 36.59261507566313 39.0 35.0 40.0 31.0 40.0 20 36.77523844234963 39.0 35.0 40.0 31.0 40.0 21 36.869728351317335 39.0 35.0 40.0 32.0 40.0 22 36.89984395497503 39.0 35.0 40.0 32.0 40.0 23 36.824973720955775 39.0 35.0 40.0 32.0 40.0 24 36.855050705581775 39.0 35.0 40.0 32.0 40.0 25 37.008765313351205 39.0 35.0 40.0 32.0 40.0 26 36.805319047509855 39.0 35.0 40.0 31.0 40.0 27 36.60067317799633 39.0 35.0 40.0 31.0 40.0 28 36.47664574400168 39.0 35.0 40.0 31.0 40.0 29 36.36311303187156 38.0 35.0 40.0 31.0 40.0 30 36.22881776282363 38.0 35.0 40.0 30.0 40.0 31 36.139195058694895 38.0 35.0 40.0 30.0 40.0 32 36.096634472071806 38.0 35.0 40.0 30.0 40.0 33 35.942254892850286 38.0 35.0 40.0 30.0 40.0 34 35.69831295173707 38.0 35.0 40.0 30.0 40.0 35 35.59056585329354 38.0 35.0 40.0 30.0 40.0 36 35.381591031695365 38.0 35.0 40.0 29.0 40.0 37 35.163412812105136 37.0 34.0 40.0 27.0 40.0 38 34.848282720276316 37.0 34.0 40.0 26.0 40.0 39 34.422332903294325 36.0 33.0 40.0 24.0 40.0 40 33.94596714727247 35.0 33.0 40.0 23.0 40.0 41 33.474719619795636 35.0 33.0 40.0 21.0 40.0 42 32.99463919566212 35.0 33.0 40.0 18.0 40.0 43 32.492448169035505 35.0 33.0 40.0 15.0 40.0 44 31.953834568136642 35.0 31.0 39.0 12.0 40.0 45 31.543257539460807 35.0 31.0 39.0 10.0 40.0 46 31.062432492122323 35.0 29.0 39.0 9.0 40.0 47 30.505706565356075 35.0 26.0 39.0 9.0 40.0 48 30.083656063731055 35.0 24.0 38.0 8.0 40.0 49 29.76303965571133 35.0 24.0 38.0 8.0 40.0 50 29.33271744023403 35.0 23.0 38.0 8.0 40.0 51 28.82664882145577 35.0 20.0 38.0 8.0 40.0 52 27.822882259020258 34.0 17.0 37.0 8.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 2.0 13 0.0 14 2.0 15 15.0 16 57.0 17 160.0 18 406.0 19 879.0 20 1579.0 21 2571.0 22 3695.0 23 5430.0 24 7249.0 25 10033.0 26 13695.0 27 17964.0 28 23395.0 29 29932.0 30 36680.0 31 39928.0 32 41341.0 33 46120.0 34 58675.0 35 79234.0 36 90379.0 37 126835.0 38 152553.0 39 39798.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.94962871421555 20.60868421338463 38.60189450487384 6.83979256752598 2 28.061553909151144 20.886741241626005 33.96435222005124 17.08735262917161 3 24.542032592049065 23.435718018312663 34.507915091231425 17.514334298406844 4 25.329257416362644 21.94755776864062 33.50104452412302 19.222140290873718 5 0.0 0.0 100.0 0.0 6 0.0 0.0 100.0 0.0 7 0.0 0.0 0.0 100.0 8 0.0 100.0 0.0 0.0 9 0.0 0.0 100.0 0.0 10 0.0 100.0 0.0 0.0 11 99.49203904866842 0.3905349580681795 0.09703031714672938 0.020395676116663267 12 98.55661369020537 1.148433455184424 0.19152626033813375 0.1034265942720735 13 95.3620956617552 2.615473921895422 1.105711151366088 0.9167192649832792 14 51.50403025801133 27.16969564582486 12.642543449427773 8.683730646736027 15 33.1295777129568 31.562489817247503 23.767117584089924 11.54081488570577 16 37.97421455527168 24.965273042588343 22.03481264338824 15.025699758751736 17 25.437873443019427 28.959205027232453 20.93561845362156 24.667303076126558 18 19.133195833489218 39.07256395371992 24.036243961250626 17.757996251540238 19 19.696188904993562 39.23271225080165 27.343601972949784 13.72749687125501 20 14.96704710435707 39.202782501234005 29.45099425903957 16.37917613536936 21 22.109757701781422 37.5611116005537 18.063086601971744 22.266044095693132 22 22.406762192450703 38.35750844489607 20.867069672353722 18.368659690299502 23 16.885930241960303 39.96309468783151 26.700353726193477 16.450621344014714 24 17.0978521784151 39.967439328897775 19.850665031794325 23.0840434608928 25 21.513817768857855 41.38922311783511 20.390969422174805 16.705989691132224 26 16.67678404840896 40.333354654257086 22.281974446269462 20.707886851064497 27 17.687395834213323 46.804576837994375 17.94324691922709 17.56478040856522 28 16.242923364152126 46.62222259768503 17.368064715842372 19.76678932232047 29 16.523876819771015 50.406646335355596 17.140212428811246 15.92926441606214 30 20.346557535719587 52.478316017122715 15.695619274275984 11.479507172881716 31 18.56308237801515 52.49062583347715 14.868085835625333 14.078205952882367 32 17.26620701973312 55.49047980526354 15.684757671610306 11.558555503393043 33 17.752806819155523 53.45899805335943 16.023881043727606 12.764314083757439 34 16.273456536090087 54.26360144193809 16.29686932405833 13.166072697913487 35 17.364685550568602 54.47962665051104 16.053810793295252 12.101877005625102 36 15.5294367534911 53.66717877111827 19.46302650110366 11.340357974286967 37 15.983210375968342 53.82624090793343 18.08601665204373 12.104532064054492 38 14.756452697116968 55.048171207822286 18.06646576724551 12.128910327815237 39 16.106067170564575 51.74733015772254 21.162022526963927 10.984580144748959 40 16.52846282978541 50.7425112266732 20.08394812015829 12.6450778233831 41 15.627915284326587 52.53250334597704 19.94564371288198 11.893937656814387 42 16.08796449945511 49.27981540102847 18.92948044127071 15.702739658245706 43 16.268629157127563 50.81045658557072 17.52290289606532 15.398011361236389 44 18.230475967497256 51.13932177739267 17.887249323261813 12.74295293184827 45 17.890025066165265 48.30673648665773 19.897128554308615 13.906109892868393 46 21.014908153081013 47.79298268057113 17.883749473513983 13.308359692833877 47 21.616882309707737 47.67965995942588 16.488516268870526 14.214941461995856 48 21.61808915444837 45.77815538608774 18.14720368039372 14.456551779070173 49 21.89795644980069 46.29721930903311 17.108472412132652 14.696351829033546 50 21.1212311747306 46.90884822358488 16.661939858099196 15.307980743585317 51 20.58140952224637 46.961587338750455 17.780926301612222 14.676076837390948 52 23.115059370727014 46.176655519444076 15.718911377770162 14.989373732058745 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 3.0 2 6.0 3 6.0 4 6.0 5 66.5 6 127.0 7 702.5 8 1278.0 9 2012.5 10 2747.0 11 5180.5 12 7614.0 13 7600.5 14 8266.5 15 8946.0 16 9554.5 17 10163.0 18 12534.0 19 14905.0 20 19657.5 21 24410.0 22 32418.0 23 40426.0 24 49228.0 25 58030.0 26 57444.0 27 56858.0 28 50995.0 29 45132.0 30 41557.5 31 37983.0 32 34010.5 33 30038.0 34 27288.5 35 24539.0 36 25693.0 37 26847.0 38 26461.5 39 32411.0 40 38746.0 41 42845.0 42 46944.0 43 50155.5 44 53367.0 45 56120.5 46 58874.0 47 57179.5 48 55485.0 49 51442.0 50 47399.0 51 41694.0 52 35989.0 53 31876.5 54 27764.0 55 21335.0 56 14906.0 57 13088.0 58 11270.0 59 8751.5 60 6233.0 61 4646.5 62 3060.0 63 2333.5 64 1411.5 65 1216.0 66 743.5 67 271.0 68 340.5 69 410.0 70 273.0 71 136.0 72 613.0 73 1090.0 74 567.0 75 44.0 76 29.0 77 14.0 78 10.0 79 6.0 80 8.5 81 11.0 82 24.5 83 38.0 84 22.5 85 7.0 86 4.0 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 52 828607.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.64777154835187 #Duplication Level Percentage of deduplicated Percentage of total 1 84.99085912947416 32.847073073340724 2 6.555679327518718 5.067247939883928 3 2.199910959959062 2.5506496862163983 4 1.1218675636653934 1.7343072523218488 5 0.7153495657025708 1.3823333296242837 6 0.5460106521182225 1.2661256967619015 7 0.3921688435780074 1.060951631248892 8 0.3235577351770911 1.0003828345461077 9 0.2612323715481629 0.9086444114964818 >10 2.4183169128015787 19.585037662886588 >50 0.2624008486839908 7.013600634918947 >100 0.18000443962913962 13.61567356602471 >500 0.0212329180548456 5.639393293699988 >1k 0.011408732089170771 6.328578987029192 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAAGTTCATAGGGATCATCACTAATGGAACGCCGGAGGTGAACACCACGCGG 3086 0.37243228695871505 No Hit TTATTTCATAGGGACCAAAACCTGGCAGAGATACAATAAAAAAAGAAAACTT 2402 0.2898841066995572 No Hit GCGATTCATAGGGTGGGCATGGTGGCTCACGCCTGTAATCCCAGCACTTTGG 2218 0.2676781634719475 No Hit ATTTTTCATAGGGGGATCACTTGAGTCCAGGAGTTGGAGACTAGCCTGGACA 2143 0.2586268279172153 No Hit CATGTTCATAGGGGGCCGCTGCGGGGTTGTAGGACCACGAGATCTGATTGAA 2058 0.2483686476218521 No Hit TGGCTTCATAGGGGTACTTTACACTCAAAGACGACACCGCCCAGGTACGCTG 2019 0.24366195313339137 No Hit GGTTTTCATAGGGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTC 1743 0.21035303829197677 No Hit AGGGTTCATAGGGGAGGATAACTGATGCATCACCATCATCACCATGCTCCTG 1693 0.20431881458882195 No Hit GCTCTTCATAGGGTAAACAATACATTTAGGCCAGGCGCAGTGGCTCACGCCT 1560 0.18826777953843016 No Hit TGGATTCATAGGGAGGCCAACATAGTGAAACCGCATCTCTACAAAAATTAGC 1529 0.18452656084247418 No Hit GGTTTTCATAGGGGGGGATGAGGCTGGGAAAGGAGAAGAGATGGACCCGCGA 1476 0.17813028371713008 No Hit TGATTTCATAGGGACGCAGAGTCATATTCATAGGGCGCCTGTAATCCCAACA 1420 0.1713719531695967 No Hit CGTATTCATAGGACGCAGAGTGCAGTTCATAGGGCGGCTGTGGTGGCTCACG 1400 0.16895826368833475 No Hit GCTTTTCATAGGGCACCTGGCAGGAGGGCCAGTGCCATGGGCTTTAACACAT 1398 0.16871689474020857 No Hit GTATTTCATAGGGTGGAGCAGGTCCCAAGGGTTGGGCTGTTCGCCCATTAAA 1316 0.1588207678670347 No Hit GCGGTTCATAGGGTGATGTCACTGTGGTCAGGAGAATGGGATATAAGCTGAC 1299 0.15676913180796204 No Hit AGACTTCATAGGGGTCGCCTGCTGGGTCTGTTCCAGAACCGTTACGGACCTA 1299 0.15676913180796204 No Hit ATAGTTCATAGGGGCTCAGATTGGACGCTGGCGGCAGGCTTAACACATGCAA 1295 0.15628639391170965 No Hit ACCATTCATAGGGGTCTTCCAGAAAACAGAAGACAGAATGCCTCATACCTCG 1276 0.1539933889045108 No Hit AGTGTTCATAGGGGCAGAGTATTATTCATAGGGGTGGACTCTCTGACATAAA 1234 0.1489246409938608 No Hit CCTCTTCATAGGGATCAACGCAGAGTGTTCTTCATAGGGAACTGCTCTGTCC 1229 0.1483212186235453 No Hit ATCCTTCATAGGGATCCGTTGAGCGATGGCCCTTCCATTCAGAACCACCGGA 1198 0.14457999992758933 No Hit GGTTTTCATAGGGTCACCTCCTTACCTTAAAGAAGCGTACTTTGCAGTGCTC 1188 0.14337315518695837 No Hit TCGATTCATAGGGACGTCAGGCAAGTAAGTTACGCGTTGTTTTTGCGACAGA 1176 0.1419249414982012 No Hit TTCCTTCATAGGGGTTGTCCGACCAATAAGTCTGCAAGAAGGCCTGGGTGGC 1174 0.14168357255007502 No Hit TTGATTCATAGGGACAGACAAAGGCTGGTACAGAAATCTAAGAAACACTGTC 1155 0.13939056754287618 No Hit GGCCTTCATAGGGAGTCTCTGTGACCTTATCGACGCGCATGTGAGCCTATAG 1130 0.13637345569129877 No Hit TGGGTTCATAGGGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAA 1118 0.1349252420025416 No Hit TTCGTTCATAGGGGCCTTTTCCTTCAAAAACTATTCCATAAAATTAAAAAAA 1112 0.13420113515816304 No Hit TGTTTTCATAGGGTCTGTGGACTTAGGATGCACAGACTAGCTCCATGGGACT 1089 0.1314253922547118 No Hit GTAGTTCATAGGGGCTGATCAGCTTCGAGTCGGAAGTGAACAACATCCGCAA 1070 0.129132387247513 No Hit GCAATTCATAGGGGTAACACTTTGGGAGGCCGAGGCGGGCGGATCACAAGGT 1059 0.12780485803281894 No Hit TAGTTTCATAGGTGTTTAAAATATCACTTTGAGGACAAGAGTTGATCATGCT 1058 0.12768417355875583 No Hit TTAATTCATAGGGAACCATAAAACTCCTAAAAGAAAACATAGATGGTATGCT 1022 0.12333953249248437 No Hit GCTGTTCATAGGGATCCACAGTGCGCACGCAAAAACGCTGGATACCCAAGGG 1017 0.1227361101221689 No Hit TAGGTTCATAGGGGCTGAGCCACTGTGGTGGGAGCCTGTGCCCAGGTTCAAG 1012 0.12213268775185342 No Hit TTGCTTCATAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 999 0.12056378958903316 No Hit TATCTTCATAGGGGGTTTCATTTGATGCTCGATGAGTTTTTCTAAGAATTAA 980 0.11827078458183433 No Hit AGACTTCATAGGGGGTGCGGGCGGGCGCGACCCGCTCCGGGGACAGTGCCAG 979 0.11815010010777123 No Hit TGTTTTCATAGGGGCCTGTTCCCTCTGGTATCTAATAAGCTAGCAGATTCCC 914 0.11030560929366998 No Hit CTCATTCATAGGCTGCTTTAAGACCTGATAGTGCCTGCTATATCGAATCACA 912 0.11006424034554378 No Hit CTTTTTCATAGGGTAATTTTTTCTTCAGGCAAGGGAAACAAGCATAAATAAA 890 0.10740918191615566 No Hit GGCGTTCATAGGGGAGAACATAGGCAAAACACTCTCCGACATATATCAATCA 890 0.10740918191615566 No Hit CGTTTTCATAGGGATTACTCCTCATATGCACGATTAGGGCAAGTCGGCCAAG 884 0.1066850750717771 No Hit TCTATTCATAGGGGCCAAATCAGTAAATTTGTGCTACCATGTATTGCGGGAT 877 0.10584028375333543 No Hit GGTCTTCATAGGGACACCAAAATAACGATCATCACGACATAATTTTTCTTTA 877 0.10584028375333543 No Hit CTTGTTCATAGGGTCGAGACCAGCCTGGCCGACATGGAGAAACCCCGTCTCT 855 0.1031852253239473 No Hit TATGTTCATAGGGACGCAGAGTGTTATTCATAGGGGAGGTTCATAGGGGGAA 836 0.10089222031674848 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.2615232552947296 0.0 0.0 0.0 0.0 11 0.27962592640419404 0.0 0.0 0.0 0.0 12 0.3800354088246901 0.0 0.0 0.0 0.0 13 0.48491021678552076 0.0 0.0 0.0 0.0 14 0.7620017692343898 0.0 0.0 0.0 0.0 15 1.1907937055805708 0.0 0.0 0.0 0.0 16 1.4003019525541058 0.0 0.0 0.0 0.0 17 1.5476576953851464 0.0 0.0 0.0 0.0 18 1.7438906502117408 0.0 0.0 0.0 0.0 19 1.9314343229057924 0.0 0.0 0.0 0.0 20 2.118977995599844 0.0 0.0 0.0 0.0 21 2.359381467933532 0.0 0.0 0.0 0.0 22 2.6075087466072575 0.0 0.0 0.0 0.0 23 2.801931434322906 0.0 0.0 0.0 0.0 24 3.0093880452373685 0.0 0.0 0.0 0.0 25 3.273566358961486 0.0 0.0 0.0 0.0 26 3.6038797644721803 0.0 0.0 0.0 0.0 27 3.9018497309339653 0.0 0.0 0.0 0.0 28 4.173510482049995 0.0 0.0 0.0 0.0 29 4.56271791090348 0.0 0.0 0.0 0.0 30 5.05498988060685 0.0 0.0 0.0 0.0 31 5.4303185949430794 0.0 0.0 0.0 0.0 32 5.67434260149866 0.0 0.0 0.0 0.0 33 5.874075406073084 0.0 0.0 0.0 0.0 34 6.081894070409736 0.0 0.0 0.0 0.0 35 6.283316457621043 0.0 0.0 0.0 0.0 36 6.473153135322294 0.0 0.0 0.0 0.0 37 6.671196357259835 0.0 0.0 0.0 0.0 38 6.870808477360196 0.0 0.0 0.0 0.0 39 7.071144704304936 0.0 0.0 0.0 0.0 40 7.261946857798691 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGCCTG 145 0.0 46.000004 15 CCTTAAA 145 0.0 46.000004 25 ACGCCTG 295 0.0 46.000004 29 TACGGAC 145 0.0 46.000004 43 TAGCCTG 225 0.0 46.000004 42 TTGAGTC 225 0.0 46.000004 21 CGTTACG 145 0.0 46.000004 40 GTTACGG 145 0.0 46.000004 41 CTTGAGT 225 0.0 46.000004 20 TACCTCG 145 0.0 46.000004 46 CGTACTT 145 0.0 46.000004 36 ACCGTTA 145 0.0 46.000004 38 CCTGGAC 225 0.0 46.000004 45 CCGTTAC 145 0.0 46.000004 39 GCGTACT 145 0.0 46.000004 35 CTAGCCT 225 0.0 46.000004 41 TCACGAC 75 0.0 46.0 32 CCTTCGA 100 0.0 46.0 21 TATTTTA 25 3.4163248E-5 46.0 23 ATTTATA 50 1.6370905E-11 46.0 43 >>END_MODULE