SRR1528187_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1528187_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	825053
Sequences flagged as poor quality	0
Sequence length	52
%GC	37

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAAGTTCATAGGGATCATCACTAATGGAACGCCGGAGGTGAACACCACGCGG	2984	0.3616737348994549	No Hit
TTATTTCATAGGGACCAAAACCTGGCAGAGATACAATAAAAAAAGAAAACTT	2398	0.2906479947348837	No Hit
GCGATTCATAGGGTGGGCATGGTGGCTCACGCCTGTAATCCCAGCACTTTGG	2160	0.26180136306394863	No Hit
ATTTTTCATAGGGGGATCACTTGAGTCCAGGAGTTGGAGACTAGCCTGGACA	2044	0.24774166023273655	No Hit
CATGTTCATAGGGGGCCGCTGCGGGGTTGTAGGACCACGAGATCTGATTGAA	1998	0.2421662608341525	No Hit
TGGCTTCATAGGGGTACTTTACACTCAAAGACGACACCGCCCAGGTACGCTG	1971	0.23889374379585313	No Hit
GGTTTTCATAGGGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTC	1846	0.2237432019518746	No Hit
AGGGTTCATAGGGGAGGATAACTGATGCATCACCATCATCACCATGCTCCTG	1735	0.2102895207944217	No Hit
GCTCTTCATAGGGTAAACAATACATTTAGGCCAGGCGCAGTGGCTCACGCCT	1591	0.19283609659015843	No Hit
TGGATTCATAGGGAGGCCAACATAGTGAAACCGCATCTCTACAAAAATTAGC	1550	0.18786671886533352	No Hit
GGTTTTCATAGGGGGGGATGAGGCTGGGAAAGGAGAAGAGATGGACCCGCGA	1469	0.17804916775043542	No Hit
CGTATTCATAGGACGCAGAGTGCAGTTCATAGGGCGGCTGTGGTGGCTCACG	1417	0.17174654234334036	No Hit
GCTTTTCATAGGGCACCTGGCAGGAGGGCCAGTGCCATGGGCTTTAACACAT	1351	0.16374705624971972	No Hit
GTATTTCATAGGGTGGAGCAGGTCCCAAGGGTTGGGCTGTTCGCCCATTAAA	1346	0.16314103457596058	No Hit
TGATTTCATAGGGACGCAGAGTCATATTCATAGGGCGCCTGTAATCCCAACA	1339	0.16229260423269778	No Hit
GCGGTTCATAGGGTGATGTCACTGTGGTCAGGAGAATGGGATATAAGCTGAC	1319	0.1598685175376612	No Hit
ATAGTTCATAGGGGCTCAGATTGGACGCTGGCGGCAGGCTTAACACATGCAA	1313	0.15914129152915024	No Hit
AGACTTCATAGGGGTCGCCTGCTGGGTCTGTTCCAGAACCGTTACGGACCTA	1311	0.1588988828596466	No Hit
CCTCTTCATAGGGATCAACGCAGAGTGTTCTTCATAGGGAACTGCTCTGTCC	1224	0.14835410573623756	No Hit
TCGATTCATAGGGACGTCAGGCAAGTAAGTTACGCGTTGTTTTTGCGACAGA	1200	0.1454452017021937	No Hit
GGTTTTCATAGGGTCACCTCCTTACCTTAAAGAAGCGTACTTTGCAGTGCTC	1182	0.14326352367666076	No Hit
TGGGTTCATAGGGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAA	1175	0.14241509333339797	No Hit
ACCATTCATAGGGGTCTTCCAGAAAACAGAAGACAGAATGCCTCATACCTCG	1165	0.1412030499858797	No Hit
TTCCTTCATAGGGGTTGTCCGACCAATAAGTCTGCAAGAAGGCCTGGGTGGC	1158	0.14035461964261692	No Hit
AGTGTTCATAGGGGCAGAGTATTATTCATAGGGGTGGACTCTCTGACATAAA	1150	0.13938498496460228	No Hit
TTGATTCATAGGGACAGACAAAGGCTGGTACAGAAATCTAAGAAACACTGTC	1144	0.1386577589560913	No Hit
ATCCTTCATAGGGATCCGTTGAGCGATGGCCCTTCCATTCAGAACCACCGGA	1133	0.1373245112738212	No Hit
TGTTTTCATAGGGTCTGTGGACTTAGGATGCACAGACTAGCTCCATGGGACT	1122	0.1359912635915511	No Hit
TTCGTTCATAGGGGCCTTTTCCTTCAAAAACTATTCCATAAAATTAAAAAAA	1110	0.13453681157452915	No Hit
GGCCTTCATAGGGAGTCTCTGTGACCTTATCGACGCGCATGTGAGCCTATAG	1102	0.13356717689651454	No Hit
GCTGTTCATAGGGATCCACAGTGCGCACGCAAAAACGCTGGATACCCAAGGG	1100	0.13332476822701086	No Hit
GCAATTCATAGGGGTAACACTTTGGGAGGCCGAGGCGGGCGGATCACAAGGT	1046	0.12677973415041216	No Hit
GTAGTTCATAGGGGCTGATCAGCTTCGAGTCGGAAGTGAACAACATCCGCAA	1035	0.12544648646814205	No Hit
AGACTTCATAGGGGGTGCGGGCGGGCGCGACCCGCTCCGGGGACAGTGCCAG	990	0.1199922914043098	No Hit
TAGTTTCATAGGTGTTTAAAATATCACTTTGAGGACAAGAGTTGATCATGCT	989	0.11987108706955796	No Hit
TATCTTCATAGGGGGTTTCATTTGATGCTCGATGAGTTTTTCTAAGAATTAA	976	0.11829543071778419	No Hit
TAGGTTCATAGGGGCTGAGCCACTGTGGTGGGAGCCTGTGCCCAGGTTCAAG	965	0.11696218303551408	No Hit
TTAATTCATAGGGAACCATAAAACTCCTAAAAGAAAACATAGATGGTATGCT	958	0.11611375269225128	No Hit
TTGCTTCATAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	950	0.11514411801423666	No Hit
CGTTTTCATAGGGATTACTCCTCATATGCACGATTAGGGCAAGTCGGCCAAG	932	0.11296243998870376	No Hit
CTCATTCATAGGCTGCTTTAAGACCTGATAGTGCCTGCTATATCGAATCACA	922	0.11175039664118548	No Hit
TCTATTCATAGGGGCCAAATCAGTAAATTTGTGCTACCATGTATTGCGGGAT	919	0.11138678363693	No Hit
CTTGTTCATAGGGTCGAGACCAGCCTGGCCGACATGGAGAAACCCCGTCTCT	905	0.10968992295040439	No Hit
CTTTTTCATAGGGTAATTTTTTCTTCAGGCAAGGGAAACAAGCATAAATAAA	870	0.10544777123409042	No Hit
GGCGTTCATAGGGGAGAACATAGGCAAAACACTCTCCGACATATATCAATCA	867	0.10508415822983494	No Hit
TGTTTTCATAGGGGCCTGTTCCCTCTGGTATCTAATAAGCTAGCAGATTCCC	864	0.10472054522557946	No Hit
GTGGTTCATAGGCTCAGGAGACACTGGAGAAGTGTCTCACTCAAAGGCAGAA	850	0.10302368453905385	No Hit
GGTCTTCATAGGGACACCAAAATAACGATCATCACGACATAATTTTTCTTTA	848	0.1027812758695502	No Hit
TTCCTTCATAGGGACAAGATGAATCTCACAGCTATGATGTTGAGCAAAAGAC	848	0.1027812758695502	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCACAC	105	0.0	46.000004	38
GTGTTTG	55	1.8189894E-12	46.000004	46
CTTAATG	55	1.8189894E-12	46.000004	24
CTTAACA	110	0.0	46.000004	39
TAATATA	55	1.8189894E-12	46.000004	36
TCGCATC	55	1.8189894E-12	46.000004	20
TCTGACG	110	0.0	46.000004	29
GCCTTGG	55	1.8189894E-12	46.000004	36
ACTGCGG	55	1.8189894E-12	46.000004	24
CATATCT	55	1.8189894E-12	46.000004	17
ATTAGCT	55	1.8189894E-12	46.000004	45
ATCTACT	55	1.8189894E-12	46.000004	33
TTGTGAG	55	1.8189894E-12	46.000004	29
GTCTGAC	110	0.0	46.000004	28
TTAGCTA	55	1.8189894E-12	46.000004	46
GCGCGTG	55	1.8189894E-12	46.000004	42
GCTATTA	55	1.8189894E-12	46.000004	42
TAGAAAC	55	1.8189894E-12	46.000004	28
TGGCGCT	55	1.8189894E-12	46.000004	46
TTGTCCG	110	0.0	46.000004	15

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content