##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528187_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	825053
Sequences flagged as poor quality	0
Sequence length	52
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.906198753292212	31.0	31.0	33.0	28.0	34.0
2	31.64175149960063	31.0	31.0	34.0	30.0	34.0
3	31.798084486693583	31.0	31.0	34.0	30.0	34.0
4	35.37691639203784	37.0	35.0	37.0	33.0	37.0
5	35.234550992481694	37.0	35.0	37.0	33.0	37.0
6	35.60471751511721	37.0	35.0	37.0	33.0	37.0
7	35.532219142285406	37.0	35.0	37.0	33.0	37.0
8	35.51980781840682	37.0	35.0	37.0	33.0	37.0
9	37.4052382089393	39.0	37.0	39.0	34.0	39.0
10	36.9191712532407	39.0	37.0	39.0	33.0	39.0
11	37.63816869946537	39.0	37.0	39.0	35.0	39.0
12	37.64858499999394	39.0	37.0	39.0	35.0	39.0
13	37.5308058997422	39.0	37.0	39.0	35.0	39.0
14	37.64014433012182	40.0	37.0	40.0	32.0	40.0
15	36.832980426711984	39.0	36.0	40.0	31.0	40.0
16	36.51668559474361	38.0	35.0	40.0	31.0	40.0
17	36.25811917537419	38.0	35.0	40.0	31.0	40.0
18	36.21293298733536	38.0	35.0	40.0	30.0	40.0
19	36.59507086211431	39.0	35.0	40.0	31.0	40.0
20	36.804837992225956	39.0	35.0	40.0	32.0	40.0
21	36.93735917571356	39.0	35.0	40.0	32.0	40.0
22	36.88241482668386	39.0	35.0	40.0	32.0	40.0
23	36.88703998409799	39.0	35.0	40.0	32.0	40.0
24	36.983904064344955	39.0	35.0	40.0	32.0	40.0
25	37.05406804168944	39.0	35.0	40.0	32.0	40.0
26	36.719182888856835	39.0	35.0	40.0	31.0	40.0
27	36.484048903524986	39.0	35.0	40.0	31.0	40.0
28	36.409303402326884	39.0	35.0	40.0	30.0	40.0
29	36.29397505372382	38.0	35.0	40.0	30.0	40.0
30	36.041137963258116	38.0	35.0	40.0	30.0	40.0
31	36.01285493174378	38.0	35.0	40.0	30.0	40.0
32	36.06407103543651	38.0	35.0	40.0	30.0	40.0
33	36.00455364685663	38.0	35.0	40.0	30.0	40.0
34	35.81039642301767	38.0	35.0	40.0	30.0	40.0
35	35.53955685271128	38.0	35.0	40.0	29.0	40.0
36	35.37562920200278	38.0	35.0	40.0	29.0	40.0
37	35.15922492252013	37.0	34.0	40.0	27.0	40.0
38	34.89433042483331	37.0	34.0	40.0	26.0	40.0
39	34.46528404841871	36.0	33.0	40.0	24.0	40.0
40	33.9375361340423	35.0	33.0	40.0	23.0	40.0
41	33.43562292361824	35.0	33.0	40.0	21.0	40.0
42	32.95604524800225	35.0	33.0	40.0	18.0	40.0
43	32.48346954680487	35.0	33.0	39.0	15.0	40.0
44	31.870344086985927	35.0	31.0	39.0	11.0	40.0
45	31.464044128074196	35.0	31.0	39.0	10.0	40.0
46	30.97303203551772	35.0	29.0	39.0	9.0	40.0
47	30.535281975824585	35.0	27.0	38.0	9.0	40.0
48	30.10528172129548	35.0	24.0	38.0	8.0	40.0
49	29.61236066046666	35.0	23.0	38.0	8.0	40.0
50	29.18401969328031	35.0	22.0	38.0	8.0	40.0
51	28.682354951742493	35.0	20.0	38.0	8.0	40.0
52	27.491356312867172	33.0	15.0	36.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	2.0
14	2.0
15	19.0
16	55.0
17	198.0
18	432.0
19	838.0
20	1582.0
21	2487.0
22	3800.0
23	5442.0
24	7430.0
25	10044.0
26	13584.0
27	18035.0
28	23365.0
29	29933.0
30	36498.0
31	40219.0
32	41064.0
33	45840.0
34	58763.0
35	78553.0
36	90738.0
37	127217.0
38	152852.0
39	36060.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.08387097556157	20.569830059402243	38.52128287516074	6.825016089875438
2	28.01759402123258	20.944593862454898	33.92885063141398	17.108961484898547
3	24.336860783489062	23.58224259532418	34.553901385729155	17.5269952354576
4	25.3044349878129	22.031069519170284	33.471304267725834	19.19319122529098
5	0.0	0.0	100.0	0.0
6	0.0	0.0	100.0	0.0
7	0.0	0.0	0.0	100.0
8	0.0	100.0	0.0	0.0
9	0.0	0.0	100.0	0.0
10	0.0	100.0	0.0	0.0
11	99.49833525846218	0.38179365446825836	0.09853912415323622	0.021331962916321737
12	98.61499806679086	1.1126557930217817	0.18035205011072017	0.0919940900766375
13	95.41011304728302	2.6119534139018947	1.0799306226387881	0.8980029161762942
14	51.53087135008296	27.10613742389883	12.684518449117816	8.678472776900394
15	33.126841548361135	31.57663810688525	23.704416564754023	11.592103779999588
16	37.891747560459756	25.00239378561135	22.09700467727528	15.008853976653622
17	25.33461486716611	29.035468024478426	20.977803850176898	24.652113258178566
18	19.10580289993491	39.02015991699927	24.010942327341393	17.86309485572442
19	19.58225713984435	39.33323071366324	27.336062046923047	13.748450099569363
20	15.035640134633773	39.22414681238659	29.351205316506938	16.38900773647269
21	22.07797559671924	37.56473826529932	18.065990912098982	22.29129522588246
22	22.434922362563377	38.3724439520855	20.87659823065912	18.316035454692
23	16.93963902925024	39.857075848460646	26.687012834327007	16.516272287962106
24	17.143383515968065	39.948463916863524	19.775456849438765	23.13269571772965
25	21.606733143204135	41.34958602659465	20.32354285118653	16.72013797901468
26	16.60014568761037	40.32104604189064	22.365472278750577	20.71333599174841
27	17.75958635384636	46.71857444309639	17.962361205886168	17.55947799717109
28	16.212291816404523	46.52840484187076	17.275011423508552	19.98429191821616
29	16.48463795659188	50.48305987615341	17.14568639832835	15.88661576892636
30	20.308513513677305	52.44572166878976	15.762260121470984	11.48350469606195
31	18.592017724921913	52.566077573198335	14.830804808903187	14.011099892976572
32	17.20410688767873	55.53037198822379	15.70614251448089	11.559378609616594
33	17.76819186161374	53.61085893875909	15.880191939184513	12.740757260442662
34	16.27846938317902	54.30584459422607	16.205261964988914	13.210424057605996
35	17.284586565953944	54.410686343786395	16.21726119412935	12.08746589613031
36	15.458400854248152	53.83642020573224	19.37742181411376	11.327757125905851
37	16.054726181227146	53.80902802607832	18.015448704507467	12.12079708818706
38	14.832501669589712	55.10445995590586	18.003570679701788	12.059467694802636
39	16.219927689493886	51.76564414649726	21.0839788474195	10.930449316589359
40	16.521484074356437	50.720377963597485	20.05495404537648	12.703183916669596
41	15.632813891956033	52.53929141521817	19.934719345302664	11.893175347523128
42	16.02648557122997	49.33295194369332	18.92835975385824	15.71220273121848
43	16.31895163098613	50.75637565101878	17.512208306617875	15.412464411377208
44	18.236161798090546	51.16071331175088	17.91739439769324	12.685730492465334
45	17.82127936023504	48.32331983521059	19.942840035731038	13.912560768823337
46	20.970895203096042	47.841290195902566	17.828915233324405	13.358899367676985
47	21.626731858438188	47.817412941956455	16.40040094393936	14.155454255666001
48	21.612672155606973	45.91608054270453	17.99351071991739	14.477736581771111
49	21.81144726459997	46.29726817549903	17.18350215077092	14.707782409130079
50	21.15306531822804	46.91710714341988	16.648385012841597	15.281442525510483
51	20.449110541989423	47.08158142567811	17.82661235096412	14.642695681368348
52	23.25014271810417	46.03449717775707	15.7534122050341	14.961947899104663
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	3.5
4	6.0
5	72.0
6	138.0
7	701.5
8	1265.0
9	2004.0
10	2743.0
11	5112.5
12	7482.0
13	7399.5
14	8177.5
15	9038.0
16	9439.5
17	9841.0
18	12214.0
19	14587.0
20	19359.5
21	24132.0
22	32221.0
23	40310.0
24	49315.5
25	58321.0
26	57640.0
27	56959.0
28	51139.0
29	45319.0
30	41533.5
31	37748.0
32	33810.0
33	29872.0
34	27060.5
35	24249.0
36	25573.5
37	26898.0
38	26503.5
39	32239.5
40	38370.0
41	42671.5
42	46973.0
43	49942.0
44	52911.0
45	55705.0
46	58499.0
47	57010.5
48	55522.0
49	51346.5
50	47171.0
51	41476.5
52	35782.0
53	31592.5
54	27403.0
55	21120.5
56	14838.0
57	13053.5
58	11269.0
59	8726.5
60	6184.0
61	4634.0
62	3084.0
63	2295.5
64	1341.0
65	1175.0
66	731.5
67	288.0
68	340.5
69	393.0
70	271.0
71	149.0
72	617.5
73	1086.0
74	568.5
75	51.0
76	31.0
77	11.0
78	9.5
79	8.0
80	9.5
81	11.0
82	21.5
83	32.0
84	16.5
85	1.0
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	825053.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.13254292508543
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.1701921099724	33.32926198681268
2	6.463188564731398	5.058420078845455
3	2.152127905346452	2.526547129087327
4	1.1570717581656405	1.8111664097528397
5	0.7089388330048871	1.3871289656911863
6	0.5084953629160265	1.193922996991098
7	0.40159826787044445	1.1000893019256088
8	0.3359658228140683	1.051775758610654
9	0.24234899669740043	0.8535359264501169
>10	2.394294638039038	19.559826326438028
>50	0.25883488420547357	6.992955169763973
>100	0.17443219604248292	13.257316449953633
>500	0.022194777632645536	5.983248794763319
>1k	0.01031588256165215	5.894804704914127
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAAGTTCATAGGGATCATCACTAATGGAACGCCGGAGGTGAACACCACGCGG	2984	0.3616737348994549	No Hit
TTATTTCATAGGGACCAAAACCTGGCAGAGATACAATAAAAAAAGAAAACTT	2398	0.2906479947348837	No Hit
GCGATTCATAGGGTGGGCATGGTGGCTCACGCCTGTAATCCCAGCACTTTGG	2160	0.26180136306394863	No Hit
ATTTTTCATAGGGGGATCACTTGAGTCCAGGAGTTGGAGACTAGCCTGGACA	2044	0.24774166023273655	No Hit
CATGTTCATAGGGGGCCGCTGCGGGGTTGTAGGACCACGAGATCTGATTGAA	1998	0.2421662608341525	No Hit
TGGCTTCATAGGGGTACTTTACACTCAAAGACGACACCGCCCAGGTACGCTG	1971	0.23889374379585313	No Hit
GGTTTTCATAGGGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTC	1846	0.2237432019518746	No Hit
AGGGTTCATAGGGGAGGATAACTGATGCATCACCATCATCACCATGCTCCTG	1735	0.2102895207944217	No Hit
GCTCTTCATAGGGTAAACAATACATTTAGGCCAGGCGCAGTGGCTCACGCCT	1591	0.19283609659015843	No Hit
TGGATTCATAGGGAGGCCAACATAGTGAAACCGCATCTCTACAAAAATTAGC	1550	0.18786671886533352	No Hit
GGTTTTCATAGGGGGGGATGAGGCTGGGAAAGGAGAAGAGATGGACCCGCGA	1469	0.17804916775043542	No Hit
CGTATTCATAGGACGCAGAGTGCAGTTCATAGGGCGGCTGTGGTGGCTCACG	1417	0.17174654234334036	No Hit
GCTTTTCATAGGGCACCTGGCAGGAGGGCCAGTGCCATGGGCTTTAACACAT	1351	0.16374705624971972	No Hit
GTATTTCATAGGGTGGAGCAGGTCCCAAGGGTTGGGCTGTTCGCCCATTAAA	1346	0.16314103457596058	No Hit
TGATTTCATAGGGACGCAGAGTCATATTCATAGGGCGCCTGTAATCCCAACA	1339	0.16229260423269778	No Hit
GCGGTTCATAGGGTGATGTCACTGTGGTCAGGAGAATGGGATATAAGCTGAC	1319	0.1598685175376612	No Hit
ATAGTTCATAGGGGCTCAGATTGGACGCTGGCGGCAGGCTTAACACATGCAA	1313	0.15914129152915024	No Hit
AGACTTCATAGGGGTCGCCTGCTGGGTCTGTTCCAGAACCGTTACGGACCTA	1311	0.1588988828596466	No Hit
CCTCTTCATAGGGATCAACGCAGAGTGTTCTTCATAGGGAACTGCTCTGTCC	1224	0.14835410573623756	No Hit
TCGATTCATAGGGACGTCAGGCAAGTAAGTTACGCGTTGTTTTTGCGACAGA	1200	0.1454452017021937	No Hit
GGTTTTCATAGGGTCACCTCCTTACCTTAAAGAAGCGTACTTTGCAGTGCTC	1182	0.14326352367666076	No Hit
TGGGTTCATAGGGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAA	1175	0.14241509333339797	No Hit
ACCATTCATAGGGGTCTTCCAGAAAACAGAAGACAGAATGCCTCATACCTCG	1165	0.1412030499858797	No Hit
TTCCTTCATAGGGGTTGTCCGACCAATAAGTCTGCAAGAAGGCCTGGGTGGC	1158	0.14035461964261692	No Hit
AGTGTTCATAGGGGCAGAGTATTATTCATAGGGGTGGACTCTCTGACATAAA	1150	0.13938498496460228	No Hit
TTGATTCATAGGGACAGACAAAGGCTGGTACAGAAATCTAAGAAACACTGTC	1144	0.1386577589560913	No Hit
ATCCTTCATAGGGATCCGTTGAGCGATGGCCCTTCCATTCAGAACCACCGGA	1133	0.1373245112738212	No Hit
TGTTTTCATAGGGTCTGTGGACTTAGGATGCACAGACTAGCTCCATGGGACT	1122	0.1359912635915511	No Hit
TTCGTTCATAGGGGCCTTTTCCTTCAAAAACTATTCCATAAAATTAAAAAAA	1110	0.13453681157452915	No Hit
GGCCTTCATAGGGAGTCTCTGTGACCTTATCGACGCGCATGTGAGCCTATAG	1102	0.13356717689651454	No Hit
GCTGTTCATAGGGATCCACAGTGCGCACGCAAAAACGCTGGATACCCAAGGG	1100	0.13332476822701086	No Hit
GCAATTCATAGGGGTAACACTTTGGGAGGCCGAGGCGGGCGGATCACAAGGT	1046	0.12677973415041216	No Hit
GTAGTTCATAGGGGCTGATCAGCTTCGAGTCGGAAGTGAACAACATCCGCAA	1035	0.12544648646814205	No Hit
AGACTTCATAGGGGGTGCGGGCGGGCGCGACCCGCTCCGGGGACAGTGCCAG	990	0.1199922914043098	No Hit
TAGTTTCATAGGTGTTTAAAATATCACTTTGAGGACAAGAGTTGATCATGCT	989	0.11987108706955796	No Hit
TATCTTCATAGGGGGTTTCATTTGATGCTCGATGAGTTTTTCTAAGAATTAA	976	0.11829543071778419	No Hit
TAGGTTCATAGGGGCTGAGCCACTGTGGTGGGAGCCTGTGCCCAGGTTCAAG	965	0.11696218303551408	No Hit
TTAATTCATAGGGAACCATAAAACTCCTAAAAGAAAACATAGATGGTATGCT	958	0.11611375269225128	No Hit
TTGCTTCATAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	950	0.11514411801423666	No Hit
CGTTTTCATAGGGATTACTCCTCATATGCACGATTAGGGCAAGTCGGCCAAG	932	0.11296243998870376	No Hit
CTCATTCATAGGCTGCTTTAAGACCTGATAGTGCCTGCTATATCGAATCACA	922	0.11175039664118548	No Hit
TCTATTCATAGGGGCCAAATCAGTAAATTTGTGCTACCATGTATTGCGGGAT	919	0.11138678363693	No Hit
CTTGTTCATAGGGTCGAGACCAGCCTGGCCGACATGGAGAAACCCCGTCTCT	905	0.10968992295040439	No Hit
CTTTTTCATAGGGTAATTTTTTCTTCAGGCAAGGGAAACAAGCATAAATAAA	870	0.10544777123409042	No Hit
GGCGTTCATAGGGGAGAACATAGGCAAAACACTCTCCGACATATATCAATCA	867	0.10508415822983494	No Hit
TGTTTTCATAGGGGCCTGTTCCCTCTGGTATCTAATAAGCTAGCAGATTCCC	864	0.10472054522557946	No Hit
GTGGTTCATAGGCTCAGGAGACACTGGAGAAGTGTCTCACTCAAAGGCAGAA	850	0.10302368453905385	No Hit
GGTCTTCATAGGGACACCAAAATAACGATCATCACGACATAATTTTTCTTTA	848	0.1027812758695502	No Hit
TTCCTTCATAGGGACAAGATGAATCTCACAGCTATGATGTTGAGCAAAAGAC	848	0.1027812758695502	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.2579228243518901	0.0	0.0	0.0	0.0
11	0.27355818353487593	0.0	0.0	0.0	0.0
12	0.3767030724086816	0.0	0.0	0.0	0.0
13	0.48130241329950924	0.0	0.0	0.0	0.0
14	0.7681930736570862	0.0	0.0	0.0	0.0
15	1.2051347004374264	0.0	0.0	0.0	0.0
16	1.4213632336346878	0.0	0.0	0.0	0.0
17	1.569111317697166	0.0	0.0	0.0	0.0
18	1.7638866836433538	0.0	0.0	0.0	0.0
19	1.9504201548264173	0.0	0.0	0.0	0.0
20	2.1454379294421084	0.0	0.0	0.0	0.0
21	2.374150509118808	0.0	0.0	0.0	0.0
22	2.6346186245004866	0.0	0.0	0.0	0.0
23	2.830969646798448	0.0	0.0	0.0	0.0
24	3.0359261768637893	0.0	0.0	0.0	0.0
25	3.285849515122059	0.0	0.0	0.0	0.0
26	3.597102246764753	0.0	0.0	0.0	0.0
27	3.885447359139352	0.0	0.0	0.0	0.0
28	4.1611872206997615	0.0	0.0	0.0	0.0
29	4.560313095037531	0.0	0.0	0.0	0.0
30	5.067189622969676	0.0	0.0	0.0	0.0
31	5.442923060700343	0.0	0.0	0.0	0.0
32	5.671878049046546	0.0	0.0	0.0	0.0
33	5.861926445937413	0.0	0.0	0.0	0.0
34	6.059247102913389	0.0	0.0	0.0	0.0
35	6.248568273795744	0.0	0.0	0.0	0.0
36	6.446010135106472	0.0	0.0	0.0	0.0
37	6.649633417489543	0.0	0.0	0.0	0.0
38	6.850832613177578	0.0	0.0	0.0	0.0
39	7.065606694357817	0.0	0.0	0.0	0.0
40	7.249231261506837	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCACAC	105	0.0	46.000004	38
GTGTTTG	55	1.8189894E-12	46.000004	46
CTTAATG	55	1.8189894E-12	46.000004	24
CTTAACA	110	0.0	46.000004	39
TAATATA	55	1.8189894E-12	46.000004	36
TCGCATC	55	1.8189894E-12	46.000004	20
TCTGACG	110	0.0	46.000004	29
GCCTTGG	55	1.8189894E-12	46.000004	36
ACTGCGG	55	1.8189894E-12	46.000004	24
CATATCT	55	1.8189894E-12	46.000004	17
ATTAGCT	55	1.8189894E-12	46.000004	45
ATCTACT	55	1.8189894E-12	46.000004	33
TTGTGAG	55	1.8189894E-12	46.000004	29
GTCTGAC	110	0.0	46.000004	28
TTAGCTA	55	1.8189894E-12	46.000004	46
GCGCGTG	55	1.8189894E-12	46.000004	42
GCTATTA	55	1.8189894E-12	46.000004	42
TAGAAAC	55	1.8189894E-12	46.000004	28
TGGCGCT	55	1.8189894E-12	46.000004	46
TTGTCCG	110	0.0	46.000004	15
>>END_MODULE