SRR1528186_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1528186_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	781271
Sequences flagged as poor quality	0
Sequence length	59
%GC	37

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAAGTTCATAGGGATCATCACTAATGGAACGCCGGAGGTGAACACCACGCGGGCTGCTT	2637	0.3375269272761948	No Hit
TTATTTCATAGGGACCAAAACCTGGCAGAGATACAATAAAAAAAGAAAACTTCAGGCCA	2320	0.29695201792975806	No Hit
GCGATTCATAGGGTGGGCATGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCTG	1868	0.23909757305723622	No Hit
ATTTTTCATAGGGGGATCACTTGAGTCCAGGAGTTGGAGACTAGCCTGGACAACATGGT	1842	0.2357696625114717	No Hit
CATGTTCATAGGGGGCCGCTGCGGGGTTGTAGGACCACGAGATCTGATTGAACCGAGTT	1767	0.22616992055253557	No Hit
TGGCTTCATAGGGGTACTTTACACTCAAAGACGACACCGCCCAGGTACGCTGCGCGATG	1651	0.2113223196560476	No Hit
TGGATTCATAGGGAGGCCAACATAGTGAAACCGCATCTCTACAAAAATTAGCTAGGCGT	1399	0.17906718667402222	No Hit
GCTCTTCATAGGGTAAACAATACATTTAGGCCAGGCGCAGTGGCTCACGCCTGTAACCC	1341	0.17164338622577827	No Hit
GCTTTTCATAGGGCACCTGGCAGGAGGGCCAGTGCCATGGGCTTTAACACATAGCTTCT	1284	0.1643475823369868	No Hit
GGTTTTCATAGGGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCC	1232	0.15769176124545772	No Hit
AGACTTCATAGGGGTCGCCTGCTGGGTCTGTTCCAGAACCGTTACGGACCTAACCTTTT	1162	0.14873200208378398	No Hit
CGTATTCATAGGACGCAGAGTGCAGTTCATAGGGCGGCTGTGGTGGCTCACGCCTGTAA	1085	0.13887626700594288	No Hit
GCGGTTCATAGGGTGATGTCACTGTGGTCAGGAGAATGGGATATAAGCTGACCAGGCTG	1080	0.13823628420868045	No Hit
TGATTTCATAGGGACGCAGAGTCATATTCATAGGGCGCCTGTAATCCCAACACTTCCGG	1068	0.13670032549525069	No Hit
ACCATTCATAGGGGTCTTCCAGAAAACAGAAGACAGAATGCCTCATACCTCGCTTCTGA	1054	0.13490837366291594	No Hit
GTATTTCATAGGGTGGAGCAGGTCCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTAC	1045	0.1337564046278436	No Hit
ATAGTTCATAGGGGCTCAGATTGGACGCTGGCGGCAGGCTTAACACATGCAAGTCGAAC	1030	0.1318364562360564	No Hit
GGTTTTCATAGGGTCACCTCCTTACCTTAAAGAAGCGTACTTTGCAGTGCTCACACAGA	1029	0.13170845967660388	No Hit
AGTGTTCATAGGGGCAGAGTATTATTCATAGGGGTGGACTCTCTGACATAAACGACAGA	1012	0.1295325181659117	No Hit
CCTCTTCATAGGGATCAACGCAGAGTGTTCTTCATAGGGAACTGCTCTGTCCTCTGTAT	1004	0.12850854569029185	No Hit
GGTTTTCATAGGGGGGGATGAGGCTGGGAAAGGAGAAGAGATGGACCCGCGAAAAAGAC	985	0.1260766110606947	No Hit
AGGGTTCATAGGGGAGGATAACTGATGCATCACCATCATCACCATGCTCCTGGTATGAA	979	0.1253086317039798	No Hit
TCGATTCATAGGGACGTCAGGCAAGTAAGTTACGCGTTGTTTTTGCGACAGATGACGAG	964	0.12338868331219256	No Hit
TTCGTTCATAGGGGCCTTTTCCTTCAAAAACTATTCCATAAAATTAAAAAAAAATGGCT	944	0.12082875212314292	No Hit
TTGATTCATAGGGACAGACAAAGGCTGGTACAGAAATCTAAGAAACACTGTCATGGATC	943	0.12070075556369045	No Hit
ATCCTTCATAGGGATCCGTTGAGCGATGGCCCTTCCATTCAGAACCACCGGATCACTAT	930	0.11903680029080818	No Hit
TGGGTTCATAGGGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGG	924	0.1182688209340933	No Hit
GCTGTTCATAGGGATCCACAGTGCGCACGCAAAAACGCTGGATACCCAAGGGCTGCGCA	916	0.11724484845847342	No Hit
GGCGTTCATAGGGGAGAACATAGGCAAAACACTCTCCGACATATATCAATCACAGCAGG	909	0.11634887254230607	No Hit
GTAGTTCATAGGGGCTGATCAGCTTCGAGTCGGAAGTGAACAACATCCGCAAACAGTAT	898	0.11494091038832877	No Hit
TGTTTTCATAGGGTCTGTGGACTTAGGATGCACAGACTAGCTCCATGGGACTCTCTTCC	897	0.11481291382887628	No Hit
TTAATTCATAGGGAACCATAAAACTCCTAAAAGAAAACATAGATGGTATGCTCTTTGAC	880	0.11263697231818409	No Hit
GGCCTTCATAGGGAGTCTCTGTGACCTTATCGACGCGCATGTGAGCCTATAGACAGCGA	873	0.1117409964020167	No Hit
TTCCTTCATAGGGGTTGTCCGACCAATAAGTCTGCAAGAAGGCCTGGGTGGCAAACTGT	861	0.11020503768858693	No Hit
CTTGTTCATAGGGTCGAGACCAGCCTGGCCGACATGGAGAAACCCCGTCTCTACTAAAA	860	0.11007704112913445	No Hit
CTCATTCATAGGCTGCTTTAAGACCTGATAGTGCCTGCTATATCGAATCACAGGACCTT	817	0.10457318907267774	No Hit
CTTTTTCATAGGGTAATTTTTTCTTCAGGCAAGGGAAACAAGCATAAATAAACAAATGG	801	0.10252524412143801	No Hit
TAGTTTCATAGGTGTTTAAAATATCACTTTGAGGACAAGAGTTGATCATGCTTGCCCAC	801	0.10252524412143801	No Hit
TAGGTTCATAGGGGCTGAGCCACTGTGGTGGGAGCCTGTGCCCAGGTTCAAGAGGCAAA	788	0.10086128884855576	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAAGT	75	0.0	53.000004	30
TTTAGGC	150	0.0	53.000004	25
TTAGGCC	150	0.0	53.000004	26
ACTATGG	75	0.0	53.000004	35
TCCATCT	45	7.6397555E-11	53.000004	25
CTACTAA	75	0.0	53.000004	51
CCTAGGA	45	7.6397555E-11	53.000004	45
GCGGTAC	90	0.0	53.000004	53
ATGTGAA	45	7.6397555E-11	53.000004	48
ATCAGCT	90	0.0	53.000004	18
TAGCGAG	45	7.6397555E-11	53.000004	30
GTCTCTA	75	0.0	53.000004	47
TGGCGCT	45	7.6397555E-11	53.000004	46
TTGTCCG	90	0.0	53.000004	15
TAAGTCT	90	0.0	53.000004	27
CGACAGT	45	7.6397555E-11	53.000004	46
CTGACGA	75	0.0	53.000004	37
ATGTCCT	45	7.6397555E-11	53.000004	44
ACTTAAG	75	0.0	53.000004	29
TCCTAAC	75	0.0	53.000004	21

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content