##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528186_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	781271
Sequences flagged as poor quality	0
Sequence length	59
%GC	37
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.440319684206887	31.0	31.0	33.0	27.0	34.0
2	31.249117143731176	31.0	31.0	34.0	28.0	34.0
3	31.459780281106045	31.0	31.0	34.0	30.0	34.0
4	35.08418717704868	35.0	35.0	37.0	32.0	37.0
5	34.70349469006273	35.0	35.0	37.0	32.0	37.0
6	35.217109044108895	37.0	35.0	37.0	32.0	37.0
7	35.05811171795702	37.0	35.0	37.0	32.0	37.0
8	35.10103152427263	37.0	35.0	37.0	32.0	37.0
9	37.04331147578753	39.0	37.0	39.0	33.0	39.0
10	36.61678086093046	38.0	35.0	39.0	32.0	39.0
11	37.017381932773645	39.0	37.0	39.0	34.0	39.0
12	37.20117475242265	39.0	37.0	39.0	34.0	39.0
13	37.12138553715676	39.0	37.0	39.0	34.0	39.0
14	36.87770440730553	40.0	36.0	40.0	31.0	40.0
15	36.03620894670351	38.0	35.0	40.0	30.0	40.0
16	35.55789604375435	38.0	34.0	40.0	30.0	40.0
17	35.493296180198676	38.0	34.0	40.0	27.0	40.0
18	35.54767935837884	38.0	34.0	40.0	27.0	40.0
19	36.03412644268122	38.0	34.0	40.0	30.0	40.0
20	36.12566830203604	38.0	35.0	40.0	30.0	40.0
21	36.16250443188087	38.0	35.0	40.0	30.0	40.0
22	36.11490507135168	38.0	35.0	40.0	30.0	40.0
23	36.014710644577875	38.0	35.0	40.0	30.0	40.0
24	36.087378131275834	38.0	35.0	40.0	30.0	40.0
25	36.1809884150314	38.0	35.0	40.0	30.0	40.0
26	35.805532267292655	38.0	35.0	40.0	29.0	40.0
27	35.540773943996385	38.0	34.0	40.0	27.0	40.0
28	35.42534792664773	38.0	34.0	40.0	27.0	40.0
29	35.239480282769996	38.0	34.0	40.0	27.0	40.0
30	35.04300044414806	37.0	34.0	40.0	27.0	40.0
31	34.97811642823041	37.0	33.0	40.0	26.0	40.0
32	34.94701710418024	37.0	33.0	40.0	26.0	40.0
33	34.68658634455906	37.0	33.0	40.0	25.0	40.0
34	34.396073833535354	37.0	33.0	40.0	24.0	40.0
35	34.298240943283446	36.0	33.0	40.0	24.0	40.0
36	33.94218523406091	36.0	33.0	40.0	23.0	40.0
37	33.85120911949887	36.0	33.0	40.0	23.0	40.0
38	33.704824574315445	35.0	33.0	39.0	22.0	40.0
39	33.14407036738852	35.0	33.0	39.0	21.0	40.0
40	32.661996925522644	35.0	32.0	39.0	18.0	40.0
41	32.12087611085014	35.0	31.0	38.0	16.0	40.0
42	31.746228901367132	35.0	30.0	38.0	15.0	40.0
43	31.320365148584806	35.0	30.0	38.0	12.0	40.0
44	30.786774371504894	35.0	28.0	38.0	10.0	40.0
45	30.408186147956343	35.0	26.0	38.0	10.0	40.0
46	29.877152742134292	35.0	24.0	38.0	9.0	40.0
47	29.2879653794906	34.0	23.0	38.0	8.0	40.0
48	28.85636994077599	34.0	22.0	37.0	8.0	40.0
49	28.401705682151263	33.0	20.0	37.0	8.0	40.0
50	27.785184654236495	33.0	19.0	37.0	8.0	40.0
51	27.254541637920774	33.0	17.0	36.0	8.0	40.0
52	26.400517874079544	33.0	13.0	36.0	8.0	39.0
53	26.05214835825213	32.0	12.0	35.0	8.0	39.0
54	25.625968453967957	31.0	10.0	35.0	8.0	39.0
55	25.230123734274024	31.0	10.0	35.0	8.0	39.0
56	24.668517070261153	30.0	10.0	35.0	8.0	39.0
57	24.04875644942664	29.0	9.0	35.0	8.0	39.0
58	23.54168911939647	28.0	9.0	35.0	8.0	38.0
59	22.770746642330256	26.0	9.0	35.0	8.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	4.0
14	16.0
15	66.0
16	211.0
17	728.0
18	1547.0
19	2803.0
20	4568.0
21	6813.0
22	9426.0
23	13258.0
24	17546.0
25	22541.0
26	28780.0
27	34860.0
28	37665.0
29	37881.0
30	38680.0
31	43195.0
32	49749.0
33	57259.0
34	65534.0
35	69728.0
36	73261.0
37	78782.0
38	79317.0
39	7053.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.588638257403645	21.120328285575685	37.584269734829526	6.706763722191147
2	27.463197789243427	21.588155710374505	33.62354419913193	17.32510230125014
3	24.521196870228128	23.888765869973415	34.13706639565529	17.452970864143172
4	25.713484821528	22.109613693583917	33.16621249220821	19.010688992679878
5	0.0	0.0	100.0	0.0
6	0.0	0.0	100.0	0.0
7	0.0	0.0	0.0	100.0
8	0.0	100.0	0.0	0.0
9	0.0	0.0	100.0	0.0
10	0.0	100.0	0.0	0.0
11	99.44628688380857	0.42891647072526695	0.09471745399483661	0.03007919147133325
12	98.56131867175411	1.1480011417293103	0.1868749768006236	0.10380520971596283
13	95.44972231146427	2.6197055823139475	1.0466278666429447	0.8839442395788402
14	50.8858001896909	27.50095677428191	12.88157374329778	8.731669292729412
15	32.90497151436569	31.68247637503504	23.786624615530336	11.625927495068932
16	37.54587076699379	24.9554891964504	22.446244644943945	15.052395391611872
17	25.15068394961543	28.71730807875884	21.279300012415668	24.852707959210058
18	19.011712965155496	39.00426356539536	23.977979471911794	18.006043997537347
19	19.473268558541147	39.17321902387264	27.529756000158716	13.823756417427498
20	14.704244749901122	39.21148999514893	29.52867826912813	16.555586985821822
21	21.99326482104161	37.30229331435571	18.333971182854604	22.370470681748074
22	22.33616760381481	38.163326169792555	20.945612981923045	18.55489324446959
23	16.804412297397445	39.78069069503412	27.015977810516457	16.398919197051985
24	16.88825004383882	40.05703526689203	20.05731685932282	22.99739782994633
25	21.34355428526081	41.4505338096512	20.390107913899275	16.815803991188716
26	16.51693202486717	40.450496690648954	22.33872753500386	20.693843749480013
27	17.46461855105335	47.13332505622249	18.015387746377378	17.386668646346788
28	15.964626870829711	46.895379452200324	17.134899413903753	20.00509426306621
29	16.241099439247073	50.79300268408785	16.839867344365782	16.126030532299293
30	20.222944407254335	52.94731277623258	15.489759635261006	11.339983181252087
31	18.305171956977798	53.148779360810785	14.57266428678397	13.973384395427452
32	16.88108223650948	56.30939840337091	15.398114098693028	11.411405261426573
33	17.526824878947252	54.32468375250073	15.622748060532132	12.52574330801988
34	15.89397277001194	55.03084077100008	16.030161109269383	13.0450253497186
35	16.955448237551373	55.391407078977714	15.930323792896447	11.722820890574463
36	15.139561048599012	54.38945001158368	19.404022419877354	11.066966519939944
37	15.568989505562088	54.804798847007	17.730723398155057	11.895488249275859
38	14.356094108190373	56.31183033800051	17.56957572980438	11.762499824004731
39	15.926867885791228	52.70923917565096	20.691923801088226	10.671969137469585
40	16.012369587505486	51.906956741002794	19.72554977722199	12.355123894269722
41	15.149800773355212	53.834584926357174	19.53278695868655	11.482827341601059
42	15.469920168545869	50.6563023585926	18.4137130393935	15.460064433468029
43	15.761752324097527	51.96237925124573	17.06821320642901	15.207655218227734
44	17.60861468043739	52.63320921933619	17.295791089135523	12.462385011090902
45	17.290287237079067	49.6405216627777	19.540594748813152	13.528596351330076
46	20.374108343967716	49.25653198442026	17.289263264603445	13.080096407008579
47	20.932813325977797	48.97045967404396	16.034384995731315	14.062342004246926
48	20.968780359183945	47.07265468704202	17.634981971684603	14.323582982089441
49	21.46105512683819	47.31956005022585	16.657088257467638	14.56229656546832
50	20.251999626250047	48.085107472311144	16.276170496537055	15.386722404901757
51	19.785196173926845	47.99346193574316	17.626790191879643	14.594551698450346
52	20.90977394527635	48.16075343894756	15.805527147430276	15.123945468345813
53	19.648623844991047	48.15000172795355	16.077775829385708	16.123598597669694
54	18.006043997537347	48.23819135741631	17.672228970485275	16.083535674561066
55	20.307038146814612	48.30039768531022	16.268106713291548	15.12445745458362
56	22.287528911222868	44.63035233612921	16.967479914139908	16.11463883850802
57	21.08615320420187	42.75277080552075	18.106905286385903	18.05417070389148
58	22.539554136784805	42.40026828078861	18.760839708628634	16.299337873797953
59	22.5675853833049	40.111561801218784	20.31510193006012	17.005750885416198
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	1.0
2	2.0
3	4.0
4	55.0
5	104.0
6	502.0
7	900.0
8	1523.0
9	3900.0
10	5654.0
11	5573.5
12	5493.0
13	5850.0
14	6069.0
15	5931.0
16	6381.5
17	6832.0
18	7714.5
19	10741.5
20	12886.0
21	17781.0
22	22676.0
23	27503.5
24	32331.0
25	35225.5
26	39771.5
27	41423.0
28	41681.0
29	41939.0
30	41007.5
31	39271.5
32	38467.0
33	36548.5
34	34630.0
35	33643.0
36	32356.0
37	32056.0
38	31146.5
39	30237.0
40	32801.5
41	35366.0
42	37882.5
43	41672.5
44	42946.0
45	42195.5
46	41445.0
47	44916.0
48	42238.0
49	36089.0
50	32079.5
51	28070.0
52	24995.5
53	18388.0
54	14855.0
55	13065.0
56	11275.0
57	8997.0
58	6285.0
59	5851.0
60	4142.5
61	2434.0
62	2149.0
63	1864.0
64	1558.0
65	1292.5
66	1333.0
67	932.0
68	531.0
69	329.0
70	142.5
71	158.0
72	450.0
73	742.0
74	415.5
75	51.0
76	13.0
77	11.5
78	10.0
79	10.0
80	10.0
81	10.0
82	7.0
83	4.0
84	3.0
85	2.0
86	1.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	781271.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.65568346821345
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.85869123139126	52.04316934210058
2	3.8341035475295557	4.344475139463776
3	1.2203414639685366	2.0741783911721283
4	0.6118656183609252	1.3866265919575709
5	0.36554016480068047	1.0354963935932966
6	0.25467106961755703	0.8657138105258196
7	0.20015019368271797	0.7937752213572781
8	0.1513718607054008	0.6860860980895748
9	0.11914011644511778	0.6074968253112617
>10	1.179780580714456	14.021755949393091
>50	0.10966482485364641	4.269052161767985
>100	0.07674247683568947	8.894794875238317
>500	0.01339587613389027	5.387147881729644
>1k	0.004540974960640769	3.590231318299729
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAAGTTCATAGGGATCATCACTAATGGAACGCCGGAGGTGAACACCACGCGGGCTGCTT	2637	0.3375269272761948	No Hit
TTATTTCATAGGGACCAAAACCTGGCAGAGATACAATAAAAAAAGAAAACTTCAGGCCA	2320	0.29695201792975806	No Hit
GCGATTCATAGGGTGGGCATGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCTG	1868	0.23909757305723622	No Hit
ATTTTTCATAGGGGGATCACTTGAGTCCAGGAGTTGGAGACTAGCCTGGACAACATGGT	1842	0.2357696625114717	No Hit
CATGTTCATAGGGGGCCGCTGCGGGGTTGTAGGACCACGAGATCTGATTGAACCGAGTT	1767	0.22616992055253557	No Hit
TGGCTTCATAGGGGTACTTTACACTCAAAGACGACACCGCCCAGGTACGCTGCGCGATG	1651	0.2113223196560476	No Hit
TGGATTCATAGGGAGGCCAACATAGTGAAACCGCATCTCTACAAAAATTAGCTAGGCGT	1399	0.17906718667402222	No Hit
GCTCTTCATAGGGTAAACAATACATTTAGGCCAGGCGCAGTGGCTCACGCCTGTAACCC	1341	0.17164338622577827	No Hit
GCTTTTCATAGGGCACCTGGCAGGAGGGCCAGTGCCATGGGCTTTAACACATAGCTTCT	1284	0.1643475823369868	No Hit
GGTTTTCATAGGGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCC	1232	0.15769176124545772	No Hit
AGACTTCATAGGGGTCGCCTGCTGGGTCTGTTCCAGAACCGTTACGGACCTAACCTTTT	1162	0.14873200208378398	No Hit
CGTATTCATAGGACGCAGAGTGCAGTTCATAGGGCGGCTGTGGTGGCTCACGCCTGTAA	1085	0.13887626700594288	No Hit
GCGGTTCATAGGGTGATGTCACTGTGGTCAGGAGAATGGGATATAAGCTGACCAGGCTG	1080	0.13823628420868045	No Hit
TGATTTCATAGGGACGCAGAGTCATATTCATAGGGCGCCTGTAATCCCAACACTTCCGG	1068	0.13670032549525069	No Hit
ACCATTCATAGGGGTCTTCCAGAAAACAGAAGACAGAATGCCTCATACCTCGCTTCTGA	1054	0.13490837366291594	No Hit
GTATTTCATAGGGTGGAGCAGGTCCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTAC	1045	0.1337564046278436	No Hit
ATAGTTCATAGGGGCTCAGATTGGACGCTGGCGGCAGGCTTAACACATGCAAGTCGAAC	1030	0.1318364562360564	No Hit
GGTTTTCATAGGGTCACCTCCTTACCTTAAAGAAGCGTACTTTGCAGTGCTCACACAGA	1029	0.13170845967660388	No Hit
AGTGTTCATAGGGGCAGAGTATTATTCATAGGGGTGGACTCTCTGACATAAACGACAGA	1012	0.1295325181659117	No Hit
CCTCTTCATAGGGATCAACGCAGAGTGTTCTTCATAGGGAACTGCTCTGTCCTCTGTAT	1004	0.12850854569029185	No Hit
GGTTTTCATAGGGGGGGATGAGGCTGGGAAAGGAGAAGAGATGGACCCGCGAAAAAGAC	985	0.1260766110606947	No Hit
AGGGTTCATAGGGGAGGATAACTGATGCATCACCATCATCACCATGCTCCTGGTATGAA	979	0.1253086317039798	No Hit
TCGATTCATAGGGACGTCAGGCAAGTAAGTTACGCGTTGTTTTTGCGACAGATGACGAG	964	0.12338868331219256	No Hit
TTCGTTCATAGGGGCCTTTTCCTTCAAAAACTATTCCATAAAATTAAAAAAAAATGGCT	944	0.12082875212314292	No Hit
TTGATTCATAGGGACAGACAAAGGCTGGTACAGAAATCTAAGAAACACTGTCATGGATC	943	0.12070075556369045	No Hit
ATCCTTCATAGGGATCCGTTGAGCGATGGCCCTTCCATTCAGAACCACCGGATCACTAT	930	0.11903680029080818	No Hit
TGGGTTCATAGGGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGG	924	0.1182688209340933	No Hit
GCTGTTCATAGGGATCCACAGTGCGCACGCAAAAACGCTGGATACCCAAGGGCTGCGCA	916	0.11724484845847342	No Hit
GGCGTTCATAGGGGAGAACATAGGCAAAACACTCTCCGACATATATCAATCACAGCAGG	909	0.11634887254230607	No Hit
GTAGTTCATAGGGGCTGATCAGCTTCGAGTCGGAAGTGAACAACATCCGCAAACAGTAT	898	0.11494091038832877	No Hit
TGTTTTCATAGGGTCTGTGGACTTAGGATGCACAGACTAGCTCCATGGGACTCTCTTCC	897	0.11481291382887628	No Hit
TTAATTCATAGGGAACCATAAAACTCCTAAAAGAAAACATAGATGGTATGCTCTTTGAC	880	0.11263697231818409	No Hit
GGCCTTCATAGGGAGTCTCTGTGACCTTATCGACGCGCATGTGAGCCTATAGACAGCGA	873	0.1117409964020167	No Hit
TTCCTTCATAGGGGTTGTCCGACCAATAAGTCTGCAAGAAGGCCTGGGTGGCAAACTGT	861	0.11020503768858693	No Hit
CTTGTTCATAGGGTCGAGACCAGCCTGGCCGACATGGAGAAACCCCGTCTCTACTAAAA	860	0.11007704112913445	No Hit
CTCATTCATAGGCTGCTTTAAGACCTGATAGTGCCTGCTATATCGAATCACAGGACCTT	817	0.10457318907267774	No Hit
CTTTTTCATAGGGTAATTTTTTCTTCAGGCAAGGGAAACAAGCATAAATAAACAAATGG	801	0.10252524412143801	No Hit
TAGTTTCATAGGTGTTTAAAATATCACTTTGAGGACAAGAGTTGATCATGCTTGCCCAC	801	0.10252524412143801	No Hit
TAGGTTCATAGGGGCTGAGCCACTGTGGTGGGAGCCTGTGCCCAGGTTCAAGAGGCAAA	788	0.10086128884855576	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.23231375540625468	0.0	0.0	0.0	0.0
11	0.2448574182325979	0.0	0.0	0.0	0.0
12	0.33394302361152534	0.0	0.0	0.0	0.0
13	0.42302862899045274	0.0	0.0	0.0	0.0
14	0.6662220919501683	0.0	0.0	0.0	0.0
15	1.0345961900544114	0.0	0.0	0.0	0.0
16	1.2081595246719767	0.0	0.0	0.0	0.0
17	1.3374360497189834	0.0	0.0	0.0	0.0
18	1.5084394531474994	0.0	0.0	0.0	0.0
19	1.6727870354844863	0.0	0.0	0.0	0.0
20	1.8357266556674956	0.0	0.0	0.0	0.0
21	2.027081512048956	0.0	0.0	0.0	0.0
22	2.233795955564714	0.0	0.0	0.0	0.0
23	2.4017274415663707	0.0	0.0	0.0	0.0
24	2.5773387211351757	0.0	0.0	0.0	0.0
25	2.794420885966585	0.0	0.0	0.0	0.0
26	3.0630856642573447	0.0	0.0	0.0	0.0
27	3.302183237314581	0.0	0.0	0.0	0.0
28	3.5325770443290483	0.0	0.0	0.0	0.0
29	3.8727918993537456	0.0	0.0	0.0	0.0
30	4.302860339114084	0.0	0.0	0.0	0.0
31	4.62438769645872	0.0	0.0	0.0	0.0
32	4.821502398015541	0.0	0.0	0.0	0.0
33	4.9861059734714335	0.0	0.0	0.0	0.0
34	5.169397046607387	0.0	0.0	0.0	0.0
35	5.333872625503826	0.0	0.0	0.0	0.0
36	5.502444094302745	0.0	0.0	0.0	0.0
37	5.678311366990456	0.0	0.0	0.0	0.0
38	5.853026670643093	0.0	0.0	0.0	0.0
39	6.028765946771351	0.0	0.0	0.0	0.0
40	6.192985532548885	0.0	0.0	0.0	0.0
41	6.356821128648062	0.0	0.0	0.0	0.0
42	6.5172008176420215	0.0	0.0	0.0	0.0
43	6.687436241713823	0.0	0.0	0.0	0.0
44	6.855111734596574	0.0	0.0	0.0	0.0
45	7.022531234360421	0.0	0.0	0.0	0.0
46	7.168319315576797	0.0	0.0	0.0	0.0
47	7.309627517212338	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAAGT	75	0.0	53.000004	30
TTTAGGC	150	0.0	53.000004	25
TTAGGCC	150	0.0	53.000004	26
ACTATGG	75	0.0	53.000004	35
TCCATCT	45	7.6397555E-11	53.000004	25
CTACTAA	75	0.0	53.000004	51
CCTAGGA	45	7.6397555E-11	53.000004	45
GCGGTAC	90	0.0	53.000004	53
ATGTGAA	45	7.6397555E-11	53.000004	48
ATCAGCT	90	0.0	53.000004	18
TAGCGAG	45	7.6397555E-11	53.000004	30
GTCTCTA	75	0.0	53.000004	47
TGGCGCT	45	7.6397555E-11	53.000004	46
TTGTCCG	90	0.0	53.000004	15
TAAGTCT	90	0.0	53.000004	27
CGACAGT	45	7.6397555E-11	53.000004	46
CTGACGA	75	0.0	53.000004	37
ATGTCCT	45	7.6397555E-11	53.000004	44
ACTTAAG	75	0.0	53.000004	29
TCCTAAC	75	0.0	53.000004	21
>>END_MODULE