FastQCFastQC Report
Fri 17 Jun 2016
SRR1528185_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528185_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences921121
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC34220.3715038523711868No Hit
TACATAGAGAGGGCAGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGG25960.2818305086953831No Hit
TGTTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC20850.2263546265908605No Hit
GTTTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC19390.21050437456099688No Hit
TTGGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC18810.20420769909707845No Hit
TCACTAGAGAGGGACGGGTGGATCACGAGGTCAGGAGATTGAGACCATCCTG18680.20279637528620018No Hit
TGCTTAGAGAGGGGCGCAGTGGCTCACACCTGTAATCCCAGCACTTTGGGAG18580.20171074158552457No Hit
TTTGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC18390.199648037554241No Hit
TTTCTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC18000.19541406612160617No Hit
TGGTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC17920.1945455591610657No Hit
TTGCTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC17830.19356848883045766No Hit
CGGTTAGAGAGGGGATCAAGGCTTTCGTTGTTCTCGCTGCCTGCTGCCTCGC17830.19356848883045766No Hit
GGTTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC17670.19183147490937671No Hit
TGGGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC17540.19042015109849847No Hit
GTATTAGAGAGGGAATGCTTGTACCAACAGGGAGGGAATACGATGGCATTAA17200.18672899651620145No Hit
GTGTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC17190.1866204331461339No Hit
TTCTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC16890.1833635320441071No Hit
TTGTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC16880.18325496867403956No Hit
TGGCTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC16500.17912956061147234No Hit
TTATTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC15720.17066161774620273No Hit
TGACTAGAGAGGGCGGGTGGATCACTTGAGGTCACGAGTTTGAGACCAGCCT15600.16935885730539202No Hit
GGGTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC15090.1638221254319465No Hit
CTATTAGAGAGGGCGGGTTTCACTGTGTTAGCCACGATGGTCTCAATCTCCT14900.16175942140066288No Hit
GAAATAGAGAGGGAAAGAAAGAAAGAAAGAAAGAAAAAGAGAGAGGAAAGAG14480.15719975985782542No Hit
GCTCTAGAGAGGGAGAAGCAACCCCCAGGCATCCTTCCCAGTGTTACACTGT14370.15600556278708227No Hit
GCACTAGAGAGGGGTCCCGTCCTCTTCTGTCGATGGTGGATGACATGCTGTT13620.14786331003201536No Hit
TTTATAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC13480.14634342285106952No Hit
TGTGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC13270.14406359207965078No Hit
GGATTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC13230.14362933859938054No Hit
TACGTAGAGAGGGGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCA13050.14167519793816447No Hit
TGTCTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC12960.14069812760755643No Hit
GTGCTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC12820.13917824042661062No Hit
GGTGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC12780.13874398694634038No Hit
TCTGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC12750.1384182968361377No Hit
GCTTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC12710.13798404335586748No Hit
GTTGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC12700.1378754799857999No Hit
TCTTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC12650.1373326631354621No Hit
ACATTAGAGAGGGGAGGGGGTTTTGTCACTTTGAGAGGCTGAGGTGGGAGGA12610.13689840965519187No Hit
GGCTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC12330.13385863529330025No Hit
AGGTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC12290.13342438181302999No Hit
TGCCTAGAGAGGGGGGTGGATCAGTTGAGGTCAGGAGTTTGAGACCAGCCTG12030.13060173419127347No Hit
TTAGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC11930.12951610049059786No Hit
TATTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC11920.1294075371205303No Hit
GTGGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC11380.12354511513688211No Hit
TATCTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC11350.12321942502667946No Hit
TTGATAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC11160.12115672099539583No Hit
TTCATAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC11060.12007108729472024No Hit
TGTATAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC10910.11844263674370685No Hit
TTCGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC10910.11844263674370685No Hit
ATTTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC10860.11789981989336906No Hit
CGTGTAGAGAGGGGTAGGGACTAGGTTTGTCCCTGTCGCCACTGTGTTCTCA10800.11724843967296371No Hit
ATCTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC10690.11605424260222054No Hit
TACGTAGAGAGGGGTCGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10500.11399153857093694No Hit
GCGTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC10460.1135572850906667No Hit
GTTATAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC10450.11344872172059915No Hit
GATTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC10420.11312303161039647No Hit
GGGCTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC10390.11279734150019377No Hit
GGCCTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC10370.11258021476005867No Hit
TGCTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC10140.1100832572485048No Hit
TAGTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC10120.10986613050836969No Hit
GTTCTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC9960.10812911658728876No Hit
TGATTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC9930.10780342647708607No Hit
TCATTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC9920.10769486310701851No Hit
TGACTAGAGAGGGGATCAAGACCATCCTGGCTAAGAAGGTGAAACCCTGTCT9870.10715204625668072No Hit
GCGGTAGAGAGGGGACTTGTGTTTCCCTGATTCATCGCCTTCAACCTCTCGC9870.10715204625668072No Hit
GGTCTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC9810.10650066603627537No Hit
AGTTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC9770.10606641255600513No Hit
GCCTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC9760.10595784918593758No Hit
TGATTAGAGAGGGGTAAGGAACTAATGGTAATTCTGCTCACTTTCTCCAGAA9730.1056321590757349No Hit
ATGTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC9700.10530646896553222No Hit
TTACTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC9510.1032437649342486No Hit
TGAGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC9370.10172387775330277No Hit
TGGATAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC9310.10107249753289742No Hit
TCACTAGAGAGGGTCCCCCAAATAAACAAACCAAAAAACTCCAGGAAATAAG9280.10074680742269473No Hit
ACCTTAGAGAGGGGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTT9270.10063824405262718No Hit
GTATTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC9270.10063824405262718No Hit
ACTGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC9260.10052968068255963No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAAA1100.046.00000432
AGCACCA1100.046.00000434
TCGCCCT551.8189894E-1246.00000436
TTGATAT351.0192707E-746.00000419
TGTTTCC551.8189894E-1246.00000420
TTCTAGG700.046.00000419
TAATACT700.046.00000415
GCCTTTG351.0192707E-746.00000416
ACTGCCG551.8189894E-1246.00000420
CATATGA551.8189894E-1246.00000420
GTACATG700.046.00000445
TTAATGA700.046.00000436
CTACTAA700.046.00000441
TTCAGCC1100.046.00000422
GGATGTT700.046.00000442
TGCCAGC351.0192707E-746.00000433
TAAAGGA700.046.00000438
TTAGCTT850.046.00000438
TCATTTG700.046.00000424
TAAAGAA700.046.00000417
Produced by FastQC (version 0.11.5)