FastQCFastQC Report
Fri 17 Jun 2016
SRR1528184_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528184_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences914028
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC33100.36213332633135964No Hit
TACATAGAGAGGGCAGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGG26990.29528635884239873No Hit
TGTTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC21580.23609780006739398No Hit
TTGGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC19660.2150918790233997No Hit
TGCTTAGAGAGGGGCGCAGTGGCTCACACCTGTAATCCCAGCACTTTGGGAG18990.20776168782575588No Hit
GTTTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC18870.20644881776050622No Hit
TCACTAGAGAGGGACGGGTGGATCACGAGGTCAGGAGATTGAGACCATCCTG18430.20163496085459087No Hit
TTTCTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC18230.19944684407917482No Hit
CGGTTAGAGAGGGGATCAAGGCTTTCGTTGTTCTCGCTGCCTGCTGCCTCGC18010.19703991562621714No Hit
TGGTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC17880.1956176397221967No Hit
TTTGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC17860.19539882804465508No Hit
GTGTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC17620.19277308791415582No Hit
TTGCTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC17550.19200724704276018No Hit
GGTTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC17020.18620873758790757No Hit
GTATTAGAGAGGGAATGCTTGTACCAACAGGGAGGGAATACGATGGCATTAA16930.18522408503897037No Hit
TTGTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC16910.18500527336142877No Hit
TGGCTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC16750.1832547799410959No Hit
TGGGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC16720.1829265624247835No Hit
TTCTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC16690.18259834490847107No Hit
TTATTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC16330.17865973471272215No Hit
TGACTAGAGAGGGCGGGTGGATCACTTGAGGTCACGAGTTTGAGACCAGCCT14840.16235826473587242No Hit
CTATTAGAGAGGGCGGGTTTCACTGTGTTAGCCACGATGGTCTCAATCTCCT14550.1591854954115191No Hit
GGGTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC14450.1580914370238111No Hit
GAAATAGAGAGGGAAAGAAAGAAAGAAAGAAAGAAAAAGAGAGAGGAAAGAG14280.15623153776470744No Hit
GCTCTAGAGAGGGAGAAGCAACCCCCAGGCATCCTTCCCAGTGTTACACTGT13940.1525117392465001No Hit
GCACTAGAGAGGGGTCCCGTCCTCTTCTGTCGATGGTGGATGACATGCTGTT13830.15130827502002128No Hit
GGATTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC13680.1496671874384592No Hit
GGTGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC13390.1464944181141059No Hit
GTGCTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC13050.1427746195958986No Hit
TTTATAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC12810.1401488794653993No Hit
TACGTAGAGAGGGGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCA12720.13916422691646207No Hit
GTTGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC12720.13916422691646207No Hit
GCTTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC12710.13905482107769127No Hit
TCTTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC12560.13741373349612923No Hit
TGTGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC12520.136976110141046No Hit
TGCCTAGAGAGGGGGGTGGATCAGTTGAGGTCAGGAGTTTGAGACCAGCCTG12490.1366478926247336No Hit
ACATTAGAGAGGGGAGGGGGTTTTGTCACTTTGAGAGGCTGAGGTGGGAGGA12420.13588205175333798No Hit
AGGTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC12240.13391274665546352No Hit
GGCTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC12180.13325631162283869No Hit
TATTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC12120.13259987659021388No Hit
TGTCTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC12040.13172462988004743No Hit
TTAGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC12040.13172462988004743No Hit
TGTATAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC11810.12920829558831895No Hit
TCTGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC11550.12636374378027806No Hit
TATCTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC11260.12319097445592476No Hit
GTGGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC11230.12286275693961235No Hit
TTCGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC10970.12001820513157146No Hit
CGTGTAGAGAGGGGTAGGGACTAGGTTTGTCCCTGTCGCCACTGTGTTCTCA10740.11750187083984298No Hit
GTTATAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC10740.11750187083984298No Hit
GATTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC10660.11662662412967656No Hit
ATTTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC10650.11651721829090574No Hit
GGGCTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC10570.11564197158073933No Hit
ATCTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC10570.11564197158073933No Hit
TACGTAGAGAGGGGTCGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10550.11542315990319771No Hit
TTGATAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC10440.11421969567671887No Hit
TTCATAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC10360.11334444896655245No Hit
GCGTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC10130.11082811467482397No Hit
GTTCTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC10100.11049989715851155No Hit
AGTTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC10090.11039049131974077No Hit
TGCTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC10040.10984346212588673No Hit
TGGATAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC10040.10984346212588673No Hit
GGCCTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC10030.10973405628711592No Hit
GCGGTAGAGAGGGGACTTGTGTTTCCCTGATTCATCGCCTTCAACCTCTCGC9930.1086399978994079No Hit
TGACTAGAGAGGGGATCAAGACCATCCTGGCTAAGAAGGTGAAACCCTGTCT9900.1083117803830955No Hit
TAGTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC9840.10765534535047067No Hit
TGATTAGAGAGGGGTAAGGAACTAATGGTAATTCTGCTCACTTTCTCCAGAA9810.10732712783415826No Hit
TCATTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC9780.10699891031784584No Hit
TGATTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC9720.10634247528522102No Hit
TTACTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC9520.10415435850980495No Hit
GCCTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC9440.10327911179963851No Hit
GTATTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC9430.10316970596086772No Hit
ACCTTAGAGAGGGGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTT9400.1028414884445553No Hit
GGTCTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC9350.10229445925070128No Hit
TGAGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC9290.10163802421807647No Hit
AGGCTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC9220.10087218334668085No Hit
ATGTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC9200.10065337166913924No Hit
GTCTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCC9180.10043455999159763No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGTTA750.046.00000429
CTGCTCA1100.046.00000434
AACCGTG700.046.00000444
CAGCGCT1100.046.00000420
CTCACTT1100.046.00000437
CTCACTG551.8189894E-1246.00000440
AGCAACC1450.046.00000417
TCTGAGT351.0192707E-746.00000442
TTGGCGT1550.046.00000422
TCCATCT351.0192707E-746.00000431
CGAACAG1100.046.00000416
AATCATG551.8189894E-1246.00000432
ACGCCCG551.8189894E-1246.00000436
ACGCCAC700.046.00000432
CCCTCCC700.046.00000423
GTACATG551.8189894E-1246.00000445
GGTAAGT551.8189894E-1246.00000422
CTCAAAC750.046.00000445
TCATTTG551.8189894E-1246.00000424
GTTTCAC1400.046.00000417
Produced by FastQC (version 0.11.5)