SRR1528183_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1528183_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	860699
Sequences flagged as poor quality	0
Sequence length	59
%GC	44

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TTTTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC	2147	0.24944841344070345	No Hit
TACATAGAGAGGGCAGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCA	2035	0.23643573421137937	No Hit
GAAATAGAGAGGGAAAGAAAGAAAGAAAGAAAGAAAAAGAGAGAGGAAAGAGAAAGAAA	1621	0.18833529491727072	No Hit
TCACTAGAGAGGGACGGGTGGATCACGAGGTCAGGAGATTGAGACCATCCTGGCTAACA	1600	0.18589541756177247	No Hit
TGTTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC	1570	0.18240987848248924	No Hit
TTGGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC	1548	0.17985381649101487	No Hit
GTTTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC	1485	0.17253418442452007	No Hit
GTATTAGAGAGGGAATGCTTGTACCAACAGGGAGGGAATACGATGGCATTAACAGATAT	1478	0.17172089197268733	No Hit
TTGCTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC	1454	0.16893246070926074	No Hit
TTTCTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC	1446	0.16800298362145186	No Hit
CGGTTAGAGAGGGGATCAAGGCTTTCGTTGTTCTCGCTGCCTGCTGCCTCGCTCGTTGC	1441	0.16742206044157135	No Hit
TTTGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC	1439	0.16718969116961913	No Hit
GGTTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC	1427	0.16579547553790583	No Hit
TGCTTAGAGAGGGGCGCAGTGGCTCACACCTGTAATCCCAGCACTTTGGGAGACTGAGG	1424	0.16544692162997748	No Hit
GTGTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC	1402	0.16289085963850314	No Hit
TGGTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC	1307	0.1518533192207729	No Hit
CTATTAGAGAGGGCGGGTTTCACTGTGTTAGCCACGATGGTCTCAATCTCCTGATCTTA	1295	0.1504591035890596	No Hit
TGGCTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC	1279	0.14860014941344188	No Hit
TTATTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC	1265	0.14697356450977636	No Hit
TTCTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC	1259	0.1462764566939197	No Hit
GGGTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC	1250	0.14523079497013475	No Hit
TGGGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC	1215	0.14116433271097098	No Hit
GCTCTAGAGAGGGAGAAGCAACCCCCAGGCATCCTTCCCAGTGTTACACTGTAGCCCAG	1209	0.14046722489511432	No Hit
TGACTAGAGAGGGCGGGTGGATCACTTGAGGTCACGAGTTTGAGACCAGCCTGGCCAAC	1163	0.13512273164021338	No Hit
TTGTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC	1163	0.13512273164021338	No Hit
GTGCTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC	1121	0.13024297692921682	No Hit
TACGTAGAGAGGGGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCG	1116	0.12966205374933631	No Hit
GGATTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC	1076	0.12501466831029198	No Hit
GCTTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC	1037	0.12048346750722377	No Hit
AGGTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC	1035	0.12025109823527157	No Hit
GCACTAGAGAGGGGTCCCGTCCTCTTCTGTCGATGGTGGATGACATGCTGTTGAAGGCT	1004	0.11664937452001223	No Hit
GGCTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC	998	0.11595226670415558	No Hit
GTTGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC	977	0.11351238934865732	No Hit
TTTATAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC	971	0.11281528153280065	No Hit
TATTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC	947	0.11002685026937407	No Hit
TGTCTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC	945	0.10979448099742185	No Hit
TGTGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC	938	0.10898118854558911	No Hit
TTCGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC	929	0.10793552682180414	No Hit
GCGTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC	916	0.10642512655411475	No Hit
TCACTAGAGAGGGTCCCCCAAATAAACAAACCAAAAAACTCCAGGAAATAAGTTTATAT	909	0.10561183410228198	No Hit
TTAGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC	904	0.10503091092240145	No Hit
GGTGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC	901	0.10468235701447312	No Hit
TGCCTAGAGAGGGGGGTGGATCAGTTGAGGTCAGGAGTTTGAGACCAGCCTGGGCAACA	899	0.1044499877425209	No Hit
GTGGTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC	890	0.10340432601873593	No Hit
GATTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC	886	0.1029395874748315	No Hit
GTTATAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC	882	0.10247484893092706	No Hit
TGCTTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC	878	0.10201011038702265	No Hit
TATCTAGAGAGGGGGGGACAAGTTTGTACAAAAAAGCAGGCTCTCAGTATCCCTGCATC	861	0.1000349715754288	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAC	40	1.6007107E-9	53.000004	20
TCCGGAC	50	3.6379788E-12	53.000004	21
CCTTCAA	55	0.0	53.000004	38
CAGGTTA	20	3.1378E-4	53.000004	29
CTTAAAT	20	3.1378E-4	53.000004	31
ACAACAT	45	7.6397555E-11	53.000004	20
ATCCTAA	55	0.0	53.000004	23
CTATGTT	45	7.6397555E-11	53.000004	20
GGAATTG	25	1.4784186E-5	53.000004	35
CATGGGT	55	0.0	53.000004	50
AACCGTG	90	0.0	53.000004	44
AGCGTGT	20	3.1378E-4	53.000004	30
TGTCACC	45	7.6397555E-11	53.000004	45
AGCGTGA	20	3.1378E-4	53.000004	15
CATGGAT	45	7.6397555E-11	53.000004	30
GGAATGT	20	3.1378E-4	53.000004	13
GGAATAT	20	3.1378E-4	53.000004	17
CAGAAAT	75	0.0	53.000004	34
AATCCTG	20	3.1378E-4	53.000004	20
TAATACT	45	7.6397555E-11	53.000004	15

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content