FastQCFastQC Report
Fri 17 Jun 2016
SRR1528182_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528182_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences562993
Sequences flagged as poor quality0
Sequence length52
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGGTTGCCGGGTGGGTAGATCACCTGAGGTGAGGCGTTGGAGACCAGCCT41030.7287834839864794No Hit
GTACGTTGCCGGGAATGGACAAAAAAAATAATCACTTCAATTGATGCTGAAA33300.5914815992383564No Hit
GTTCGTTGCCGGGGATTTCGCGGTTTGTTTGCTTTTTCTTTTTCCTGAACCT30840.5477865621775049No Hit
TGATGTTGCCGGGTGCTGCTGCTGCTAAGTCGCTTCAGTCGTGTCCGACTCT22940.4074651016975344No Hit
GGGCGTTGCCGGGTACACTACAAAAAATCAGCCGGGTGTGGTGGCGAGTGCC17900.31794356235335075No Hit
GGTTGTTGCCGGGCGGGGGAAGTATTTGAAACCTATACTCAGGAAGAAGACT17350.3081743467503148No Hit
AGTAGTTGCCGGGGTCTAGTGGAGCAAAGTGACGGAGAAGCAAGGCCCTACC16220.28810304923862284No Hit
CGTAGTTGCCGGAGAGACAGGGTTTCACCATCTTGGCCAGGCTGGCCTCGAC15020.26678839701381724No Hit
TTCAGTTGCCGGGACTCTAGCCCAAGCCGGCCTCAAGCTCATAGCGAGCCTC15010.26661077491194385No Hit
GACAGTTGCCGGGATGTTCTGTGCGGGCTATGAGGCCAAGTTAGAGGATGCC14600.25932826873513526No Hit
TTCTGTTGCCGGGATGCAATACAGAGTTCAAACATCTCTCACTGAGGTATGG14200.25222338466020006No Hit
GGTCGTTGCCGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTG13840.24582898899275837No Hit
CTACGTTGCCGGGGGGGGCCGAGGCGGGTGGATCACCTGAGGTCAGGAGTTC13720.24369752377027776No Hit
TTATGTTGCCGGGAGTGCTATTGGCTGGGCACAGTGGCTCATGCCTATAATC13640.24227654695529072No Hit
CGCGGTTGCCGGGTTAGGCAAAATATCTTGGAAATTTTAAGTAAGAATCTTT13430.23854648281594976No Hit
AGAGGTTGCCGGGTGGGTAGATCGCCTGAGGTGAGGCGTTGGAGACCAGCCT13360.2373031281028361No Hit
ATGTGTTGCCGGGGATGCTCTTGTCCAGCCTTATGGCCACACAGCCCATTTG13350.23712550600096272No Hit
TTGTGTTGCCGGGACCTGCCAGAAAATATATGCCACCAGGGGGATAGCACTA12580.22344860415671242No Hit
CTCTGTTGCCGGGACGCAGAGTCTTTGGGAATGGATTGTGCCTACCAAGCTG12510.2222052494435988No Hit
TCTAGTTGCCGGGGGTTGGTTGCATTACATGACACACAAAACTGTCCTCTAC11830.21012694651620892No Hit
AAGGGTTGCCGGGATCGCGGCATTTCACTCTCGTTGCCCAGGCTGGAGTGCA11710.20799548129372833No Hit
CGGGGTTGCCGGGGAAGAGGGAAATGAAGGTTTGAGGACTCAAAGAGGGGCT11670.2072849928862348No Hit
TTTAGTTGCCGGGCAATTAATTACAATTGATAAATATATATATCTTATTGAA11500.20426541715438734No Hit
AGCAGTTGCCGGGATATAACCTTATTTTCTAGTACTCTCTGGGATATGCTAA11490.20408779505251398No Hit
CGCGGTTGCCGGGGGGTGGCTCACAATCATCCCTAACGGAATCTGATGCTTC10820.1921871142269975No Hit
TAATGTTGCCGGGTTCTTCTTGCCTCAGAGGAAATTTAAGCGGCAGATTAGA10510.1866808290689227No Hit
TGTAGTTGCCGGGGCTTTCTGCCTCCTCTGCCGCCATGGCGCCTGTGAAAAA10510.1866808290689227No Hit
TAGCGTTGCCGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTG10330.18348363123520187No Hit
CAAAGTTGCCGGGGGGGAATTCGATAAAATTGGTTTTGCCTTTCAGCAATTC10230.18170741021646805No Hit
GGCGGTTGCCGGGGCCAACATGGTAAAAAACAGTCTCTACTAAAAATACAAA10220.1815297881145947No Hit
AACGGTTGCCGGGATCAACCCAGAGTGCACGTTGCGGGGCCAAATCAGTAAA10220.1815297881145947No Hit
GTTTGTTGCCGGGTCAGGGAAAGGTATAGTCTCATTATTTCCTTCTTAGACA10030.17815496817900045No Hit
TACTGTTGCCGGGCTCTCCTTTGAGAGATTTTTGGTTGTTGGCAATTTTCAC9920.17620112505839328No Hit
TTCTGTTGCCGGGATAGGCTATGCCACTTATTTCCACTAACGGGAAAGAGGA9640.1712277062059386No Hit
GGTTGTTGCCGGGATGCATTAGACATTGCTAAACTAGTTTGATTTTAGTTAT9530.16927386308533143No Hit
GTTAGTTGCCGGGGTCAAGAGATCGAGACCATGCTGGCCAATGTGGTGAAAC9200.16341233372350988No Hit
TGAAGTTGCCGGGATTGCCCATTATTGTAGTGTATGTTCCAAGAATGAAATG8950.15897178117667538No Hit
TTTTGTTGCCGGGGAGGTCATATCCATGACCGCGATACTCTCACGTCAAGCA8770.15577458334295455No Hit
TCTAGTTGCCGGGACAGCAAATAACTGAAAACATACCTAAGCTCCAACAATG8720.1548864728335876No Hit
AACTGTTGCCGGGAAGGGCCAAGGACCCTGAGCTTGGTGGGCCTCGGAGCTG8670.1539983623242207No Hit
CTTTGTTGCCGGGTGCTGTATGCCTGAAATATTGTAAATCAACAATACTTCA8660.15382074022234735No Hit
AGATGTTGCCGGGAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGC8470.15044592028675313No Hit
TGGCGTTGCCGGGTCGCAGACGGTATCAACAGGAACATCCTCGTGTTAGATA8380.1488473213698927No Hit
AATTGTTGCCGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCC8340.14813683296239918No Hit
TAATGTTGCCGGGCTAGATCAAGTTATACTCTAGGTGAAGGTTTATTACAGT8290.14724872245303228No Hit
GCCAGTTGCCGGGGGGGCTGGCGAGATGGCTCAGAGGTTAAATCACTGACTG8200.14565012353617185No Hit
GGGTGTTGCCGGGACTAGATGTGATAGATAGAGTGCCTGATGAACTATGGAC7820.1389004836649834No Hit
GTCTGTTGCCGGGGCTCAGCTGGCGAGTATCAGGCAGATGAGCAGGCAGGTG7620.1353480416275158No Hit
TTGTGTTGCCGGGGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCT7580.13463755322002227No Hit
AATGGTTGCCGGGATAGATCAGGGAAAGGAAGAGATCAACTTTACTAAACAT7580.13463755322002227No Hit
CGTAGTTGCCGGGCAGGCTGAAATGGTAGAAGTAGAATTTAGACTATGAATA7550.13410468691440214No Hit
AGTAGTTGCCGGGATGGATAACAAACAAGGCCCTACTATATAGCACTGGGAA7170.12735504704321368No Hit
GTAAGTTGCCGGGCCATGTTGCCCAGGCTGCTCTCGAACTCCTGACCTTGGG7100.12611169233010003No Hit
CGATGTTGCCGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG7000.12433547131136621No Hit
GATTGTTGCCGGGAGCAACAAATCTAAAATGAGAAAATAAGGCTAGAAATGA6890.12238162819075904No Hit
GCCAGTTGCCGGGATGTCTTCCTCGTAAGGCACAGTGCCTTGCTTAGCAATT6830.12131589557951876No Hit
ATAGGTTGCCGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAG6780.12042778507015185No Hit
TCTAGTTGCCGGGGGGGCTGGAGAGATAGCTCAGTGGTTAAGAGCACTGACT6470.11492149991207706No Hit
TATCGTTGCCGGGAGTGGTATCAACGCAGAGTGGGCGTTGCCGGGTACACTA6410.11385576730083677No Hit
TATTGTTGCCGGGATTCTTGATATAAAAAAAGAAATCACTGAATTTACTCAA6240.1108361915689893No Hit
GTTCGTTGCCGGGGAGTGACGAGAGAAATTGCCCCACGACGGTCTGTGATGC6080.10799423793901523No Hit
GATAGTTGCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG5960.10586277271653467No Hit
GAAAGTTGCCGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG5860.10408655169780086No Hit
CACTGTTGCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5800.10302081908656058No Hit
GGTGGTTGCCGGGCCAGAATTTGTATTTGGAATTTTCCCAACTGCAACTATA5720.10159984227157354No Hit
GTATGTTGCCGGGGGGCAAGAGGTCCCGGGGAAACGAAACTGGAAACCGACC5710.10142222016970015No Hit
AAATGTTGCCGGTATATAAGCCTGTGAAAAATTCTGGCTGACCGTCGAGACT5650.10035648755845987No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTCAT1050.046.00000415
TCGCCTG1050.046.00000422
TCTGCCG1500.046.00000427
CTTGTCT351.01832484E-746.00000443
TTGATCC351.01832484E-746.00000421
ATCCTGC351.01832484E-746.00000424
CTAGATG750.046.00000415
GTCATAT1050.046.00000417
TTTAGAG351.01832484E-746.00000432
AACAATG750.046.00000446
TTAGGCA1650.046.00000414
AAGTAAG1650.046.00000439
CCTTATT750.046.00000420
GATACTC1050.046.00000434
ACTATAC351.01832484E-746.00000435
GATACCC750.046.00000445
GCCCATA351.01832484E-746.00000443
CTCACGT1050.046.00000440
TCTTGGA1650.046.00000426
CTCACCC351.01832484E-746.00000417
Produced by FastQC (version 0.11.5)