FastQCFastQC Report
Fri 17 Jun 2016
SRR1528181_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528181_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences561793
Sequences flagged as poor quality0
Sequence length52
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGGTTGCCGGGTGGGTAGATCACCTGAGGTGAGGCGTTGGAGACCAGCCT40080.7134300356181014No Hit
GTACGTTGCCGGGAATGGACAAAAAAAATAATCACTTCAATTGATGCTGAAA32380.5763688760806917No Hit
GTTCGTTGCCGGGGATTTCGCGGTTTGTTTGCTTTTTCTTTTTCCTGAACCT31170.55483069386767No Hit
TGATGTTGCCGGGTGCTGCTGCTGCTAAGTCGCTTCAGTCGTGTCCGACTCT23480.4179475358361532No Hit
GGGCGTTGCCGGGTACACTACAAAAAATCAGCCGGGTGTGGTGGCGAGTGCC18770.3341088265606727No Hit
GGTTGTTGCCGGGCGGGGGAAGTATTTGAAACCTATACTCAGGAAGAAGACT16550.29459249225248446No Hit
AGTAGTTGCCGGGGTCTAGTGGAGCAAAGTGACGGAGAAGCAAGGCCCTACC15960.2840904034048128No Hit
CGTAGTTGCCGGAGAGACAGGGTTTCACCATCTTGGCCAGGCTGGCCTCGAC15940.28373440039302733No Hit
TTCAGTTGCCGGGACTCTAGCCCAAGCCGGCCTCAAGCTCATAGCGAGCCTC15310.2725203055217847No Hit
GACAGTTGCCGGGATGTTCTGTGCGGGCTATGAGGCCAAGTTAGAGGATGCC14960.2662902528155388No Hit
TTCTGTTGCCGGGATGCAATACAGAGTTCAAACATCTCTCACTGAGGTATGG14680.2613062106505421No Hit
AGAGGTTGCCGGGTGGGTAGATCGCCTGAGGTGAGGCGTTGGAGACCAGCCT14030.249736112767514No Hit
TTATGTTGCCGGGAGTGCTATTGGCTGGGCACAGTGGCTCATGCCTATAATC13980.2488461052380503No Hit
CTACGTTGCCGGGGGGGGCCGAGGCGGGTGGATCACCTGAGGTCAGGAGTTC13890.24724409168501563No Hit
GGTCGTTGCCGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTG13590.24190404650823344No Hit
ATGTGTTGCCGGGGATGCTCTTGTCCAGCCTTATGGCCACACAGCCCATTTG13030.23193596217824003No Hit
CGCGGTTGCCGGGTTAGGCAAAATATCTTGGAAATTTTAAGTAAGAATCTTT12930.23015594711931261No Hit
CTCTGTTGCCGGGACGCAGAGTCTTTGGGAATGGATTGTGCCTACCAAGCTG12550.2233918898953885No Hit
AAGGGTTGCCGGGATCGCGGCATTTCACTCTCGTTGCCCAGGCTGGAGTGCA12530.22303588688360304No Hit
TTTAGTTGCCGGGCAATTAATTACAATTGATAAATATATATATCTTATTGAA12250.2180518447186063No Hit
TCTAGTTGCCGGGGGTTGGTTGCATTACATGACACACAAAACTGTCCTCTAC12240.21787384321271358No Hit
TTGTGTTGCCGGGACCTGCCAGAAAATATATGCCACCAGGGGGATAGCACTA12190.2169838356832499No Hit
CGCGGTTGCCGGGGGGTGGCTCACAATCATCCCTAACGGAATCTGATGCTTC11090.1974036700350485No Hit
AGCAGTTGCCGGGATATAACCTTATTTTCTAGTACTCTCTGGGATATGCTAA10990.1956236549761211No Hit
CGGGGTTGCCGGGGAAGAGGGAAATGAAGGTTTGAGGACTCAAAGAGGGGCT10880.19366563841130097No Hit
TGTAGTTGCCGGGGCTTTCTGCCTCCTCTGCCGCCATGGCGCCTGTGAAAAA10780.19188562335237355No Hit
GGCGGTTGCCGGGGCCAACATGGTAAAAAACAGTCTCTACTAAAAATACAAA10550.18779158871684054No Hit
TAATGTTGCCGGGTTCTTCTTGCCTCAGAGGAAATTTAAGCGGCAGATTAGA10350.18423155859898574No Hit
AACGGTTGCCGGGATCAACCCAGAGTGCACGTTGCGGGGCCAAATCAGTAAA10310.18351955257541477No Hit
GTTTGTTGCCGGGTCAGGGAAAGGTATAGTCTCATTATTTCCTTCTTAGACA10040.17871351191631082No Hit
CAAAGTTGCCGGGGGGGAATTCGATAAAATTGGTTTTGCCTTTCAGCAATTC9970.17746750137506165No Hit
TACTGTTGCCGGGCTCTCCTTTGAGAGATTTTTGGTTGTTGGCAATTTTCAC9440.16803342156274642No Hit
GTTAGTTGCCGGGGTCAAGAGATCGAGACCATGCTGGCCAATGTGGTGAAAC9410.16749941704506818No Hit
GGTTGTTGCCGGGATGCATTAGACATTGCTAAACTAGTTTGATTTTAGTTAT9200.16376138542132068No Hit
TAGCGTTGCCGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTG9140.16269337638596423No Hit
TCTAGTTGCCGGGACAGCAAATAACTGAAAACATACCTAAGCTCCAACAATG9050.16109136283292957No Hit
TTCTGTTGCCGGGATAGGCTATGCCACTTATTTCCACTAACGGGAAAGAGGA8990.16002335379757313No Hit
TGAAGTTGCCGGGATTGCCCATTATTGTAGTGTATGTTCCAAGAATGAAATG8840.15735333120918202No Hit
AACTGTTGCCGGGAAGGGCCAAGGACCCTGAGCTTGGTGGGCCTCGGAGCTG8800.15664132518561105No Hit
CTTTGTTGCCGGGTGCTGTATGCCTGAAATATTGTAAATCAACAATACTTCA8720.15521731313846915No Hit
TAATGTTGCCGGGCTAGATCAAGTTATACTCTAGGTGAAGGTTTATTACAGT8660.15414930410311273No Hit
TTTTGTTGCCGGGGAGGTCATATCCATGACCGCGATACTCTCACGTCAAGCA8540.15201328603239983No Hit
TGGCGTTGCCGGGTCGCAGACGGTATCAACAGGAACATCCTCGTGTTAGATA8420.14987726796168696No Hit
AATGGTTGCCGGGATAGATCAGGGAAAGGAAGAGATCAACTTTACTAAACAT7840.13955318061990804No Hit
AGATGTTGCCGGGAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGC7760.13812916857276614No Hit
GCCAGTTGCCGGGGGGGCTGGCGAGATGGCTCAGAGGTTAAATCACTGACTG7750.13795116706687338No Hit
AATTGTTGCCGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCC7420.13207711737241298No Hit
GGGTGTTGCCGGGACTAGATGTGATAGATAGAGTGCCTGATGAACTATGGAC7390.13154311285473477No Hit
GTCTGTTGCCGGGGCTCAGCTGGCGAGTATCAGGCAGATGAGCAGGCAGGTG7390.13154311285473477No Hit
AGTAGTTGCCGGGATGGATAACAAACAAGGCCCTACTATATAGCACTGGGAA7310.13011910080759284No Hit
CGTAGTTGCCGGGCAGGCTGAAATGGTAGAAGTAGAATTTAGACTATGAATA7280.1295850962899146No Hit
GTAAGTTGCCGGGCCATGTTGCCCAGGCTGCTCTCGAACTCCTGACCTTGGG7190.12798308273687997No Hit
ATAGGTTGCCGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAG7080.1260250661720598No Hit
CGATGTTGCCGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG6860.12210903304241953No Hit
TCTAGTTGCCGGGGGGGCTGGAGAGATAGCTCAGTGGTTAAGAGCACTGACT6820.12139702701884857No Hit
TTGTGTTGCCGGGGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCT6810.12121902551295585No Hit
TATCGTTGCCGGGAGTGGTATCAACGCAGAGTGGGCGTTGCCGGGTACACTA6780.12068502099527763No Hit
GATTGTTGCCGGGAGCAACAAATCTAAAATGAGAAAATAAGGCTAGAAATGA6520.11605698184206639No Hit
TATTGTTGCCGGGATTCTTGATATAAAAAAAGAAATCACTGAATTTACTCAA6300.11214094871242611No Hit
GTTCGTTGCCGGGGAGTGACGAGAGAAATTGCCCCACGACGGTCTGTGATGC6120.10893692160635679No Hit
GGTGGTTGCCGGGCCAGAATTTGTATTTGGAATTTTCCCAACTGCAACTATA6030.10733490805332213No Hit
GATAGTTGCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG5970.1062668990179657No Hit
GTATGTTGCCGGGGGGCAAGAGGTCCCGGGGAAACGAAACTGGAAACCGACC5960.10608889751207294No Hit
GCCAGTTGCCGGGATGTCTTCCTCGTAAGGCACAGTGCCTTGCTTAGCAATT5830.10377487793546733No Hit
GAAAGTTGCCGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG5650.10057085082939801No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTCAG1700.046.00000440
ATTTATC206.308685E-446.00000435
ATTTATA206.308685E-446.00000425
TATTTAG206.308685E-446.00000419
ATTTAAG850.046.00000434
CTAGATG1100.046.00000415
CTAGATA551.8189894E-1246.00000421
ATCCTAC206.308685E-446.00000443
TGTTTAC405.6024874E-946.00000428
TTCTACA206.308685E-446.00000426
ACTATAC405.6024874E-946.00000435
AACCGAC405.6024874E-946.00000445
CGGCGCA405.6024874E-946.00000426
TCTGAGC206.308685E-446.00000414
TCTGAGA206.308685E-446.00000420
GCCTTGC551.8189894E-1246.00000437
CTGTCAG405.6024874E-946.00000428
ACAGTCT850.046.00000430
ACAGTCC551.8189894E-1246.00000438
TGCCCCC206.308685E-446.00000416
Produced by FastQC (version 0.11.5)