FastQCFastQC Report
Fri 17 Jun 2016
SRR1528180_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528180_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences526232
Sequences flagged as poor quality0
Sequence length59
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGGTTGCCGGGTGGGTAGATCACCTGAGGTGAGGCGTTGGAGACCAGCCTGGCCAAC34600.6575046747442193No Hit
GTACGTTGCCGGGAATGGACAAAAAAAATAATCACTTCAATTGATGCTGAAAAAAATTG29660.5636297298529925No Hit
GTTCGTTGCCGGGGATTTCGCGGTTTGTTTGCTTTTTCTTTTTCCTGAACCTGACATGT22680.43098861338725125No Hit
TGATGTTGCCGGGTGCTGCTGCTGCTAAGTCGCTTCAGTCGTGTCCGACTCTGTGCGAC18210.3460450903783882No Hit
GGGCGTTGCCGGGTACACTACAAAAAATCAGCCGGGTGTGGTGGCGAGTGCCTGTGGTC16750.31830067346721597No Hit
GGTTGTTGCCGGGCGGGGGAAGTATTTGAAACCTATACTCAGGAAGAAGACTAATTTTG14350.27269341279131637No Hit
GACAGTTGCCGGGATGTTCTGTGCGGGCTATGAGGCCAAGTTAGAGGATGCCTGCCAGG11950.2270861521154168No Hit
AGTAGTTGCCGGGGTCTAGTGGAGCAAAGTGACGGAGAAGCAAGGCCCTACCCTGTGGA11880.22575594034570304No Hit
GGTCGTTGCCGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGTTCTTCC11680.22195533528937805No Hit
ATGTGTTGCCGGGGATGCTCTTGTCCAGCCTTATGGCCACACAGCCCATTTGCAGCTTG11610.22062512351966432No Hit
TTCAGTTGCCGGGACTCTAGCCCAAGCCGGCCTCAAGCTCATAGCGAGCCTCCCTTACC11600.2204350932668481No Hit
CGCGGTTGCCGGGTTAGGCAAAATATCTTGGAAATTTTAAGTAAGAATCTTTACATTGA11190.21264385290138188No Hit
CTCTGTTGCCGGGACGCAGAGTCTTTGGGAATGGATTGTGCCTACCAAGCTGTTCCAAG10980.20865321759224068No Hit
TTCTGTTGCCGGGATGCAATACAGAGTTCAAACATCTCTCACTGAGGTATGGGGCTAAC10660.20257224950212074No Hit
CGTAGTTGCCGGAGAGACAGGGTTTCACCATCTTGGCCAGGCTGGCCTCGACCTCCTGA10640.20219218899648825No Hit
AGAGGTTGCCGGGTGGGTAGATCGCCTGAGGTGAGGCGTTGGAGACCAGCCTGGCCAAC10620.20181212849085575No Hit
TCTAGTTGCCGGGGGTTGGTTGCATTACATGACACACAAAACTGTCCTCTACCTCACGT10490.1993417352042445No Hit
AGCAGTTGCCGGGATATAACCTTATTTTCTAGTACTCTCTGGGATATGCTAAAGTATTT10150.19288070660849208No Hit
TTTAGTTGCCGGGCAATTAATTACAATTGATAAATATATATATCTTATTGAAAAGGGTG10110.19212058559722708No Hit
TTGTGTTGCCGGGACCTGCCAGAAAATATATGCCACCAGGGGGATAGCACTACCGGTTA9850.1871797990240046No Hit
TTATGTTGCCGGGAGTGCTATTGGCTGGGCACAGTGGCTCATGCCTATAATCCCAGCAC9690.18413931497894465No Hit
AACGGTTGCCGGGATCAACCCAGAGTGCACGTTGCGGGGCCAAATCAGTAAATTTGTGC9430.17919852840572217No Hit
CGCGGTTGCCGGGGGGTGGCTCACAATCATCCCTAACGGAATCTGATGCTTCTGATGTG9170.17425774183249973No Hit
GTTAGTTGCCGGGGTCAAGAGATCGAGACCATGCTGGCCAATGTGGTGAAACCCCGTCT9160.17406771157968348No Hit
GGCGGTTGCCGGGGCCAACATGGTAAAAAACAGTCTCTACTAAAAATACAAATATTAGC8630.16399610818042232No Hit
TCTAGTTGCCGGGACAGCAAATAACTGAAAACATACCTAAGCTCCAACAATGAGGGAAA8540.1622858359050761No Hit
CTTTGTTGCCGGGTGCTGTATGCCTGAAATATTGTAAATCAACAATACTTCAATTTTAA8460.1607655938825461No Hit
CAAAGTTGCCGGGGGGGAATTCGATAAAATTGGTTTTGCCTTTCAGCAATTCAACTTAA8460.1607655938825461No Hit
CGGGGTTGCCGGGGAAGAGGGAAATGAAGGTTTGAGGACTCAAAGAGGGGCTTCAAATG8360.15886529135438363No Hit
TAATGTTGCCGGGTTCTTCTTGCCTCAGAGGAAATTTAAGCGGCAGATTAGACAATTTA8240.15658492832058862No Hit
GGTTGTTGCCGGGATGCATTAGACATTGCTAAACTAGTTTGATTTTAGTTATTGATGTG8170.1552547165508749No Hit
GTTTGTTGCCGGGTCAGGGAAAGGTATAGTCTCATTATTTCCTTCTTAGACAACCTATG8150.1548746560452424No Hit
TGTAGTTGCCGGGGCTTTCTGCCTCCTCTGCCGCCATGGCGCCTGTGAAAAAGCTTGTG8070.1533544140227124No Hit
TACTGTTGCCGGGCTCTCCTTTGAGAGATTTTTGGTTGTTGGCAATTTTCACCAGAGAT7820.14860365770230619No Hit
TAGCGTTGCCGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGTTCTTCC7750.14727344593259245No Hit
TGAAGTTGCCGGGATTGCCCATTATTGTAGTGTATGTTCCAAGAATGAAATGAGCTTTT7460.14176256860092126No Hit
TTCTGTTGCCGGGATAGGCTATGCCACTTATTTCCACTAACGGGAAAGAGGAGGAATTA7360.13986226607275878No Hit
AACTGTTGCCGGGAAGGGCCAAGGACCCTGAGCTTGGTGGGCCTCGGAGCTGATCCTCC7010.13321120722419008No Hit
TAATGTTGCCGGGCTAGATCAAGTTATACTCTAGGTGAAGGTTTATTACAGTGGAGGAT6970.1324510862129251No Hit
GGGTGTTGCCGGGACTAGATGTGATAGATAGAGTGCCTGATGAACTATGGACTGAGGTT6730.12789036014533514No Hit
AGATGTTGCCGGGAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTC6620.1258000273643564No Hit
TTGTGTTGCCGGGGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTC6580.12503990635309142No Hit
TTTTGTTGCCGGGGAGGTCATATCCATGACCGCGATACTCTCACGTCAAGCAACACACA6570.12484987610027518No Hit
AATGGTTGCCGGGATAGATCAGGGAAAGGAAGAGATCAACTTTACTAAACATCTGATCC6540.12427978534182642No Hit
AGTAGTTGCCGGGATGGATAACAAACAAGGCCCTACTATATAGCACTGGGAACTCTGCT6450.12256951306648019No Hit
TGGCGTTGCCGGGTCGCAGACGGTATCAACAGGAACATCCTCGTGTTAGATATTGAGGC6350.12066921053831771No Hit
CGTAGTTGCCGGGCAGGCTGAAATGGTAGAAGTAGAATTTAGACTATGAATAGGAACGA6340.12047918028550145No Hit
GCCAGTTGCCGGGGGGGCTGGCGAGATGGCTCAGAGGTTAAATCACTGACTGCTCTTCC6230.11838884750452272No Hit
TCTAGTTGCCGGGGGGGCTGGAGAGATAGCTCAGTGGTTAAGAGCACTGACTGCTCTTC6050.11496830295383025No Hit
AATTGTTGCCGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGG6020.11439821219538152No Hit
GATTGTTGCCGGGAGCAACAAATCTAAAATGAGAAAATAAGGCTAGAAATGATTTTTCT5960.11325803067848401No Hit
ATAGGTTGCCGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGG5940.11287797017285152No Hit
TATTGTTGCCGGGATTCTTGATATAAAAAAAGAAATCACTGAATTTACTCAAGGCTTAA5530.10508672980738534No Hit
TATCGTTGCCGGGAGTGGTATCAACGCAGAGTGGGCGTTGCCGGGTACACTACAAAAAA5530.10508672980738534No Hit
GTAAGTTGCCGGGCCATGTTGCCCAGGCTGCTCTCGAACTCCTGACCTTGGGTGATCTG5490.10432660879612034No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCAC150.006694257353.00000453
ATTTAGA750.053.00000437
TATTTCG150.006694257353.00000430
TTGATCC307.0031456E-753.00000421
CTAGATG600.053.00000415
CTAGATA600.053.00000421
GCCCCAC750.053.00000431
GGAATTT600.053.00000429
TTTAGAC750.053.00000438
TGTCAAG307.0031456E-753.00000426
AGACTAT750.053.00000441
TCTGAGC150.006694257353.00000414
TTGGCGA307.0031456E-753.00000414
CTCACGG307.0031456E-753.00000437
AAATCTA600.053.00000420
CTGTCAA150.006694257353.00000443
GGTACCT307.0031456E-753.00000412
CTCACAG600.053.00000452
CTACTTT150.006694257353.00000418
CATATGT600.053.00000417
Produced by FastQC (version 0.11.5)