##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528179_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14912353
Sequences flagged as poor quality	0
Sequence length	52
%GC	38
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.849276100156697	31.0	31.0	34.0	27.0	34.0
2	31.559099761117512	31.0	31.0	34.0	28.0	34.0
3	31.74945677586897	31.0	31.0	34.0	30.0	34.0
4	35.47609810470554	37.0	35.0	37.0	33.0	37.0
5	35.189573905606984	37.0	35.0	37.0	33.0	37.0
6	35.35953413924684	37.0	35.0	37.0	33.0	37.0
7	35.362981080182315	37.0	35.0	37.0	32.0	37.0
8	35.324532486590144	37.0	35.0	37.0	32.0	37.0
9	37.4400075561516	39.0	37.0	39.0	34.0	39.0
10	37.766348409268474	39.0	37.0	39.0	35.0	39.0
11	37.820011905565806	39.0	37.0	39.0	35.0	39.0
12	37.68094176686939	39.0	37.0	39.0	35.0	39.0
13	37.59281047062124	39.0	37.0	39.0	35.0	39.0
14	38.024885609936945	40.0	38.0	40.0	33.0	40.0
15	36.989760167292175	39.0	36.0	40.0	31.0	40.0
16	35.882657753608704	38.0	35.0	40.0	31.0	40.0
17	35.345451653404396	38.0	34.0	40.0	27.0	40.0
18	35.40699801030729	38.0	34.0	40.0	27.0	40.0
19	35.6860785819649	38.0	34.0	40.0	27.0	40.0
20	35.870014745493215	38.0	34.0	40.0	30.0	40.0
21	36.00144926826773	38.0	35.0	40.0	30.0	40.0
22	36.12225615903808	38.0	35.0	40.0	30.0	40.0
23	36.139561342197304	38.0	35.0	40.0	30.0	40.0
24	36.123072395080776	38.0	35.0	40.0	30.0	40.0
25	36.26629600305197	39.0	35.0	40.0	30.0	40.0
26	36.0734104805593	39.0	35.0	40.0	30.0	40.0
27	35.82573823192088	38.0	35.0	40.0	29.0	40.0
28	35.50057184134523	38.0	35.0	40.0	27.0	40.0
29	35.29051987972656	37.0	34.0	40.0	28.0	40.0
30	35.05412217642648	36.0	34.0	40.0	27.0	40.0
31	34.803229309284724	35.0	33.0	40.0	26.0	40.0
32	34.54216219264659	35.0	33.0	40.0	25.0	40.0
33	34.14321435389841	35.0	33.0	40.0	23.0	40.0
34	33.78085061425249	35.0	33.0	40.0	22.0	40.0
35	33.55114025264826	35.0	33.0	40.0	21.0	40.0
36	33.30856934515968	35.0	33.0	39.0	21.0	40.0
37	33.11952003818579	35.0	33.0	40.0	21.0	40.0
38	32.76821545198132	35.0	33.0	39.0	18.0	40.0
39	32.268931133805644	35.0	32.0	39.0	16.0	40.0
40	31.76168871538918	35.0	31.0	39.0	15.0	40.0
41	31.075248822234826	35.0	30.0	38.0	10.0	40.0
42	30.31080641666677	35.0	25.0	38.0	9.0	40.0
43	29.446479304775043	35.0	23.0	37.0	8.0	40.0
44	28.6026849015712	34.0	21.0	36.0	8.0	40.0
45	27.951050850258174	34.0	20.0	35.0	8.0	40.0
46	27.329328242162724	33.0	17.0	35.0	8.0	40.0
47	26.676880570088436	33.0	15.0	35.0	8.0	40.0
48	26.199745003353932	33.0	10.0	35.0	8.0	39.0
49	25.89228809162444	33.0	10.0	35.0	8.0	39.0
50	25.530991252688292	33.0	10.0	35.0	8.0	39.0
51	25.132685901413414	33.0	10.0	35.0	8.0	38.0
52	24.36338309588031	31.0	9.0	35.0	8.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	11.0
12	16.0
13	52.0
14	191.0
15	692.0
16	2592.0
17	8242.0
18	19453.0
19	38074.0
20	65285.0
21	97868.0
22	138386.0
23	185384.0
24	244695.0
25	322711.0
26	418001.0
27	529286.0
28	659713.0
29	806737.0
30	934967.0
31	952815.0
32	843152.0
33	784884.0
34	911100.0
35	1182666.0
36	1648983.0
37	2045904.0
38	1835980.0
39	234511.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.95526118513959	23.941285456426627	36.240444415445374	2.863008942988407
2	29.35098840538445	21.621624702687765	30.655873020173274	18.371513871754512
3	17.696898671859497	22.40467349451827	35.02863029060538	24.86979754301685
4	14.219607059999184	25.725846216220877	33.123558703311275	26.930988020468664
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	100.0	0.0	0.0	0.0
8	0.0	0.0	0.0	100.0
9	0.0	0.0	100.0	0.0
10	0.0	0.0	100.0	0.0
11	99.54677843261892	0.2818267512846564	0.13718827605542866	0.034206540040998226
12	99.22971244041769	0.5726862823056831	0.10438325863128374	0.09321801864534725
13	96.717828500975	2.1700867730263624	0.5253094531761687	0.5867752728224714
14	60.23477984996733	26.930505199280084	5.243941046728173	7.5907739040244016
15	43.5091564691367	34.23668283603533	13.361928865283701	8.89223182954427
16	44.59974224054379	32.54624538461503	14.616191019619773	8.237821355221406
17	30.235496705315384	35.47003615056591	18.901537537369187	15.39292960674952
18	23.639877623605074	45.715602360003146	17.435363822194926	13.209156194196852
19	25.293399371648455	46.663065178245176	17.25368223244179	10.789853217664575
20	18.45115925032086	50.81892844140693	18.92535671600585	11.804555592266357
21	19.110753346571126	52.38300085841584	15.309596010770399	13.196649784242634
22	21.82273984528129	52.40692062480012	16.055031690840472	9.715307839078111
23	16.784460507339116	49.700634098455154	19.21475437176145	14.300151022444279
24	17.87874790785867	52.15172950908552	16.155921201704384	13.81360138135142
25	17.803890506079085	46.57470890073485	21.640682727937033	13.980717865249032
26	19.120570710738942	46.541756354614186	21.25799999503767	13.079672939609196
27	24.05783983252006	48.0775502028419	17.457607126118862	10.40700283851918
28	26.460673241841846	46.617425164224585	16.713599792065008	10.208301801868558
29	30.1005347714073	44.751716915499514	15.958896627514116	9.188851685579063
30	33.56105505281427	46.037047272150815	12.202507545254596	8.199390129780323
31	30.601334343413143	48.72563035491448	11.121014906232437	9.552020395439943
32	28.278830309341522	53.18408168046987	11.820549044138104	6.716538966050495
33	26.796964905538385	55.78717188360549	11.078191349145236	6.337671861710891
34	23.745863580348452	57.82295389600824	11.826939719036963	6.604242804606356
35	23.615468330182367	58.487651143987804	11.054814756598104	6.842065769231724
36	19.149888686245557	60.735371540628094	11.883637679446027	8.23110209368032
37	17.82445064169283	62.33379802637451	12.727669469734254	7.114081862198407
38	17.112799033123746	63.131539335207535	12.297945200197447	7.457716431471277
39	17.605618643818318	63.73851933360215	10.922883866818335	7.7329781557612
40	18.177694693788432	63.87672689883347	10.563450315319118	7.38212809205898
41	18.82130874986664	63.41077092260357	10.43710539845724	7.330814929072561
42	20.326322747322305	60.87746849876743	10.886404043681102	7.909804710229164
43	21.606898656436044	59.649627392806494	9.738610667276989	9.004863283480482
44	20.709662653506122	58.16467059222645	10.775670345250008	10.349996409017411
45	20.938231545350355	57.127282327611205	12.59921221017233	9.335273916866104
46	22.187551488353314	56.55829096856814	11.79280694334422	9.461350599734327
47	23.825850957256712	56.13167821335775	10.374445937539166	9.668024891846377
48	24.30753215136471	55.57329550876378	10.155908997057676	9.963263342813839
49	23.49983265551721	56.05619046169307	10.112750147478403	10.331226735311322
50	22.57899206114555	56.533868263445754	10.410650820832902	10.4764888545758
51	21.68935378608594	56.43936942748069	10.885934634192203	10.985342152241165
52	23.39873526330821	54.14693442409793	11.218625256523904	11.235705056069957
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	11.0
4	22.0
5	50.0
6	78.0
7	180.0
8	282.0
9	4627.0
10	8972.0
11	19158.5
12	29345.0
13	54412.0
14	99028.0
15	118577.0
16	129906.5
17	141236.0
18	153204.5
19	165173.0
20	189498.5
21	213824.0
22	263967.0
23	314110.0
24	393124.5
25	472139.0
26	574861.5
27	677584.0
28	777188.0
29	876792.0
30	940248.0
31	1003704.0
32	1033484.5
33	1063265.0
34	1051270.5
35	1039276.0
36	1009559.5
37	979843.0
38	939369.0
39	860642.5
40	822390.0
41	802359.5
42	782329.0
43	776555.5
44	770782.0
45	794886.5
46	818991.0
47	825289.5
48	831588.0
49	814696.0
50	797804.0
51	747695.0
52	697586.0
53	618420.0
54	539254.0
55	441896.0
56	344538.0
57	269237.5
58	193937.0
59	145594.0
60	97251.0
61	76618.5
62	55986.0
63	44625.0
64	24742.5
65	16221.0
66	15171.5
67	14122.0
68	10715.5
69	7309.0
70	5141.5
71	2974.0
72	1819.5
73	665.0
74	1100.5
75	1536.0
76	1268.0
77	1000.0
78	569.0
79	138.0
80	95.5
81	53.0
82	38.5
83	24.0
84	17.5
85	11.0
86	7.0
87	3.0
88	2.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1.4912353E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.17067363225817
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.89123604914003	35.37749936035016
2	7.391257042395277	6.23388576933962
3	2.341963607436433	2.9628654884348347
4	1.0894937039024137	1.8377873364667525
5	0.664002795600024	1.400072259207782
6	0.44893787923747186	1.1359207671888936
7	0.3319632257198116	0.9799378994819282
8	0.27259896707056647	0.919654565825884
9	0.22267512016044158	0.8451323836478869
>10	2.8932404488072394	28.670069872684977
>50	0.35770433737545754	10.0413514631769
>100	0.0864275951002711	6.198711243673684
>500	0.005723985375486043	1.6706009480565003
>1k	0.0027752426789314677	1.7265106424641377
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.021921423131547383	0.0	0.0	0.0	0.0
12	0.03193996279460391	0.0	0.0	0.0	0.0
13	0.053338329638521836	0.0	0.0	0.0	0.0
14	0.26215178784997917	0.0	0.0	0.0	0.0
15	0.5263958008504761	0.0	0.0	0.0	0.0
16	0.7249157795553793	0.0	0.0	0.0	0.0
17	0.8900272143504113	0.0	0.0	0.0	0.0
18	1.0939118729284372	0.0	0.0	0.0	0.0
19	1.3301924920902826	0.0	0.0	0.0	0.0
20	1.5721328485182722	0.0	0.0	0.0	0.0
21	1.7917360191245473	0.0	0.0	0.0	0.0
22	2.00516310202689	0.0	0.0	0.0	0.0
23	2.211522219196394	0.0	0.0	0.0	0.0
24	2.4470786065753676	0.0	0.0	0.0	0.0
25	2.699191737212766	0.0	0.0	0.0	0.0
26	2.9741919333588736	0.0	0.0	0.0	0.0
27	3.2673582767253433	0.0	0.0	0.0	0.0
28	3.5629051967855108	0.0	0.0	0.0	0.0
29	3.8689937127963643	0.0	0.0	0.0	0.0
30	4.198820937245785	0.0	0.0	0.0	0.0
31	4.527474637972961	0.0	0.0	0.0	0.0
32	4.83970571243854	0.0	0.0	0.0	0.0
33	5.142729655071872	0.0	0.0	0.0	0.0
34	5.441394795308292	0.0	0.0	0.0	0.0
35	5.740301346139003	0.0	0.0	0.0	0.0
36	6.031603463249562	0.0	0.0	0.0	0.0
37	6.319338068244495	0.0	0.0	0.0	0.0
38	6.59895859493133	0.0	0.0	0.0	0.0
39	6.876533837416536	0.0	0.0	0.0	0.0
40	7.156409186397345	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACTCG	20	6.313897E-4	46.000004	45
GTTACTC	20	6.313897E-4	46.000004	35
ACCTTAC	20	6.313897E-4	46.000004	29
CCTTACT	25	3.4191384E-5	46.0	30
CATATCT	110	0.0	46.0	41
CCTAGAC	75	0.0	46.0	31
TTATGTC	90	0.0	46.0	18
CGATTTA	90	0.0	46.0	24
CCGCTAA	55	1.8189894E-12	46.0	46
TAACATC	60	0.0	46.0	18
CTGTTCA	25	3.4191384E-5	46.0	22
CATACTG	65	0.0	46.0	41
CTATTGG	25	3.4191384E-5	46.0	36
CGACTTG	125	0.0	46.0	34
GTACGGT	115	0.0	46.0	36
CTTACGG	65	0.0	46.0	39
CCTATAA	35	1.0206713E-7	45.999996	44
CACGTGT	225	0.0	44.97778	41
CCGGTAA	215	0.0	44.930233	15
TCCGGTA	215	0.0	44.930233	14
>>END_MODULE