FastQCFastQC Report
Fri 17 Jun 2016
SRR1528178_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528178_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14835816
Sequences flagged as poor quality0
Sequence length52
%GC38

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGAC700.046.00000431
GATACGT206.3138956E-446.00000425
CTACGTG206.3138956E-446.00000421
CTAAACT206.3138956E-446.00000414
TAGGGTC206.3138956E-446.00000410
TACGTGA206.3138956E-446.00000423
CCCTAGT501.6370905E-1146.036
CCCTACA850.046.033
TTGCTAC253.4191376E-546.021
CGATTCA750.046.028
CTAGTTC453.110472E-1046.029
TTAAGCG1150.046.023
CATACTA253.4191376E-546.017
TTAACGG850.046.031
CACTTGA950.046.026
ATTTCAC2500.045.0831
ATAACCT1600.044.56250431
GACGTAC1400.044.35714319
CCTAAGG1400.044.35714346
TACGTCA1350.044.29629534
Produced by FastQC (version 0.11.5)