##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528178_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14835816
Sequences flagged as poor quality	0
Sequence length	52
%GC	38
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.883076266246494	31.0	31.0	34.0	27.0	34.0
2	31.58366078414561	31.0	31.0	34.0	28.0	34.0
3	31.76976345621973	31.0	31.0	34.0	30.0	34.0
4	35.511816673919384	37.0	35.0	37.0	33.0	37.0
5	35.32485459512304	37.0	35.0	37.0	33.0	37.0
6	35.41673380149767	37.0	35.0	37.0	33.0	37.0
7	35.35456998118607	37.0	35.0	37.0	32.0	37.0
8	35.32864407323466	37.0	35.0	37.0	32.0	37.0
9	37.44609713412461	39.0	37.0	39.0	34.0	39.0
10	37.75109033436381	39.0	37.0	39.0	35.0	39.0
11	37.80742279359625	39.0	37.0	39.0	35.0	39.0
12	37.69407210226927	39.0	37.0	39.0	35.0	39.0
13	37.58665360907685	39.0	37.0	39.0	35.0	39.0
14	37.975205947552865	40.0	38.0	40.0	33.0	40.0
15	36.98624780733328	39.0	36.0	40.0	31.0	40.0
16	35.8521554190211	38.0	34.0	40.0	30.0	40.0
17	35.35114435228908	38.0	34.0	40.0	27.0	40.0
18	35.32860754002341	38.0	34.0	40.0	26.0	40.0
19	35.69580931712822	38.0	34.0	40.0	27.0	40.0
20	35.88546042900505	38.0	34.0	40.0	30.0	40.0
21	36.06227773383008	38.0	35.0	40.0	30.0	40.0
22	36.04337065113237	38.0	35.0	40.0	30.0	40.0
23	36.12579166525117	38.0	35.0	40.0	30.0	40.0
24	36.22886540248275	38.0	35.0	40.0	30.0	40.0
25	36.297394359703574	39.0	35.0	40.0	31.0	40.0
26	35.98095109834201	38.0	35.0	40.0	30.0	40.0
27	35.69389240200876	38.0	35.0	40.0	27.0	40.0
28	35.425365952233435	38.0	35.0	40.0	27.0	40.0
29	35.20766778180587	37.0	34.0	40.0	27.0	40.0
30	34.85348362368474	36.0	34.0	40.0	26.0	40.0
31	34.65628071957754	35.0	33.0	40.0	25.0	40.0
32	34.49946224730746	35.0	33.0	40.0	25.0	40.0
33	34.21281060644052	35.0	33.0	40.0	24.0	40.0
34	33.914216380143834	35.0	33.0	40.0	23.0	40.0
35	33.50167540497941	35.0	33.0	40.0	21.0	40.0
36	33.301786838014166	35.0	33.0	39.0	21.0	40.0
37	33.112706035178654	35.0	33.0	40.0	21.0	40.0
38	32.81567215446727	35.0	33.0	39.0	18.0	40.0
39	32.32059308365647	35.0	32.0	39.0	16.0	40.0
40	31.74489128201644	35.0	31.0	39.0	15.0	40.0
41	31.022658409891307	35.0	29.0	38.0	10.0	40.0
42	30.257116629108907	35.0	25.0	38.0	9.0	40.0
43	29.43312204734812	35.0	23.0	37.0	8.0	40.0
44	28.506119110671094	34.0	20.0	36.0	8.0	40.0
45	27.85677242155066	33.0	20.0	35.0	8.0	40.0
46	27.228458751443128	33.0	15.0	35.0	8.0	40.0
47	26.691567015929557	33.0	15.0	35.0	8.0	40.0
48	26.20738427869421	33.0	10.0	35.0	8.0	39.0
49	25.74073910056582	33.0	10.0	35.0	8.0	39.0
50	25.385526620173774	33.0	10.0	35.0	8.0	39.0
51	24.99241828019436	32.0	10.0	35.0	8.0	38.0
52	24.130791255432126	31.0	9.0	35.0	8.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	9.0
12	22.0
13	41.0
14	165.0
15	729.0
16	2709.0
17	8072.0
18	19501.0
19	37893.0
20	64606.0
21	99230.0
22	138511.0
23	185432.0
24	246578.0
25	324097.0
26	420979.0
27	531781.0
28	661888.0
29	807491.0
30	934387.0
31	949856.0
32	835832.0
33	780848.0
34	907686.0
35	1171508.0
36	1636872.0
37	2034040.0
38	1820551.0
39	214501.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.05535981303624	23.882575788214144	36.19122803895654	2.8708363597930844
2	29.3518603897487	21.595124932797766	30.66642239294421	18.386592284509327
3	17.664582790727522	22.411109709098575	35.05656176916727	24.86774573100664
4	14.201038891288487	25.70811069643894	33.12516817409976	26.96568223817281
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	100.0	0.0	0.0	0.0
8	0.0	0.0	0.0	100.0
9	0.0	0.0	100.0	0.0
10	0.0	0.0	100.0	0.0
11	99.5492260081953	0.27885894513655335	0.13828022671621162	0.03363481995193254
12	99.26335025993852	0.5512335822984055	0.09812739656517713	0.08728876119790108
13	96.71697869534106	2.168670735738432	0.5273387051982851	0.5870118637222247
14	60.235008306924264	26.951035251448253	5.243297706037875	7.5706587355896025
15	43.495214553752895	34.27870768955344	13.270217155564614	8.95586060112905
16	44.598301839278676	32.56494283833124	14.612927256579619	8.223828065810467
17	30.183462776836812	35.48962187182694	18.933417615856115	15.393497735480139
18	23.577307780037174	45.75163914138595	17.428188648335894	13.24286443024098
19	25.372483724521793	46.672343469344725	17.155598316937876	10.799574489195605
20	18.432393607469923	50.81185962403416	18.954164705197208	11.801582063298708
21	19.124360938420914	52.41089536295138	15.272075361409174	13.192668337218524
22	21.82167802566438	52.35818508398864	16.12693902377867	9.69319786656831
23	16.753712771848882	49.676101402174304	19.278097005247304	14.292088820729509
24	17.91478810467857	52.19067828827211	16.05803819621381	13.836495410835509
25	17.778846812335768	46.55156143753737	21.662030588678103	14.007561161448754
26	19.01525335714598	46.52896072585425	21.30502292560113	13.150762991398654
27	24.034208836237923	47.970681221713726	17.52678787604268	10.46832206600567
28	26.44398528533921	46.60743298514891	16.710789618852107	10.237792110659772
29	30.051343316741054	44.71187833550915	16.02778707959171	9.208991268158083
30	33.54525292036515	45.9421578159233	12.293088563514134	8.219500700197415
31	30.502703727250324	48.714024223541195	11.211705510502421	9.571566538706062
32	28.263541418955317	53.22090810508839	11.814894441937	6.700656034019295
33	26.89619499190338	55.83372023486945	10.935866284672175	6.334218488554995
34	23.785331389928267	57.90170220498825	11.705045411725248	6.607920993358235
35	23.468645068124328	58.45621164349841	11.239941234105357	6.835202054271905
36	19.123909328613944	60.75073322559407	11.913122945175378	8.212234500616617
37	17.834684657722903	62.34573817847296	12.72000138044311	7.099575783361023
38	17.110619328252653	63.17633623927394	12.256447505145655	7.456596927327759
39	17.61448106393339	63.79655153447575	10.862422397258095	7.726545004332758
40	18.186286484006004	63.85650105123979	10.57157894112464	7.385633523629573
41	18.83497341838157	63.42364316192651	10.427515412701263	7.313868006990651
42	20.328062844672647	60.864087287143484	10.887281157976076	7.920568710207784
43	21.681827275291095	59.609137778468	9.709745658749071	8.999289287491838
44	20.657771705985027	58.11694483134598	10.87646274394344	10.348820718725548
45	20.89832470286771	57.10585113754444	12.676977120773133	9.318847038814718
46	22.124849755483623	56.577258709598446	11.812568988453348	9.485322546464582
47	23.933129124815245	56.173977892419266	10.238540300041468	9.654352682724024
48	24.352270208797414	55.636595924349564	10.050670620342016	9.960463246511011
49	23.329063935546248	56.01546959061773	10.341830877384837	10.313635596451183
50	22.59642476018845	56.52257348028582	10.423558771556616	10.457442987969115
51	21.626083796132278	56.45197406061116	10.943927856748829	10.978014286507733
52	23.60961473234772	53.94673269067236	11.288020827435444	11.155631749544481
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	7.5
4	15.0
5	48.0
6	81.0
7	170.5
8	260.0
9	4553.5
10	8847.0
11	18829.5
12	28812.0
13	53510.5
14	97586.5
15	116964.0
16	128107.0
17	139250.0
18	150721.5
19	162193.0
20	186140.5
21	210088.0
22	260298.0
23	310508.0
24	389907.0
25	469306.0
26	571824.0
27	674342.0
28	774126.5
29	873911.0
30	938238.0
31	1002565.0
32	1031174.0
33	1059783.0
34	1048418.0
35	1037053.0
36	1007693.0
37	978333.0
38	936933.5
39	857013.5
40	818493.0
41	799605.5
42	780718.0
43	775049.0
44	769380.0
45	791843.5
46	814307.0
47	821076.5
48	827846.0
49	811098.5
50	794351.0
51	743491.0
52	692631.0
53	612649.0
54	532667.0
55	436602.5
56	340538.0
57	265929.5
58	191321.0
59	143740.5
60	96160.0
61	75429.0
62	54698.0
63	43771.5
64	24384.0
65	15923.0
66	14903.0
67	13883.0
68	10617.0
69	7351.0
70	5248.5
71	3146.0
72	1928.5
73	711.0
74	1111.0
75	1511.0
76	1292.0
77	1073.0
78	596.0
79	119.0
80	84.5
81	50.0
82	40.5
83	31.0
84	18.5
85	6.0
86	4.5
87	3.0
88	1.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1.4835816E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.002158573159996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.62592852094951	36.39097597658774
2	6.9830766506988455	6.005747389237655
3	2.2049655850482277	2.8445483920981314
4	1.075933120659605	1.8506978667487688
5	0.642969797832815	1.3824544602079667
6	0.4381695329938154	1.1305341443836503
7	0.31639155918426476	0.9523863999475761
8	0.2526073111409599	0.86901277203385
9	0.20639163694545917	0.798775731009243
>10	2.821528595673327	28.59952400707354
>50	0.34083947930247355	9.740293906093484
>100	0.08305550749393689	6.121150895763893
>500	0.005532074718754273	1.6553528169980656
>1k	0.0026106273580585875	1.658545241816499
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.022310872553285913	0.0	0.0	0.0	0.0
12	0.03294055412927742	0.0	0.0	0.0	0.0
13	0.053957261265575145	0.0	0.0	0.0	0.0
14	0.26178539825514147	0.0	0.0	0.0	0.0
15	0.5239550018684513	0.0	0.0	0.0	0.0
16	0.7196840402981541	0.0	0.0	0.0	0.0
17	0.8801672924495694	0.0	0.0	0.0	0.0
18	1.0824750050822955	0.0	0.0	0.0	0.0
19	1.3164628086517116	0.0	0.0	0.0	0.0
20	1.556806851743106	0.0	0.0	0.0	0.0
21	1.7749276480646565	0.0	0.0	0.0	0.0
22	1.9872786235688014	0.0	0.0	0.0	0.0
23	2.194722555200199	0.0	0.0	0.0	0.0
24	2.425582792345227	0.0	0.0	0.0	0.0
25	2.674392834206086	0.0	0.0	0.0	0.0
26	2.9449138490258977	0.0	0.0	0.0	0.0
27	3.2354944278090265	0.0	0.0	0.0	0.0
28	3.5289936192252584	0.0	0.0	0.0	0.0
29	3.831134060977839	0.0	0.0	0.0	0.0
30	4.1565290375669255	0.0	0.0	0.0	0.0
31	4.483770896053173	0.0	0.0	0.0	0.0
32	4.7988058088614745	0.0	0.0	0.0	0.0
33	5.096881762351326	0.0	0.0	0.0	0.0
34	5.389167673689132	0.0	0.0	0.0	0.0
35	5.680105496050908	0.0	0.0	0.0	0.0
36	5.969978328121622	0.0	0.0	0.0	0.0
37	6.254829528756625	0.0	0.0	0.0	0.0
38	6.528309598878821	0.0	0.0	0.0	0.0
39	6.805065525212769	0.0	0.0	0.0	0.0
40	7.077541269047823	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGAC	70	0.0	46.000004	31
GATACGT	20	6.3138956E-4	46.000004	25
CTACGTG	20	6.3138956E-4	46.000004	21
CTAAACT	20	6.3138956E-4	46.000004	14
TAGGGTC	20	6.3138956E-4	46.000004	10
TACGTGA	20	6.3138956E-4	46.000004	23
CCCTAGT	50	1.6370905E-11	46.0	36
CCCTACA	85	0.0	46.0	33
TTGCTAC	25	3.4191376E-5	46.0	21
CGATTCA	75	0.0	46.0	28
CTAGTTC	45	3.110472E-10	46.0	29
TTAAGCG	115	0.0	46.0	23
CATACTA	25	3.4191376E-5	46.0	17
TTAACGG	85	0.0	46.0	31
CACTTGA	95	0.0	46.0	26
ATTTCAC	250	0.0	45.08	31
ATAACCT	160	0.0	44.562504	31
GACGTAC	140	0.0	44.357143	19
CCTAAGG	140	0.0	44.357143	46
TACGTCA	135	0.0	44.296295	34
>>END_MODULE