FastQCFastQC Report
Fri 17 Jun 2016
SRR1528177_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528177_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14210195
Sequences flagged as poor quality0
Sequence length59
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATTGA650.053.00000450
CTCACGT2050.050.41463539
CGATTCA850.049.88235528
CTAATTA800.049.687553
TTACAGG3850.049.5584447
CCATTTA750.049.46666750
TCGCTAC1850.048.702751
CGAACTT600.048.58333247
TGCATTA1200.048.58333251
TATTGAC1700.048.32353224
TAACCTA1700.048.32353232
TCACGAG1100.048.18181616
TTTACGA551.0913936E-1148.18181651
TACTCCA1100.048.18181618
TCACCGT1450.047.5172432
ATTAAGT1900.047.42105540
CTACATT1850.047.2702736
CTAATTG900.047.1111121
TCACGTG2200.046.97727240
TGGGCGC124050.046.89036610
Produced by FastQC (version 0.11.5)