##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528177_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14210195
Sequences flagged as poor quality	0
Sequence length	59
%GC	37
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.45403078564369	31.0	30.0	33.0	27.0	34.0
2	31.24367146263651	31.0	31.0	34.0	28.0	34.0
3	31.443366611084507	31.0	31.0	34.0	30.0	34.0
4	35.236356010596616	35.0	35.0	37.0	33.0	37.0
5	34.765097593664265	35.0	35.0	37.0	32.0	37.0
6	35.00105579128225	36.0	35.0	37.0	32.0	37.0
7	34.81458649934079	37.0	35.0	37.0	32.0	37.0
8	34.93169643344092	37.0	35.0	37.0	32.0	37.0
9	37.06983577635634	39.0	37.0	39.0	33.0	39.0
10	37.444285739921234	39.0	37.0	39.0	35.0	39.0
11	37.4090861525827	39.0	37.0	39.0	35.0	39.0
12	37.37220931873208	39.0	37.0	39.0	35.0	39.0
13	37.26527468483015	39.0	37.0	39.0	35.0	39.0
14	37.273965980058684	40.0	37.0	40.0	32.0	40.0
15	36.18188385169943	38.0	35.0	40.0	29.0	40.0
16	34.960960774992884	37.0	33.0	39.0	27.0	40.0
17	34.60944139049464	37.0	33.0	40.0	25.0	40.0
18	34.67747852861977	37.0	33.0	40.0	25.0	40.0
19	35.100307420130406	38.0	33.0	40.0	27.0	40.0
20	35.19554397388635	38.0	33.0	40.0	27.0	40.0
21	35.30726939355864	38.0	34.0	40.0	27.0	40.0
22	35.32497787679902	38.0	34.0	40.0	27.0	40.0
23	35.31222287941861	38.0	34.0	40.0	27.0	40.0
24	35.382328110205385	38.0	34.0	40.0	27.0	40.0
25	35.46372931546682	38.0	34.0	40.0	27.0	40.0
26	35.09976590750514	38.0	33.0	40.0	25.0	40.0
27	34.81106177642179	38.0	33.0	40.0	25.0	40.0
28	34.49328133780008	37.0	33.0	40.0	24.0	40.0
29	34.199528226037714	36.0	33.0	40.0	24.0	40.0
30	33.92563719217083	35.0	33.0	40.0	24.0	40.0
31	33.67586806514618	35.0	33.0	39.0	23.0	40.0
32	33.39060786991312	35.0	32.0	39.0	22.0	40.0
33	32.86423120864985	35.0	31.0	39.0	21.0	40.0
34	32.50038264781025	35.0	31.0	38.0	20.0	40.0
35	32.264676733852	35.0	31.0	38.0	18.0	40.0
36	31.90765235804294	35.0	30.0	38.0	18.0	40.0
37	31.887080296927664	35.0	30.0	38.0	17.0	40.0
38	31.710312420061793	35.0	30.0	38.0	16.0	40.0
39	31.156619877489366	35.0	29.0	38.0	15.0	40.0
40	30.674451617307152	35.0	26.0	38.0	12.0	40.0
41	29.98516607266825	34.0	24.0	37.0	10.0	40.0
42	29.338861148633075	34.0	23.0	37.0	9.0	40.0
43	28.565160154382117	33.0	21.0	36.0	8.0	40.0
44	27.74520272241162	33.0	18.0	35.0	8.0	40.0
45	27.162645832798212	33.0	18.0	35.0	8.0	40.0
46	26.52950328971559	33.0	15.0	35.0	8.0	39.0
47	25.86645714573234	33.0	12.0	35.0	8.0	39.0
48	25.395556640848348	31.0	10.0	35.0	8.0	38.0
49	24.99946024667501	31.0	10.0	35.0	8.0	38.0
50	24.465481860030774	30.0	10.0	35.0	8.0	38.0
51	24.055483193580383	29.0	10.0	35.0	8.0	37.0
52	23.426411741710794	27.0	9.0	35.0	8.0	36.0
53	23.21983132532664	27.0	9.0	35.0	8.0	36.0
54	22.902858687020128	26.0	9.0	35.0	8.0	35.0
55	22.580094009969603	25.0	9.0	35.0	8.0	35.0
56	22.028055843005674	23.0	9.0	34.0	8.0	35.0
57	21.386914254167518	22.0	8.0	33.0	8.0	35.0
58	20.784153137940752	20.0	8.0	33.0	8.0	35.0
59	19.930980398228172	18.0	8.0	33.0	6.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	18.0
12	40.0
13	146.0
14	698.0
15	3122.0
16	11228.0
17	29402.0
18	60865.0
19	105809.0
20	162344.0
21	226964.0
22	303397.0
23	395768.0
24	505538.0
25	628912.0
26	761823.0
27	877931.0
28	899776.0
29	778160.0
30	671847.0
31	691090.0
32	786413.0
33	904757.0
34	1046472.0
35	1181744.0
36	1348598.0
37	1160802.0
38	634027.0
39	32503.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.403413535141496	24.537946171745002	35.155288157551674	2.9033521355618275
2	28.788415641023928	22.238589970088377	30.307536244224657	18.665458144663038
3	17.597949922573193	22.808849561881452	34.70678621933056	24.886414296214795
4	14.235934130390188	26.016441012948803	33.02896265674046	26.71866219992055
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	100.0	0.0	0.0	0.0
8	0.0	0.0	0.0	100.0
9	0.0	0.0	100.0	0.0
10	0.0	0.0	100.0	0.0
11	99.51016857967115	0.30870793820915193	0.14168700711003615	0.039436475009667356
12	99.23416955221234	0.569689578503321	0.1019620068549376	0.09417886242940368
13	96.650095230924	2.2164157493968237	0.5368469609319224	0.5966420587472585
14	59.04031577328812	27.715186174433214	5.486082351438528	7.758415700840136
15	42.75637315321852	34.769389160388016	13.477485706564899	8.996751979828566
16	44.22943527516688	32.47281969037019	14.849212132556943	8.44853290190599
17	29.89941376596169	35.321035355250224	19.059168435056662	15.72038244373142
18	23.448812630650036	45.57088766199197	17.494341210658966	13.485958496699023
19	25.414309937337244	46.20562209033726	17.454911772850405	10.925156199475094
20	18.45943704502296	50.1812536703402	19.442583300229167	11.916725984407673
21	19.284555912146175	51.77324449101508	15.567238873217432	13.374960723621315
22	22.0280439501358	51.639171735503986	16.508126735769636	9.824657578590584
23	16.85914936424166	48.97777264844008	19.759158829277148	14.403919158041113
24	17.880824295514593	51.665420495637115	16.416861274598975	14.03689393424932
25	17.925200885702132	45.746303974013024	22.147627108565366	14.180868031719479
26	19.040702819349065	45.82396652544177	21.744050662218218	13.391279992990949
27	24.172926550269015	47.45825092477619	17.86146495526627	10.507357569688523
28	26.56233781450571	46.05563118591969	17.08905472444256	10.292976275132045
29	30.331997555276335	44.013034303892375	16.280304387096727	9.374663753734556
30	33.90130818049998	45.360053116793964	12.431039827391531	8.307598875314518
31	30.679051202323404	48.15951505239724	11.43296063143398	9.728473113845377
32	28.470671936592	52.83828265551599	12.023628106440482	6.667417301451528
33	26.96271233434868	55.47130071051101	11.29194919563032	6.274037759509985
34	23.93609658417777	57.589104160780344	11.96600750376754	6.50879175127435
35	23.770961622975616	58.259608682357985	11.261471077631235	6.707958617035163
36	19.131644569268754	60.462836716878265	12.231366283150935	8.174152430702042
37	17.8035558273479	62.39740552469547	12.791027850075245	7.008010797881379
38	17.022764290004467	63.38409852926016	12.226503577185253	7.3666336035501265
39	17.453856192684196	64.04661582758013	10.875311704026581	7.624216275709095
40	17.891626399215493	64.39915145429039	10.434381794197757	7.274840352296362
41	18.37856552988893	64.08350483578867	10.407204123518362	7.13072551080404
42	19.77595662832213	61.65172258368023	10.805777119877666	7.766543668119966
43	21.00872648123407	60.502392824306774	9.581093011038906	8.90778768342025
44	19.814893462053124	59.212734237637136	10.634878690967998	10.337493609341744
45	19.900937320001592	58.36971273089496	12.536485248794968	9.192864700308476
46	21.161884126150273	57.87125370200761	11.604274255209024	9.362587916633093
47	22.962119802015383	57.42983118810122	10.067856211684639	9.540192798198758
48	23.216148687614773	57.10227762532464	9.72717826884149	9.954395418219104
49	22.53825510487365	57.37584881840115	9.852088588509869	10.233807488215326
50	21.313669516850403	58.013517759608504	10.21116881225064	10.46164391129045
51	20.5962409382841	57.82553300640843	10.70216137076233	10.876064684545145
52	20.22718196337207	56.99028760689069	11.453340365842973	11.329190063894268
53	19.623249364276845	57.11672499919952	11.21335773365531	12.046667902868329
54	19.160152270957575	56.74986163103321	11.728262701532245	12.361723396476966
55	19.41723530183787	55.324173946944434	12.108004147726332	13.150586603491366
56	19.49268113491757	53.78668624885161	12.836481132032318	13.884151484198492
57	19.52966162674052	51.84106903529473	13.740282944744953	14.888986393219797
58	20.10132865875521	49.56379557071525	14.50518448198635	15.829691288543192
59	21.633242893570426	45.65051359252987	16.173451525471677	16.542791988428025
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	5.0
4	32.5
5	55.0
6	120.5
7	186.0
8	3344.0
9	14106.0
10	21710.0
11	39313.5
12	56917.0
13	68925.0
14	83652.5
15	86372.0
16	84125.0
17	81878.0
18	80934.5
19	87599.0
20	95207.0
21	116745.5
22	138284.0
23	181891.5
24	225499.0
25	299392.0
26	469840.5
27	566396.0
28	673136.5
29	779877.0
30	868590.0
31	1012942.5
32	1068582.0
33	1083474.5
34	1098367.0
35	1071761.5
36	1003056.5
37	960957.0
38	910546.0
39	860135.0
40	820241.0
41	780347.0
42	747747.5
43	689855.0
44	664562.0
45	653119.0
46	641676.0
47	624490.5
48	582640.0
49	557975.0
50	526135.5
51	494296.0
52	443210.5
53	344716.5
54	297308.0
55	245003.5
56	192699.0
57	153245.5
58	90109.0
59	66426.0
60	52664.0
61	38902.0
62	31653.5
63	24405.0
64	18158.0
65	11053.5
66	10196.0
67	8276.5
68	6357.0
69	6347.5
70	4108.0
71	1878.0
72	1327.0
73	776.0
74	1329.0
75	1033.5
76	185.0
77	120.0
78	55.0
79	38.0
80	21.0
81	20.0
82	12.5
83	6.0
84	4.5
85	3.0
86	1.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	1.4210195E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.984787654963135
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.69799441448137	55.921827177531426
2	3.800797449959213	4.635816508105759
3	1.157080390911287	2.1169290581833975
4	0.56649849077953	1.381911606681869
5	0.3604841452011815	1.0992024524037476
6	0.2518154027443622	0.9214145318768371
7	0.19839063689554276	0.8469167604665299
8	0.15232759598447612	0.7431732876083433
9	0.12788369251169435	0.7019063849122452
>10	1.5175865989814366	20.254455521451238
>50	0.13094432823477828	5.276849754579857
>100	0.03378660267726005	3.747984905523454
>500	0.003226052357812281	1.3475158766764868
>1k	0.001184198280094234	1.004096173998793
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.019837869923671	0.0	0.0	0.0	0.0
12	0.029035491771928535	0.0	0.0	0.0	0.0
13	0.04870446886900567	0.0	0.0	0.0	0.0
14	0.23679478008570606	0.0	0.0	0.0	0.0
15	0.4655601137070955	0.0	0.0	0.0	0.0
16	0.6405190076561229	0.0	0.0	0.0	0.0
17	0.7806085701146255	0.0	0.0	0.0	0.0
18	0.9575801035805631	0.0	0.0	0.0	0.0
19	1.1642908489292372	0.0	0.0	0.0	0.0
20	1.3705723250103183	0.0	0.0	0.0	0.0
21	1.5566359223078923	0.0	0.0	0.0	0.0
22	1.7372034655400577	0.0	0.0	0.0	0.0
23	1.916222824528446	0.0	0.0	0.0	0.0
24	2.118303091548005	0.0	0.0	0.0	0.0
25	2.329186897153769	0.0	0.0	0.0	0.0
26	2.5614497197258728	0.0	0.0	0.0	0.0
27	2.8082513997872653	0.0	0.0	0.0	0.0
28	3.0643281109091043	0.0	0.0	0.0	0.0
29	3.329342067438202	0.0	0.0	0.0	0.0
30	3.6078674500948087	0.0	0.0	0.0	0.0
31	3.89150184075588	0.0	0.0	0.0	0.0
32	4.1607170063465	0.0	0.0	0.0	0.0
33	4.423591653738741	0.0	0.0	0.0	0.0
34	4.6831940026157275	0.0	0.0	0.0	0.0
35	4.943338215978035	0.0	0.0	0.0	0.0
36	5.1982749005203654	0.0	0.0	0.0	0.0
37	5.45605461431036	0.0	0.0	0.0	0.0
38	5.702835182768428	0.0	0.0	0.0	0.0
39	5.949904276471927	0.0	0.0	0.0	7.037201108077687E-6
40	6.1974730114541	0.0	0.0	0.0	7.037201108077687E-6
41	6.449622964357632	0.0	0.0	0.0	7.037201108077687E-6
42	6.711470180388095	0.0	0.0	0.0	7.037201108077687E-6
43	6.990825952775454	0.0	0.0	0.0	7.037201108077687E-6
44	7.278119688012726	0.0	7.037201108077687E-6	0.0	7.037201108077687E-6
45	7.5746954915115525	0.0	7.037201108077687E-6	0.0	7.037201108077687E-6
46	7.878231086906267	0.0	7.037201108077687E-6	0.0	2.111160332423306E-5
47	8.187269773567499	0.0	7.037201108077687E-6	0.0	2.111160332423306E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCATTGA	65	0.0	53.000004	50
CTCACGT	205	0.0	50.414635	39
CGATTCA	85	0.0	49.882355	28
CTAATTA	80	0.0	49.6875	53
TTACAGG	385	0.0	49.55844	47
CCATTTA	75	0.0	49.466667	50
TCGCTAC	185	0.0	48.7027	51
CGAACTT	60	0.0	48.583332	47
TGCATTA	120	0.0	48.583332	51
TATTGAC	170	0.0	48.323532	24
TAACCTA	170	0.0	48.323532	32
TCACGAG	110	0.0	48.181816	16
TTTACGA	55	1.0913936E-11	48.181816	51
TACTCCA	110	0.0	48.181816	18
TCACCGT	145	0.0	47.51724	32
ATTAAGT	190	0.0	47.421055	40
CTACATT	185	0.0	47.27027	36
CTAATTG	90	0.0	47.11111	21
TCACGTG	220	0.0	46.977272	40
TGGGCGC	12405	0.0	46.890366	10
>>END_MODULE