FastQCFastQC Report
Fri 17 Jun 2016
SRR1528171_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528171_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences569712
Sequences flagged as poor quality0
Sequence length59
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTAGACACTGGGGTGACACTGGGAGGGTTTCAGAGCACCACTTTCCAAAGCACAAAAC26510.4653228297806611No Hit
TTTTGACACTGGGCACTGACACTGGGGCTTTTTTTCCGACATCTTAGCGAGGCTGCTGT25620.4497009015081304No Hit
TTAGGACACTGGGCTCGGAACTTGCTGAAGTCCATAGCGAAGCAGAGAGAAGCCCAGCA25460.44689246496475415No Hit
GGTGGACACTGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAATGA23900.41951020866683514No Hit
GCACGACACTGGGGCTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCCTCTCGCAA22860.40125537113488924No Hit
ATTCGACACTGGGCTCTTTCCCCTTCTTCTGCCGGCCTCTCCTGCCGGCCGGCTGTCCC20740.3640435869351532No Hit
GAATGACACTGAGGCTGCTTTCACAAGCAGTTTAAATAAAATGAAATCAAACTTCATAG19720.3461398039711293No Hit
ACATGACACTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAG19520.3426292582919089No Hit
GACTGACACTGGGGCTTTGATCATTGAACATCAGCTCCCCGTAGATGTGGGATTCGAAG18840.3306934029825596No Hit
TTAAGACACTGGGATACGCTTCTGCATTTTTTGATAGCGGCGGTAGAAACTCCAGAGCA16780.2945347824865897No Hit
GTGTGACACTGGGCAGCCTGGGCTGCTTGAGACTATGTCTCAAAACAAAACAAAAAAAC16490.28944449125172017No Hit
CCGTGACACTGGGGGAAGGAGGGGGGATTGTTAGCAGCATAGGACTGAGGAGTTGGAAC16170.28382761816496754No Hit
GTCAGACACTGGGAGATCAAGCGAACTGCCCGGACTTCCTGTAAAGGGGGCGGGGGGGG16120.28294998174516245No Hit
ATGCGACACTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGATTGCTCTTCT16100.28259892717724044No Hit
GTCAGACACTGGGGCCCTTCAGCCTAGAGCGCGGAGGAGGACGGTAGCTCGGTCACGGC15790.27715758137444885No Hit
CCTCGACACTGGGGCTGGAGAGATGGCTCAGGGGTTAAGAACACTGACTGCTCTGCCAG15630.27434914483107253No Hit
TAATGACACTGGGGGCTGGAGAAATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCA14980.2629398713736063No Hit
ATGGGACACTGGGGCAAATATGAAATTAAAAGAGGACATGTTTAGCAAACTATGAATAC14910.26171118038587915No Hit
AGTAGACACTGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTCTGTCC14740.25872721655854186No Hit
TTTTGACACTGGGTACCAGCTACTCGGAAGGCTGAGATGGAAGAATCACCTGAGCCCAG14330.25153059791614013No Hit
AAATGACACTGGGATCTTACCTTAGTTGAGCCCATCCTGAAGAGGCGCCTCTTTGGACA14290.250828488780296No Hit
AACAGACACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT14200.24924874322464685No Hit
CTCGGACACTGGGGGCCCTTTGCATTGCACCAGAGGATCCCTGGAGTGACCAGAGGCCA14080.2471424158171146No Hit
TCGTGACACTGGGCCCTTCCGCCTCTCTCTTTCCGGTGGAGGAGTCTGGAGACGACGAT13900.24398292470581626No Hit
TCGTGACACTGGGAGTGGCAGTGTGCACACAGTGAAGGCAGATCCTCAGGGTCTGCCCT13720.2408234335945179No Hit
GCCTGACACTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC13310.23362681495211612No Hit
CTTTGACACTGGGCTTTCGGCCGCCGCAGCCATCTTCCAGTAACTCGCCAAAATGACGA12960.22748336001348052No Hit
GCGAGACACTGGGGGGCTGGAGTCCTGTGCCTGGGGGGGGTCTCCTGCTGCTTAGGTCC12880.22607914174179233No Hit
GTGTGACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTC12810.2248504507540652No Hit
GCACGACACTGGGGAGGAGTTACTACTTTAAAATTAGTTGAAATGGGCCAGGCATGGTG12790.2244993961861432No Hit
ATATGACACTGGGACTCTCCATAGTGCCAAGGCTACACAGAAACCCTGCCTCAAAAAAA12630.22169095964276686No Hit
GGTAGACACTGGGGGCGAGAGTCCATACTAATTATACTTTGTTCTATGACCACGATGTT12420.21800488667958548No Hit
TCTCGACACTGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC12320.2162496138399753No Hit
ATTTGACACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTT12290.2157230319880922No Hit
AAATGACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTC12100.21238801359283288No Hit
ATCTGACACTGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTT12080.21203695902491082No Hit
TATTGACACTGGGAAAGGAAGATGCAGCCAACCGAAGGCTGGCCAAGCTCTACAAGGTC12060.21168590445698882No Hit
ATTCGACACTGGGGAGCACTTACTAAGAGACATCAAGGACACTACAGTGGGGACTCTCT11810.20729772235796332No Hit
GTATGACACTGGGTATCAACGCAGAGTTGTTGACACTGGGGGCTGAAGAGATGGCTCAG11660.20466481309854806No Hit
TTGCGACACTGGGATCGCGTATAGGGACGCCCCCTGCTCGCGTTACTGCCAAGCGAGCG11500.20185637655517172No Hit
CCAAGACACTGGGGATGTTACAACAGCTTGGGGCGGAGATTACTGGCGGCTGCTGACAC11460.20115426741932765No Hit
GATTGACACTGGGTACCAAGCAACCATGGCAGGCACTAGAGGGAGAGTAAGGCTATAGA11440.2008032128514056No Hit
GAGGGACACTGGGATACATGGCCAGGGCCAGGTTGAAAATGTTGATCGTTGCCAGAGTG11250.19746819445614627No Hit
CACAGACACTGGGATTTTTGTGTTACTGCATAGCCTGGGCCACAGGCTTAAAACAGACA11240.19729266717218524No Hit
ATCTGACACTGGGACCAACATGGAGAAACCCTGTCTCTACTAAAAATACAAAATTAGCC11170.19606397618445812No Hit
TCAAGACACTGGGGGCCTTTTGTGACTGGCGGTGAACCACAGCTGAAGAAGATGAAGAA11100.194835285196731No Hit
ATACGACACTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAG10870.19079815766562755No Hit
CTTTGACACTGGGAGGCCGAGGCCAAGATGGCGGCGCTGGCGGCTCCTGGCCTGCTCTC10730.1883407756901733No Hit
ATGAGACACTGGGAAGCAGTAGTATCAACGCAGAGTTAATGACACTGGGGGCTGGAGAA10630.18658550285056308No Hit
TTTAGACACTGGGGCCTTCCGTGGCCGGTGTAGCCATGGCGGCCATGAGCCTGCTGCAG10610.18623444828264105No Hit
GGTAGACACTGGGGTTAGACACTGGGGATATATCCGGCGTGGGCGGAGCAGGATCCGTC10510.18447917544303088No Hit
CATAGACACTGGGTCGAGATATTTGAATCAAGATAAAAAAGCTTATAGCCATATAAACC10340.18149521161569354No Hit
TAGCGACACTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC10310.1809686297638105No Hit
TAGTGACACTGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG10240.17973993877608335No Hit
GTTAGACACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT10220.17938888420816132No Hit
CTTAGACACTGGGCTGGAAGATGGCTCAACGGGTAAGAGCACTGACTGCTCTTGCAAAG10180.17868677507231723No Hit
CAGTGACACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT10160.1783357205043952No Hit
TAATGACACTGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTC10110.17745808408459013No Hit
ACATGACACTGGGGCTCTTCAACGAGGCGGCCGAGCGGCAGACGCCAACATGCAGATCT10080.17693150223270707No Hit
GTTCGACACTGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTT9980.17517622939309685No Hit
ACATGACACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT9950.1746496475412138No Hit
TTGGGACACTGGGGCGCTTTGCCTCCTTCCCCGACTACCTGGTCATCCAGATCAAGAAG9860.17306990198556466No Hit
TTTAGACACTGGGGCCCAAATTATATAAAGATGGACAATATCTTGACAAACAAATCGAA9670.16973488359030528No Hit
GCTGGACACTGGGATATCCAGGTCGTCTACCTTCACAACAACAACATCTCCGCAGTTGG9650.16938382902238325No Hit
AATGGACACTGGGGAAGGTGGGAGATGCCATTCCCTCAGTGGAGGTATTTGAAGGGGAA9630.16903277445446122No Hit
GCTCGACACTGGGAGCTCCGCTGCCCCTCCTCGCCATCTCACCCAAGGCGTGATGGCCC9550.16762855618277303No Hit
CTTTGACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTC9480.16639986519504593No Hit
GTGCGACACTGGGGTAGAAGGGGTGTCCTCCAAGGAAATGGCAACTCAGCTCGCCTTCA9460.1660488106271239No Hit
TTGGGACACTGGGGCTGGAAGATGGCTCAACGGGTAAGAGCACTGACTGCTCTTGCAAA9410.16517117420731878No Hit
CATTGACACTGGATTTCCTTTCCTGTTGGGTTTGAGGCGTTGATAACCACCGAGAGATT9400.16499564692335777No Hit
CGGGGACACTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCT9240.16218721037998146No Hit
CAAAGACACTGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTG9170.16095851939225433No Hit
TTAAGACACTGGGACTGCCCCTCCAGGATGTCTATAAAATTGGAGGCATTGGCACTGTC9140.16043193754037127No Hit
TCTCGACACTGGGAGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTT9090.15955430112056618No Hit
CTTAGACACTGGGGCTTCCACAGGAGGCCTACACGCCGCCGTCTGTGCCGCCGCCATGT9040.15867666470076108No Hit
TACTGACACTGGGGTCGTCTCTGGCGCAATCGGTTAGCGCGTTCGGCTGTTAACCGAAA9010.15815008284887802No Hit
AACAGACACTGGGAGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT9000.15797455556491702No Hit
AACTGACACTGGGTAAAAAACAAAATGAAGACTTCCCCAGGCTAAGTGTGGCAATAGCT8990.157799028280956No Hit
TTACGACACTGGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACCACTTTT8970.15744797371303396No Hit
TTTAGACACTGGGGGGAGTTCTTAACTGTGCTATGGAGTAGAAGCAGGAGGTTTTCAAC8930.15674586457718986No Hit
TATGGACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC8920.15657033729322886No Hit
GCTTGACACTGGGGAGGGCGGGGCAAGAGGCCCAACGAGGACAGACTCCAACTCTGACT8900.1562192827253068No Hit
AGCCGACACTGGGGAGGTCACGGCTTCCGCCGGCGCCAGTGGCCTAGCTCCCTGCGGCT8800.15446400988569664No Hit
ACTAGACACTGGGAGTGATCGACACTGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA8710.15288426433004745No Hit
TCGAGACACTGGGGAGGACACTGGGGGACTGAGAGAGAGTTGTCTATTTTCTGAGACCC8640.15165557334232033No Hit
CTAAGACACTGGGGACACTGGCAACACAGAGTTCTCGACACTGGGAGTCAATCCATAAA8500.14919819136686605No Hit
AATTGACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC8360.1467408093914118No Hit
GTGGGACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTC8330.14621422753952873No Hit
TTCCGACACTGGGGTCTGTCTTGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGA8270.14516106383576263No Hit
GGATGACACTGGGGGGGAGACGGCGGCTGAGCCGCGGCAGGAGAGTACTGAGAAGCGTC8180.14358131828011347No Hit
GTTAGACACTGGGGGCTTGGACTCTGATCTCAACTGCAAGATTGCCCAGCTGCCCTTGA8130.14270368186030835No Hit
TATTGACACTGGGGAAAAACTCTGGGATTGAAAACGGAGCCTTCCAGGGACTGAAGAGT8110.14235262729238632No Hit
ATTTGACACTGGGGCTCTTTCTCCAGGGACACGGATTGGACCACGGACGGGGCGTGATG8080.14182604544050326No Hit
TGTGGACACTGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGAC8020.14077288173673716No Hit
TTTTGACACTGGGATGGCCCAAAAACTATAGTATAAGTTTGAAATTTCGGTTGGGGTGA7930.139193136181088No Hit
AACAGACACTGGGAGTCTGCATCCTAGCCTCTCCCTAGACTAGTGCGGTTTGTAGTGCC7910.13884208161316594No Hit
TTTAGACACTGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTT7870.13813997247732188No Hit
CAATGACACTGGGGGCAGTACTGAAAGCAGGCCGGAGGGAAACCAGCTCGCCGGGGTTG7820.13726233605751678No Hit
TTCTGACACTGGGAAGATCACCAAGGTTGATGCACCCAGCCTGAAAGGACTGATTAATT7650.13427837223017944No Hit
GGCAGACACTGGGATGTTCTGCTCTCTTACAGGGAGCTACCATACCTCACAGTTCAAAG7620.1337517903782964No Hit
CCACGACACTGGGGAGAGCTGGAGAGATGGCTCAGGGGTTAAGAACACTGACTGCTCTG7570.1328741539584913No Hit
CCTCGACACTGGGGCTCTTCCTGTAAGCGGCCAGAGGTCACCTGCCAAGATGGTTCGCT7520.13199651753868621No Hit
AGTTGACACTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAG7450.13076782655095906No Hit
ATTGGACACTGGGGTGAACGAGGCTCCGGCACCATGTCTGGTTTGTTTGGCCCACTCTC7390.12971466284719296No Hit
CTTCGACACTGGGGGGCGTGGCCGAAATAGCTCAGTTGGGAGAGCGTTAGACTGAAGAT7370.12936360827927093No Hit
AGCCGACACTGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACAACT7340.12883702642738787No Hit
TGGCGACACTGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGAC7320.12848597185946584No Hit
TGTTGACACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTT7150.12550200803212852No Hit
TTGAGACACTGGGGGTGCCTGTGGTCTGCAGGCCTCACCGGGTGTTGTGGCCGCTGCTG7150.12550200803212852No Hit
TATTGACACTGGGGGGGCGGAGGCGCTCAGACCTGATCATAGCACAACCCTGCACGGCT7090.1244488443283624No Hit
CTTTGACACTGGGGCTGTGGCCACCGATTATGAAAACTATGCCCTCGTGTACTCCTGCA7070.12409778976044036No Hit
TAATGACACTGGGGATTAGGCGGCGGCGGCTGGCGTGGGGCAGCTTAGATGCGCCACGG7040.1235712079085573No Hit
CTTGGACACTGGGGCTCTTTTCCTCCTCTCCAGCTCCGGCGCCGTAGCCATCATGAATG7040.1235712079085573No Hit
ATCAGACACTGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCC6930.12164040778498611No Hit
GCTAGACACTGGGTAATTATAGTAAAAATAAAGCTTATCTCTTAGTTTCTATATAAATA6870.12058724408121999No Hit
TCCAGACACTGGGATCCGTTTTTGACATTCGAATCGTAGTATGGTAATAACACTAAGTC6850.12023618951329795No Hit
ATCAGACACTGGGAACCAGAAAGCTCTCTCTCCAGCTCCTAGTAAAGGGATTTCCTGTT6830.1198851349453759No Hit
AGTGGACACTGGGGTCTCCGGCGGTGGCTGTGGCGGCACGTCCTAAAGTAATGGCGGCG6750.11848091667368775No Hit
GCTGGACACTGGGACTTGGAGGATTCTGGGTGGAAACTTCAGTCATCTGATCTGAGAGG6610.1160235346982335No Hit
AGTGGACACTGGGTGCCTGAAAAAATGCCCAGAACTCTCCAGGAACTTCGTGTCCATGA6520.11444378914258432No Hit
AGCCGACACTGGGGGGCCCAGGAAGGATCCAGTCTACCTGCCTCACTTCTGAGTGCCTG6510.11426826185862331No Hit
TCTTGACACTGGGCGTGAGAACGCGTATAACAGTTGGTGCCGAAACCCGGGACGAGAAA6480.11374168000674026No Hit
CTCTGACACTGGGGCTTTCTGCCATCTTCCCTCGCCGCCACCATGGTGCGAATGAATGT6390.11216193445109109No Hit
AGCCGACACTGGGAGAGTATTATTTGCCTCTTAAGTTTGTTATAATATGCACGATTGCT6340.11128429803128599No Hit
ATTCGACACTGGGGAAGCAATGGTATCAACGCAGAGTTTAAGACACTGGGATACGCTTC6330.11110877074732496No Hit
ATATGACACTGGGACTTCTAACTAAAAGAATTACAGCTAGAAACCCCGAAACCAAACGA6330.11110877074732496No Hit
ATCGGACACTGGGGGTTCCAAGACTACACAGTGGGGAAGGACCATCTCTTCAACAAATG6300.11058218889544191No Hit
TCTCGACACTGGGGCCTGCTGAAAGCCATTTTGTTTTTCAGGACGCTTTGCAAGGGTAG6280.11023113432751988No Hit
TGTGGACACTGGGATCTATCACCTTCAGTATCCCTGTACCAAGTCAACAGAGGTAGAGG6200.10882691605583172No Hit
TCTAGACACTGGGGGGTAGAAGCTCGTGGAGCCCTGGTGGTTTAGCTTTTGGAGTCGTC6110.10724717050018255No Hit
GCCTGACACTGGGATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCA6030.10584295222849441No Hit
CCTTGACACTGGGAGTCGCAGCCTCCGGGACCATCTCCTCGCTGCCTTCAGCTCCTAGG6030.10584295222849441No Hit
AGCTGACACTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC5970.1047897885247283No Hit
TGCTGACACTGGGGAATTTTGCCCTTGAAGCTGCTAACTACCAGGACACTATTGGCCGC5920.10391215210492318No Hit
TCAAGACACTGGGGGAATTCAGCTAGGATAAATTGACCCGTATGAACTGTTGCCGGCTC5920.10391215210492318No Hit
ATTGGACACTGGGAACATAACATTCTTCCCTCAACATTTCCTGGGCCTTTCAGGAATAC5910.10373662482096217No Hit
GAGCGACACTGGGGAGTCTTGGCCAGCCAAAGGGGACTTGAAGGAGACGGGCGACACGG5860.10285898840115706No Hit
TGATGACACTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCT5800.10180582469739097No Hit
CAAGGACACTGGGGCTTTTTTTTTCTCCACAGGGTCGTGGTGGCAGCCGCTGTGGTGCT5800.10180582469739097No Hit
TACGGACACTGGGGCTCTTTTACCGAGACCCGCCAACATGGGCCGCGTTCGCACCAAGA5750.10092818827758586No Hit
CACTGACACTGGGGGGCCTGGCTTCCCGGCGCGGATTGTGAGGGGTTCTCTCCTCTACG5750.10092818827758586No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTT1500.053.00000451
ACTATGC750.053.00000436
TTGGCCA750.053.00000419
TCTTGGG750.053.00000419
ACAAATC750.053.00000450
GCCTTAC750.053.00000432
GTTCTCA750.053.00000430
ACGCCCC1500.053.00000427
ATGGCTT750.053.00000418
ATGGCCT750.053.00000429
GTCTCGC750.053.00000435
ACACATT750.053.00000440
GGATCTC750.053.00000413
CGCGCAG750.053.00000439
CGATTAT750.053.00000425
GGCCACT750.053.00000423
GAGACAT1500.053.00000427
TGTCTTG750.053.00000417
TACTGCC1500.053.00000444
TCTCGCG750.053.00000436
Produced by FastQC (version 0.11.5)