FastQCFastQC Report
Fri 17 Jun 2016
SRR1528170_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528170_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1306308
Sequences flagged as poor quality0
Sequence length52
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCACAATAGGGGCGGCACCGCCGCCTACGCCGGGCACGTCGCCAAGTACG26240.20087146369768843No Hit
CTTGACAATAGGGAGCTCGGGCTGCTCGTCCGCTCGGCCTCTCCCCCCAACC26230.20079491207280367No Hit
GTCAACAATAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTTTTC24370.18655630984423277No Hit
AATCACAATAGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTG24260.18571424197050004No Hit
CAGGACAATAGGGATGAACAGTTGCCCATACTGGGCAACCGCCCTAGAGAGC22750.17415494661289682No Hit
TATCACAATAGGGGCCCCTTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGA22200.16994460724423338No Hit
TTCGACAATAGGGGCCTTGGCCTGGCCTTAGCAGAGAGTATCTGTTGCAGTC21740.16642323249953303No Hit
GTTCACAATAGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAA21710.16619357762487866No Hit
TATGACAATAGGGCTTTTCTGCCTCCTCTGCCGCCATGGCGCCTGTGAAAAA20700.1584618635115149No Hit
GGTCACAATAGGGATTGATACCACATAGAAAGCATGCAGCCATAGACTTCTG20330.15562945339077766No Hit
GTGGACAATAGGGGAGTTTTCACAATAGGGGAGCCCACTTGGGTGGATTTTT20060.15356255951888836No Hit
GAGAACAATAGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTG19660.15050049452349676No Hit
GGTGACAATAGGGGATGAAAGCGTTGAAGGCTAAGTTCATTAAAATGACTTC19590.14996463314930322No Hit
TGTTACAATAGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGC19180.14682601652902685No Hit
CCGGACAATAGGGGGGTGGCTCACGCCTGTAATCCCAGCACTTTGGTTGGCC18610.14246257391059383No Hit
AGCCACAATAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCG18140.13886464754100872No Hit
TCTTACAATAGGGGCCTTTCCAGCCTCCGCCTCGGACCCTGCAGCCGCCGCG16930.12960190092994914No Hit
CCGCACAATAGGGGGGTGGTGGTGGCGTACGCCTTTAGTCCCAGCACTTGGG16750.12822397168202293No Hit
CTAGACAATAGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGC16740.1281474200571381No Hit
TTGTACAATAGGGGTCTGACCAGAGGCTCCTTGGGGCTCCCTGGACAGAGTC16430.12577431968570965No Hit
CTAGACAATAGGGCGAGCAGGAGATGGCCACTGCCGCATCCTCTTCCTCCCT16360.12523845831151612No Hit
GCGCACAATAGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTG16150.12363087418893552No Hit
GATGACAATAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCG15690.1201094994442352No Hit
TTTGACAATAGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGCT15480.11850191532165463No Hit
GACTACAATAGGGCTTTCGGCCGCCGCAGCCATCTTCCAGTAACTCGCCAAA15430.11811915719723066No Hit
TGCTACAATAGGGACACGCCTGTAACCCCAGCACTTTGGGAGGTGGGTAGAT15250.11674122794930444No Hit
ACGTACAATAGGGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTC15210.1164350214497653No Hit
TAACACAATAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCC14800.11329640482948891No Hit
CTTAACAATAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCG14620.11191847558156269No Hit
TGTTACAATAGGGCTTCCTGTCCCGGCCGCCATCGGAGAGCATCAGCCATGG14380.11008123658432774No Hit
ATTAACAATAGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAA14250.10908606546082547No Hit
ATTAACAATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT13980.10701917158893615No Hit
CAATACAATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGAC13930.1066364134645122No Hit
TGTGACAATAGGGGGGTGGTGGTGGCGTACGCCTTTAGTCCCAGCACTTGGG13900.10640675858985783No Hit
AAGAACAATAGGGACAAAGTGAGACTTCATCTCTACAAAAAATTCAAAAAAT13850.10602400046543388No Hit
GATTACAATAGGGGGACCCTCCCAAACTGCTAGGGCTCTAGGAGGAGACCTA13720.10502882934193161No Hit
GTGGACAATAGGGGGTTCCCGGCAGCCCCCGCGGCGGCCGCTGAAGGTGAGC13540.10365090009400539No Hit
GTTTACAATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT13520.10349779684423581No Hit
GTCTACAATAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCCTCCT13400.10257917734561833No Hit
GCCAACAATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACT13340.10211986759630959No Hit
CTGGACAATAGGGGAGTTACAACAATAGGGGATCTTTCCATCGGTGACCAGC13210.10112469647280733No Hit
GCATACAATAGAGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCG13140.10058883509861381No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACTG206.3117716E-446.00000424
ATCCTTT551.8189894E-1246.00000418
CTTGTCC800.046.00000426
GCCCCGG1050.046.00000423
ATTTAAG551.8189894E-1246.00000415
ACAACCG206.3117716E-446.00000424
ATCCTAT1100.046.00000440
ATCCTAG405.6115823E-946.00000414
CTATGTT1100.046.00000439
GTGCACG405.6115823E-946.00000428
CTAGAGG405.6115823E-946.00000445
CATGGAA1050.046.00000429
CTATGGC206.3117716E-446.00000437
TAATAAT206.3117716E-446.00000425
CTGTCGA800.046.00000437
TCGTTCT405.6115823E-946.00000416
CTGTCAT551.8189894E-1246.00000442
GAAGCTA206.3117716E-446.00000413
ACCGGTA405.6115823E-946.00000446
GATGTTT405.6115823E-946.00000438
Produced by FastQC (version 0.11.5)