##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528170_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1306308
Sequences flagged as poor quality	0
Sequence length	52
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.888355579235526	31.0	31.0	34.0	28.0	34.0
2	31.674409098007516	31.0	31.0	34.0	30.0	34.0
3	31.93608704838369	31.0	31.0	34.0	30.0	34.0
4	35.6020930745276	37.0	35.0	37.0	33.0	37.0
5	35.47905088233403	37.0	35.0	37.0	33.0	37.0
6	35.761240840598084	37.0	35.0	37.0	33.0	37.0
7	35.60369300348769	37.0	35.0	37.0	33.0	37.0
8	35.54704327004045	37.0	35.0	37.0	33.0	37.0
9	37.36661951086574	39.0	37.0	39.0	34.0	39.0
10	36.857995204806215	39.0	37.0	39.0	33.0	39.0
11	37.46248664174146	39.0	37.0	39.0	35.0	39.0
12	37.52186926819709	39.0	37.0	39.0	35.0	39.0
13	37.444601885619626	39.0	37.0	39.0	35.0	39.0
14	37.84211763228886	40.0	38.0	40.0	33.0	40.0
15	36.969297439807455	39.0	36.0	40.0	31.0	40.0
16	36.382200063078535	38.0	35.0	40.0	31.0	40.0
17	35.999802496807796	38.0	35.0	40.0	30.0	40.0
18	36.16907038768805	38.0	35.0	40.0	30.0	40.0
19	36.39906897913815	39.0	35.0	40.0	31.0	40.0
20	36.516025317153385	39.0	35.0	40.0	31.0	40.0
21	36.53027769867443	39.0	35.0	40.0	31.0	40.0
22	36.63608735459019	39.0	35.0	40.0	31.0	40.0
23	36.63938060549273	39.0	35.0	40.0	31.0	40.0
24	36.6490368274557	39.0	35.0	40.0	31.0	40.0
25	36.76423171258233	39.0	35.0	40.0	31.0	40.0
26	36.60718146103369	39.0	35.0	40.0	31.0	40.0
27	36.394293688777836	39.0	35.0	40.0	31.0	40.0
28	36.139611791399886	38.0	35.0	40.0	30.0	40.0
29	36.02293103923424	38.0	35.0	40.0	30.0	40.0
30	35.87507081025302	38.0	35.0	40.0	30.0	40.0
31	35.80719707756517	38.0	35.0	40.0	30.0	40.0
32	35.74952461440947	38.0	35.0	40.0	30.0	40.0
33	35.53961010726414	38.0	35.0	40.0	30.0	40.0
34	35.290510354372785	38.0	34.0	40.0	28.0	40.0
35	35.172706589870074	37.0	34.0	40.0	27.0	40.0
36	34.97835043496633	37.0	34.0	40.0	26.0	40.0
37	34.73208003013072	37.0	34.0	40.0	25.0	40.0
38	34.31150310646494	36.0	33.0	40.0	23.0	40.0
39	33.98715234079559	36.0	33.0	40.0	22.0	40.0
40	33.62178521451296	35.0	33.0	40.0	21.0	40.0
41	33.066942099413005	35.0	33.0	40.0	18.0	40.0
42	32.4638224675957	35.0	32.0	39.0	15.0	40.0
43	31.845403228028918	35.0	31.0	39.0	10.0	40.0
44	31.212203400729383	35.0	30.0	39.0	10.0	40.0
45	30.802825979784245	35.0	29.0	39.0	9.0	40.0
46	30.351306123823786	35.0	26.0	38.0	8.0	40.0
47	29.8059140723321	35.0	24.0	38.0	8.0	40.0
48	29.411367763192143	35.0	23.0	38.0	8.0	40.0
49	29.129964755631903	35.0	23.0	38.0	8.0	40.0
50	28.805271038683067	35.0	20.0	37.0	8.0	40.0
51	28.41084874317542	34.0	20.0	37.0	8.0	40.0
52	27.541166401798044	33.0	17.0	36.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	4.0
14	10.0
15	22.0
16	102.0
17	327.0
18	803.0
19	1673.0
20	2874.0
21	4823.0
22	6980.0
23	10189.0
24	14074.0
25	18830.0
26	24709.0
27	32592.0
28	41569.0
29	52234.0
30	62928.0
31	67194.0
32	64724.0
33	67215.0
34	87715.0
35	130911.0
36	127672.0
37	191454.0
38	241622.0
39	53057.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.157105368718554	21.70514151333376	35.92590721330651	8.211845904641171
2	20.193246921859163	23.75672506024613	36.51190990179958	19.53811811609513
3	21.48658662428769	23.20999335531896	35.734375047844765	19.56904497254859
4	20.376281856958695	24.921764239367743	32.68540038030847	22.016553523365086
5	0.0	100.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	0.0	100.0	0.0	0.0
8	0.0	100.0	0.0	0.0
9	0.0	0.0	100.0	0.0
10	0.0	100.0	0.0	0.0
11	99.29350505393828	0.6078964532101159	0.06208336778156453	0.036515125070044736
12	98.71921476405258	1.077311017003647	0.11505709220183907	0.08841712674193222
13	97.04686796681946	2.0048105041077604	0.5876102726156465	0.36071125645712954
14	58.58932196694807	28.000747143858874	6.545240479274413	6.864690409918641
15	36.36378250764751	32.185212063311255	17.3806636719671	14.070341757074138
16	39.61707346200131	25.584471655995372	19.46248511070896	15.335969771294366
17	23.047321152438784	28.551229878405397	24.070203964149343	24.331245005006476
18	20.571335397165143	38.976412913340496	25.572759257387993	14.879492432106364
19	26.00650076398522	37.77539447052303	25.14873980715115	11.069364958340605
20	20.706831773211217	38.00818796179768	26.725550176528046	14.559430088463058
21	26.163737801498577	37.31440058546683	16.587971596285104	19.933890016749494
22	24.025191608717087	44.86461079622876	15.871754593863011	15.238443001191143
23	18.629450328712675	44.23550954292556	18.876865180340317	18.25817494802145
24	16.810277514950535	48.769356078352125	16.006791660159777	18.41357474653757
25	20.96504040394761	42.625781974848195	18.59706899138641	17.81210862981778
26	18.43676988887766	42.28420862461227	17.39421330957171	21.884808176938364
27	20.002403721021384	48.941979992467324	13.572603092073232	17.483013194438065
28	20.84095022000937	45.22624067218451	14.75907672616259	19.173732381643532
29	22.121735455956788	43.0435241918445	13.804707618723915	21.0300327334748
30	23.562819794412956	44.87280182009143	12.937148053904593	18.62723033159102
31	20.072295354541193	48.12333691594938	12.422721134678804	19.381646594830624
32	19.37965625258362	53.469855501152864	12.46436521861613	14.686123027647385
33	23.579431497012955	50.23792245014192	13.542824509993048	12.63982154285207
34	20.384779087320908	50.95398634931425	13.954672251873218	14.706562311491625
35	22.069450696160477	51.32924241449949	12.619918120382023	13.98138876895801
36	21.585185117139297	50.500724178371414	16.565159212069435	11.348931492419858
37	18.33510933103066	51.896413403270905	16.656868058681415	13.111609207017027
38	14.966301974725715	56.73478230248915	16.674781138904454	11.624134583880677
39	17.505902130278617	56.248143623096546	14.312321443334955	11.933632803289882
40	20.54193957320938	54.39758464313164	12.849572995036393	12.21090278862259
41	22.082234817516238	52.58889940197871	12.387813593731341	12.941052186773716
42	20.397333553802014	51.49321599500271	12.110696711648401	15.998753739546876
43	21.906089528656338	49.55469919804518	13.196122200889837	15.34308907240865
44	20.51361547200201	48.175162365996385	14.734120896450149	16.577101265551462
45	19.92776588675871	47.400919231911615	16.259718228779125	16.411596652550546
46	21.80389310943514	45.36640669734856	13.952069496627134	18.877630696589165
47	24.581798473254395	43.48170569268503	13.70075051213037	18.235745321930203
48	24.726174837787106	43.19425434124265	14.563028014832643	17.516542806137604
49	23.238087801651677	45.04022022371446	15.089473539165343	16.632218435468513
50	22.99419432476874	46.02788928797803	14.16105543256261	16.816860954690625
51	21.888329551683064	43.63243584208318	16.715047293593855	17.764187312639898
52	24.360947035461773	40.64485557770449	16.187147288388342	18.80705009844539
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	6.0
2	12.0
3	26.0
4	40.0
5	136.5
6	233.0
7	1687.0
8	3141.0
9	4683.5
10	6226.0
11	5867.5
12	5509.0
13	7000.0
14	8986.5
15	9482.0
16	10989.0
17	12496.0
18	16027.5
19	19559.0
20	26951.5
21	34344.0
22	46194.0
23	58044.0
24	69693.5
25	81343.0
26	82668.5
27	83994.0
28	78473.0
29	72952.0
30	65328.5
31	57705.0
32	52214.5
33	46724.0
34	42739.5
35	38755.0
36	39699.0
37	40643.0
38	42018.0
39	46101.5
40	48810.0
41	51625.0
42	54440.0
43	53656.0
44	52872.0
45	55214.5
46	57557.0
47	58608.5
48	59660.0
49	56391.5
50	53123.0
51	54796.0
52	56469.0
53	60259.5
54	64050.0
55	69823.0
56	75596.0
57	64461.5
58	53327.0
59	42555.0
60	31783.0
61	28588.5
62	25394.0
63	19967.5
64	14916.0
65	15291.0
66	12785.5
67	10280.0
68	6466.5
69	2653.0
70	1753.5
71	854.0
72	3588.0
73	6322.0
74	3247.0
75	172.0
76	95.5
77	19.0
78	13.0
79	7.0
80	4.0
81	1.0
82	0.5
83	0.0
84	0.5
85	1.0
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1306308.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.77544065708314
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.01175602669838	31.998956702284424
2	5.641302465419842	4.149227680914083
3	1.900615413825808	2.096879080892655
4	0.9481850707290742	1.3947969520211698
5	0.6175245683312061	1.1354869058477575
6	0.4390019165324692	0.9686693357871352
7	0.34378623271129566	0.8850023139857499
8	0.2741095718551966	0.80644002346394
9	0.22371730316082275	0.7404572165718165
>10	2.231578328986449	16.931291275361986
>50	0.1706424932088063	4.257229388650073
>100	0.12687415636297072	11.060779831616308
>500	0.05154920113807311	13.575001414864312
>1k	0.019357251039602966	9.999781877738556
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCCACAATAGGGGCGGCACCGCCGCCTACGCCGGGCACGTCGCCAAGTACG	2624	0.20087146369768843	No Hit
CTTGACAATAGGGAGCTCGGGCTGCTCGTCCGCTCGGCCTCTCCCCCCAACC	2623	0.20079491207280367	No Hit
GTCAACAATAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTTTTC	2437	0.18655630984423277	No Hit
AATCACAATAGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTG	2426	0.18571424197050004	No Hit
CAGGACAATAGGGATGAACAGTTGCCCATACTGGGCAACCGCCCTAGAGAGC	2275	0.17415494661289682	No Hit
TATCACAATAGGGGCCCCTTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGA	2220	0.16994460724423338	No Hit
TTCGACAATAGGGGCCTTGGCCTGGCCTTAGCAGAGAGTATCTGTTGCAGTC	2174	0.16642323249953303	No Hit
GTTCACAATAGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAA	2171	0.16619357762487866	No Hit
TATGACAATAGGGCTTTTCTGCCTCCTCTGCCGCCATGGCGCCTGTGAAAAA	2070	0.1584618635115149	No Hit
GGTCACAATAGGGATTGATACCACATAGAAAGCATGCAGCCATAGACTTCTG	2033	0.15562945339077766	No Hit
GTGGACAATAGGGGAGTTTTCACAATAGGGGAGCCCACTTGGGTGGATTTTT	2006	0.15356255951888836	No Hit
GAGAACAATAGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTG	1966	0.15050049452349676	No Hit
GGTGACAATAGGGGATGAAAGCGTTGAAGGCTAAGTTCATTAAAATGACTTC	1959	0.14996463314930322	No Hit
TGTTACAATAGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGC	1918	0.14682601652902685	No Hit
CCGGACAATAGGGGGGTGGCTCACGCCTGTAATCCCAGCACTTTGGTTGGCC	1861	0.14246257391059383	No Hit
AGCCACAATAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCG	1814	0.13886464754100872	No Hit
TCTTACAATAGGGGCCTTTCCAGCCTCCGCCTCGGACCCTGCAGCCGCCGCG	1693	0.12960190092994914	No Hit
CCGCACAATAGGGGGGTGGTGGTGGCGTACGCCTTTAGTCCCAGCACTTGGG	1675	0.12822397168202293	No Hit
CTAGACAATAGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGC	1674	0.1281474200571381	No Hit
TTGTACAATAGGGGTCTGACCAGAGGCTCCTTGGGGCTCCCTGGACAGAGTC	1643	0.12577431968570965	No Hit
CTAGACAATAGGGCGAGCAGGAGATGGCCACTGCCGCATCCTCTTCCTCCCT	1636	0.12523845831151612	No Hit
GCGCACAATAGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTG	1615	0.12363087418893552	No Hit
GATGACAATAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCG	1569	0.1201094994442352	No Hit
TTTGACAATAGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGCT	1548	0.11850191532165463	No Hit
GACTACAATAGGGCTTTCGGCCGCCGCAGCCATCTTCCAGTAACTCGCCAAA	1543	0.11811915719723066	No Hit
TGCTACAATAGGGACACGCCTGTAACCCCAGCACTTTGGGAGGTGGGTAGAT	1525	0.11674122794930444	No Hit
ACGTACAATAGGGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTC	1521	0.1164350214497653	No Hit
TAACACAATAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCC	1480	0.11329640482948891	No Hit
CTTAACAATAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCG	1462	0.11191847558156269	No Hit
TGTTACAATAGGGCTTCCTGTCCCGGCCGCCATCGGAGAGCATCAGCCATGG	1438	0.11008123658432774	No Hit
ATTAACAATAGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAA	1425	0.10908606546082547	No Hit
ATTAACAATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	1398	0.10701917158893615	No Hit
CAATACAATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGAC	1393	0.1066364134645122	No Hit
TGTGACAATAGGGGGGTGGTGGTGGCGTACGCCTTTAGTCCCAGCACTTGGG	1390	0.10640675858985783	No Hit
AAGAACAATAGGGACAAAGTGAGACTTCATCTCTACAAAAAATTCAAAAAAT	1385	0.10602400046543388	No Hit
GATTACAATAGGGGGACCCTCCCAAACTGCTAGGGCTCTAGGAGGAGACCTA	1372	0.10502882934193161	No Hit
GTGGACAATAGGGGGTTCCCGGCAGCCCCCGCGGCGGCCGCTGAAGGTGAGC	1354	0.10365090009400539	No Hit
GTTTACAATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	1352	0.10349779684423581	No Hit
GTCTACAATAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCCTCCT	1340	0.10257917734561833	No Hit
GCCAACAATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACT	1334	0.10211986759630959	No Hit
CTGGACAATAGGGGAGTTACAACAATAGGGGATCTTTCCATCGGTGACCAGC	1321	0.10112469647280733	No Hit
GCATACAATAGAGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCG	1314	0.10058883509861381	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.23447762702211117	0.0	0.0	0.0	0.0
11	0.25116588124699535	0.0	0.0	0.0	0.0
12	0.36308435682855805	0.0	0.0	0.0	0.0
13	0.45165458682025983	0.0	0.0	0.0	0.0
14	0.7238721649105724	0.0	0.0	0.0	0.0
15	1.0957599586008813	0.0	0.0	0.0	0.0
16	1.2944114251769108	0.0	0.0	0.0	0.0
17	1.4469022619474121	0.0	0.0	0.0	0.0
18	1.6805378210957906	0.0	0.0	0.0	0.0
19	1.8671706825649081	0.0	0.0	0.0	0.0
20	2.030455298444165	0.0	0.0	0.0	0.0
21	2.215786782290241	7.65516248847898E-5	0.0	0.0	0.0
22	2.405405157129865	7.65516248847898E-5	0.0	0.0	0.0
23	2.568995979508661	7.65516248847898E-5	0.0	0.0	0.0
24	2.7502702272358435	7.65516248847898E-5	0.0	0.0	0.0
25	2.955887891676389	7.65516248847898E-5	0.0	0.0	0.0
26	3.2033027433040293	7.65516248847898E-5	0.0	0.0	0.0
27	3.47437204702107	7.65516248847898E-5	0.0	0.0	0.0
28	3.7219400018984805	7.65516248847898E-5	0.0	0.0	0.0
29	4.004951359097548	7.65516248847898E-5	0.0	0.0	0.0
30	4.339252304969425	7.65516248847898E-5	0.0	0.0	0.0
31	4.639870535891995	7.65516248847898E-5	0.0	0.0	0.0
32	4.908872945737146	7.65516248847898E-5	0.0	0.0	0.0
33	5.116787158924235	7.65516248847898E-5	0.0	0.0	0.0
34	5.303113813893813	7.65516248847898E-5	0.0	0.0	0.0
35	5.477651518631134	7.65516248847898E-5	0.0	0.0	0.0
36	5.65770094036016	7.65516248847898E-5	0.0	0.0	0.0
37	5.836678639340799	7.65516248847898E-5	0.0	0.0	0.0
38	6.025454946306691	7.65516248847898E-5	0.0	0.0	0.0
39	6.216068492269817	7.65516248847898E-5	0.0	0.0	0.0
40	6.418777194964741	7.65516248847898E-5	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACTG	20	6.3117716E-4	46.000004	24
ATCCTTT	55	1.8189894E-12	46.000004	18
CTTGTCC	80	0.0	46.000004	26
GCCCCGG	105	0.0	46.000004	23
ATTTAAG	55	1.8189894E-12	46.000004	15
ACAACCG	20	6.3117716E-4	46.000004	24
ATCCTAT	110	0.0	46.000004	40
ATCCTAG	40	5.6115823E-9	46.000004	14
CTATGTT	110	0.0	46.000004	39
GTGCACG	40	5.6115823E-9	46.000004	28
CTAGAGG	40	5.6115823E-9	46.000004	45
CATGGAA	105	0.0	46.000004	29
CTATGGC	20	6.3117716E-4	46.000004	37
TAATAAT	20	6.3117716E-4	46.000004	25
CTGTCGA	80	0.0	46.000004	37
TCGTTCT	40	5.6115823E-9	46.000004	16
CTGTCAT	55	1.8189894E-12	46.000004	42
GAAGCTA	20	6.3117716E-4	46.000004	13
ACCGGTA	40	5.6115823E-9	46.000004	46
GATGTTT	40	5.6115823E-9	46.000004	38
>>END_MODULE