FastQCFastQC Report
Fri 17 Jun 2016
SRR1528169_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528169_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1301246
Sequences flagged as poor quality0
Sequence length52
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCACAATAGGGGCGGCACCGCCGCCTACGCCGGGCACGTCGCCAAGTACG26280.20196027499796348No Hit
CTTGACAATAGGGAGCTCGGGCTGCTCGTCCGCTCGGCCTCTCCCCCCAACC25700.19750300865478165No Hit
GTCAACAATAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTTTTC23670.1819025764536452No Hit
AATCACAATAGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTG23390.1797507927017643No Hit
CAGGACAATAGGGATGAACAGTTGCCCATACTGGGCAACCGCCCTAGAGAGC22180.17045201291685047No Hit
TTCGACAATAGGGGCCTTGGCCTGGCCTTAGCAGAGAGTATCTGTTGCAGTC21850.16791598206641942No Hit
GTTCACAATAGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAA21770.16730118670873914No Hit
TATGACAATAGGGCTTTTCTGCCTCCTCTGCCGCCATGGCGCCTGTGAAAAA20840.16015419067570622No Hit
TATCACAATAGGGGCCCCTTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGA20740.15938569647860587No Hit
GGTCACAATAGGGATTGATACCACATAGAAAGCATGCAGCCATAGACTTCTG20180.1550821289748441No Hit
GTGGACAATAGGGGAGTTTTCACAATAGGGGAGCCCACTTGGGTGGATTTTT19830.152392399284993No Hit
TGTTACAATAGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGC19600.15062486263166228No Hit
GAGAACAATAGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTG18660.1434010171789193No Hit
CCGGACAATAGGGGGGTGGCTCACGCCTGTAATCCCAGCACTTTGGTTGGCC18560.14263252298181897No Hit
AGCCACAATAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCG18480.1420177276241387No Hit
GGTGACAATAGGGGATGAAAGCGTTGAAGGCTAAGTTCATTAAAATGACTTC18210.13994279329196785No Hit
CCGCACAATAGGGGGGTGGTGGTGGCGTACGCCTTTAGTCCCAGCACTTGGG17330.13318004435748507No Hit
ACGTACAATAGGGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTC16520.12695524136097247No Hit
GCGCACAATAGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTG16380.12587934948503204No Hit
CTAGACAATAGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGC16210.1245729093499615No Hit
TTGTACAATAGGGGTCTGACCAGAGGCTCCTTGGGGCTCCCTGGACAGAGTC16180.1243423610908314No Hit
TGCTACAATAGGGACACGCCTGTAACCCCAGCACTTTGGGAGGTGGGTAGAT15970.12272852327692074No Hit
TCTTACAATAGGGGCCTTTCCAGCCTCCGCCTCGGACCCTGCAGCCGCCGCG15870.12196002907982043No Hit
GACTACAATAGGGCTTTCGGCCGCCGCAGCCATCTTCCAGTAACTCGCCAAA15640.12019249242648969No Hit
CTAGACAATAGGGCGAGCAGGAGATGGCCACTGCCGCATCCTCTTCCTCCCT15070.11581207550301786No Hit
TTTGACAATAGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGCT14920.1146593342073674No Hit
AAGAACAATAGGGACAAAGTGAGACTTCATCTCTACAAAAAATTCAAAAAAT14690.11289179755403667No Hit
GATGACAATAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCG14620.11235385161606644No Hit
TAACACAATAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCC14340.11020206786418556No Hit
TGTTACAATAGGGCTTCCTGTCCCGGCCGCCATCGGAGAGCATCAGCCATGG14190.10904932656853508No Hit
ATTAACAATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT13940.10712809107578428No Hit
CTGGACAATAGGGGAGTTACAACAATAGGGGATCTTTCCATCGGTGACCAGC13940.10712809107578428No Hit
CTTAACAATAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCG13900.10682069339694417No Hit
ATTAACAATAGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAA13660.1049763073239034No Hit
GTCTACAATAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCCTCCT13640.10482260848448334No Hit
GCATACAATAGAGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCG13550.10413096370709306No Hit
GTGGACAATAGGGGGTTCCCGGCAGCCCCCGCGGCGGCCGCTGAAGGTGAGC13550.10413096370709306No Hit
TGTGACAATAGGGGGGTGGTGGTGGCGTACGCCTTTAGTCCCAGCACTTGGG13370.10274767415231248No Hit
GTGGACAATAGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGCT13190.10136438459753191No Hit
GTTTACAATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT13180.10128753517782187No Hit
CAATACAATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGAC13140.10098013749898174No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTTTG206.311762E-446.00000425
ATCCTTG750.046.00000424
ATTTAGT405.6115823E-946.00000431
ATTTAAG750.046.00000415
CTTCGCC750.046.00000430
CAGCGTA1600.046.00000440
AACGTAT206.311762E-446.00000426
TGTCAAG206.311762E-446.00000415
GAAATAC800.046.00000440
GATACTT405.6115823E-946.00000413
GTACCTT750.046.00000439
CTGTCCG750.046.00000438
TACCCTA206.311762E-446.00000432
CAAACTT206.311762E-446.00000424
AATCCAG405.6115823E-946.00000423
TCTTGAA206.311762E-446.00000445
TCGCACC206.311762E-446.00000443
CTGTATT750.046.00000442
CTGTAGA206.311762E-446.00000431
TTGGACT405.6115823E-946.00000424
Produced by FastQC (version 0.11.5)