##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528169_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1301246
Sequences flagged as poor quality	0
Sequence length	52
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.916247965411614	31.0	31.0	34.0	28.0	34.0
2	31.695315105675636	31.0	31.0	34.0	30.0	34.0
3	31.95587613717929	31.0	31.0	34.0	30.0	34.0
4	35.63164459295168	37.0	35.0	37.0	33.0	37.0
5	35.60394575660559	37.0	35.0	37.0	33.0	37.0
6	35.79726738833395	37.0	35.0	37.0	35.0	37.0
7	35.58802486232427	37.0	35.0	37.0	33.0	37.0
8	35.54240089883081	37.0	35.0	37.0	33.0	37.0
9	37.34701970265422	39.0	37.0	39.0	34.0	39.0
10	36.81706917831063	39.0	37.0	39.0	32.0	39.0
11	37.465775110932135	39.0	37.0	39.0	35.0	39.0
12	37.5464062905861	39.0	37.0	39.0	35.0	39.0
13	37.44824191582529	39.0	37.0	39.0	35.0	39.0
14	37.802563081846166	40.0	38.0	40.0	33.0	40.0
15	36.963491146178356	39.0	36.0	40.0	31.0	40.0
16	36.36021244253585	38.0	35.0	40.0	31.0	40.0
17	36.006234024926876	38.0	35.0	40.0	30.0	40.0
18	36.091181067991755	38.0	35.0	40.0	30.0	40.0
19	36.40145137814064	39.0	35.0	40.0	31.0	40.0
20	36.52739989210342	39.0	35.0	40.0	31.0	40.0
21	36.580924744437255	39.0	35.0	40.0	31.0	40.0
22	36.59064773301897	39.0	35.0	40.0	31.0	40.0
23	36.6820739506596	39.0	35.0	40.0	31.0	40.0
24	36.75793816080895	39.0	35.0	40.0	31.0	40.0
25	36.7911332676527	39.0	35.0	40.0	31.0	40.0
26	36.50788321347385	39.0	35.0	40.0	31.0	40.0
27	36.246293168240285	39.0	35.0	40.0	30.0	40.0
28	36.056801711590275	38.0	35.0	40.0	30.0	40.0
29	35.92690928540799	38.0	35.0	40.0	30.0	40.0
30	35.69360828006388	38.0	35.0	40.0	30.0	40.0
31	35.666551904866566	38.0	35.0	40.0	30.0	40.0
32	35.697708196605404	38.0	35.0	40.0	30.0	40.0
33	35.591658302888156	38.0	35.0	40.0	30.0	40.0
34	35.39435663971302	38.0	34.0	40.0	29.0	40.0
35	35.08970940160431	37.0	34.0	40.0	26.0	40.0
36	34.94531625841693	37.0	34.0	40.0	26.0	40.0
37	34.70536393579692	37.0	34.0	40.0	25.0	40.0
38	34.33933860315421	36.0	33.0	40.0	24.0	40.0
39	34.01087803535996	36.0	33.0	40.0	23.0	40.0
40	33.58174780172235	35.0	33.0	40.0	21.0	40.0
41	33.000565611729066	35.0	33.0	40.0	18.0	40.0
42	32.398075383132785	35.0	32.0	39.0	15.0	40.0
43	31.818206549722344	35.0	31.0	39.0	10.0	40.0
44	31.10057206708032	35.0	30.0	39.0	10.0	40.0
45	30.69772126100676	35.0	28.0	38.0	9.0	40.0
46	30.24545704655384	35.0	26.0	38.0	8.0	40.0
47	29.810152730536732	35.0	24.0	38.0	8.0	40.0
48	29.416059684333323	35.0	23.0	38.0	8.0	40.0
49	28.951652493072025	35.0	22.0	37.0	8.0	40.0
50	28.63414834704583	34.0	20.0	37.0	8.0	40.0
51	28.24298480072177	34.0	20.0	37.0	8.0	40.0
52	27.170375931991337	33.0	15.0	35.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	2.0
14	12.0
15	32.0
16	92.0
17	322.0
18	805.0
19	1652.0
20	2995.0
21	4750.0
22	7214.0
23	10186.0
24	13841.0
25	18884.0
26	25349.0
27	32431.0
28	41892.0
29	52891.0
30	63394.0
31	67315.0
32	64266.0
33	68221.0
34	88403.0
35	129146.0
36	127123.0
37	192497.0
38	240902.0
39	46627.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.154648698247684	21.788040078509365	35.885912425475276	8.171398797767678
2	20.188035160146505	23.675384977168036	36.59285023738786	19.543729625297598
3	21.47779897113997	23.188620752724695	35.70792916942685	19.625651106708496
4	20.345115374033814	24.89152704407929	32.63548936941977	22.127868212467128
5	0.0	100.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	0.0	100.0	0.0	0.0
8	0.0	100.0	0.0	0.0
9	0.0	0.0	100.0	0.0
10	0.0	100.0	0.0	0.0
11	99.3028220643906	0.6015004080704186	0.06570625385207716	0.02997127368691239
12	98.76479927700066	1.0473807412280232	0.11504358130591755	0.07277640046540008
13	97.06934737935794	1.9822539320005594	0.5912025858292744	0.35719610281222763
14	58.607211856943266	27.995475106167472	6.566782914222215	6.830530122667043
15	36.24864168650663	32.21404715172996	17.372349271390654	14.164961890372766
16	39.583983351341715	25.564497412480037	19.508071494552144	15.343447741626104
17	23.034691365045504	28.56054888929534	24.061553311210947	24.343206434448213
18	20.559832652703637	38.96619086629276	25.609608021849827	14.864368459153765
19	26.063788092336114	37.834736859901966	24.980288123844378	11.121186923917538
20	20.675183631688395	38.00949244032258	26.748209024273656	14.56711490371536
21	26.15362506397714	37.2573671696205	16.64558430919288	19.943423457209477
22	23.983781698464394	44.82073335864241	15.936341014688999	15.259143928204196
23	18.640979491963854	44.34249941978688	18.87391008310496	18.1426110051443
24	16.833173742705068	48.752349671007636	15.946024041572462	18.468452544714836
25	21.031534390883813	42.62914160735172	18.543227030092694	17.79609697167177
26	18.401132453048845	42.29868910259859	17.408699046913497	21.89147939743907
27	19.936660708274992	48.895981236445685	13.599119613047803	17.568238442231525
28	20.794069683979817	45.25739176143481	14.752552553475667	19.195986001109706
29	22.05632140271709	43.22480145952418	13.740215147635421	20.978661990123314
30	23.55926550398618	44.88159809905275	13.06271066347178	18.496425733489286
31	20.00444189645924	48.212866744643215	12.412948819823463	19.369742539074085
32	19.287974756502614	53.658109227617224	12.411949777367232	14.641966238512932
33	23.593386646337432	50.34651403347253	13.44872529867527	12.611374021514763
34	20.3411960536286	51.057294316370616	13.886766991022451	14.714742638978334
35	21.78926966922473	51.423328102449496	12.807416891195055	13.979985337130717
36	21.565330460189696	50.56768666339801	16.554056650318234	11.312926226094067
37	18.320210014094187	52.044501961965686	16.57572818667646	13.059559837263668
38	14.981563824211563	56.768435791541336	16.637899367221877	11.61210101702522
39	17.495000945247863	56.35268043091006	14.194395218121707	11.957923405720363
40	20.513723000877622	54.5228188981945	12.833238296217624	12.130219804710254
41	22.008521063657447	52.70732820696471	12.43100843345532	12.853142295922524
42	20.34227194550454	51.581714756471875	12.028778570693014	16.047234727330572
43	22.005447086869047	49.54558938125459	13.201193317789258	15.247770214087113
44	20.447709349346706	48.18435560993079	14.841621030919598	16.52631400980291
45	19.892472291941722	47.457513798313315	16.272480376500678	16.37753353324429
46	21.853823181781156	45.39710400646765	13.988438773298823	18.760634038452377
47	24.592736500246687	43.66706986995541	13.577217528430443	18.16297610136746
48	24.787703478051036	43.33254434595764	14.465750519117831	17.41400165687349
49	23.016631751413644	45.19452893611201	15.273207372011132	16.51563194046322
50	23.00802461640612	46.118105262187164	14.134990616685853	16.73887950472086
51	21.757530858884486	43.76605192254193	16.731194562749856	17.745222655823724
52	24.513120501427093	40.53668560748698	16.2351315585216	18.71506233256433
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	6.5
2	13.0
3	27.5
4	42.0
5	110.0
6	178.0
7	1632.0
8	3086.0
9	4648.0
10	6210.0
11	5791.5
12	5373.0
13	6835.5
14	8742.5
15	9187.0
16	10813.0
17	12439.0
18	16085.0
19	19731.0
20	26897.0
21	34063.0
22	45845.0
23	57627.0
24	69485.0
25	81343.0
26	82824.5
27	84306.0
28	78681.0
29	73056.0
30	65578.5
31	58101.0
32	52574.0
33	47047.0
34	42947.0
35	38847.0
36	39555.5
37	40264.0
38	41965.5
39	46145.5
40	48624.0
41	51683.5
42	54743.0
43	53772.0
44	52801.0
45	55171.0
46	57541.0
47	58079.5
48	58618.0
49	55637.0
50	52656.0
51	53997.0
52	55338.0
53	59688.0
54	64038.0
55	69333.5
56	74629.0
57	63699.5
58	52770.0
59	42074.5
60	31379.0
61	28550.5
62	25722.0
63	20001.0
64	14719.0
65	15158.0
66	12648.0
67	10138.0
68	6355.5
69	2573.0
70	1706.5
71	840.0
72	3580.5
73	6321.0
74	3246.0
75	171.0
76	97.0
77	23.0
78	13.5
79	4.0
80	2.5
81	1.0
82	0.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1301246.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.51446129635176
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.10482406068553	32.67690550950117
2	5.773377571900349	4.33170298940562
3	1.8224867693514206	2.051088281158411
4	0.9179047206258857	1.3773880446263347
5	0.5903281479390179	1.1072921229002652
6	0.4082524797296805	0.9189223109975239
7	0.3385968268049837	0.8891594287970157
8	0.26505759804858076	0.7954794402637957
9	0.23190005091870722	0.7829644936330661
>10	2.1910131834711932	16.897059091110464
>50	0.15989660130376263	4.048689587015202
>100	0.13001512618074873	11.547955809247938
>500	0.048008754932827406	12.96937229222391
>1k	0.01833810810738901	9.606020599119319
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCCACAATAGGGGCGGCACCGCCGCCTACGCCGGGCACGTCGCCAAGTACG	2628	0.20196027499796348	No Hit
CTTGACAATAGGGAGCTCGGGCTGCTCGTCCGCTCGGCCTCTCCCCCCAACC	2570	0.19750300865478165	No Hit
GTCAACAATAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTTTTC	2367	0.1819025764536452	No Hit
AATCACAATAGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTG	2339	0.1797507927017643	No Hit
CAGGACAATAGGGATGAACAGTTGCCCATACTGGGCAACCGCCCTAGAGAGC	2218	0.17045201291685047	No Hit
TTCGACAATAGGGGCCTTGGCCTGGCCTTAGCAGAGAGTATCTGTTGCAGTC	2185	0.16791598206641942	No Hit
GTTCACAATAGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAA	2177	0.16730118670873914	No Hit
TATGACAATAGGGCTTTTCTGCCTCCTCTGCCGCCATGGCGCCTGTGAAAAA	2084	0.16015419067570622	No Hit
TATCACAATAGGGGCCCCTTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGA	2074	0.15938569647860587	No Hit
GGTCACAATAGGGATTGATACCACATAGAAAGCATGCAGCCATAGACTTCTG	2018	0.1550821289748441	No Hit
GTGGACAATAGGGGAGTTTTCACAATAGGGGAGCCCACTTGGGTGGATTTTT	1983	0.152392399284993	No Hit
TGTTACAATAGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGC	1960	0.15062486263166228	No Hit
GAGAACAATAGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTG	1866	0.1434010171789193	No Hit
CCGGACAATAGGGGGGTGGCTCACGCCTGTAATCCCAGCACTTTGGTTGGCC	1856	0.14263252298181897	No Hit
AGCCACAATAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCG	1848	0.1420177276241387	No Hit
GGTGACAATAGGGGATGAAAGCGTTGAAGGCTAAGTTCATTAAAATGACTTC	1821	0.13994279329196785	No Hit
CCGCACAATAGGGGGGTGGTGGTGGCGTACGCCTTTAGTCCCAGCACTTGGG	1733	0.13318004435748507	No Hit
ACGTACAATAGGGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTC	1652	0.12695524136097247	No Hit
GCGCACAATAGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTG	1638	0.12587934948503204	No Hit
CTAGACAATAGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGC	1621	0.1245729093499615	No Hit
TTGTACAATAGGGGTCTGACCAGAGGCTCCTTGGGGCTCCCTGGACAGAGTC	1618	0.1243423610908314	No Hit
TGCTACAATAGGGACACGCCTGTAACCCCAGCACTTTGGGAGGTGGGTAGAT	1597	0.12272852327692074	No Hit
TCTTACAATAGGGGCCTTTCCAGCCTCCGCCTCGGACCCTGCAGCCGCCGCG	1587	0.12196002907982043	No Hit
GACTACAATAGGGCTTTCGGCCGCCGCAGCCATCTTCCAGTAACTCGCCAAA	1564	0.12019249242648969	No Hit
CTAGACAATAGGGCGAGCAGGAGATGGCCACTGCCGCATCCTCTTCCTCCCT	1507	0.11581207550301786	No Hit
TTTGACAATAGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGCT	1492	0.1146593342073674	No Hit
AAGAACAATAGGGACAAAGTGAGACTTCATCTCTACAAAAAATTCAAAAAAT	1469	0.11289179755403667	No Hit
GATGACAATAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCG	1462	0.11235385161606644	No Hit
TAACACAATAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCC	1434	0.11020206786418556	No Hit
TGTTACAATAGGGCTTCCTGTCCCGGCCGCCATCGGAGAGCATCAGCCATGG	1419	0.10904932656853508	No Hit
ATTAACAATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	1394	0.10712809107578428	No Hit
CTGGACAATAGGGGAGTTACAACAATAGGGGATCTTTCCATCGGTGACCAGC	1394	0.10712809107578428	No Hit
CTTAACAATAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCG	1390	0.10682069339694417	No Hit
ATTAACAATAGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAA	1366	0.1049763073239034	No Hit
GTCTACAATAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCCTCCT	1364	0.10482260848448334	No Hit
GCATACAATAGAGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCG	1355	0.10413096370709306	No Hit
GTGGACAATAGGGGGTTCCCGGCAGCCCCCGCGGCGGCCGCTGAAGGTGAGC	1355	0.10413096370709306	No Hit
TGTGACAATAGGGGGGTGGTGGTGGCGTACGCCTTTAGTCCCAGCACTTGGG	1337	0.10274767415231248	No Hit
GTGGACAATAGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGCT	1319	0.10136438459753191	No Hit
GTTTACAATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	1318	0.10128753517782187	No Hit
CAATACAATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGAC	1314	0.10098013749898174	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.23300744056081632	0.0	0.0	0.0	0.0
11	0.24906896928021297	0.0	0.0	0.0	0.0
12	0.357964597009328	0.0	0.0	0.0	0.0
13	0.45387267280744764	0.0	0.0	0.0	0.0
14	0.7371396338586247	0.0	0.0	0.0	0.0
15	1.1203876899525531	0.0	0.0	0.0	0.0
16	1.311281648512272	0.0	0.0	0.0	0.0
17	1.4566039011839422	0.0	0.0	0.0	0.0
18	1.6892270946462082	0.0	0.0	0.0	0.0
19	1.8830413311549083	0.0	0.0	0.0	0.0
20	2.0459621009401756	0.0	0.0	0.0	0.0
21	2.2271730326164305	0.0	0.0	0.0	0.0
22	2.4169910993002093	0.0	0.0	0.0	0.0
23	2.5751472050634545	0.0	0.0	0.0	0.0
24	2.7531304611118883	0.0	0.0	0.0	0.0
25	2.960162797810714	0.0	0.0	0.0	0.0
26	3.1917100993970395	0.0	0.0	0.0	0.0
27	3.466677323119533	0.0	0.0	0.0	0.0
28	3.710213134180624	0.0	0.0	0.0	0.0
29	3.99055981728282	0.0	0.0	0.0	0.0
30	4.32969630646319	0.0	0.0	0.0	0.0
31	4.64293454120128	0.0	0.0	0.0	0.0
32	4.923665471402026	0.0	0.0	0.0	0.0
33	5.148757421732708	0.0	0.0	0.0	0.0
34	5.335424662208376	0.0	0.0	0.0	0.0
35	5.513254219417389	0.0	0.0	0.0	0.0
36	5.689700487071622	0.0	0.0	0.0	0.0
37	5.860613596506733	0.0	0.0	0.0	0.0
38	6.043745763675738	0.0	0.0	0.0	0.0
39	6.233025884421547	0.0	0.0	0.0	0.0
40	6.428607657583577	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTTTG	20	6.311762E-4	46.000004	25
ATCCTTG	75	0.0	46.000004	24
ATTTAGT	40	5.6115823E-9	46.000004	31
ATTTAAG	75	0.0	46.000004	15
CTTCGCC	75	0.0	46.000004	30
CAGCGTA	160	0.0	46.000004	40
AACGTAT	20	6.311762E-4	46.000004	26
TGTCAAG	20	6.311762E-4	46.000004	15
GAAATAC	80	0.0	46.000004	40
GATACTT	40	5.6115823E-9	46.000004	13
GTACCTT	75	0.0	46.000004	39
CTGTCCG	75	0.0	46.000004	38
TACCCTA	20	6.311762E-4	46.000004	32
CAAACTT	20	6.311762E-4	46.000004	24
AATCCAG	40	5.6115823E-9	46.000004	23
TCTTGAA	20	6.311762E-4	46.000004	45
TCGCACC	20	6.311762E-4	46.000004	43
CTGTATT	75	0.0	46.000004	42
CTGTAGA	20	6.311762E-4	46.000004	31
TTGGACT	40	5.6115823E-9	46.000004	24
>>END_MODULE