FastQCFastQC Report
Fri 17 Jun 2016
SRR1528168_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528168_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1244604
Sequences flagged as poor quality0
Sequence length59
%GC39

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGACAATAGGGAGCTCGGGCTGCTCGTCCGCTCGGCCTCTCCCCCCAACCCTTTTGC22630.18182490173581314No Hit
GCCCACAATAGGGGCGGCACCGCCGCCTACGCCGGGCACGTCGCCAAGTACGCCATCGA21720.17451333918258338No Hit
CAGGACAATAGGGATGAACAGTTGCCCATACTGGGCAACCGCCCTAGAGAGCCAGAAAG21400.1719422402627663No Hit
AATCACAATAGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCTTCT21340.1714601592153006No Hit
GTCAACAATAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTTTTCCCTCCAT18320.14719541315952706No Hit
TATGACAATAGGGCTTTTCTGCCTCCTCTGCCGCCATGGCGCCTGTGAAAAAGCTTGTG18260.14671333211206133No Hit
TTCGACAATAGGGGCCTTGGCCTGGCCTTAGCAGAGAGTATCTGTTGCAGTCATGTCGG17890.14374049898602287No Hit
GGTGACAATAGGGGATGAAAGCGTTGAAGGCTAAGTTCATTAAAATGACTTCTTCGAGT17680.14205321531989293No Hit
TATCACAATAGGGGCCCCTTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGG16770.1347416527666631No Hit
GTTCACAATAGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAA16530.1328133285768003No Hit
CCGGACAATAGGGGGGTGGCTCACGCCTGTAATCCCAGCACTTTGGTTGGCCGAGGAGG16390.13168847279938037No Hit
TGTTACAATAGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCTG15960.12823355862587618No Hit
GGTCACAATAGGGATTGATACCACATAGAAAGCATGCAGCCATAGACTTCTGGGGACAG15690.12606419391228055No Hit
GTGGACAATAGGGGAGTTTTCACAATAGGGGAGCCCACTTGGGTGGATTTTTTTTTTTT15650.1257428065473034No Hit
GAGAACAATAGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTTTTCT15600.125341072341082No Hit
GCGCACAATAGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCTTCT14910.11979714029522644No Hit
CTAGACAATAGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCTTCTG14780.11875263135905074No Hit
AGCCACAATAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGAAGC14730.11835089715282934No Hit
CCGCACAATAGGGGGGTGGTGGTGGCGTACGCCTTTAGTCCCAGCACTTGGGAGGCAGA14670.11786881610536364No Hit
CTGGACAATAGGGGAGTTACAACAATAGGGGATCTTTCCATCGGTGACCAGCAAATGGT13990.11240523090075237No Hit
AAGAACAATAGGGACAAAGTGAGACTTCATCTCTACAAAAAATTCAAAAAATTAGCCAA13750.11047690671088957No Hit
TCTTACAATAGGGGCCTTTCCAGCCTCCGCCTCGGACCCTGCAGCCGCCGCGATGTTGA13750.11047690671088957No Hit
GATTACAATAGGGGGACCCTCCCAAACTGCTAGGGCTCTAGGAGGAGACCTAGGTCTGT13680.10991447882217958No Hit
CTAGACAATAGGGCGAGCAGGAGATGGCCACTGCCGCATCCTCTTCCTCCCTGGAGAAG13060.10493297466503401No Hit
GACTACAATAGGGCTTTCGGCCGCCGCAGCCATCTTCCAGTAACTCGCCAAAATGACGA12950.1040491594113469No Hit
TTTGACAATAGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCTTCTGA12820.10300465047517121No Hit
TGCTACAATAGGGACACGCCTGTAACCCCAGCACTTTGGGAGGTGGGTAGATTAGTTAA12740.10236187574521696No Hit
TTGTACAATAGGGGTCTGACCAGAGGCTCCTTGGGGCTCCCTGGACAGAGTCCCCTGCC12630.10147806049152983No Hit
GATGACAATAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGAAGC12560.10091563260281985No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATTC503.6379788E-1253.00000444
CTGTCTA503.6379788E-1253.00000449
TAATACT503.6379788E-1253.00000422
ATCTCTC750.053.00000423
TACCCTG503.6379788E-1253.00000428
AATCCAC750.053.00000444
ATGATTC1350.053.00000424
TCCGCGG550.053.00000428
CATATAG503.6379788E-1253.00000442
TAAAGTA550.053.00000419
TCTCTAT550.053.00000438
ACTTGCG550.053.00000435
CTTTTAC750.053.00000417
GTTTAAT503.6379788E-1253.00000441
CTATATT251.4788033E-553.00000415
CTATACA251.4788033E-553.0000041
TGTGGGC1000.053.00000434
GCTATAT251.4788033E-553.00000414
ACCGATA550.053.00000415
GTTAGTC251.4788033E-553.00000442
Produced by FastQC (version 0.11.5)