##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1528168_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1244604 Sequences flagged as poor quality 0 Sequence length 59 %GC 39 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.43169956066347 31.0 31.0 33.0 27.0 34.0 2 31.29562977461104 31.0 31.0 34.0 28.0 34.0 3 31.594073295602456 31.0 31.0 34.0 30.0 34.0 4 35.33373105019749 37.0 35.0 37.0 33.0 37.0 5 35.068795375878594 37.0 35.0 37.0 32.0 37.0 6 35.49137396312401 37.0 35.0 37.0 33.0 37.0 7 35.149312552426316 37.0 35.0 37.0 32.0 37.0 8 35.147007401551015 37.0 35.0 37.0 32.0 37.0 9 37.02644053851667 39.0 37.0 39.0 33.0 39.0 10 36.54063461149089 38.0 35.0 39.0 32.0 39.0 11 36.69069760341442 38.0 35.0 39.0 33.0 39.0 12 37.050485937695846 39.0 37.0 39.0 34.0 39.0 13 36.968682408219806 39.0 37.0 39.0 33.0 39.0 14 37.0177767386253 40.0 36.0 40.0 31.0 40.0 15 36.14916792811207 38.0 35.0 40.0 30.0 40.0 16 35.371971325819295 37.0 34.0 40.0 28.0 40.0 17 35.20482820238405 38.0 33.0 40.0 26.0 40.0 18 35.37649967379183 38.0 34.0 40.0 27.0 40.0 19 35.76145183528255 38.0 34.0 40.0 27.0 40.0 20 35.8074375464003 38.0 34.0 40.0 29.0 40.0 21 35.808660425324035 38.0 34.0 40.0 29.0 40.0 22 35.80158427901566 38.0 34.0 40.0 29.0 40.0 23 35.78825072071117 38.0 34.0 40.0 29.0 40.0 24 35.86328101147031 38.0 34.0 40.0 30.0 40.0 25 35.9180703259832 38.0 34.0 40.0 30.0 40.0 26 35.59925405992589 38.0 34.0 40.0 27.0 40.0 27 35.33426294628653 38.0 34.0 40.0 27.0 40.0 28 35.0893231903481 37.0 34.0 40.0 26.0 40.0 29 34.90835237553471 37.0 34.0 40.0 26.0 40.0 30 34.68794652757021 36.0 33.0 40.0 26.0 40.0 31 34.66064306397858 36.0 33.0 40.0 25.0 40.0 32 34.60642019469647 36.0 33.0 40.0 25.0 40.0 33 34.27850625580506 36.0 33.0 40.0 24.0 40.0 34 33.99253015416952 36.0 33.0 40.0 23.0 40.0 35 33.86259645638292 35.0 33.0 40.0 23.0 40.0 36 33.53635774913145 35.0 33.0 39.0 22.0 40.0 37 33.43540355004483 35.0 33.0 39.0 21.0 40.0 38 33.12413667319083 35.0 32.0 39.0 21.0 40.0 39 32.76182384115751 35.0 31.0 39.0 18.0 40.0 40 32.42654691773448 35.0 31.0 39.0 18.0 40.0 41 31.817725959421633 35.0 30.0 38.0 15.0 40.0 42 31.30709205498295 35.0 30.0 38.0 12.0 40.0 43 30.756309637442914 35.0 28.0 38.0 10.0 40.0 44 30.12819177826843 35.0 26.0 38.0 9.0 40.0 45 29.782429592063018 34.0 24.0 38.0 9.0 40.0 46 29.286716899511813 34.0 23.0 37.0 8.0 40.0 47 28.698654351102842 33.0 22.0 37.0 8.0 40.0 48 28.303487695684733 33.0 20.0 37.0 8.0 40.0 49 27.890137746624628 33.0 20.0 36.0 8.0 40.0 50 27.417639666914134 33.0 18.0 36.0 8.0 39.0 51 27.022009410222047 33.0 17.0 35.0 8.0 39.0 52 26.296133549305644 33.0 13.0 35.0 8.0 39.0 53 26.036610038212956 32.0 12.0 35.0 8.0 39.0 54 25.702988259719557 32.0 10.0 35.0 8.0 39.0 55 25.34904997894913 31.0 10.0 35.0 8.0 38.0 56 24.849962719065662 31.0 10.0 35.0 8.0 38.0 57 24.291760270736717 30.0 10.0 35.0 8.0 38.0 58 23.841717526217174 29.0 9.0 35.0 8.0 37.0 59 23.02857615755694 27.0 9.0 35.0 8.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 2.0 14 18.0 15 140.0 16 464.0 17 1262.0 18 2831.0 19 5151.0 20 8563.0 21 12464.0 22 17569.0 23 23722.0 24 30991.0 25 39651.0 26 49818.0 27 59056.0 28 63020.0 29 58558.0 30 56443.0 31 62699.0 32 73442.0 33 86974.0 34 105885.0 35 117912.0 36 116875.0 37 132228.0 38 110744.0 39 8122.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.44653881877288 22.22851605811969 35.18605114558526 8.138893977522168 2 19.923124142297468 24.277922937737625 36.09557738847055 19.703375531494355 3 21.449232044891385 23.48514065518028 35.49136914231354 19.57425815761479 4 20.49655954825792 25.021773993977202 32.3935966781402 22.088069779624682 5 0.0 100.0 0.0 0.0 6 0.0 0.0 0.0 100.0 7 0.0 100.0 0.0 0.0 8 0.0 100.0 0.0 0.0 9 0.0 0.0 100.0 0.0 10 0.0 100.0 0.0 0.0 11 99.22392986042146 0.6690481470411472 0.061224293028143896 0.04579769950924149 12 98.76788118951892 1.0375991078286748 0.1059774836012097 0.08854221905120022 13 97.05657381785693 2.0078675626946403 0.561785113980029 0.373773505468406 14 57.763031454181416 28.596404960935367 6.788263576205765 6.852300008677458 15 36.293793045820195 32.61029210897603 17.457842012399123 13.638072832804651 16 39.75232282718037 25.559455055583946 19.529183579676747 15.159038537558933 17 23.10751050133215 28.275017595958236 24.08219803246655 24.535273870243067 18 20.511664754411846 39.1244122628563 25.407358485108517 14.95656449762334 19 26.217495685374626 37.84464777551735 25.034950875941263 10.902905663166758 20 20.56750580907662 37.98549578821858 27.080340413496984 14.366657989207813 21 26.19090088092277 37.192472465137506 16.928758062805517 19.687868591134208 22 23.99686968706512 44.875157078074636 15.919762430459809 15.208210804400435 23 18.30413529122516 44.432606676501116 19.280831493390668 17.98242653888305 24 16.4118064862398 49.3767495524681 15.995690195435659 18.215753765856448 25 20.62913183631099 42.90248143184499 18.675980472503703 17.79240625934032 26 18.175741038916797 42.69060681148381 17.38070904480461 21.752943104794777 27 19.78115127381882 49.44657095750938 13.611719068876527 17.160558699795274 28 20.517128339616455 45.87684114786711 14.568730294937184 19.037300217579244 29 21.947462807447188 43.511028407429194 13.507910949988913 21.03359783513471 30 23.541222750368792 45.48105260789777 12.811705570607195 18.16601907112624 31 19.84976747624144 48.788128593512475 12.151977657150386 19.2101262730957 32 19.075223926646547 54.64292256814216 12.198096744024605 14.083756761186692 33 23.177251559532188 51.42928995889455 13.274905110380491 12.118553371192766 34 19.935015474801624 52.12461152302258 13.758432400988587 14.181940601187204 35 21.619888735694246 52.60380008420349 12.436003740948928 13.340307439153337 36 20.933164283579355 51.70536170540991 16.489341188040534 10.872132822970197 37 17.68048310948703 53.378022246433396 16.430125566043497 12.511369078036067 38 14.504774209306737 58.09349801221914 16.143689077007625 11.258038701466491 39 16.851946482575983 57.84458349804436 13.862481560399933 11.440988458979724 40 19.78878422373703 56.211935683960526 12.3619239533217 11.637356138980753 41 21.317141837885785 54.44623350077615 11.79917467724674 12.437449984091325 42 19.624153546027493 53.28538233847875 11.493535293153485 15.596928822340278 43 21.068789751599706 51.515984200597146 12.623051187365618 14.792174860437537 44 19.60776279041366 50.019042201374894 14.219060841842063 16.15413416636938 45 18.932768977120435 49.3386651497183 15.747498802832066 15.981067070329196 46 20.89740993922565 47.22265073870886 13.359831721575699 18.520107600489794 47 23.711236666441696 45.17332420593217 13.194718962818698 17.92072016480744 48 23.812714726933226 45.04509064730629 13.914224926161253 17.227969699599228 49 22.43388258434008 46.560271379490985 14.608662675035594 16.39718336113334 50 21.911708463093483 47.65411327619066 13.903056715228299 16.53112154548756 51 21.00676199015912 45.10816291768305 16.36657121461927 17.518503877538556 52 22.077865730786662 43.03159880572455 16.311935362573156 18.578600100915633 53 21.58943728286266 43.484112215612356 16.209171752621717 18.717278748903265 54 19.64552580579847 44.6437581752911 16.64457128532449 19.066144733585944 55 21.122863175757107 41.36536601200061 19.791917750545554 17.719853061696732 56 20.13483806897616 40.72604619621984 19.985071556896813 19.15404417790719 57 19.968600454441734 39.13252729382197 19.42079569083821 21.478076560898085 58 23.01133533236274 37.781414811458106 19.361419375158682 19.845830481020467 59 24.5797860202924 35.40563906270589 19.476797439185475 20.53777747781624 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 4.0 2 8.0 3 18.0 4 85.5 5 143.0 6 1212.0 7 2281.0 8 3449.0 9 4254.0 10 3891.0 11 4661.5 12 5432.0 13 5245.0 14 5302.5 15 5547.0 16 6093.0 17 6639.0 18 8369.5 19 14660.0 20 19220.0 21 27725.5 22 36231.0 23 43813.0 24 51395.0 25 56678.5 26 65326.5 27 68691.0 28 67505.0 29 66319.0 30 64164.0 31 58019.0 32 54029.0 33 51732.5 34 49436.0 35 47211.5 36 43373.5 37 41760.0 38 42914.5 39 44069.0 40 46215.0 41 48361.0 42 47077.0 43 44383.5 44 42974.0 45 41218.0 46 39462.0 47 42415.0 48 41912.5 49 38457.0 50 41647.5 51 44838.0 52 49012.0 53 56112.0 54 59038.0 55 53358.5 56 47679.0 57 43135.0 58 31447.5 59 24304.0 60 22366.5 61 20429.0 62 19582.0 63 18735.0 64 15445.5 65 9250.0 66 6344.0 67 5845.5 68 5347.0 69 4148.0 70 3232.5 71 3516.0 72 2629.5 73 1743.0 74 1569.0 75 732.5 76 70.0 77 41.0 78 12.0 79 6.5 80 1.0 81 2.5 82 2.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 53 0.0 54 0.0 55 0.0 56 0.0 57 0.0 58 0.0 59 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 59 1244604.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.005339356319666 #Duplication Level Percentage of deduplicated Percentage of total 1 91.86799463057945 47.77626236747837 2 3.959773902614693 4.1185877115955085 3 1.2313953027551106 1.9211739180467258 4 0.6222551893943552 1.2944236916273764 5 0.37364821665819137 0.9715851153596449 6 0.25561811387765027 0.797610405469774 7 0.1968073678649756 0.7164523767549469 8 0.15065196981499746 0.6267765451941575 9 0.12122223019210418 0.5673782896803249 >10 1.0447368012111724 10.80330270209917 >50 0.05733058366997899 2.035434830327652 >100 0.07899144798588531 10.836238974202228 >500 0.03062292642619468 11.256346891488933 >1k 0.008951316955349214 6.278426180675211 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTGACAATAGGGAGCTCGGGCTGCTCGTCCGCTCGGCCTCTCCCCCCAACCCTTTTGC 2263 0.18182490173581314 No Hit GCCCACAATAGGGGCGGCACCGCCGCCTACGCCGGGCACGTCGCCAAGTACGCCATCGA 2172 0.17451333918258338 No Hit CAGGACAATAGGGATGAACAGTTGCCCATACTGGGCAACCGCCCTAGAGAGCCAGAAAG 2140 0.1719422402627663 No Hit AATCACAATAGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCTTCT 2134 0.1714601592153006 No Hit GTCAACAATAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTTTTCCCTCCAT 1832 0.14719541315952706 No Hit TATGACAATAGGGCTTTTCTGCCTCCTCTGCCGCCATGGCGCCTGTGAAAAAGCTTGTG 1826 0.14671333211206133 No Hit TTCGACAATAGGGGCCTTGGCCTGGCCTTAGCAGAGAGTATCTGTTGCAGTCATGTCGG 1789 0.14374049898602287 No Hit GGTGACAATAGGGGATGAAAGCGTTGAAGGCTAAGTTCATTAAAATGACTTCTTCGAGT 1768 0.14205321531989293 No Hit TATCACAATAGGGGCCCCTTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGG 1677 0.1347416527666631 No Hit GTTCACAATAGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAA 1653 0.1328133285768003 No Hit CCGGACAATAGGGGGGTGGCTCACGCCTGTAATCCCAGCACTTTGGTTGGCCGAGGAGG 1639 0.13168847279938037 No Hit TGTTACAATAGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCTG 1596 0.12823355862587618 No Hit GGTCACAATAGGGATTGATACCACATAGAAAGCATGCAGCCATAGACTTCTGGGGACAG 1569 0.12606419391228055 No Hit GTGGACAATAGGGGAGTTTTCACAATAGGGGAGCCCACTTGGGTGGATTTTTTTTTTTT 1565 0.1257428065473034 No Hit GAGAACAATAGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTTTTCT 1560 0.125341072341082 No Hit GCGCACAATAGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCTTCT 1491 0.11979714029522644 No Hit CTAGACAATAGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCTTCTG 1478 0.11875263135905074 No Hit AGCCACAATAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGAAGC 1473 0.11835089715282934 No Hit CCGCACAATAGGGGGGTGGTGGTGGCGTACGCCTTTAGTCCCAGCACTTGGGAGGCAGA 1467 0.11786881610536364 No Hit CTGGACAATAGGGGAGTTACAACAATAGGGGATCTTTCCATCGGTGACCAGCAAATGGT 1399 0.11240523090075237 No Hit AAGAACAATAGGGACAAAGTGAGACTTCATCTCTACAAAAAATTCAAAAAATTAGCCAA 1375 0.11047690671088957 No Hit TCTTACAATAGGGGCCTTTCCAGCCTCCGCCTCGGACCCTGCAGCCGCCGCGATGTTGA 1375 0.11047690671088957 No Hit GATTACAATAGGGGGACCCTCCCAAACTGCTAGGGCTCTAGGAGGAGACCTAGGTCTGT 1368 0.10991447882217958 No Hit CTAGACAATAGGGCGAGCAGGAGATGGCCACTGCCGCATCCTCTTCCTCCCTGGAGAAG 1306 0.10493297466503401 No Hit GACTACAATAGGGCTTTCGGCCGCCGCAGCCATCTTCCAGTAACTCGCCAAAATGACGA 1295 0.1040491594113469 No Hit TTTGACAATAGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCTTCTGA 1282 0.10300465047517121 No Hit TGCTACAATAGGGACACGCCTGTAACCCCAGCACTTTGGGAGGTGGGTAGATTAGTTAA 1274 0.10236187574521696 No Hit TTGTACAATAGGGGTCTGACCAGAGGCTCCTTGGGGCTCCCTGGACAGAGTCCCCTGCC 1263 0.10147806049152983 No Hit GATGACAATAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGAAGC 1256 0.10091563260281985 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.20689311620402956 0.0 0.0 0.0 0.0 11 0.22023069185058058 0.0 0.0 0.0 0.0 12 0.3135133745351935 0.0 0.0 0.0 0.0 13 0.39723478311173677 0.0 8.034684124428332E-5 0.0 0.0 14 0.6416498741768466 0.0 8.034684124428332E-5 0.0 0.0 15 0.9615909960115828 0.0 8.034684124428332E-5 0.0 0.0 16 1.1259806331973865 0.0 8.034684124428332E-5 0.0 0.0 17 1.2528482955221099 0.0 8.034684124428332E-5 0.0 0.0 18 1.4507425655067796 0.0 8.034684124428332E-5 0.0 0.0 19 1.6120790227253006 0.0 8.034684124428332E-5 0.0 0.0 20 1.7510790580779108 0.0 8.034684124428332E-5 0.0 0.0 21 1.901166957522232 0.0 8.034684124428332E-5 0.0 0.0 22 2.0629854957882188 0.0 8.034684124428332E-5 0.0 0.0 23 2.2033514274419814 0.0 8.034684124428332E-5 0.0 0.0 24 2.354644529504967 0.0 8.034684124428332E-5 0.0 0.0 25 2.5271491976564433 0.0 8.034684124428332E-5 0.0 0.0 26 2.737657921716466 0.0 8.034684124428332E-5 0.0 0.0 27 2.9594152035506878 0.0 8.034684124428332E-5 0.0 0.0 28 3.1687187249920457 0.0 8.034684124428332E-5 0.0 0.0 29 3.41265173500969 0.0 8.034684124428332E-5 0.0 0.0 30 3.697883021426896 0.0 8.034684124428332E-5 0.0 0.0 31 3.9564391565509993 0.0 8.034684124428332E-5 0.0 0.0 32 4.196033437141452 0.0 8.034684124428332E-5 0.0 0.0 33 4.387339266144091 0.0 8.034684124428332E-5 0.0 0.0 34 4.551327169123673 0.0 8.034684124428332E-5 0.0 0.0 35 4.713788482119614 0.0 8.034684124428332E-5 0.0 0.0 36 4.868777538879836 0.0 8.034684124428332E-5 0.0 0.0 37 5.032524401335686 0.0 8.034684124428332E-5 0.0 0.0 38 5.1952267548553595 0.0 8.034684124428332E-5 0.0 0.0 39 5.3640354683095985 0.0 8.034684124428332E-5 0.0 0.0 40 5.5331655691288155 0.0 8.034684124428332E-5 0.0 0.0 41 5.70189393574181 0.0 8.034684124428332E-5 0.0 0.0 42 5.864355248737751 0.0 8.034684124428332E-5 0.0 0.0 43 6.033565696398211 0.0 8.034684124428332E-5 0.0 0.0 44 6.199481923567657 0.0 8.034684124428332E-5 0.0 0.0 45 6.357765200818895 0.0 8.034684124428332E-5 0.0 0.0 46 6.522395878528432 0.0 8.034684124428332E-5 0.0 0.0 47 6.67168030956031 0.0 8.034684124428332E-5 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCATTC 50 3.6379788E-12 53.000004 44 CTGTCTA 50 3.6379788E-12 53.000004 49 TAATACT 50 3.6379788E-12 53.000004 22 ATCTCTC 75 0.0 53.000004 23 TACCCTG 50 3.6379788E-12 53.000004 28 AATCCAC 75 0.0 53.000004 44 ATGATTC 135 0.0 53.000004 24 TCCGCGG 55 0.0 53.000004 28 CATATAG 50 3.6379788E-12 53.000004 42 TAAAGTA 55 0.0 53.000004 19 TCTCTAT 55 0.0 53.000004 38 ACTTGCG 55 0.0 53.000004 35 CTTTTAC 75 0.0 53.000004 17 GTTTAAT 50 3.6379788E-12 53.000004 41 CTATATT 25 1.4788033E-5 53.000004 15 CTATACA 25 1.4788033E-5 53.000004 1 TGTGGGC 100 0.0 53.000004 34 GCTATAT 25 1.4788033E-5 53.000004 14 ACCGATA 55 0.0 53.000004 15 GTTAGTC 25 1.4788033E-5 53.000004 42 >>END_MODULE