FastQCFastQC Report
Fri 17 Jun 2016
SRR1528167_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528167_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences539118
Sequences flagged as poor quality0
Sequence length52
%GC36

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTGTAATGGGGGCAGTGGTGTGATCTAGGCTCACTGCAACCTCTGCCTCC21920.4065900229634329No Hit
TACGGTAATGGGGCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCC20230.37524252575502953No Hit
GGGAGTAATGGGGGATCACCTACGGTCAGGAGTTTGAGACCAGCCTGACCAA18250.33851587222092383No Hit
TGCAGTAATGGGGGGCGCGGTGGCTCATGCCTGTAATCCCAGCACTTTGGGA17260.32015254545387095No Hit
TGGTGTAATGGGGTACCATGCAAGTAACTACAAGAAAAGATGGCTATAAAAA15140.28082905783149514No Hit
AAGTGTAATGGGGGTGAAACCCCGTCTCTACTAAAAATACAAAAAATTAGGC14520.2693287925834419No Hit
GATCGTAATGGGGGAGGAGCCTGGTGAGCTACAGTCCATGGGGTCGCAATAG13610.2524493710096862No Hit
ATCAGTAATGGGCACACCACCTTATCAAATAAAATATTCTTATAAAACGGCA12830.23798129537503848No Hit
AGAGGTAATGGGGGAGATTATTAGCAAAAGCATGTTAAGAATTAATTACCGA12230.2268520064253095No Hit
TGCGGTAATGGGGTGATCAAGACCTTTAGGAGAAGTGAGATGGGACATAGAG11570.21460978858060756No Hit
AATCGTAATGGAGAACGGTACCCAAACTTTCGCGGAGGTACTCACCATCCAG9630.17862508764315047No Hit
ACCGGTAATGGGGTGGGCGGACTCGGCTTCGTGATGACACTGCTCTACACCA9560.1773266705990154No Hit
CTAGGTAATGGGGAAAGAAAATTTTCCCTCCCCTATACTCTTTTTATAACAC9180.17027812093085373No Hit
TTAAGTAATGGGGGCAACGTGTGTACTCACAATAAGTTTTTTTATATTCACT9170.17009263278169157No Hit
GCAGGTAATGGGGAATGCTAAGAGTTCAGCTTGCCAGTTCATTTTTCTGTCC8760.16248761866604342No Hit
GTTCGTAATGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT8540.15840687938447612No Hit
AATAGTAATGGGGAAGAGTGGTATCAACGCAGATTACAGTAATGGGGAACTA8540.15840687938447612No Hit
CTGGGTAATGGGGTGGGATGCACCGAACGATATCTACGGGGCGTTGGTGCCG8460.15692297419117893No Hit
CACTGTAATGGGGAGGATGTAGGCCAAAAGAGGCTCTGTCCAGCCTGACCAA8420.15618102159453032No Hit
TGGGGTAATGGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATG8120.15061637711966583No Hit
CACAGTAATGGGGGTTCTAGGGTACATGTGCACAACGTGCAGGTTTGTTACA7960.14764856673307142No Hit
GTTCGTAATGGGGGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGC7530.13967257631909896No Hit
CGGTGTAATGGGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTT7440.13800318297663963No Hit
TAATGTAATGGGGGATATATTCCCAGTAGTGAGATTGCTGGATCATATGGTA7110.13188207405428867No Hit
TTTAGTAATGGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGC6840.12687389402691063No Hit
TGTTGTAATGGGGGCTTCTACCTCTTCCCCTTGCGCAAGCGCACTGCTTCTG6610.12260766659618118No Hit
GCTTGTAATGGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTC6560.12168022585037043No Hit
CAAGGTAATGGGGAAGCAGTGATATCAACGCAGAGTTATTGTAATAGGGGCT6460.11982534435874892No Hit
ACTTGTAATGGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAA6350.11778497471796527No Hit
AGCGGTAATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6190.11481716433137086No Hit
CGGGGTAATGGGGGACAACAATTTCCATCTCCCTTCTTCTCTGCATAATGAA5960.11055093690064141No Hit
CGCGGTAATGGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAA5940.11017996060231712No Hit
GACGGTAATGAGGTCTTACCTATAAAAGAATAATGATAAAAATTACAGGGCC5870.10888154355818208No Hit
ACGGGTAATGGGGGAGTGGGTGTAATGGGGGCACCACTACACCCAGCTAGTT5870.10888154355818208No Hit
GATGGTAATGGGCGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTG5750.10665568576823627No Hit
GGTGGTAATGGGGGGGAATTCCTGGAGATTGTCTCGTACGGTTAAGAGCCTC5690.10554275687326338No Hit
TATTGTAATGGGGCCTAAAGAACTGAGAATCCTGGGAATTTAACCATGTCAA5640.10461531612745263No Hit
TATTGTAATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5590.10368787538164187No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA351.0182157E-746.00000417
TGTGACC351.0182157E-746.00000429
AAACTTT750.046.00000424
CGTCAGC351.0182157E-746.00000424
GAGGTAC700.046.00000435
GTGTCTA351.0182157E-746.00000440
CAAGACC1400.046.00000418
AACGTCA351.0182157E-746.00000422
CCTACGC351.0182157E-746.00000413
CTTTATG351.0182157E-746.00000435
GGCACAC700.046.00000411
GGCATAT351.0182157E-746.00000446
ATAAGTT850.046.00000432
GAAACGT351.0182157E-746.00000420
AACGATA850.046.00000426
TTACCGA1400.046.00000446
TGCGCAA700.046.00000432
CTCCCCT700.046.00000428
TAAGTTT850.046.00000433
ATTAGCA1400.046.00000420
Produced by FastQC (version 0.11.5)