##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528167_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	539118
Sequences flagged as poor quality	0
Sequence length	52
%GC	36
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.31146057078413	31.0	31.0	33.0	27.0	33.0
2	31.265509591592192	31.0	31.0	34.0	28.0	34.0
3	31.654810264172223	31.0	31.0	34.0	30.0	34.0
4	35.40027044172148	37.0	35.0	37.0	33.0	37.0
5	35.45969713495005	37.0	35.0	37.0	33.0	37.0
6	32.61633074762853	35.0	32.0	35.0	28.0	36.0
7	34.33273235173005	35.0	35.0	36.0	32.0	37.0
8	35.13324170218765	36.0	35.0	37.0	33.0	37.0
9	37.31143645732474	39.0	37.0	39.0	34.0	39.0
10	37.149755712107556	39.0	37.0	39.0	33.0	39.0
11	37.626775585307854	39.0	37.0	39.0	35.0	39.0
12	37.670958862438276	39.0	37.0	39.0	35.0	39.0
13	37.39672576319099	39.0	37.0	39.0	35.0	39.0
14	37.469680107137954	40.0	37.0	40.0	32.0	40.0
15	36.63099915046428	38.0	35.0	40.0	31.0	40.0
16	36.98514796389659	39.0	36.0	40.0	32.0	40.0
17	36.777098891151844	39.0	36.0	40.0	31.0	40.0
18	36.9964349177731	39.0	36.0	40.0	32.0	40.0
19	37.11456675533	39.0	36.0	40.0	32.0	40.0
20	37.21251748225806	39.0	36.0	40.0	32.0	40.0
21	37.226145296577	39.0	36.0	40.0	32.0	40.0
22	37.28000549044921	39.0	36.0	40.0	32.0	40.0
23	37.19883587637586	39.0	36.0	40.0	32.0	40.0
24	37.11795933357818	39.0	36.0	40.0	32.0	40.0
25	37.12615234512667	39.0	36.0	40.0	32.0	40.0
26	36.85198602161308	39.0	36.0	40.0	31.0	40.0
27	36.37926947347334	39.0	35.0	40.0	30.0	40.0
28	35.74585897706996	37.0	35.0	40.0	30.0	40.0
29	35.32331882816007	36.0	34.0	40.0	30.0	40.0
30	35.038765168293395	35.0	34.0	40.0	29.0	40.0
31	34.89228888666303	35.0	33.0	40.0	28.0	40.0
32	34.72060105579855	35.0	33.0	40.0	28.0	40.0
33	34.39662745447193	35.0	33.0	40.0	25.0	40.0
34	34.06128157472019	35.0	33.0	40.0	23.0	40.0
35	33.82424626890588	35.0	33.0	40.0	22.0	40.0
36	33.4091219361995	35.0	33.0	39.0	21.0	40.0
37	33.05713034994194	35.0	33.0	39.0	19.0	40.0
38	32.440506531037734	35.0	33.0	39.0	15.0	40.0
39	31.576068689971397	35.0	31.0	39.0	11.0	40.0
40	30.681303536517053	35.0	27.0	39.0	9.0	40.0
41	29.661743811187904	35.0	23.0	38.0	8.0	40.0
42	28.70059059426693	35.0	18.0	38.0	8.0	40.0
43	27.997926242492365	35.0	15.0	38.0	8.0	40.0
44	27.453223598544287	34.0	12.0	38.0	8.0	40.0
45	27.086316910212606	34.0	10.0	37.0	8.0	40.0
46	26.715173672554062	33.0	10.0	37.0	8.0	40.0
47	26.160851613190435	33.0	10.0	36.0	8.0	40.0
48	25.798739793514592	33.0	10.0	36.0	8.0	40.0
49	25.47354011552202	33.0	10.0	35.0	8.0	40.0
50	25.113713509843855	33.0	9.0	35.0	8.0	39.0
51	24.7583571685605	32.0	9.0	35.0	8.0	39.0
52	23.943734024833155	30.0	9.0	35.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	3.0
14	3.0
15	13.0
16	44.0
17	124.0
18	312.0
19	836.0
20	1485.0
21	2746.0
22	4220.0
23	6384.0
24	9056.0
25	12449.0
26	16333.0
27	21451.0
28	27901.0
29	34346.0
30	37337.0
31	31944.0
32	25150.0
33	25682.0
34	34577.0
35	45885.0
36	55918.0
37	68964.0
38	67859.0
39	8096.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.2861859555793	22.674627818028707	38.20258273698893	4.836603489403062
2	28.749179214939957	20.50348903208574	33.985880642085775	16.761451110888526
3	21.822124284479465	20.57842624434725	37.17980108250884	20.41964838866445
4	25.580114186504627	21.302757466825444	33.29938900203666	19.81773934463327
5	100.0	0.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	0.0	100.0	0.0	0.0
8	0.0	100.0	0.0	0.0
9	0.0	0.0	100.0	0.0
10	100.0	0.0	0.0	0.0
11	98.6739452216398	1.021112261137636	0.13633378963418027	0.16860872758839437
12	97.70180183188096	1.466469307275958	0.3654116538494356	0.46631720699364515
13	84.585563828327	11.360036207286717	2.328432736432469	1.7259672279538059
14	28.96174863387978	51.984352219736685	12.305469303566195	6.748429842817343
15	20.563401704265114	58.90250371903739	14.212843941400585	6.321250635296911
16	28.187335611127807	48.806383760141564	13.877295879566255	9.128984749164376
17	15.108751701853768	51.91479416380087	14.382380109734788	18.594074024610567
18	13.709614592723671	58.61555355228354	15.953835709436524	11.72099614555626
19	18.885104930645984	54.43483615831784	20.102834629895497	6.577224281140678
20	14.737404427231143	52.9188415152156	21.658338248769287	10.685415808783977
21	19.575677309976665	50.85584232023416	12.830957230142568	16.73752313964661
22	19.744286037565058	55.3196146298213	14.4367281374393	10.49937119517434
23	15.596029069702736	53.20579168196944	18.604090384665323	12.5940888636625
24	15.677643855334084	58.159623681642984	12.613750607473689	13.54898185554925
25	15.329297111207566	54.68468869523926	17.810201106251323	12.175813087301853
26	13.96911251340152	56.47297994131155	13.256652532469701	16.301255012817233
27	13.345686844067533	61.4498124714812	11.490990840595195	13.713509843856075
28	14.544125775804185	59.231745183800214	12.971371759058314	13.252757281337296
29	18.667156355380456	54.43891689759941	13.23680530050935	13.657121446510784
30	21.67614511108885	57.004403488661104	10.503822910754232	10.815628489495806
31	16.473388015239706	60.544259327271575	10.379360362666429	12.602992294822284
32	15.354338011344455	63.43268078602458	11.88385474052063	9.329126462110336
33	18.122748637589545	61.98624419885813	11.46038529598344	8.430621867568881
34	14.390727076447085	64.27461149507158	11.974187469162596	9.360473959318739
35	17.543840124054473	60.778159883365056	13.789003520565071	7.888996472015402
36	13.59813621507722	60.834548280710344	15.323917954881864	10.243397549330572
37	14.292418357391146	61.139119821634594	15.516640141861338	9.051821679112921
38	15.478615071283095	58.23029466647376	16.161953412796457	10.12913684944669
39	17.138177541836853	55.947121038436855	15.053290745254285	11.861410674472008
40	21.21780389450918	53.57769542103955	14.094687990384294	11.109812694066976
41	22.140792924740037	51.41471811365972	15.035298394785556	11.409190566814686
42	21.637563576063126	50.16452798830683	16.0794111864193	12.118497249210748
43	21.9959266802444	51.62914241409117	14.328774034626928	12.04615687103751
44	22.089412707422124	48.34563119762279	16.66091653404264	12.904039560912453
45	24.406159690457375	43.86609239535686	17.686295022611006	14.041452891574757
46	28.31365304070723	45.38041764511666	14.18835950571118	12.117569808464937
47	28.377089987720684	45.88049369525781	13.893804324841685	11.84861199217982
48	26.449126165329297	46.85764526504402	13.952975044424413	12.740253525202275
49	23.482985172077356	51.03335447898234	10.88147678244837	14.602183566491936
50	22.284731728489866	49.75682503644842	13.380744104259179	14.577699130802532
51	21.507350895351294	51.419540805537935	12.554023423443475	14.519084875667293
52	23.36019201733201	48.646863951862116	13.17466677054003	14.818277260265841
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	2.0
4	4.0
5	8.0
6	12.0
7	451.0
8	890.0
9	2050.0
10	3210.0
11	6089.0
12	8968.0
13	9490.5
14	9281.5
15	8550.0
16	7587.0
17	6624.0
18	7911.0
19	9198.0
20	11307.0
21	13416.0
22	17384.5
23	21353.0
24	26018.0
25	30683.0
26	34061.5
27	37440.0
28	38042.5
29	38645.0
30	35889.0
31	33133.0
32	30628.5
33	28124.0
34	26585.5
35	25047.0
36	23268.5
37	21490.0
38	21783.0
39	21877.5
40	21679.0
41	23558.5
42	25438.0
43	26590.0
44	27742.0
45	28706.0
46	29670.0
47	29336.0
48	29002.0
49	26796.0
50	24590.0
51	21513.0
52	18436.0
53	15849.5
54	13263.0
55	12515.0
56	11767.0
57	8305.5
58	4844.0
59	5244.5
60	5645.0
61	5528.0
62	5411.0
63	3609.0
64	1192.0
65	577.0
66	360.0
67	143.0
68	104.5
69	66.0
70	92.5
71	119.0
72	70.5
73	22.0
74	16.0
75	10.0
76	7.5
77	5.0
78	5.0
79	5.0
80	3.0
81	1.0
82	0.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	539118.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.547065862160956
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.82376301560467	47.19975191168116
2	4.4443804422858575	4.670783036345899
3	1.4185773332372478	2.236262296805589
4	0.7869690401921283	1.6541165594582943
5	0.4730778043337126	1.2429425271125045
6	0.3437152225770411	1.0836735863149856
7	0.2739096531508663	1.0075204009080163
8	0.2178578992232963	0.9158234703262859
9	0.19566408407669442	0.9253416161553717
>10	1.8175303380070256	19.407661792272
>50	0.11466414226526833	4.093832310583257
>100	0.07467325308954778	8.358938331420024
>500	0.01167875522253591	4.273288480671445
>1k	0.0035390167341017905	2.930063679945136
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTTGTAATGGGGGCAGTGGTGTGATCTAGGCTCACTGCAACCTCTGCCTCC	2192	0.4065900229634329	No Hit
TACGGTAATGGGGCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCC	2023	0.37524252575502953	No Hit
GGGAGTAATGGGGGATCACCTACGGTCAGGAGTTTGAGACCAGCCTGACCAA	1825	0.33851587222092383	No Hit
TGCAGTAATGGGGGGCGCGGTGGCTCATGCCTGTAATCCCAGCACTTTGGGA	1726	0.32015254545387095	No Hit
TGGTGTAATGGGGTACCATGCAAGTAACTACAAGAAAAGATGGCTATAAAAA	1514	0.28082905783149514	No Hit
AAGTGTAATGGGGGTGAAACCCCGTCTCTACTAAAAATACAAAAAATTAGGC	1452	0.2693287925834419	No Hit
GATCGTAATGGGGGAGGAGCCTGGTGAGCTACAGTCCATGGGGTCGCAATAG	1361	0.2524493710096862	No Hit
ATCAGTAATGGGCACACCACCTTATCAAATAAAATATTCTTATAAAACGGCA	1283	0.23798129537503848	No Hit
AGAGGTAATGGGGGAGATTATTAGCAAAAGCATGTTAAGAATTAATTACCGA	1223	0.2268520064253095	No Hit
TGCGGTAATGGGGTGATCAAGACCTTTAGGAGAAGTGAGATGGGACATAGAG	1157	0.21460978858060756	No Hit
AATCGTAATGGAGAACGGTACCCAAACTTTCGCGGAGGTACTCACCATCCAG	963	0.17862508764315047	No Hit
ACCGGTAATGGGGTGGGCGGACTCGGCTTCGTGATGACACTGCTCTACACCA	956	0.1773266705990154	No Hit
CTAGGTAATGGGGAAAGAAAATTTTCCCTCCCCTATACTCTTTTTATAACAC	918	0.17027812093085373	No Hit
TTAAGTAATGGGGGCAACGTGTGTACTCACAATAAGTTTTTTTATATTCACT	917	0.17009263278169157	No Hit
GCAGGTAATGGGGAATGCTAAGAGTTCAGCTTGCCAGTTCATTTTTCTGTCC	876	0.16248761866604342	No Hit
GTTCGTAATGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT	854	0.15840687938447612	No Hit
AATAGTAATGGGGAAGAGTGGTATCAACGCAGATTACAGTAATGGGGAACTA	854	0.15840687938447612	No Hit
CTGGGTAATGGGGTGGGATGCACCGAACGATATCTACGGGGCGTTGGTGCCG	846	0.15692297419117893	No Hit
CACTGTAATGGGGAGGATGTAGGCCAAAAGAGGCTCTGTCCAGCCTGACCAA	842	0.15618102159453032	No Hit
TGGGGTAATGGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATG	812	0.15061637711966583	No Hit
CACAGTAATGGGGGTTCTAGGGTACATGTGCACAACGTGCAGGTTTGTTACA	796	0.14764856673307142	No Hit
GTTCGTAATGGGGGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGC	753	0.13967257631909896	No Hit
CGGTGTAATGGGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTT	744	0.13800318297663963	No Hit
TAATGTAATGGGGGATATATTCCCAGTAGTGAGATTGCTGGATCATATGGTA	711	0.13188207405428867	No Hit
TTTAGTAATGGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGC	684	0.12687389402691063	No Hit
TGTTGTAATGGGGGCTTCTACCTCTTCCCCTTGCGCAAGCGCACTGCTTCTG	661	0.12260766659618118	No Hit
GCTTGTAATGGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTC	656	0.12168022585037043	No Hit
CAAGGTAATGGGGAAGCAGTGATATCAACGCAGAGTTATTGTAATAGGGGCT	646	0.11982534435874892	No Hit
ACTTGTAATGGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAA	635	0.11778497471796527	No Hit
AGCGGTAATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	619	0.11481716433137086	No Hit
CGGGGTAATGGGGGACAACAATTTCCATCTCCCTTCTTCTCTGCATAATGAA	596	0.11055093690064141	No Hit
CGCGGTAATGGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAA	594	0.11017996060231712	No Hit
GACGGTAATGAGGTCTTACCTATAAAAGAATAATGATAAAAATTACAGGGCC	587	0.10888154355818208	No Hit
ACGGGTAATGGGGGAGTGGGTGTAATGGGGGCACCACTACACCCAGCTAGTT	587	0.10888154355818208	No Hit
GATGGTAATGGGCGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTG	575	0.10665568576823627	No Hit
GGTGGTAATGGGGGGGAATTCCTGGAGATTGTCTCGTACGGTTAAGAGCCTC	569	0.10554275687326338	No Hit
TATTGTAATGGGGCCTAAAGAACTGAGAATCCTGGGAATTTAACCATGTCAA	564	0.10461531612745263	No Hit
TATTGTAATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	559	0.10368787538164187	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.16712482239509718	0.0	0.0	0.0	0.0
12	0.3021601949851424	0.0	0.0	0.0	0.0
13	0.626764456018905	0.0	0.0	0.0	0.0
14	1.3891207490753417	0.0	0.0	0.0	0.0
15	2.0242321718065432	0.0	0.0	0.0	0.0
16	2.3751757500213313	0.0	0.0	0.0	0.0
17	2.5853338230220473	0.0	0.0	0.0	0.0
18	2.8490979711306244	0.0	0.0	0.0	0.0
19	3.086893778356501	0.0	0.0	0.0	0.0
20	3.3436093767969166	0.0	0.0	0.0	0.0
21	3.5955022833591164	0.0	0.0	0.0	0.0
22	3.889500999781124	0.0	0.0	0.0	0.0
23	4.167362247226024	0.0	0.0	0.0	0.0
24	4.457836688813952	0.0	0.0	0.0	0.0
25	4.790416940261687	0.0	0.0	0.0	0.0
26	5.149707485188771	0.0	0.0	0.0	0.0
27	5.491005679647127	0.0	0.0	0.0	0.0
28	5.834900708193754	0.0	0.0	0.0	0.0
29	6.219232153257728	0.0	0.0	0.0	0.0
30	6.634169142933458	0.0	0.0	0.0	0.0
31	6.985854673744894	0.0	0.0	0.0	0.0
32	7.246280035168553	0.0	0.0	0.0	0.0
33	7.4492040703519455	0.0	0.0	0.0	0.0
34	7.641555281033095	0.0	0.0	0.0	0.0
35	7.8021880182075165	0.0	0.0	0.0	0.0
36	7.96467563687356	0.0	0.0	0.0	0.0
37	8.121784099213901	0.0	0.0	0.0	0.0
38	8.278150608957594	0.0	0.0	0.0	0.0
39	8.44175115651861	0.0	0.0	0.0	0.0
40	8.618150386371815	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGAAA	35	1.0182157E-7	46.000004	17
TGTGACC	35	1.0182157E-7	46.000004	29
AAACTTT	75	0.0	46.000004	24
CGTCAGC	35	1.0182157E-7	46.000004	24
GAGGTAC	70	0.0	46.000004	35
GTGTCTA	35	1.0182157E-7	46.000004	40
CAAGACC	140	0.0	46.000004	18
AACGTCA	35	1.0182157E-7	46.000004	22
CCTACGC	35	1.0182157E-7	46.000004	13
CTTTATG	35	1.0182157E-7	46.000004	35
GGCACAC	70	0.0	46.000004	11
GGCATAT	35	1.0182157E-7	46.000004	46
ATAAGTT	85	0.0	46.000004	32
GAAACGT	35	1.0182157E-7	46.000004	20
AACGATA	85	0.0	46.000004	26
TTACCGA	140	0.0	46.000004	46
TGCGCAA	70	0.0	46.000004	32
CTCCCCT	70	0.0	46.000004	28
TAAGTTT	85	0.0	46.000004	33
ATTAGCA	140	0.0	46.000004	20
>>END_MODULE