FastQCFastQC Report
Fri 17 Jun 2016
SRR1528166_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528166_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences539294
Sequences flagged as poor quality0
Sequence length52
%GC36

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGGTAATGGGGCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCC22220.41202015969026173No Hit
ACTTGTAATGGGGGCAGTGGTGTGATCTAGGCTCACTGCAACCTCTGCCTCC20600.3819808861214848No Hit
GGGAGTAATGGGGGATCACCTACGGTCAGGAGTTTGAGACCAGCCTGACCAA18620.3452662184263129No Hit
TGCAGTAATGGGGGGCGCGGTGGCTCATGCCTGTAATCCCAGCACTTTGGGA17220.3193063523792217No Hit
TGGTGTAATGGGGTACCATGCAAGTAACTACAAGAAAAGATGGCTATAAAAA15610.2894525064250668No Hit
AAGTGTAATGGGGGTGAAACCCCGTCTCTACTAAAAATACAAAAAATTAGGC14670.27202231065059135No Hit
GATCGTAATGGGGGAGGAGCCTGGTGAGCTACAGTCCATGGGGTCGCAATAG13030.24161218185257022No Hit
ATCAGTAATGGGCACACCACCTTATCAAATAAAATATTCTTATAAAACGGCA12770.23679106387239612No Hit
TGCGGTAATGGGGTGATCAAGACCTTTAGGAGAAGTGAGATGGGACATAGAG12300.2280759659851584No Hit
AGAGGTAATGGGGGAGATTATTAGCAAAAGCATGTTAAGAATTAATTACCGA11770.21824830241018814No Hit
AATCGTAATGGAGAACGGTACCCAAACTTTCGCGGAGGTACTCACCATCCAG9520.17652708912022014No Hit
AATAGTAATGGGGAAGAGTGGTATCAACGCAGATTACAGTAATGGGGAACTA9270.17189139875466813No Hit
ACCGGTAATGGGGTGGGCGGACTCGGCTTCGTGATGACACTGCTCTACACCA9180.17022255022306942No Hit
TTAAGTAATGGGGGCAACGTGTGTACTCACAATAAGTTTTTTTATATTCACT8880.16465972178440702No Hit
GCAGGTAATGGGGAATGCTAAGAGTTCAGCTTGCCAGTTCATTTTTCTGTCC8800.16317630086743037No Hit
CTAGGTAATGGGGAAAGAAAATTTTCCCTCCCCTATACTCTTTTTATAACAC8530.15816975527263424No Hit
GTTCGTAATGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT8490.1574280448141459No Hit
CACTGTAATGGGGAGGATGTAGGCCAAAAGAGGCTCTGTCCAGCCTGACCAA8110.15038179545850688No Hit
CTGGGTAATGGGGTGGGATGCACCGAACGATATCTACGGGGCGTTGGTGCCG7940.1472295260099315No Hit
CACAGTAATGGGGGTTCTAGGGTACATGTGCACAACGTGCAGGTTTGTTACA7840.1453752498637107No Hit
TGGGGTAATGGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATG7600.1409249871127808No Hit
CGGTGTAATGGGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTT7450.13814357289344958No Hit
GTTCGTAATGGGGGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGC7390.1370310072057171No Hit
TTTAGTAATGGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGC7180.13313702729865343No Hit
TAATGTAATGGGGGATATATTCCCAGTAGTGAGATTGCTGGATCATATGGTA6760.12534906748452607No Hit
CGCGGTAATGGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAA6520.12089880473359614No Hit
ACTTGTAATGGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAA6510.12071337711897408No Hit
CAAGGTAATGGGGAAGCAGTGATATCAACGCAGAGTTATTGTAATAGGGGCT6430.11922995620199743No Hit
CGGGGTAATGGGGGACAACAATTTCCATCTCCCTTCTTCTCTGCATAATGAA6300.11681939721191038No Hit
GCTTGTAATGGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTC6210.11515054868031166No Hit
AGCGGTAATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6090.11292541730484672No Hit
TGTTGTAATGGGGGCTTCTACCTCTTCCCCTTGCGCAAGCGCACTGCTTCTG5980.11088571354400382No Hit
GACGGTAATGAGGTCTTACCTATAAAAGAATAATGATAAAAATTACAGGGCC5920.10977314785627136No Hit
TATTGTAATGGGGCCTAAAGAACTGAGAATCCTGGGAATTTAACCATGTCAA5810.10773344409542847No Hit
ACGGGTAATGGGGGAGTGGGTGTAATGGGGGCACCACTACACCCAGCTAGTT5730.10625002317845184No Hit
GGTGGTAATGGGGGGGAATTCCTGGAGATTGTCTCGTACGGTTAAGAGCCTC5550.10291232611525439No Hit
GATGGTAATGGGCGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTG5500.101985188042144No Hit
GTCAGTAATGGGGAAATATGAAAGAAAATATCTCCTTCAGTGGTCGAAATAA5460.10124347758365566No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACAAG1450.046.00000428
ATTAGCA1450.046.00000420
GGACATA1050.046.00000443
CCATGCA1450.046.00000416
TAGCAAA1450.046.00000422
TAACTAC1450.046.00000425
TACAAGA1450.046.00000429
AACTACA1450.046.00000426
ACGGGTA1650.046.0000041
TAATTAC1450.046.00000443
TTTAGGA1050.046.00000425
TTAGGAG1050.046.00000426
ATGCAAG1450.046.00000418
TATTAGC1450.046.00000419
GTAACTA1450.046.00000424
ACTACAA1450.046.00000427
CATGCAA1450.046.00000417
TGAGACA206.308459E-446.037
ACAAATG301.8595529E-646.023
CGCGAAA206.308459E-446.017
Produced by FastQC (version 0.11.5)