SRR1528165_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1528165_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	510994
Sequences flagged as poor quality	0
Sequence length	59
%GC	35

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
ACTTGTAATGGGGGCAGTGGTGTGATCTAGGCTCACTGCAACCTCTGCCTCCCAGCTTC	1889	0.3696716595498186	No Hit
GGGAGTAATGGGGGATCACCTACGGTCAGGAGTTTGAGACCAGCCTGACCAACATGGTG	1725	0.3375773492448052	No Hit
TACGGTAATGGGGCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGG	1601	0.31331091950199025	No Hit
TGGTGTAATGGGGTACCATGCAAGTAACTACAAGAAAAGATGGCTATAAAAACAAAATA	1441	0.2819993972531967	No Hit
TGCAGTAATGGGGGGCGCGGTGGCTCATGCCTGTAATCCCAGCACTTTGGGACGCCGAT	1343	0.26282108987581065	No Hit
AAGTGTAATGGGGGTGAAACCCCGTCTCTACTAAAAATACAAAAAATTAGGCAGGCGGG	1262	0.24696963173735897	No Hit
GATCGTAATGGGGGAGGAGCCTGGTGAGCTACAGTCCATGGGGTCGCAATAGTCGGATA	1202	0.23522781089406136	No Hit
ATCAGTAATGGGCACACCACCTTATCAAATAAAATATTCTTATAAAACGGCAGCATTCT	1159	0.22681283928969814	No Hit
AGAGGTAATGGGGGAGATTATTAGCAAAAGCATGTTAAGAATTAATTACCGAATTCATA	1000	0.19569701405495957	No Hit
TGCGGTAATGGGGTGATCAAGACCTTTAGGAGAAGTGAGATGGGACATAGAGCCAAGAA	958	0.18747773946465124	No Hit
AATCGTAATGGAGAACGGTACCCAAACTTTCGCGGAGGTACTCACCATCCAGATGATAT	909	0.17788858577595823	No Hit
AATAGTAATGGGGAAGAGTGGTATCAACGCAGATTACAGTAATGGGGAACTATCTAATA	880	0.1722133723683644	No Hit
GCAGGTAATGGGGAATGCTAAGAGTTCAGCTTGCCAGTTCATTTTTCTGTCCTATGTTA	855	0.16732094701699043	No Hit
CTAGGTAATGGGGAAAGAAAATTTTCCCTCCCCTATACTCTTTTTATAACACAGAATAT	835	0.16340700673589123	No Hit
TTAAGTAATGGGGGCAACGTGTGTACTCACAATAAGTTTTTTTATATTCACTCAATGAG	832	0.16281991569372634	No Hit
GTTCGTAATGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGA	789	0.1544049440893631	No Hit
CACTGTAATGGGGAGGATGTAGGCCAAAAGAGGCTCTGTCCAGCCTGACCAACATGGAG	766	0.14990391276609902	No Hit
ACCGGTAATGGGGTGGGCGGACTCGGCTTCGTGATGACACTGCTCTACACCAAAAAAAA	722	0.1412932441476808	No Hit
CACAGTAATGGGGGTTCTAGGGTACATGTGCACAACGTGCAGGTTTGTTACATATGTAT	672	0.13150839344493281	No Hit
TAATGTAATGGGGGATATATTCCCAGTAGTGAGATTGCTGGATCATATGGTAGTTCCAT	656	0.12837724122005345	No Hit
TGGGGTAATGGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATGCACAGCG	653	0.12779015017788858	No Hit
CTGGGTAATGGGGTGGGATGCACCGAACGATATCTACGGGGCGTTGGTGCCGATCTACG	643	0.12583318003733898	No Hit
CGGTGTAATGGGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTCCCAAGA	606	0.1185923905173055	No Hit
CAAGGTAATGGGGAAGCAGTGATATCAACGCAGAGTTATTGTAATAGGGGCTCTGTTCC	587	0.11487414725026125	No Hit
GACGGTAATGAGGTCTTACCTATAAAAGAATAATGATAAAAATTACAGGGCCAGGCGTT	571	0.1117429950253819	No Hit
CGGGGTAATGGGGGACAACAATTTCCATCTCCCTTCTTCTCTGCATAATGAAAATCTTG	569	0.11135160099727198	No Hit
ACTTGTAATGGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTG	560	0.10959032787077735	No Hit
TTTAGTAATGGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGCTCACAGT	553	0.10822044877239263	No Hit
TATTGTAATGGGGCCTAAAGAACTGAGAATCCTGGGAATTTAACCATGTCAATTCAATC	549	0.10743766071617279	No Hit
GTCAGTAATGGGGAAATATGAAAGAAAATATCTCCTTCAGTGGTCGAAATAAAACCAAA	546	0.10685056967400793	No Hit
CAAAGTAATGGGGATTTGGTTTTTAATATTCGATAAATCCATAAATAAAAATATATCAA	533	0.10430650849129344	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTCAC	15	0.006694147	53.000004	36
TCACGAA	15	0.006694147	53.000004	27
CCTTCGA	30	7.0027454E-7	53.000004	21
TCGCCAC	30	7.0027454E-7	53.000004	14
TGTCATC	15	0.006694147	53.000004	22
GAAATTA	30	7.0027454E-7	53.000004	43
GCCCCAC	15	0.006694147	53.000004	52
GTCATAT	15	0.006694147	53.000004	19
AGACTTA	15	0.006694147	53.000004	49
CTATGCA	15	0.006694147	53.000004	18
TCGTTGA	150	0.0	53.000004	40
GATACCT	15	0.006694147	53.000004	13
TCCATGG	120	0.0	53.000004	35
CTCACAC	15	0.006694147	53.000004	35
GGTACAG	30	7.0027454E-7	53.000004	21
GCCCACG	15	0.006694147	53.000004	43
TGCCCAG	15	0.006694147	53.000004	37
ACTGCAC	15	0.006694147	53.000004	46
GCGACGT	15	0.006694147	53.000004	13
CATATGG	75	0.0	53.000004	44

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content