##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1528165_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 510994 Sequences flagged as poor quality 0 Sequence length 59 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.00786506299487 31.0 30.0 31.0 27.0 33.0 2 30.960797974144512 31.0 31.0 33.0 28.0 34.0 3 31.359612833027395 31.0 31.0 34.0 30.0 34.0 4 35.16395495837524 35.0 35.0 37.0 33.0 37.0 5 35.227648465539716 37.0 35.0 37.0 33.0 37.0 6 32.24540014168464 35.0 32.0 35.0 28.0 36.0 7 33.88654661307178 35.0 33.0 35.0 30.0 37.0 8 34.70806310837309 35.0 35.0 37.0 32.0 37.0 9 36.903265008982494 39.0 36.0 39.0 33.0 39.0 10 36.6871665812123 39.0 35.0 39.0 32.0 39.0 11 37.18230742435332 39.0 37.0 39.0 34.0 39.0 12 37.293030837935476 39.0 37.0 39.0 35.0 39.0 13 36.93305792240222 39.0 37.0 39.0 33.0 39.0 14 36.625484839352325 39.0 36.0 40.0 30.0 40.0 15 35.83141485027222 38.0 34.0 40.0 30.0 40.0 16 36.070090842553924 38.0 35.0 40.0 30.0 40.0 17 36.05298692352552 38.0 35.0 40.0 29.0 40.0 18 36.22881677671362 38.0 35.0 40.0 30.0 40.0 19 36.48630316598629 39.0 35.0 40.0 30.0 40.0 20 36.46564930312293 39.0 35.0 40.0 30.0 40.0 21 36.464398799203124 38.0 35.0 40.0 30.0 40.0 22 36.43309314786475 39.0 35.0 40.0 30.0 40.0 23 36.35119786142303 38.0 35.0 40.0 30.0 40.0 24 36.34730349084334 38.0 35.0 40.0 30.0 40.0 25 36.28881552425273 39.0 35.0 40.0 30.0 40.0 26 35.8261055902809 38.0 35.0 40.0 28.0 40.0 27 35.32211532816432 38.0 34.0 40.0 27.0 40.0 28 34.730542824377586 37.0 33.0 39.0 27.0 40.0 29 34.26414595866096 35.0 33.0 39.0 26.0 40.0 30 33.86816674951174 35.0 33.0 39.0 25.0 40.0 31 33.752447191160755 35.0 33.0 39.0 24.0 40.0 32 33.59024763500158 35.0 33.0 39.0 23.0 40.0 33 33.105981674931606 35.0 33.0 39.0 21.0 40.0 34 32.741325729852015 35.0 32.0 38.0 20.0 40.0 35 32.51102165583158 35.0 31.0 38.0 19.0 40.0 36 31.925100881810746 35.0 31.0 38.0 16.0 40.0 37 31.726546691350585 35.0 30.0 38.0 15.0 40.0 38 31.273412212276465 35.0 30.0 38.0 14.0 40.0 39 30.347980602511967 35.0 25.0 38.0 10.0 40.0 40 29.474079930488422 35.0 23.0 38.0 9.0 40.0 41 28.412492123195186 33.0 20.0 37.0 8.0 40.0 42 27.532241083065554 33.0 16.0 37.0 8.0 40.0 43 26.898439120615897 33.0 15.0 37.0 8.0 40.0 44 26.354442517916063 33.0 10.0 36.0 8.0 40.0 45 26.02210593470765 33.0 10.0 36.0 8.0 40.0 46 25.596367080631083 33.0 10.0 35.0 8.0 40.0 47 25.022632359675455 31.0 10.0 35.0 8.0 39.0 48 24.676679178229097 31.0 9.0 35.0 8.0 39.0 49 24.250004892425352 30.0 9.0 35.0 8.0 39.0 50 23.77396603482624 28.0 9.0 35.0 8.0 38.0 51 23.43462349851466 27.0 9.0 35.0 8.0 38.0 52 22.84659506765246 25.0 9.0 35.0 8.0 38.0 53 22.565458694231243 24.0 9.0 35.0 8.0 38.0 54 22.26288371292031 23.0 8.0 35.0 8.0 38.0 55 21.927654727844164 23.0 8.0 35.0 8.0 37.0 56 21.447913282739133 21.0 8.0 34.0 8.0 36.0 57 20.858608907345292 20.0 8.0 34.0 8.0 36.0 58 20.3510178201701 18.0 8.0 33.0 8.0 35.0 59 19.69499250480436 17.0 8.0 33.0 7.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 0.0 12 0.0 13 5.0 14 8.0 15 63.0 16 183.0 17 604.0 18 1486.0 19 2868.0 20 5170.0 21 7985.0 22 11762.0 23 16022.0 24 20992.0 25 27049.0 26 33377.0 27 36944.0 28 31620.0 29 23868.0 30 21625.0 31 23539.0 32 27398.0 33 31898.0 34 38259.0 35 38820.0 36 41945.0 37 39712.0 38 26424.0 39 1367.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.831524440600084 23.194988590864078 37.263646931275126 4.709840037260712 2 28.474306939024725 21.056411621271483 33.59237094760408 16.87691049209971 3 21.944288974038834 20.774999315060448 36.93800709988767 20.342704611013048 4 25.49188444482714 21.450154013550062 33.26673111621663 19.79123042540617 5 100.0 0.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 0.0 100.0 0.0 0.0 8 0.0 100.0 0.0 0.0 9 0.0 0.0 100.0 0.0 10 100.0 0.0 0.0 0.0 11 98.66886891039816 1.006078349256547 0.14383730533039527 0.18121543501489254 12 97.69566765950285 1.485340336677143 0.3585169297486859 0.4604750740713199 13 84.73074047836178 11.334379660035147 2.2518855407304192 1.6829943208726523 14 28.94534965185501 52.18123891865658 12.242413805250159 6.630997624238249 15 20.31139308876425 59.26762349460072 14.070615310551593 6.350368106083437 16 28.150428380763763 48.67102157755277 14.076486220973242 9.102063820710224 17 14.85301197274332 51.76636124886006 14.407214174726121 18.973412603670493 18 13.611314418564602 58.66448529728333 16.07181297627761 11.652387307874456 19 18.822725902848177 54.33214480013464 20.203368337005916 6.641760960011273 20 14.619936829003862 52.592594042200105 21.921588120408458 10.865881008387573 21 19.636825481316805 50.68689651933291 12.946335964805849 16.729942034544436 22 19.701796889983054 55.17657741578179 14.731679824029245 10.389945870205912 23 15.098220331354185 53.2951463226574 19.019988493015575 12.586644852972833 24 15.338340567599621 58.64491559587783 12.787429989393223 13.22931384712932 25 15.061624989725908 55.009452165778846 17.935631338137043 11.993291506358196 26 13.566891196374126 57.114369248954 13.138314735593765 16.18042481907811 27 13.151817829563555 62.053370489673064 11.385847974731602 13.408963706031773 28 14.293709906574245 59.793069977338284 12.732830522471888 13.18038959361558 29 18.47360242977413 54.95035166753426 12.91326316943056 13.662782733261055 30 21.55543900711163 57.59441402443082 10.249239717100396 10.600907251357159 31 16.112713652215092 61.23183442467035 10.095617561067254 12.559834362047303 32 15.262801520174405 64.45829109539447 11.253948187258558 9.024959197172569 33 17.772811422443315 63.04672853301604 11.221462482925435 7.958997561615205 34 13.942433766345594 65.4813950848738 11.77880757895396 8.797363569826652 35 17.367914300363605 61.75806369546414 13.50231118173599 7.371710822436271 36 13.20876566065355 61.84984559505591 15.262801520174405 9.678587224116136 37 14.00623099292751 62.18683585325855 15.110940637267756 8.695992516546184 38 15.164953013146926 59.55236265005068 15.615643236515497 9.667041100286893 39 16.73561724795203 57.271513951240124 14.686865207810659 11.306003592997179 40 20.657581106627475 55.133719769703745 13.51777124584633 10.690927877822439 41 21.380877270574604 53.16187665608598 14.492733769868138 10.964512303471272 42 20.34877121844875 52.19885164992153 15.77200515074541 11.680371980884315 43 20.919423711433012 53.629983913705445 13.72325311060404 11.727339264257505 44 20.95093093069586 50.21702798858695 16.163203481841272 12.668837598875914 45 23.329823833547948 45.547110142193446 17.118009213415423 14.00505681084318 46 27.472533924077386 46.74986399057523 13.571392227697391 12.206209857649993 47 27.730462588601824 47.042431026587394 13.287240163289587 11.939866221521193 48 25.549223669945242 48.23226887204155 13.297416408020446 12.921091049992759 49 22.926296590566622 51.90021800647365 10.66529156898124 14.508193833978481 50 21.206902625079746 51.03054047601342 13.035378106200856 14.727178792705981 51 20.596719335256385 52.60472725707152 12.202100220354838 14.596453187317268 52 20.41315553607283 51.53406889317683 13.126377217736412 14.926398353013932 53 19.485160295424212 49.58257826902077 14.263768263423838 16.66849317213118 54 19.007659581130113 50.349710564116215 14.600171430584313 16.042458424169364 55 18.843665483352055 48.158491097742825 15.763981573169156 17.233861845735955 56 20.333115457324354 44.9985714117974 16.56829630093504 18.100016829943208 57 20.689675416932488 43.612058067218015 17.663808185614705 18.0344583302348 58 20.26246883525051 42.128479003667366 19.34778099155763 18.261271169524495 59 21.30416404106506 38.892433179254546 20.426854327056677 19.37654845262371 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 5.0 5 10.0 6 325.0 7 640.0 8 1439.0 9 4263.5 10 6289.0 11 6452.5 12 6616.0 13 5954.0 14 4094.5 15 2897.0 16 2987.5 17 3078.0 18 3568.5 19 5225.0 20 6391.0 21 8618.5 22 10846.0 23 13502.5 24 16159.0 25 19504.5 26 25800.0 27 28750.0 28 30761.5 29 32773.0 30 33752.0 31 34363.5 32 33996.0 33 35306.5 34 36617.0 35 32658.0 36 28657.0 37 28615.0 38 25952.5 39 23290.0 40 22113.0 41 20936.0 42 21777.5 43 22603.0 44 22587.0 45 21282.5 46 19978.0 47 19786.5 48 17079.0 49 14563.0 50 13634.0 51 12705.0 52 12488.0 53 10587.5 54 8904.0 55 8780.5 56 8657.0 57 6011.0 58 2778.5 59 2192.0 60 2634.5 61 3077.0 62 2999.5 63 2922.0 64 1790.5 65 522.5 66 386.0 67 341.5 68 297.0 69 259.0 70 164.0 71 107.0 72 100.0 73 93.0 74 55.0 75 11.0 76 5.0 77 2.5 78 0.0 79 0.5 80 1.0 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 53 0.0 54 0.0 55 0.0 56 0.0 57 0.0 58 0.0 59 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 59 510994.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.68913516893754 #Duplication Level Percentage of deduplicated Percentage of total 1 94.00913690952441 62.6938803847443 2 2.7475554796741486 3.664641975362887 3 0.9053598782802995 1.811330018975032 4 0.443386718095767 1.1827630710080084 5 0.2988238100246079 0.9964150729213994 6 0.2023169030387676 0.8095403576227922 7 0.16533283408203145 0.7718132603972084 8 0.12510179503186905 0.6674344415005626 9 0.09029090237992655 0.541927997400624 >10 0.9047817374414705 12.276535731755986 >50 0.05055651695692609 2.2926678598386827 >100 0.04819129908023986 6.796770343724587 >500 0.006799999256720957 3.202794658373815 >1k 0.0023652171327725067 2.291484826374075 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ACTTGTAATGGGGGCAGTGGTGTGATCTAGGCTCACTGCAACCTCTGCCTCCCAGCTTC 1889 0.3696716595498186 No Hit GGGAGTAATGGGGGATCACCTACGGTCAGGAGTTTGAGACCAGCCTGACCAACATGGTG 1725 0.3375773492448052 No Hit TACGGTAATGGGGCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGG 1601 0.31331091950199025 No Hit TGGTGTAATGGGGTACCATGCAAGTAACTACAAGAAAAGATGGCTATAAAAACAAAATA 1441 0.2819993972531967 No Hit TGCAGTAATGGGGGGCGCGGTGGCTCATGCCTGTAATCCCAGCACTTTGGGACGCCGAT 1343 0.26282108987581065 No Hit AAGTGTAATGGGGGTGAAACCCCGTCTCTACTAAAAATACAAAAAATTAGGCAGGCGGG 1262 0.24696963173735897 No Hit GATCGTAATGGGGGAGGAGCCTGGTGAGCTACAGTCCATGGGGTCGCAATAGTCGGATA 1202 0.23522781089406136 No Hit ATCAGTAATGGGCACACCACCTTATCAAATAAAATATTCTTATAAAACGGCAGCATTCT 1159 0.22681283928969814 No Hit AGAGGTAATGGGGGAGATTATTAGCAAAAGCATGTTAAGAATTAATTACCGAATTCATA 1000 0.19569701405495957 No Hit TGCGGTAATGGGGTGATCAAGACCTTTAGGAGAAGTGAGATGGGACATAGAGCCAAGAA 958 0.18747773946465124 No Hit AATCGTAATGGAGAACGGTACCCAAACTTTCGCGGAGGTACTCACCATCCAGATGATAT 909 0.17788858577595823 No Hit AATAGTAATGGGGAAGAGTGGTATCAACGCAGATTACAGTAATGGGGAACTATCTAATA 880 0.1722133723683644 No Hit GCAGGTAATGGGGAATGCTAAGAGTTCAGCTTGCCAGTTCATTTTTCTGTCCTATGTTA 855 0.16732094701699043 No Hit CTAGGTAATGGGGAAAGAAAATTTTCCCTCCCCTATACTCTTTTTATAACACAGAATAT 835 0.16340700673589123 No Hit TTAAGTAATGGGGGCAACGTGTGTACTCACAATAAGTTTTTTTATATTCACTCAATGAG 832 0.16281991569372634 No Hit GTTCGTAATGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGA 789 0.1544049440893631 No Hit CACTGTAATGGGGAGGATGTAGGCCAAAAGAGGCTCTGTCCAGCCTGACCAACATGGAG 766 0.14990391276609902 No Hit ACCGGTAATGGGGTGGGCGGACTCGGCTTCGTGATGACACTGCTCTACACCAAAAAAAA 722 0.1412932441476808 No Hit CACAGTAATGGGGGTTCTAGGGTACATGTGCACAACGTGCAGGTTTGTTACATATGTAT 672 0.13150839344493281 No Hit TAATGTAATGGGGGATATATTCCCAGTAGTGAGATTGCTGGATCATATGGTAGTTCCAT 656 0.12837724122005345 No Hit TGGGGTAATGGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATGCACAGCG 653 0.12779015017788858 No Hit CTGGGTAATGGGGTGGGATGCACCGAACGATATCTACGGGGCGTTGGTGCCGATCTACG 643 0.12583318003733898 No Hit CGGTGTAATGGGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTCCCAAGA 606 0.1185923905173055 No Hit CAAGGTAATGGGGAAGCAGTGATATCAACGCAGAGTTATTGTAATAGGGGCTCTGTTCC 587 0.11487414725026125 No Hit GACGGTAATGAGGTCTTACCTATAAAAGAATAATGATAAAAATTACAGGGCCAGGCGTT 571 0.1117429950253819 No Hit CGGGGTAATGGGGGACAACAATTTCCATCTCCCTTCTTCTCTGCATAATGAAAATCTTG 569 0.11135160099727198 No Hit ACTTGTAATGGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTG 560 0.10959032787077735 No Hit TTTAGTAATGGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGCTCACAGT 553 0.10822044877239263 No Hit TATTGTAATGGGGCCTAAAGAACTGAGAATCCTGGGAATTTAACCATGTCAATTCAATC 549 0.10743766071617279 No Hit GTCAGTAATGGGGAAATATGAAAGAAAATATCTCCTTCAGTGGTCGAAATAAAACCAAA 546 0.10685056967400793 No Hit CAAAGTAATGGGGATTTGGTTTTTAATATTCGATAAATCCATAAATAAAAATATATCAA 533 0.10430650849129344 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.12270202781245963 0.0 0.0 0.0 0.0 12 0.24012023624543535 0.0 0.0 0.0 0.0 13 0.5385581826792487 0.0 0.0 0.0 0.0 14 1.1824013589200657 0.0 0.0 0.0 0.0 15 1.684559896985092 0.0 0.0 0.0 0.0 16 1.993565482177873 0.0 0.0 0.0 0.0 17 2.1608864291948633 0.0 0.0 0.0 0.0 18 2.3726305984023295 0.0 0.0 0.0 0.0 19 2.579873736286532 0.0 0.0 0.0 0.0 20 2.773809477214997 0.0 0.0 0.0 0.0 21 2.9828138882256936 0.0 0.0 0.0 0.0 22 3.2145191528667656 0.0 0.0 0.0 0.0 23 3.425089139989902 0.0 0.0 0.0 0.0 24 3.6675577404039967 0.0 0.0 0.0 0.0 25 3.9354669526452364 0.0 0.0 0.0 0.0 26 4.228425382685511 0.0 0.0 0.0 0.0 27 4.510620476952763 0.0 0.0 0.0 0.0 28 4.794576844346509 0.0 0.0 0.0 0.0 29 5.111410310101489 0.0 0.0 0.0 0.0 30 5.477559423398318 0.0 0.0 0.0 0.0 31 5.772279126565087 0.0 0.0 0.0 0.0 32 5.985001780842828 0.0 0.0 0.0 0.0 33 6.159367820365797 0.0 0.0 0.0 0.0 34 6.335690830029315 0.0 0.0 0.0 0.0 35 6.473461527924007 0.0 0.0 0.0 0.0 36 6.600860284073786 0.0 0.0 0.0 0.0 37 6.72688916112518 0.0 0.0 0.0 0.0 38 6.863877070963651 0.0 0.0 0.0 0.0 39 7.0038004360129476 0.0 0.0 0.0 0.0 40 7.153508651764992 0.0 0.0 0.0 0.0 41 7.299694321264046 0.0 0.0 0.0 0.0 42 7.448032657917706 0.0 0.0 0.0 0.0 43 7.602241904993013 0.0 0.0 0.0 0.0 44 7.762909153532135 0.0 0.0 0.0 0.0 45 7.907724943932806 0.0 0.0 0.0 0.0 46 8.045691338841552 0.0 0.0 0.0 0.0 47 8.191681311326551 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGTCAC 15 0.006694147 53.000004 36 TCACGAA 15 0.006694147 53.000004 27 CCTTCGA 30 7.0027454E-7 53.000004 21 TCGCCAC 30 7.0027454E-7 53.000004 14 TGTCATC 15 0.006694147 53.000004 22 GAAATTA 30 7.0027454E-7 53.000004 43 GCCCCAC 15 0.006694147 53.000004 52 GTCATAT 15 0.006694147 53.000004 19 AGACTTA 15 0.006694147 53.000004 49 CTATGCA 15 0.006694147 53.000004 18 TCGTTGA 150 0.0 53.000004 40 GATACCT 15 0.006694147 53.000004 13 TCCATGG 120 0.0 53.000004 35 CTCACAC 15 0.006694147 53.000004 35 GGTACAG 30 7.0027454E-7 53.000004 21 GCCCACG 15 0.006694147 53.000004 43 TGCCCAG 15 0.006694147 53.000004 37 ACTGCAC 15 0.006694147 53.000004 46 GCGACGT 15 0.006694147 53.000004 13 CATATGG 75 0.0 53.000004 44 >>END_MODULE