FastQCFastQC Report
Fri 17 Jun 2016
SRR1528164_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528164_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences411677
Sequences flagged as poor quality0
Sequence length52
%GC38

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTAGCATGGGGGCCACGGCGGATGGATCACAAGGTCAGGAGTTCAAGAC29060.7058932123970977No Hit
TGAGTAGCATGGAGCCTGTAATCCCAGTACTTTGGGAGGCTGAGGTGTGCGG22150.5380431746247665No Hit
ATAATAGCATGGGATCCCTGCACTTTGGGAGGCCGAGGTCGGCAGATCATGA21030.5108373797904668No Hit
GAGTTAGCATGGGGTTTGAAATTGGATATATGGATTTATTGAATTGAAATAA18170.44136543941002293No Hit
TAAGTAGCATGGGGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCG17790.43213490187695697No Hit
GCTCTAGCATGGGGGTCTTGGGCTGATTTTGCTAGCGCTGGAGTTGATGGTG17200.41780327781245974No Hit
AAAATAGCATGGGAAGACTGGAGGAGCACTATCTACAGAGCAGCGCCTGCGG15820.38428185203448334No Hit
TGTATAGCATGGGAATCAAAAAATACACTACAGGTTTAGGCCAGGCATGGTG15070.3660636858508005No Hit
GTATTAGCATGGGGTGGGCTAGCATGGGAACTACAGGTGCATGCCACCTCAC14700.35707605720018365No Hit
GGTTTAGCATGGGAAGCAGTGGTATCAATGCAGAGTTTTTTAGCATGGGTTG13270.3223400870099617No Hit
CGGATAGCATGGGTAATCTTTTATGGTTAAAAATATCTTTTATAGTTATAAA13190.32039681595036884No Hit
GTGTTAGCATGGGCCATTGTTGATGACTAAGGCAAAAAGATTTTTCTTTGTA11460.2783735792866738No Hit
TTTCTAGCATGGGATGTAGCATCTTTGTCGAGATAAAGAAAACAGTCCCCAT11120.2701146772834042No Hit
CGCATAGCATGGGAGACGCTGTCATTTAGTACATGAACTAAAGATATAATCC10840.2633132285748293No Hit
GGAGTAGCATGGGGGACCGGCCCTGAGCTGCCCTCGGGATCAAGCCAGCCCG10820.2628274108099311No Hit
AGTCTAGCATGGGGGAATTCGCCCTAAGTTCCGGTCCGCAATTTCGTTTCGT10750.26112704863278735No Hit
GACGTAGCATGGGGCGTTAGGTGCCGCAATTGGTGCGGTAACCGGGGTGATT10700.25991250422054185No Hit
AGTTTAGCATGGGGTTAACGCCGCCTGAATGGACTCCTGCATCACTTCGCCG10470.25432559992421244No Hit
AAAGTAGCATGGGGTTCCATCCCTGATTCAGGAAGATCCCACATGCCTCGGG10330.250924875569925No Hit
ATCATAGCATGGGAGAAGGGAATGACAGAGGATGAGATGGTTGGATGCATCA9900.24047979362461347No Hit
ATGCTAGCATGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTC9840.23902234032991881No Hit
ATTATAGCATGGGGGGTTCAAATGACTCTCCTGCCTCAGCCTCCTGAGTAGT9340.2268768962074636No Hit
GAGTTAGCATGGGCTCTCAGACCACAGTGCAATCAAACTAGAAGTCAGGATT8560.20793000337643347No Hit
TTTATAGCATGGGGTTGCTTAGCATGGGCAGATAATTTTTATGGCGAAACCC8350.20282891684500226No Hit
GGCTTAGCATGGGCAACAAGGCGAAACCCCGTCTCTACAAAATATAGAAAAA8290.20137146355030766No Hit
TAAGTAGCATGGGATGGTGGCATTTACGAAACGGGTGATCGCGAATATACAT8240.20015691913806213No Hit
GGAGTAGCATGGGGGTTGACAGAGTGAGACTGTCTCAAAAAACTAAAAACTA8130.19748492143112198No Hit
GCCTTAGCATGGGGTTTTCAACAACTTCACGGAAGAATAGGTTCGTGGAGCT7990.1940841970768345No Hit
AACATAGCATGGGGTTTGCAGGCTTAACTCCGGCCGGGGTTCCTCGCTCGAG7890.19165510825234344No Hit
GAATTAGCATGGGGTAGCTCTATGGAAAAAGACTATTTGAGAATTAGTAGTA7810.18971183719275064No Hit
GATCTAGCATGGGTCTCATAGCATGGGTAGGAGGAGTTGCGAACAAGCAGCA7660.18606820395601406No Hit
GAACTAGCATGGGGGGGAATTCAGATGTATATATGATGTCCTTGGACGGGGT7400.17975257301233732No Hit
TCGGTAGCATGGGTACATAAACACCTATAGATATAATTAGGTATAAATCTAA7300.1773234841878463No Hit
GATCTAGCATGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAG7180.17440857759845704No Hit
CTGTTAGCATGGGTGAGGAAACAGGTACAGAGAAGTTATAGGGCTTATATAA7130.17319403318621152No Hit
GGGTTAGCATGGGAAGTGCTCGCCGAACAGAACGCAGATGTCCGCCGCGATC7120.17295112430376242No Hit
CTAGTAGCATGGGGATAAACGCTATCACATTGTTGAAGGCGCAAACCATATG7100.17246530653886422No Hit
GGTCTAGCATGGGGTCCATTTTTTTAAGAGCAGAATGCTTTCTGGAAATGAT6850.1663925844776366No Hit
CCCATAGCATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT6810.16542094894784018No Hit
TGCGTAGCATGGGGTACAGCAGCTGCGCGTCTTTACGCACCACAAAGTGGGT6540.15886240912171434No Hit
ATTCTAGCATGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAA6230.1513322337657921No Hit
TGGATAGCATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT6150.14938896270619928No Hit
TAATTAGCATGGAGGCATGGTGGCATGCACCTGTAGTTGCAGCCACTCAGGA6020.1462311472343609No Hit
GACTTAGCATGGATGAAATCAACACACAGCTTTCCACTGTCATTCGCCATAG5850.14210169623272614No Hit
ACATTAGCATGGGGTCTCTCTTCTTCTCATGAATGTTGATTACATGGTCATA5710.1387009718784387No Hit
TATGTAGCATGGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACAT5670.13772933634864226No Hit
TTTGTAGCATGGGAGTAATGGCGGTGGTGAGCTCGGTGGTGCAGCTGGAGTC5510.13384279422945658No Hit
AATATAGCATGGGGACGTGTCAGTACTATGTGTGAGTGCTCCGCCCTAGTGC5390.13092788764006733No Hit
GAATTAGCATGGGGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAA5350.12995625211027093No Hit
TTATTAGCATGGGTGTTGCATGATGCTAAGGTTTGGGCTTCCATTGATCCCG5310.1289846165804745No Hit
GTACTAGCATGGGATCATGTCACTTTCTCAGATGTTTCCTCATCTGTCAAAT5310.1289846165804745No Hit
AATTTAGCATGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAA5280.12825588993312717No Hit
TTAGTAGCATGGGATGCCTAGATGTGATACACGTTTCTGGAAACTGCCTCGT5100.1238835300490433No Hit
GTCGTAGCATGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG5050.12266898563679779No Hit
TCTTTAGCATGGGGGAGCTCAAAGAATGCAACAATATATAAACAAATCATCA4990.12121153234210315No Hit
ATCGTAGCATGGGCACACAGCTTTCCACTGTCATTCGCCATAGAAGCCGATA4770.11586753692822285No Hit
AATTTAGCATGGGATGTCATTTTGGACAACACTGCTTGTCAAACGCTCCTCA4710.11441008363352821No Hit
AAACTAGCATGGGGGTGCAGGGATGGTGTTTTCAGTCTTCTTTCATGAGTGA4630.1124668125739354No Hit
CATTTAGCATGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGC4620.11222390369148628No Hit
TCGCTAGCATGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATG4610.11198099480903718No Hit
GGATTAGCATGGGCAGAGTCGCCTAGCATGGGCCAAATCAGTAAATTTGTGC4610.11198099480903718No Hit
TGGTTAGCATGGGTCCAGCATCCGTTTATGATTAAAACTTTCAGCAAAAATC4560.11076645039679166No Hit
ACTGTAGCATGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGC4540.11028063263189346No Hit
CTATTAGCATGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGC4490.10906608821964793No Hit
CGTATAGCATGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTT4480.10882317933719882No Hit
TTTGTAGCATGGGAGGCTGTACCTTGCAAAGCCACAGGGGCAGAGCTGCATA4480.10882317933719882No Hit
TATGTAGCATGGGGTATAGATGAACAAGTGAAGCACTGAGGATTTTTAGGCA4460.10833736157230062No Hit
AAGGTAGCATGGCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACT4450.10809445268985152No Hit
TTTGTAGCATGGGAAACCCCCATGGGTTATAACGAAGTGACCTTCCTCTCTG4400.10687990827760599No Hit
TTTTTAGCATGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG4310.10469372833556406No Hit
TTTGTAGCATGGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGA4310.10469372833556406No Hit
TTTTTAGCATGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGC4250.10323627504086942No Hit
TTTATAGCATGGGTGTGAATTGTTTCTTGGTTCCAGGTGTTTAAGGAAACTT4220.1025075483935221No Hit
GATATAGCATGGCTTGGACAAGAATGTTCAGAGCAGTTTTATTCAGACTAGC4160.10105009509882748No Hit
AACTTAGCATGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATG4140.10056427733392927No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGCA351.0174335E-746.00000439
GGATGTC700.046.00000412
TCCTTGG700.046.00000439
CAAGACA351.0174335E-746.00000430
CAAGAAT351.0174335E-746.00000419
TTGCAAA351.0174335E-746.00000424
TCCCAAG351.0174335E-746.00000427
CGTTAGG1400.046.00000415
TATATAT700.046.00000428
AGCCACA351.0174335E-746.00000430
TGTCCTT700.046.00000437
TATGCAT351.0174335E-746.00000440
CGCTCCT700.046.00000444
TTTTCAG351.0174335E-746.00000429
TGTTCAG351.0174335E-746.00000425
CCTGAGG351.0174335E-746.00000444
CCTTGCA351.0174335E-746.00000422
CATTCAT351.0174335E-746.00000422
GTATATA700.046.00000427
ACCTGAG351.0174335E-746.00000443
Produced by FastQC (version 0.11.5)