FastQCFastQC Report
Fri 17 Jun 2016
SRR1528163_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528163_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences409642
Sequences flagged as poor quality0
Sequence length52
%GC38

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTAGCATGGGGGCCACGGCGGATGGATCACAAGGTCAGGAGTTCAAGAC29270.7145263425137072No Hit
ATAATAGCATGGGATCCCTGCACTTTGGGAGGCCGAGGTCGGCAGATCATGA21570.5265573354294726No Hit
TGAGTAGCATGGAGCCTGTAATCCCAGTACTTTGGGAGGCTGAGGTGTGCGG21250.518745636433764No Hit
GAGTTAGCATGGGGTTTGAAATTGGATATATGGATTTATTGAATTGAAATAA18440.45014915462769933No Hit
TAAGTAGCATGGGGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCG18080.4413609932575273No Hit
GCTCTAGCATGGGGGTCTTGGGCTGATTTTGCTAGCGCTGGAGTTGATGGTG16190.3952231460641243No Hit
AAAATAGCATGGGAAGACTGGAGGAGCACTATCTACAGAGCAGCGCCTGCGG15700.38326148197694576No Hit
GTATTAGCATGGGGTGGGCTAGCATGGGAACTACAGGTGCATGCCACCTCAC14680.3583616914281251No Hit
TGTATAGCATGGGAATCAAAAAATACACTACAGGTTTAGGCCAGGCATGGTG14640.3573852290536615No Hit
CGGATAGCATGGGTAATCTTTTATGGTTAAAAATATCTTTTATAGTTATAAA13170.3215002367921258No Hit
GGTTTAGCATGGGAAGCAGTGGTATCAATGCAGAGTTTTTTAGCATGGGTTG11830.2887887472475967No Hit
GTGTTAGCATGGGCCATTGTTGATGACTAAGGCAAAAAGATTTTTCTTTGTA11150.2721888868817162No Hit
TTTCTAGCATGGGATGTAGCATCTTTGTCGAGATAAAGAAAACAGTCCCCAT11110.2712124245072527No Hit
AGTTTAGCATGGGGTTAACGCCGCCTGAATGGACTCCTGCATCACTTCGCCG10810.263888956698776No Hit
GGAGTAGCATGGGGGACCGGCCCTGAGCTGCCCTCGGGATCAAGCCAGCCCG10520.2568096044839152No Hit
GACGTAGCATGGGGCGTTAGGTGCCGCAATTGGTGCGGTAACCGGGGTGATT10450.255100795328604No Hit
AGTCTAGCATGGGGGAATTCGCCCTAAGTTCCGGTCCGCAATTTCGTTTCGT10250.2502184834562862No Hit
AAAGTAGCATGGGGTTCCATCCCTGATTCAGGAAGATCCCACATGCCTCGGG10120.24704498073927966No Hit
CGCATAGCATGGGAGACGCTGTCATTTAGTACATGAACTAAAGATATAATCC10040.24509205599035255No Hit
ATCATAGCATGGGAGAAGGGAATGACAGAGGATGAGATGGTTGGATGCATCA9760.23825681936910764No Hit
ATGCTAGCATGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTC9610.23459508546486932No Hit
ATTATAGCATGGGGGGTTCAAATGACTCTCCTGCCTCAGCCTCCTGAGTAGT9550.233130391903174No Hit
GCCTTAGCATGGGGTTTTCAACAACTTCACGGAAGAATAGGTTCGTGGAGCT8190.19993067117141308No Hit
GGAGTAGCATGGGGGTTGACAGAGTGAGACTGTCTCAAAAAACTAAAAACTA8110.19797774642248597No Hit
TAAGTAGCATGGGATGGTGGCATTTACGAAACGGGTGATCGCGAATATACAT8080.19724539964163829No Hit
AACATAGCATGGGGTTTGCAGGCTTAACTCCGGCCGGGGTTCCTCGCTCGAG8020.195780706079943No Hit
GAATTAGCATGGGGTAGCTCTATGGAAAAAGACTATTTGAGAATTAGTAGTA7840.19138662539485699No Hit
GGCTTAGCATGGGCAACAAGGCGAAACCCCGTCTCTACAAAATATAGAAAAA7810.19065427861400933No Hit
TTTATAGCATGGGGTTGCTTAGCATGGGCAGATAATTTTTATGGCGAAACCC7770.18967781623954574No Hit
GAGTTAGCATGGGCTCTCAGACCACAGTGCAATCAAACTAGAAGTCAGGATT7650.18674842911615508No Hit
GATCTAGCATGGGTCTCATAGCATGGGTAGGAGGAGTTGCGAACAAGCAGCA7600.18552785114807563No Hit
GGGTTAGCATGGGAAGTGCTCGCCGAACAGAACGCAGATGTCCGCCGCGATC7560.18455138877361207No Hit
CTGTTAGCATGGGTGAGGAAACAGGTACAGAGAAGTTATAGGGCTTATATAA7060.17234560909281765No Hit
GGTCTAGCATGGGGTCCATTTTTTTAAGAGCAGAATGCTTTCTGGAAATGAT6990.1706367999375064No Hit
CTAGTAGCATGGGGATAAACGCTATCACATTGTTGAAGGCGCAAACCATATG6950.16966033756304286No Hit
TCGGTAGCATGGGTACATAAACACCTATAGATATAATTAGGTATAAATCTAA6940.16941622196942696No Hit
GAACTAGCATGGGGGGGAATTCAGATGTATATATGATGTCCTTGGACGGGGT6880.16795152840773162No Hit
CCCATAGCATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT6860.16746329722049985No Hit
TGCGTAGCATGGGGTACAGCAGCTGCGCGTCTTTACGCACCACAAAGTGGGT6450.1574545578822484No Hit
GATCTAGCATGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAG6200.15135166804185118No Hit
TAATTAGCATGGAGGCATGGTGGCATGCACCTGTAGTTGCAGCCACTCAGGA6070.14817816532484462No Hit
TGGATAGCATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT5750.14036646632913619No Hit
ATTCTAGCATGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAA5630.1374370792057455No Hit
ACATTAGCATGGGGTCTCTCTTCTTCTCATGAATGTTGATTACATGGTCATA5550.1354841544568184No Hit
GACTTAGCATGGATGAAATCAACACACAGCTTTCCACTGTCATTCGCCATAG5540.13524003886320252No Hit
TTAGTAGCATGGGATGCCTAGATGTGATACACGTTTCTGGAAACTGCCTCGT5450.1330429985206595No Hit
GAATTAGCATGGGGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAA5350.1306018425845006No Hit
AATATAGCATGGGGACGTGTCAGTACTATGTGTGAGTGCTCCGCCCTAGTGC5170.12620776189941463No Hit
AATTTAGCATGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAA5160.12596364630579873No Hit
AAGGTAGCATGGCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACT5140.12547541511856694No Hit
GTACTAGCATGGGATCATGTCACTTTCTCAGATGTTTCCTCATCTGTCAAAT5120.12498718393133518No Hit
TATGTAGCATGGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACAT5100.1244989527441034No Hit
ATCGTAGCATGGGCACACAGCTTTCCACTGTCATTCGCCATAGAAGCCGATA5070.12376660596325573No Hit
TCTTTAGCATGGGGGAGCTCAAAGAATGCAACAATATATAAACAAATCATCA5040.12303425918240805No Hit
GTCGTAGCATGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG5010.12230191240156038No Hit
TTTGTAGCATGGGAGTAATGGCGGTGGTGAGCTCGGTGGTGCAGCTGGAGTC4960.12108133443348094No Hit
TTATTAGCATGGGTGTTGCATGATGCTAAGGTTTGGGCTTCCATTGATCCCG4910.1198607564654015No Hit
TCGCTAGCATGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATG4890.11937252527816972No Hit
AAACTAGCATGGGGGTGCAGGGATGGTGTTTTCAGTCTTCTTTCATGAGTGA4720.11522256018669962No Hit
CGTATAGCATGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTT4710.11497844459308372No Hit
ACTGTAGCATGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGC4610.11253728865692483No Hit
TTTTTAGCATGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGC4580.11180494187607716No Hit
CTATTAGCATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT4540.1108284795016136No Hit
AATTTAGCATGGGATGTCATTTTGGACAACACTGCTTGTCAAACGCTCCTCA4540.1108284795016136No Hit
TTTGTAGCATGGGAAACCCCCATGGGTTATAACGAAGTGACCTTCCTCTCTG4460.10887555475268648No Hit
AACTTAGCATGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATG4450.1086314391590706No Hit
AGTCTAGCATGGGGAGTTCTGTAGCATGGGGGGAATTCTCCAGATTACTTCC4390.10716674559737528No Hit
CTATTAGCATGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGC4380.10692263000375939No Hit
CATTTAGCATGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGC4340.10594616762929583No Hit
TGGTTAGCATGGGTCCAGCATCCGTTTATGATTAAAACTTTCAGCAAAAATC4340.10594616762929583No Hit
TATGTAGCATGGGGTATAGATGAACAAGTGAAGCACTGAGGATTTTTAGGCA4290.10472558966121638No Hit
TTTGTAGCATGGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGA4280.1044814740676005No Hit
TTTGTAGCATGGGAGGCTGTACCTTGCAAAGCCACAGGGGCAGAGCTGCATA4230.10326089609952105No Hit
GATATAGCATGGCTTGGACAAGAATGTTCAGAGCAGTTTTATTCAGACTAGC4140.10106385575697804No Hit
GGATTAGCATGGGCAGAGTCGCCTAGCATGGGCCAAATCAGTAAATTTGTGC4140.10106385575697804No Hit
AAATTAGCATGACCTCACCAAATGAACTAAAGAAACCACCAGAGACCAGTCC4110.10033150897613037No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGCA351.01741534E-746.00000439
TTTGAGT351.01741534E-746.00000440
GGATGTC351.01741534E-746.00000412
ACACGTT700.046.00000429
TGTTGCA700.046.00000414
ACTGCCT700.046.00000443
TTCATCT351.01741534E-746.00000419
CATGACC351.01741534E-746.0000048
GTTTGAG351.01741534E-746.00000439
GTTTGAA2800.046.00000414
CATGAAT700.046.00000428
CTGAGCA351.01741534E-746.00000419
GAAATTG2800.046.00000418
TATATGG2800.046.00000427
CTTTACG700.046.00000431
TGACCTC351.01741534E-746.00000410
ACTGAGG700.046.00000435
AAATCAT700.046.00000444
AGGATTT700.046.00000439
TCCTAGT351.01741534E-746.00000446
Produced by FastQC (version 0.11.5)