FastQCFastQC Report
Fri 17 Jun 2016
SRR1528162_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528162_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390579
Sequences flagged as poor quality0
Sequence length59
%GC38

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTAGCATGGGGGCCACGGCGGATGGATCACAAGGTCAGGAGTTCAAGACCAGCCTG26210.6710550234395602No Hit
ATAATAGCATGGGATCCCTGCACTTTGGGAGGCCGAGGTCGGCAGATCATGAAGTCGGG17580.4501010038942186No Hit
TGAGTAGCATGGAGCCTGTAATCCCAGTACTTTGGGAGGCTGAGGTGTGCGGATCACCT17120.43832361698913663No Hit
GAGTTAGCATGGGGTTTGAAATTGGATATATGGATTTATTGAATTGAAATAATGTAAAT16500.4224497476822871No Hit
TAAGTAGCATGGGGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCC14920.38199698396483167No Hit
GCTCTAGCATGGGGGTCTTGGGCTGATTTTGCTAGCGCTGGAGTTGATGGTGCAGGCCG13490.3453846724990335No Hit
AAAATAGCATGGGAAGACTGGAGGAGCACTATCTACAGAGCAGCGCCTGCGGTGGCCAA12450.3187575368875439No Hit
GTATTAGCATGGGGTGGGCTAGCATGGGAACTACAGGTGCATGCCACCTCACGCAGCTA12300.31491708463588675No Hit
TGTATAGCATGGGAATCAAAAAATACACTACAGGTTTAGGCCAGGCATGGTGGCTTATG11810.30237160728047335No Hit
CGGATAGCATGGGTAATCTTTTATGGTTAAAAATATCTTTTATAGTTATAAAAATATCT11120.28470552692285045No Hit
GGTTTAGCATGGGAAGCAGTGGTATCAATGCAGAGTTTTTTAGCATGGGTTGAGACCAG10690.27369623046809993No Hit
GTGTTAGCATGGGCCATTGTTGATGACTAAGGCAAAAAGATTTTTCTTTGTATCTCCAG10630.27216004956743706No Hit
TTTCTAGCATGGGATGTAGCATCTTTGTCGAGATAAAGAAAACAGTCCCCATCCCAATG9660.2473251250067208No Hit
CGCATAGCATGGGAGACGCTGTCATTTAGTACATGAACTAAAGATATAATCCTGTTTAC9540.24425276320539507No Hit
ATCATAGCATGGGAGAAGGGAATGACAGAGGATGAGATGGTTGGATGCATCACGGACTC9200.23554773810163884No Hit
AGTTTAGCATGGGGTTAACGCCGCCTGAATGGACTCCTGCATCACTTCGCCGAGCGAAC8740.22377035119655692No Hit
GACGTAGCATGGGGCGTTAGGTGCCGCAATTGGTGCGGTAACCGGGGTGATTGTAGCGA8730.22351432104644642No Hit
AAAGTAGCATGGGGTTCCATCCCTGATTCAGGAAGATCCCACATGCCTCGGGACAACTA8160.20892060249014924No Hit
ATGCTAGCATGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTCCCAAGAA8030.20559221053871304No Hit
GGAGTAGCATGGGGGACCGGCCCTGAGCTGCCCTCGGGATCAAGCCAGCCCGATGTCCA7990.20456808993827114No Hit
GGAGTAGCATGGGGGTTGACAGAGTGAGACTGTCTCAAAAAACTAAAAACTAAAAAAAC7940.20328793918771873No Hit
AGTCTAGCATGGGGGAATTCGCCCTAAGTTCCGGTCCGCAATTTCGTTTCGTTGGGACG7620.1950949743841835No Hit
GGCTTAGCATGGGCAACAAGGCGAAACCCCGTCTCTACAAAATATAGAAAAATTAGCCA7360.18843819048131108No Hit
GCCTTAGCATGGGGTTTTCAACAACTTCACGGAAGAATAGGTTCGTGGAGCTCCTCATG6950.17794095432678153No Hit
GAGTTAGCATGGGCTCTCAGACCACAGTGCAATCAAACTAGAAGTCAGGATTAAGAATC6740.17256432117446152No Hit
GAATTAGCATGGGGTAGCTCTATGGAAAAAGACTATTTGAGAATTAGTAGTACTGTATT6680.17102814027379865No Hit
CTGTTAGCATGGGTGAGGAAACAGGTACAGAGAAGTTATAGGGCTTATATAAAGTTAGT6620.16949195937313577No Hit
AACATAGCATGGGGTTTGCAGGCTTAACTCCGGCCGGGGTTCCTCGCTCGAGCTACGAG6600.16897989907291483No Hit
TTTATAGCATGGGGTTGCTTAGCATGGGCAGATAATTTTTATGGCGAAACCCTGTCTCT6570.1682118086225834No Hit
TCGGTAGCATGGGTACATAAACACCTATAGATATAATTAGGTATAAATCTAACAATTAT6460.16539547697136814No Hit
CTAGTAGCATGGGGATAAACGCTATCACATTGTTGAAGGCGCAAACCATATGGATCTTT6440.1648834166711472No Hit
TAAGTAGCATGGGATGGTGGCATTTACGAAACGGGTGATCGCGAATATACATTGGATGA6260.16027487396915863No Hit
CCCATAGCATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGA6190.15848266291838528No Hit
GATCTAGCATGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGG5770.14772939661374523No Hit
GATCTAGCATGGGTCTCATAGCATGGGTAGGAGGAGTTGCGAACAAGCAGCAAGACCCC5720.14644924586319283No Hit
GGGTTAGCATGGGAAGTGCTCGCCGAACAGAACGCAGATGTCCGCCGCGATCCTGCCAG5710.1461932157130824No Hit
GAACTAGCATGGGGGGGAATTCAGATGTATATATGATGTCCTTGGACGGGGTGGCGCAG5710.1461932157130824No Hit
GGTCTAGCATGGGGTCCATTTTTTTAAGAGCAGAATGCTTTCTGGAAATGATGTATGAC5620.14388894436208807No Hit
ATTATAGCATGGGGGGTTCAAATGACTCTCCTGCCTCAGCCTCCTGAGTAGTTTGGATT5190.13287964790733758No Hit
ATTCTAGCATGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTG5100.13057537655634327No Hit
TCTTTAGCATGGGGGAGCTCAAAGAATGCAACAATATATAAACAAATCATCACACTAAA5090.1303193464062328No Hit
TGCGTAGCATGGGGTACAGCAGCTGCGCGTCTTTACGCACCACAAAGTGGGTGAAAACG5080.13006331625612233No Hit
ACATTAGCATGGGGTCTCTCTTCTTCTCATGAATGTTGATTACATGGTCATATTTGTGT5000.12801507505523851No Hit
TGGATAGCATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGA4860.12443065295369184No Hit
AAATTAGCATGACCTCACCAAATGAACTAAAGAAACCACCAGAGACCAGTCCTAGAGAA4740.1213582911523661No Hit
TATGTAGCATGGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATGCACAGC4700.12033417055192419No Hit
GACTTAGCATGGATGAAATCAACACACAGCTTTCCACTGTCATTCGCCATAGAAGCCGA4610.1180298992009299No Hit
GGATTAGCATGGGCAGAGTCGCCTAGCATGGGCCAAATCAGTAAATTTGTGCTACCATG4600.11777386905081942No Hit
GTACTAGCATGGGATCATGTCACTTTCTCAGATGTTTCCTCATCTGTCAAATTGAGTAA4580.11726180875059847No Hit
AATATAGCATGGGGACGTGTCAGTACTATGTGTGAGTGCTCCGCCCTAGTGCTGATGTA4530.11598165800004608No Hit
TTATTAGCATGGGTGTTGCATGATGCTAAGGTTTGGGCTTCCATTGATCCCGTCACCCA4510.11546959769982512No Hit
TTAGTAGCATGGGATGCCTAGATGTGATACACGTTTCTGGAAACTGCCTCGTCATGCGA4400.1126532660486099No Hit
ATCGTAGCATGGGCACACAGCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTGAA4200.10753266304640034No Hit
GAATTAGCATGGGGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGCTTG4140.10599648214573748No Hit
AAACTAGCATGGGGGTGCAGGGATGGTGTTTTCAGTCTTCTTTCATGAGTGAGAATAAA4140.10599648214573748No Hit
GTCGTAGCATGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTAGCAT4130.105740451995627No Hit
TTTGTAGCATGGGAGTAATGGCGGTGGTGAGCTCGGTGGTGCAGCTGGAGTCGCAGCAC4080.10446030124507462No Hit
AAGGTAGCATGGCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTC4020.10292412034441176No Hit
ACTGTAGCATGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGCTCACAGT3960.10138793944374891No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTGCT150.006692971653.00000453
TCCGGCC550.053.00000429
AACGTTT150.006692971653.00000427
ATTTACG550.053.00000422
CTTAACT550.053.00000423
GTCATGC650.053.00000451
TTAGGTG1100.053.00000417
AGACTTA150.006692971653.00000432
CGGGTGA550.053.00000432
TGTCAAT150.006692971653.00000432
CTAGAAG650.053.00000438
AGACTAA150.006692971653.00000420
ACTATCT1950.053.00000428
GATACCT150.006692971653.00000413
GTCGCGT150.006692971653.00000441
AATCCCA2200.053.00000420
GCCTTAT150.006692971653.00000415
AGTCCCG150.006692971653.00000434
CAAACCT150.006692971653.00000423
CAAACCG150.006692971653.00000443
Produced by FastQC (version 0.11.5)