FastQCFastQC Report
Fri 17 Jun 2016
SRR1528161_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528161_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2002693
Sequences flagged as poor quality0
Sequence length52
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTCAAAGTGGGGAGGATAACTGATGCATCACCATCATCACCATGCTCCTG45220.2257959657321417No Hit
GTGGCAAAGTGGGCTGATGAGCCAGTCCTGCTTGAAGAACCAGTAGTCTTGG38960.19453805450960282No Hit
TACGCAAAGTGGGGGGGCTGGTGAGATGGCTCAGTGGTTTAGAGCACCCGAC32740.16347987434918881No Hit
TCATCAAAGTGGGCGGGTGCAGTGGCTCACACTTGTTATCCCAGCTCTTTGG30620.15289412805657182No Hit
TCGTCAAAGTAGGCATCAAAAAAAATGAAGAAGAACTCTAGATAATGTAGCC28370.14165925581204908No Hit
GTTTCAAAGTGGGGAGGATAACTGATGCATCACCATCATCACCATGCTCCTG26430.1319722992989939No Hit
GCTCCAAAGTGGTGTTAACGCAGAGTTATTCAAAGTGGGGCTACTTGGGAGG24760.12363352745528146No Hit
GGTGCAAAGTGGGGATGCAGAGATGACCTGTGACTTGTAGGCAGTTAAAATT24400.12183594789615783No Hit
TGTACAAAGTGGGACGCGCGGCGACATGGAAGACATCCGCGGCGTGACGATC24030.11998843557150297No Hit
GGACCAAAGTGGGTGTATGACAGACACGTGTCTTTCCCCTAGGGAAGACATG23670.11819085601237932No Hit
CAGGCAAAGTGGGGGACTCGACTCGGTCCTCCGCCCGCCCCGGGAATACCTC23620.11794119218472326No Hit
AATCCAAAGTGGGAGACGCCGCCTCGGCGAGCCGGGCGCCGGGCAGGAACGC23300.11634334368772449No Hit
TGCTCAAAGTTGGGGTTGTGGCAATAGCCCAGTATGCAGTCACTTAGAAATT23100.11534468837710024No Hit
GATTCAAAGTGGGAGAGTGTGTCAAAGTGGGAAGCAGTATCAACGCAGAATC22940.11454576412860083No Hit
GTTCCAAAGTGGGCGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAAC22900.11434603306647599No Hit
AGCTCAAAGTGGACAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCC22170.11070094118269751No Hit
TGTTCAAAGTGGGGAGGATAACTGATGCATCACCATCATCACCATGCTCCTG21440.10705584929891901No Hit
AAATCAAAGTGGGGAGTCATCCAAAGTGGGTCATGTAATTTAATGTGCCAAA21180.10575759739510748No Hit
TCAGCAAAGTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAG21140.10555786633298263No Hit
CAGTCAAAGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTG20930.10450927825682718No Hit
TCAGCAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAA20800.10386015230492143No Hit
CTGACAAAGTGGGGCTTCCTGTCCCGGCCGCCATCGGAGAGCATCAGCCATG20770.1037103540083278No Hit
TTAGCAAAGTGGGGCAGTGGTATCAACGCAGCTACCTCTCCCCACGGGTGCA20710.10341075741514051No Hit
AGTTCAAAGTGGGGGCTGGAGAGGTGGCTCAGAGGTTTAGAGCACTGGCTGT20210.1009141191385799No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAATTT1500.046.00000412
CACATTT750.046.00000419
TTAGATA1550.046.00000431
GATCCCC1500.046.00000413
TTCACGA750.046.00000420
CCGTGTC1450.046.00000433
TTCACCG1500.046.00000425
CAACCGT1500.046.00000445
CCGTGAA1450.046.00000434
TGAACCT1550.046.00000421
ATGCTTA750.046.00000444
ATTAAGG750.046.00000444
TTCGTGT750.046.00000421
CTAAGTC1450.046.00000419
GTTAGAA750.046.00000433
TAAGTCC1450.046.00000420
ATGTCTA750.046.00000422
GTAATCT1500.046.00000437
ATGTAAC1500.046.00000444
TCTACGG750.046.00000417
Produced by FastQC (version 0.11.5)